Article
Biochemical Research Methods
Suzanne L. Parker, Steven C. Wallis, Cheryl Fourie, Melissa Lassig-Smith, Therese Starr, Avinash Chikatamarla, Tavey Dorofaeff, Mark D. Chatfield, Jeffrey Lipman, Jason A. Roberts
Summary: Reducing the volume of blood sampled from neonatal or paediatric patients is crucial for research purposes, with skin prick and capillary microsamples producing comparable results to conventional arterial blood sampling in terms of meropenem concentrations. CMS samples have been found to be stable when stored in the capillary tube for certain durations.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Antoni Kowalski, Cristine Betzer, Sigrid Thirup Larsen, Emil Gregersen, Estella A. Newcombe, Montana Caballero Bermejo, Viktor Wisniewski Bendtsen, Jorin Diemer, Christina Ernstsen, Shweta Jain, Alicia Espina Bou, Annette Eva Langkilde, Lene N. Nejsum, Edda Klipp, Robert Edwards, Birthe B. Kragelund, Poul Henning Jensen, Poul Nissen
Summary: This study shows that monomeric alpha-synuclein can activate plasma membrane Ca2+-ATPase (PMCA) to promote calcium clearance in neurons. The colocalization of alpha-synuclein and PMCA at neuronal synapses suggests a potential physiological function for alpha-synuclein in regulating calcium expulsion.
Article
Biochemistry & Molecular Biology
Chen-Chih Chung, Lung Chan, Jia-Hung Chen, Yi-Chieh Hung, Chien-Tai Hong
Summary: Plasma extracellular vesicle (EV) alpha-synuclein levels were significantly lower in patients with Parkinson's disease (PD) compared to controls, and were associated with the severity of akinetic-rigidity symptoms in PD patients. This study supports the diagnostic potential and clinical relevance of plasma EV alpha-synuclein as a biomarker for PD subtyping.
Article
Chemistry, Analytical
Long Yuan, Di Jiang, Andrew Pinkham, Melissa Kirkland
Summary: Capillary microsampling is a preferred technique for blood collection in rats and mice. A validated CMS LC-MS/MS assay for the quantification of BIIB131 in rat plasma has been developed and successfully applied to a safety study in rats.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2023)
Article
Biochemical Research Methods
Long Yuan, Jean-Francois Dupuis, Valerie Vrionis, Kevork Mekhssian, Tom Magee
Summary: This study is the first to report the use of capillary microsampling (CMS) for quantitative bioanalysis of antisense oligonucleotides (ASOs) using liquid chromatography-tandem mass spectrometry. The developed method was successfully validated and applied in a safety study in mice, demonstrating equivalent performance between CMS samples and conventional samples. The utility of CMS strategy has further been applied to other ASOs.
Article
Biochemical Research Methods
Yarmarly C. Guerra Valero, Tavey Dorofaeff, Jason A. Roberts, Jeffrey Lipman, Mark G. Coulthard, Louise Sparkes, Steven C. Wallis, Suzanne L. Parker
Summary: Critical illness affects the pharmacokinetics of antibiotics, leading to potential ineffective exposure and antibiotic resistance. Lack of studies on antibiotic pharmacokinetics in critically ill children results in significant off-label dosing issues. A bioanalytical method using capillary microsampling was developed and validated for the quantification of cefotaxime and its metabolite in plasma samples from critically ill children.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2021)
Article
Biochemistry & Molecular Biology
Samah Al-Harthi, Vladlena Kharchenko, Papita Mandal, Spyridon Gourdoupis, Lukasz Jaremko
Summary: Metal ions in cellular microenvironment are known to drive the aggregation of amyloid forming proteins. This study reveals the atomic details of zinc (Zn2+) binding to alpha-synuclein (AS) and its role in enhancing the anti-aggregation function of human serum albumin (HSA), shedding light on the previously undocumented role of Zn2+ in AS aggregation and HSA function.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Article
Neurosciences
Qi Zhang, Zenan Lin, Yan He, Junhong Jiang, Di Hu
Summary: The studies exploring plasma alpha-synuclein as a biomarker of Parkinson's disease have conflicting results. This study used the Mendelian randomization approach to investigate the causal relationship between plasma alpha-synuclein and PD. Despite observing a positive effect of plasma alpha-synuclein on PD risk, the results did not support a causal relationship. The findings were robust across sensitivity analyses.
MOLECULAR NEUROBIOLOGY
(2023)
Correction
Chemistry, Multidisciplinary
Esmaeil Darvish Aminabad, Ahmad Mobed, Mohammad Hasanzadeh, Mohammad Ali Hosseinpour Feizi, Reza Safaralizadeh, Farzad Seidi
Summary: This study presents a modification of an immunosensing method using gold nanoparticles conjugated with graphene for sensitive detection of α-synuclein protein in human plasma samples. The method shows potential for early identification of Parkinson's disease and can be used for point-of-care analysis.
Article
Biochemical Research Methods
Shoraku Ryu, Yoshiharu Hayashi, Shigehiro Yagishita, Ako Takahashi, Akira Yokoi, Mayu Ohuchi, Akinobu Hamada
Summary: This study developed a sensitive analytical method to measure E7130 concentration in mouse plasma samples obtained via microsampling. The UHPLC-HRMS method showed good precision and accuracy in E7130 quantitation, making it a valuable approach for pharmacokinetic analysis and quantitation of compounds.
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
(2022)
Article
Clinical Neurology
Anne Stuendl, Tanja Kraus, Madhurima Chatterjee, Bjoern Zapke, Boguslawa Sadowski, Wiebke Moebius, Markus A. Hobert, Christian Deuschle, Kathrin Brockmann, Walter Maetzler, Brit Mollenhauer, Anja Schneider
Summary: This study aimed to examine the diagnostic value of alpha-synuclein in plasma extracellular vesicles from patients with Parkinson's disease (PD). Results showed that alpha-synuclein concentration in plasma extracellular vesicles could provide good discrimination between different neurodegenerative diseases, suggesting its potential as a diagnostic biomarker for PD. The study highlights the importance of extracellular vesicle-derived alpha-synuclein in disease pathology and diagnosis.
MOVEMENT DISORDERS
(2021)
Article
Neurosciences
Don Gueu Park, Juhee Kang, Young-Sil An, Jaerak Chang, Jung Han Yoon
Summary: In early Parkinson's disease patients, plasma alpha-synuclein levels were found to correlate with cardiac sympathetic denervation but not with nigrostriatal degeneration. This suggests that plasma alpha-synuclein levels may more readily reflect the peripheral deposition of Lewy bodies rather than their central deposition.
NEUROSCIENCE LETTERS
(2022)
Article
Neurosciences
Hengxing Zheng, Zhenhua Xie, Xuran Zhang, Jian Mao, Mengyuan Wang, Sijia Wei, Yiwen Fu, Hong Zheng, Ying He, Hui Chen, Yan Xu
Summary: This study examined the aggregation status, localization, and degradation characteristics of a-syn and p-a-syn in plasma exosomes from PD patients and healthy controls. Results indicated poor solubility of a-syn and p-a-syn after protease K treatment, with aggregates found inside and on the membrane surface of plasma exosomes. The ratios of oligomeric a-syn/total a-syn and oligomeric p-a-syn/total p-a-syn in plasma exosomes showed moderate utility in assisting PD diagnosis.
Article
Engineering, Electrical & Electronic
Ming-Jing Lee, Yu-Jen Chang, Che-Jung Cheng, Yi-Chun Yeh, Chien-Fu Chen, Yi-Ju Chou
Summary: The separation of blood plasma by centrifugation can be accelerated by tilting the sample relative to the direction of the centrifugal force. This is due to the Boycott effect and buoyancy-induced convection, resulting in enhanced sedimentation of red blood cells. Flow instability invalidates the traditional predictive model, but our new model accounts for these factors and predicts the optimal tilt angle for highest separation efficiency. Experimental data confirms the accuracy of our theoretical prediction, and sensitivity analysis investigates the influence of blood sample variation on physical parameters.
SENSORS AND ACTUATORS A-PHYSICAL
(2023)
Article
Chemistry, Analytical
Jonas Carneiro Cruz, Mariana Azevedo Rosa, Lucas Mores, Eduardo Carasek, Jose Alexandre de Souza Crippa, Eduardo Costa Figueiredo, Maria Eugenia Costa Queiroz
Summary: This manuscript describes the development of magnetic restricted-access carbon nanotubes as a sorbent for determining CBD and THC in human plasma samples. The method showed adequate selectivity, linearity, precision, and accuracy. It was successfully applied to determine CBD levels in healthy volunteers and THC levels in cannabis smokers.
ANALYTICA CHIMICA ACTA
(2022)
