Article
Polymer Science
Siyu Lu, Fan Li, Bo Liu, Kun Yang, Feng Tian, Zhi Cheng, Sheng Ding, Kexin Hou, Ian Wyman
Summary: Staphylococcus aureus is a common cause of hospital-acquired infections and foodborne illnesses and is widely present in nature. In this study, highly monodisperse fluorescent polystyrene microspheres were prepared, which exhibited similar fluorescence spectra and particle size distribution to SA aerosols. The fluorescence characteristics of the microspheres remained similar even after 180 days, providing a new idea for analogs of SA aerosols.
Article
Chemistry, Medicinal
Tsung-Shing Andrew Wang, Pin-Lung Chen, Yi-Chen Sarah Chen, Hsuan-Min Hung, Jhih-Yi Huang
Summary: A dual fluorescent probe scheme was designed to selectively target and differentiate between pathogenic and harmful vancomycin-resistant S. aureus (VRSA) and vancomycin-sensitive S. aureus (VSSA), by combining siderophores and antibiotics as guiding units in complex bacterial samples.
ACS INFECTIOUS DISEASES
(2021)
Article
Chemistry, Analytical
Rui Liu, Suleiman A. Haruna, Shujat Ali, Jing Xu, Yunlian Zhang, Peng Lu, Huanhuan Li, Quansheng Chen
Summary: A fluorescent genosensor was developed for Staphylococcus aureus detection, utilizing interactions between specific single-stranded DNA and graphene oxide quantum dots for sensitive detection. The sensor exhibited high sensitivity with a LOD of 0.98 x 10(-17) mol L-1 and was successfully applied for real sample analysis. Additionally, the sensor could be adapted for the detection of other pathogens by replacing recognition probes and primers.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Article
Multidisciplinary Sciences
Aisling M. Towell, Cecile Feuillie, Pauline Vitry, Thaina M. Costa, Marion Mathelie-Guinlet, Sanja Kezic, Orla M. Fleury, Maeve A. McAleer, Yves F. Dufrene, Alan D. Irvine, Joan A. Geoghegan
Summary: Staphylococcus aureus interacts with the host protein corneodesmosin on atopic dermatitis corneocytes, taking advantage of the aberrant display of corneodesmosin caused by low natural moisturizing factor levels. The bacterial proteins FnBPB and ClfB bind to the N-terminal region of corneodesmosin, facilitating strong adherence of S. aureus to atopic dermatitis corneocytes.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Biochemistry & Molecular Biology
Jie Peng, Qiangsheng Lu, Lvfeng Yuan, Hecheng Zhang
Summary: Mastitis caused by Staphylococcus aureus in dairy cows is difficult to treat due to drug-resistant strains. Novel lipopeptide antibiotics are important for treating bacterial diseases. This study synthesized three cationic lipopeptides and found that C16dKdK exhibited strong antibacterial activity against S. aureus and could safely treat mastitis in mice.
Article
Food Science & Technology
Xiaonan Zhao, Xiaomeng Yuan, Ming Hu, Yin Zhang, Lulu Li, Qing Zhang, Xuexia Yuan, Wenbo Wang, Yuqing Liu
Summary: The study revealed a relatively high prevalence of S. aureus in bulk tank milk from Shandong dairy farms, with the isolates carrying multiple virulence genes and exhibiting multiple antimicrobial resistance. The lurking threat of MRSA in raw milk emphasizes the need for surveillance programs starting at the farm level.
Article
Engineering, Biomedical
Vittoria Civilini, Vincenzo Giacalone, Alberto L. Audenino, Mara Terzini
Summary: Despite the lack of specific standards, this study provides a rigorous test protocol for mechanical comparison of surgical meshes used in hernia repair and urogynecologic surgery. The protocol consists of three quasi-static test methods and post-processing procedures to compute mechanical parameters. The test protocol was successfully applied to various meshes and showed intra-subject variability with coefficient of variations around 0.05, demonstrating its repeatability.
JOURNAL OF THE MECHANICAL BEHAVIOR OF BIOMEDICAL MATERIALS
(2023)
Article
Infectious Diseases
Madeeha Afzal, Ajay Kumar Vijay, Fiona Stapleton, Mark D. P. Willcox
Summary: Staphylococcus aureus isolates exhibited varying levels of resistance to antibiotics and disinfecting solutions, with microbial keratitis strains commonly showing resistance to multiple drugs. Strains from Australia were generally more susceptible to antibiotics compared to those from the USA, highlighting regional differences in resistance patterns.
Article
Chemistry, Analytical
Honglin Yang, Jinxia Xue, Jizhou Li, Guangxu Hu, Hongtao Li, Shuguang Lu, Zhifeng Fu
Summary: In this study, a bifunctional protein was successfully expressed, which can bind to MRSA and emit fluorescence, making it a potential tool for rapid diagnosis and antibiotic susceptibility testing.
ANALYTICA CHIMICA ACTA
(2022)
Article
Microbiology
Nilton A. S. Neto, Jose T. A. Oliveira, Tawanny K. B. Aguiar, Leandro P. Bezerra, Levi A. C. Branco, Felipe P. Mesquita, Cleverson D. T. Freitas, Pedro F. N. Souza
Summary: This study investigated the antibiofilm activity of synthetic antimicrobial peptides (SAMPs) in combination with the antibiotic ciprofloxacin against Staphylococcus aureus. The results demonstrated that certain peptides in combination with ciprofloxacin effectively inhibited biofilm formation and degraded preformed biofilms. These active peptides formed pores in the bacterial cellular membrane, enhancing the antibacterial activity of ciprofloxacin.
Article
Immunology
Kristina D. D. Hinman, Sonia S. S. Laforce-Nesbitt, Joshua T. T. Cohen, Miles Mundy, Joseph M. M. Bliss, Alexander R. R. Horswill, Craig T. T. Lefort
Summary: Techniques for studying bacterial infection clearance are critical for understanding diseases, immune cell functions, and antimicrobial therapeutics. This study develops a bi-fluorescent assay to monitor Staphylococcus aureus killing by neutrophils in vivo. The use of a stable secondary fluorescent marker allows for the separation of infected cell samples and evaluation of neutrophil function in a murine model of chronic granulomatous disease.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Infectious Diseases
Jasmina Al Janabi, Staffan Tevell, Raphael Niklaus Sieber, Marc Stegger, Bo Soderquist
Summary: This study investigated the susceptibility of Staphylococcus epidermidis strains from prosthetic joint infections to dalbavancin and the genomic variation in strains with reduced susceptibility. The results suggest that exposure to dalbavancin may lead to reduced susceptibility through selection of pre-existing subpopulations, epigenetic changes, or spontaneous mutations during antibiotic exposure. Therefore, source control and adequate antibiotic concentrations are important to prevent emerging reduced susceptibility during dalbavancin treatment.
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2023)
Article
Infectious Diseases
Maya Azrad, Chen Shmuel, Tamar Leshem, Zohar Hamo, Moti Baum, Assaf Rokney, Keren Agay-Shay, Avi Peretz
Summary: This study investigated the prevalence of chlorhexidine gluconate (CHG) tolerance among S. aureus strains in Israel, finding 13.15% of isolates to be CHG-tolerant. An association between phenotypic and genotypic tolerance to CHG was observed, with phenotypic tolerance being associated with methicillin resistance but not with mupirocin resistance. Most CHG-tolerant strains were isolated from blood cultures, indicating their prevalence in invasive infections. Further research is needed to evaluate risk factors for the development of CHG tolerance.
Article
Medicine, General & Internal
Hae-Sun Chung, Miae Lee
Summary: The methods and results obtained using commercialized automation systems for antimicrobial susceptibility testing are inconsistent. This study evaluated different testing methods and found that BMD and Etest were more reliable compared to VITEK2, Sensititre, Phoenix, and MicroScan in determining vancomycin susceptibility and minimum inhibitory concentration for SA-RVS.
Article
Biology
Salim Manoharadas, Mohammad Altaf, Abdulwahed Fahad Alrefaei, Naushad Ahmad, Shaik Althaf Hussain, Basel F. Al-Rayes
Summary: Researches have successfully designed an engineered antimicrobial protein to combat methicillin-resistant Staphylococcus aureus, showing strong activity. However, despite having a multimodular structure, the engineered protein failed to exhibit activity against a veterinary isolate of S. aureus.
Article
Biochemical Research Methods
Ann-Marie Broome, Gopal Ramarnurthy, Kari Lavik, Alexander Liggett, Ian Kinstlinger, James Basilion
BIOCONJUGATE CHEMISTRY
(2015)
Article
Medicine, Research & Experimental
Suraj Dixit, Kayla Miller, Yun Zhu, Emilie McKinnon, Thomas Novak, Malcolm E. Kenney, Ann-Marie Broome
MOLECULAR PHARMACEUTICS
(2015)
Article
Chemistry, Multidisciplinary
Suraj Dixit, Thomas Novak, Kayla Miller, Yun Zhu, Malcolm E. Kenney, Ann-Marie Broome
Article
Multidisciplinary Sciences
Ronen Durst, Kimberly Sauls, David S. Peal, Annemarieke deVlaming, Katelynn Toomer, Maire Leyne, Monica Salani, Michael E. Talkowski, Harrison Brand, Maelle Perrocheau, Charles Simpson, Christopher Jett, Matthew R. Stone, Florie Charles, Colby Chiang, Stacey N. Lynch, Nabila Bouatia-Naji, Francesca N. Delling, Lisa A. Freed, Christophe Tribouilloy, Thierry Le Tourneau, Herve LeMarec, Leticia Fernandez-Friera, Jorge Solis, Daniel Trujillano, Stephan Ossowski, Xavier Estivill, Christian Dina, Patrick Bruneval, Adrian Chester, Jean-Jacques Schott, Kenneth D. Irvine, Yaopan Mao, Andy Wessels, Tahirali Motiwala, Michel Puceat, Yoshikazu Tsukasaki, Donald R. Menick, Harinath Kasiganesan, Xingju Nie, Ann-Marie Broome, Katherine Williams, Amanda Johnson, Roger R. Markwald, Xavier Jeunemaitre, Albert Hagege, Robert A. Levine, David J. Milan, Russell A. Norris, Susan A. Slaugenhaupt
Review
Neurosciences
Amy Lee Bredlau, Suraj Dixit, Chao Chen, Ann-Marie Broome
CURRENT NEUROPHARMACOLOGY
(2017)
Article
Biotechnology & Applied Microbiology
Kayla Miller, Suraj Dixit, Amy-Lee Bredlau, Alfred Moore, Emilie McKinnon, Ann-Marie Broome
Article
Pharmacology & Pharmacy
Amy Lee Bredlau, Anjan Motamarry, Chao Chen, M. A. McCrackin, Kris Helke, Kent E. Armeson, Katrina Bynum, Ann-Marie Broome, Dieter Haemmerich
Article
Cell Biology
Joy N. Jones Buie, Yue Zhou, Andrew J. Goodwin, James A. Cook, John Vournakis, Marina Demcheva, Ann-Marie Broome, Suraj Dixit, Perry V. Halushka, Hongkuan Fan
Article
Chemistry, Multidisciplinary
Peng Zhu, Carl Atkinson, Suraj Dixit, Qi Cheng, Danh Tran, Kunal Patel, Yu-Lin Jiang, Scott Esckilsen, Kayla Miller, Grace Bazzle, Patterson Allen, Alfred Moore, Ann-Marie Broome, Satish N. Nadig
Article
Oncology
Leilei Zhang, Jie Zhang, Zhiwei Ye, Yefim Manevich, Lauren E. Ball, Jennifer R. Bethard, Yu-Lin Jiang, Ann-Marie Broome, Annamarie C. Dalton, Gavin Y. Wang, Danyelle M. Townsend, Kenneth D. Tew
Article
Chemistry, Multidisciplinary
Yu Lin Jiang, Ann-Marie Broome
Article
Oncology
Denise Garcia, Patrick Nasarre, Ingrid Bonilla, Eleanor Hilliard, Yuri K. Peterson, Laura Spruill, Anne-Marie Broome, Elizabeth G. Hill, Jason T. Yustein, Shikhar Mehrotra, Nancy Klauber-DeMore
ANNALS OF SURGICAL ONCOLOGY
(2019)
Article
Biochemistry & Molecular Biology
Joyce Nair-Menon, Amanda C. Daulagala, Dean M. Connor, Lauren Rutledge, Trevor Penix, Mary Catherine Bridges, Bridgette Wellslager, Demetri D. Spyropoulos, Cynthia D. Timmers, Ann-Marie Broome, Antonis Kourtidis
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2020)
Article
Pharmacology & Pharmacy
Leilei Zhang, Danyelle M. Townsend, Morgan Morris, Eduardo N. Maldonado, Yu-Lin Jiang, Ann-Marie Broome, Jennifer R. Bethard, Lauren E. Ball, Kenneth D. Tew
JOURNAL OF PHARMACOLOGY AND EXPERIMENTAL THERAPEUTICS
(2020)
Article
Biochemistry & Molecular Biology
Yu Lin Jiang, Yun Zhu, Alfred B. Moore, Kayla Miller, Ann-Marie Broome
ACS CHEMICAL NEUROSCIENCE
(2018)