Article
Microbiology
Dan-dan Li, Zheng Zhang, Jia-ning Wang, Peng Zhang, Ya Liu, Yue-zhong Li
Summary: Complex polysaccharides such as cellulose, xylan, and chitin are primarily degraded by microorganisms in nature, but their global distribution and responsible enzymes remain poorly understood. Through large-scale sequence alignments, it was found that almost all prokaryotic communities have the potential to degrade cellulose, xylan, and chitin. The abundance and diversity of these enzyme systems vary across different environments and taxonomic sources. Most genera with degradation potentials are narrowly distributed in specific communities. This study provides a preliminary panorama of cellulose-, xylan-, and chitin-degrading enzymatic systems across global prokaryotic communities.
ENVIRONMENTAL MICROBIOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Dong Tian, Yiyi Chen, Fei Shen, Maoyuan Luo, Mei Huang, Jinguang Hu, Yanzong Zhang, Shihuai Deng, Li Zhao
Summary: The study revealed that the PHP pretreatment system can self-generate peroxyacetic acid oxidant, effectively mediating lignocellulose deconstruction and hemicellulose/lignin degradation. Good degradation results were observed on wheat straw and corn stalk with the assistance of peroxyacetic acid oxidation, achieving high recovery rates and digestibility.
BIOTECHNOLOGY FOR BIOFUELS
(2021)
Article
Agricultural Engineering
Italo Pisano, Lalitha Gottumukkala, Daniel J. Hayes, James J. Leahy
Summary: Accurate composition analysis of biomass is essential in determining its potential uses and processing methods. This study focused on assessing lignocellulosic constituents in forestry, agricultural, and underutilised waste residues, with emphasis on hemicellulose monomers. Variations were observed in structural sugars content, hemicellulose concentration, and composition among different feedstock types and fractions.
INDUSTRIAL CROPS AND PRODUCTS
(2021)
Article
Chemistry, Multidisciplinary
Joao Paulo L. Franco Cairo, Fernanda Mandelli, Robson Tramontina, David Cannella, Alessandro Paradisi, Luisa Ciano, Marcel R. Ferreira, Marcelo Liberato, Livia B. Brenelli, Thiago A. Goncalves, Gisele N. Rodrigues, Thabata M. Alvarez, Luciana S. Mofatto, Marcelo F. Carazzolle, Jose G. C. Pradella, Adriana F. Paes Leme, Ana M. Costa-Leonardo, Mario Oliveira-Neto, Andre Damasio, Gideon J. Davies, Claus Felby, Paul H. Walton, Fabio M. Squina
Summary: Wood-feeding termites degrade plant biomass through enzymatic degradation, and a previously unknown redox-active enzyme called CgSOD-1 plays a key role in this process.
Article
Biochemistry & Molecular Biology
Ademola Hammed, Yehor Polunin, Andriy Voronov, Scott W. Pryor
Summary: In this study, cellulase enzymes were immobilized on polymer-enzyme conjugates (PECs) and recovered using a membrane, showing higher conversion rates and reduced impact on initial hydrolysis during biomass hydrolysis. The combination of PECs and membrane recovery has the potential to reduce hydrolysis costs during cellulosic bioprocessing, indicating promising implications for industrial applications.
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
(2021)
Article
Agriculture, Multidisciplinary
Ci Jin, Jie Bao
Summary: A preliminary study showed the feasibility of lysine production from lignocellulose, though the conversion of xylose in lignocellulose to lysine remains unsolved. By modifying the biorefinery processing and strain development, a new approach resulted in successful lysine production from lignocellulose, providing a practical method for future industrial applications.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2021)
Article
Engineering, Environmental
Diep Ngoc Pham, Anh Duc Nguyen, Dung Hoang Anh Mai, Eun Yeol Lee
Summary: Methylotuvimicrobium alcaliphilum 20Z was genetically modified to simultaneously consume methane and lignocellulose-derived sugars to produce ectoine. Cultivation on three substrates upregulated the Embden-Meyerhof-Parnas and ectoine biosynthesis pathways, resulting in enhanced ectoine production. Knockout of the ectoine biosynthesis repressor further increased ectoine yield.
CHEMICAL ENGINEERING JOURNAL
(2023)
Article
Chemistry, Applied
Maria Guadalupe Moran-Aguilar, Montserrat Calderon-Santoyo, Ricardo Pinheiro de Souza Oliveira, Maria Guadalupe Aguilar-Uscanga, Jose Manuel Dominguez
Summary: Biorefinery with deep eutectic solvent (DES) is an emerging processing technology that can overcome the limitations of conventional biomass pretreatments. This study evaluates the biorefinery of sugarcane bagasse (SCB) using DES formulated with choline chloride as hydrogen bond acceptor and three hydrogen bond donors: lactic acid, citric acid, and acetic acid. It was found that acetic acid has unique ionic properties that contribute to the selective removal of lignin and the deconstruction of cellulose, resulting in enhanced digestibility during enzymatic hydrolysis.
CARBOHYDRATE POLYMERS
(2022)
Article
Energy & Fuels
Xushen Han, Weitao Dong, Jie Bao
Summary: This study introduced a solid-state fermentation prior to steam pretreatment of corn stover to convert water-soluble carbohydrates into lactic acid, which improved the conversion yields of xylan and arabinan in the steam pretreatment and reduced the generation of toxic HMF.
BIOMASS CONVERSION AND BIOREFINERY
(2023)
Article
Biotechnology & Applied Microbiology
Mohamed Ali Abdel-Rahman, Saad El-Din Hassan, Hassan M. A. Alrefaey, Tamer Elsakhawy
Summary: This study aimed to design lignocellulosic substrates for efficient lactic acid production using a thermophilic, xylose-utilizing, and inhibitor-resistant Bacillus coagulans Azu-10 strain. The results showed that strain Azu-10 efficiently co-utilized different sugar mixtures without carbon catabolite repression, leading to high lactic acid production from lignocellulosic biomass.
FERMENTATION-BASEL
(2021)
Article
Environmental Sciences
Bishal Sharker, Md. Aminul Islam, Md. Al Amin Hossain, Iqrar Ahmad, Abdullah Al Mamun, Sibdas Ghosh, Aminur Rahman, Md. Shohorab Hossain, Md. Ashikujjaman Ashik, Md. Rayhanul Hoque, Md. Khalid Hossain, M. Al Mamun, Md. Atiqul Haque, Harun Patel, Md. Yeasin Prodhan, Prosun Bhattacharya, Md. Azizul Haque
Summary: This study characterized three bacterial strains isolated from cow rumen and forest soil, which showed the ability to effectively break down lignocellulose and enhance bioethanol production. Genomic analysis revealed that these strains secrete various enzymes that can interact with lignocellulosic substrates. Treatment with these strains resulted in increased crystallinity index and fermentable cellulose in rice straw. These findings suggest a novel model for rice straw deconstruction using the hydrolytic enzymes of these bacteria, which exhibits superior efficacy compared to individual strains and promotes the development of cost-effective, affordable, and sustainable green technology.
SCIENCE OF THE TOTAL ENVIRONMENT
(2023)
Article
Agronomy
Olga Monago-Maran, Nils Kristian Afseth, Svein Halvor Knutsen, Sileshi Gizachew Wubshet, Jens Petter Wold
Summary: This study demonstrates the feasibility of using Raman spectroscopy for quantification of soluble solids and individual sugars in apples, achieving high estimation accuracy with multivariate calibration models. Results show that identifiable Raman bands related to target sugars can be highlighted in regression coefficients for all calibration models, indicating the potential for accurate estimation of sugar content in apples using Raman spectroscopy.
POSTHARVEST BIOLOGY AND TECHNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Jian-Qiang Yu, Kai-Di Gu, Li-Li Zhang, Cui-Hui Sun, Quan-Yan Zhang, Jia-Hui Wang, Chu-Kun Wang, Wen-Yan Wang, Meng-Chi Du, Da-Gang Hu
Summary: This study reveals that the MdPFP beta gene regulates soluble sugar accumulation in apple by enhancing photosynthetic performance and sugar-metabolizing enzyme activities. Additionally, the transcription factor MdbHLH3 activates the expression of MdPFP beta gene, leading to increased photosynthetic rates and soluble sugar accumulation in apple.
JOURNAL OF INTEGRATIVE PLANT BIOLOGY
(2022)
Review
Microbiology
Justyna Ruchala, Andriy A. Sibirny
Summary: The paper discusses the distribution of pentose sugars in nature, as well as the metabolism and conversion processes of D-xylose and L-arabinose. It provides a detailed exploration of pentose metabolism and bioconversion in non-conventional yeasts, and compares pentose metabolism in recombinant strains. The article also reviews genome studies of natural xylose-metabolizing yeasts and the metabolism of other pentoses.
FEMS MICROBIOLOGY REVIEWS
(2021)
Article
Agricultural Engineering
Karol Dudek, German Buitron, Idania Valdez-Vazquez
Summary: This study demonstrated the successful solubilization of Agave bagasse fibers using acidogenesis process with native microbiota, resulting in increased VFA production, sCOD, and VFA yield. Nutrient supplementation promoted alkaline pH, leading to different VFA compositions and yields.
INDUSTRIAL CROPS AND PRODUCTS
(2021)
Article
Microbiology
Indrani Mukhopadhya, Sarah Morais, Jenny Laverde-Gomez, Paul O. Sheridan, Alan W. Walker, William Kelly, Athol V. Klieve, Diane Ouwerkerk, Sylvia H. Duncan, Petra Louis, Nicole Koropatkin, Darrell Cockburn, Ryan Kibler, Philip J. Cooper, Carlos Sandoval, Emmanuelle Crost, Nathalie Juge, Edward A. Bayer, Harry J. Flint
ENVIRONMENTAL MICROBIOLOGY
(2018)
Article
Biochemistry & Molecular Biology
Albert Galera-Prat, Sarah Morais, Yael Vazana, Edward A. Bayer, Mariano Carrion-Vazquez
JOURNAL OF BIOLOGICAL CHEMISTRY
(2018)
Article
Chemistry, Physical
Haim Yuval Aviram, Menahem Pirchi, Yoav Barak, Inbal Riven, Gilad Haran
JOURNAL OF CHEMICAL PHYSICS
(2018)
Article
Multidisciplinary Sciences
Haim Yuval Aviram, Menahem Pirchi, Hisham Mazal, Yoav Barak, Inbal Riven, Gilad Haran
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2018)
Article
Microbiology
Lior Artzi, Tali Dadosh, Elad Milrot, Sarah Morais, Smadar Levin-Zaidman, Ely Morag, Edward A. Bayer
Article
Biotechnology & Applied Microbiology
Johanna Stern, Sarah Morais, Yonit Ben-David, Rachel Salama, Melina Shamshoum, Raphael Lamed, Yuval Shoham, Edward A. Bayer, Itzhak Mizrahi
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2018)
Article
Chemistry, Physical
Raisa Kantaev, Inbal Riven, Adi Goldenzweig, Yoav Barak, Orly Dym, Yoav Peleg, Shira Albeck, Sarel J. Fleishman, Gilad Haran
JOURNAL OF PHYSICAL CHEMISTRY B
(2018)
Review
Biochemistry & Molecular Biology
Sarah Morais, Itzhak Mizrahi
TRENDS IN MICROBIOLOGY
(2019)
Article
Biotechnology & Applied Microbiology
Amaranta Kahn, Sarah Morais, Anastasia P. Galanopoulou, Daehwan Chung, Nicholas S. Sarai, Neal Hengge, Dimitris G. Hatzinikolaou, Michael E. Himmel, Yannick J. Bomble, Edward A. Bayer
BIOTECHNOLOGY FOR BIOFUELS
(2019)
Article
Multidisciplinary Sciences
Hisham Mazal, Marija Iljina, Yoav Barak, Nadav Elad, Rina Rosenzweig, Pierre Goloubinoff, Inbal Riven, Gilad Haran
NATURE COMMUNICATIONS
(2019)
Article
Chemistry, Physical
Arina Dalaloyan, Andrea Martorana, Yoav Barak, Diana Gataulin, Eitan Reuveny, Andrew Howe, Michael Elbaum, Shira Albeck, Tamar Unger, Veronica Frydman, Elwy H. Abdelkader, Gottfried Otting, Daniella Goldfarb
Article
Environmental Sciences
Michal Pardo, Fanfan Xu, Michal Shemesh, Xinghua Qiu, Yoav Barak, Tong Zhu, Yinon Rudich
SCIENCE OF THE TOTAL ENVIRONMENT
(2019)
Article
Multidisciplinary Sciences
Angeliki Giannoulis, Akiva Feintuch, Yoav Barak, Hisham Mazal, Shira Albeck, Tamar Unger, Feng Yang, Xun-Cheng Su, Daniella Goldfarb
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2020)
Article
Biotechnology & Applied Microbiology
Yonit Ben-David, Sarah Morais, Edward A. Bayer, Itzhak Mizrahi
MICROBIAL BIOTECHNOLOGY
(2020)
Article
Biology
Meltem Tatli, Sarah Morais, Omar E. Tovar-Herrera, Yannick J. Bomble, Edward A. Bayer, Ohad Medalia, Itzhak Mizrahi
Summary: Microbes live and operate at micron and nanoscales, and studying them at these resolutions is crucial for understanding their ecology. The cellulose degradation process reveals how microbes build and utilize cellulosomal machinery at nanometer scales. Bacterial cells form "cellulosome capsules" driven by catalytic product-dependent dynamics, increasing the hydrolysis rate. Biosynthesis of this energy-intensive machinery and cell growth are decoupled at the single-cell level, suggesting a division-of-labor strategy through phenotypic heterogeneity. This study highlights the importance of intrapopulation interactions in understanding rates of fiber degradation.