Article
Engineering, Environmental
Miaorong Zhang, Yan Zhang, Chuankai Yang, Chunyun Ma, Jianguo Tang
Summary: Enzyme-inorganic hybrid nanoflowers (HNFs) are a novel hybrid nanomaterial with superior performances, involving classification, synthesis, functionalization, and various potential application areas. The focus of the review is on developing classification, synthesis methods and applications of enzyme-inorganic HNFs in the field of biocatalysis. Current problems and prospects for further study are proposed.
CHEMICAL ENGINEERING JOURNAL
(2021)
Review
Multidisciplinary Sciences
Suliman Khan, Mohammad Mahdi Nejadi Babadaei, Anwarul Hasan, Zehra Edis, Farnoosh Attar, Rabeea Siddique, Qian Bai, Majid Sharifi, Mojtaba Falahati
Summary: This study explores the potential applications of enzyme-nanoparticle bio-conjugates in medical activities, providing hope for therapeutic enzymes immobilized on polymers, inorganic metal oxides, and hybrid compounds, and evaluating the use of ENBs in bioassay activities.
JOURNAL OF ADVANCED RESEARCH
(2021)
Article
Engineering, Chemical
Shan Qiao, Mingmin Li, Jiangyue Yu, Sainan Zhang, Dong Yan, Zhenjie Zhang, Yao Chen
Summary: By precisely adjusting the microenvironments to control the hydrophobicity and mass transfer of COF microcapsules, the bioactivity of the composite material can be improved.
Article
Biochemistry & Molecular Biology
Chan Hee Lee, Hye Sun Lee, Jae Won Lee, Jangyong Kim, Jin Hyung Lee, Eon Seon Jin, Ee Taek Hwang
Summary: Enzyme immobilization using inorganic materials, such as CaCO3, has been shown to improve enzyme stability and activity. In this study, in situ and crosslink-immobilized enzymes in a CaCO3 carrier were compared, with both methods preserving over 30% of initial enzyme activity after 180 days. However, differences in enzyme loading, activity, and characteristics were observed between the two methods.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Multidisciplinary Sciences
Khashayar Vojdanitalab, Hossein Jafari-Nodoushan, Somayeh Mojtabavi, Mahtab Shokri, Hoda Jahandar, Mohammad Ali Faramarzi
Summary: A novel concentrated method was developed for the instant fabrication of laccase@Co-3(PO4)(2)center dot hybrid nanoflowers. The synthesized nanoflowers exhibited excellent catalytic efficiency and stability, making them promising biocatalysts and pollutant removal agents.
SCIENTIFIC REPORTS
(2022)
Article
Chemistry, Physical
Yuxiao Feng, Yingjie Du, Geling Kuang, Le Zhong, Hongtong Hu, Shiru Jia, Jiandong Cui
Summary: This study reports a new hierarchical micro-mesoporous zeolitic imidazolate framework-8 (ZIF-8) with core-shell superstructure (HZIF-8) for enzyme immobilization. The immobilized enzyme in HZIF-8 exhibited higher activity and substrate affinity compared to conventional microporous ZIF-8, showing improved stability and reusability. The obtained HZIF-8 also showed outstanding performance in co-immobilization of multienzyme for glucose detection.
JOURNAL OF COLLOID AND INTERFACE SCIENCE
(2022)
Article
Biophysics
Yong Liu, Xinxin Shao, Deqiang Kong, Guangquan Li, Quanshun Li
Summary: Thermophilic lipase QLM from Alcaligenes sp. was successfully immobilized in Cu-3(PO4)2-based inorganic hybrid nanoflower through biomimetic mineralization. The immobilized enzyme exhibited enhanced stability and reusability compared with the free enzyme, making it a promising candidate for future biocatalytic applications. The optimization of temperature and pH conditions, as well as the protective effect of the nanoflower shell, were critical factors contributing to the favorable properties of the immobilized enzyme.
COLLOIDS AND SURFACES B-BIOINTERFACES
(2021)
Article
Chemistry, Analytical
Erkan Yilmaz, Zeliha Erbas, Mustafa Soylak
Summary: This study investigated the usability of hydrolytic enzyme immobilized magnetic nanoparticles for the microextraction of metal ions from food samples. By using alpha-amylase modified magnetic carbon nanotubes, the extraction of trace arsenic from food samples was achieved with high efficiency and reusability properties, overcoming challenges of enzyme activity recovery after extraction. The developed method showed promising results in terms of extraction efficiency, simplicity of operation, and reuse properties.
ANALYTICA CHIMICA ACTA
(2021)
Review
Biotechnology & Applied Microbiology
Zichen Wang, Ruirui Wang, Zixin Geng, Xiuyan Luo, Jiahui Jia, Saizhao Pang, Xianwei Fan, Muhammad Bilal, Jiandong Cui
Summary: Hybrid nanomaterials have emerged as a new interface of nanobiocatalysis, with inorganic crystal nanoflowers and metal-organic frameworks (MOFs) being of particular interest for enzyme immobilization. However, there is a lack of literature reviews on enzyme@MOF and enzyme-hybrid nanoflower composites and their synthesis strategies and applications in biotechnology. This review discusses innovative synthetic strategies and biotechnological applications of enzyme@MOF and enzyme-hybrid nanoflower composites, aiming to provide insights and ideas for future research.
CRITICAL REVIEWS IN BIOTECHNOLOGY
(2023)
Review
Chemistry, Inorganic & Nuclear
Jianxiong Chen, Zitao Guo, Yu Xin, Zhenghua Gu, Liang Zhang, Xuan Guo
Summary: This review article focuses on the classification, structure-function relationship, and applications of organic-inorganic hybrid nanoflowers (HNFs) in areas such as biocatalysis, biosensing, and medical diagnosis. The authors summarize the strategy for classifying HNFs based on their organic components and discuss the influence of the synthetic process on the properties of HNFs. They also explore the development of functionalized HNFs and their applications in industrial biocatalysis, environmental remediation, antibacterial properties, and biosensing. The potential of HNF-based biosensors for medical disease diagnosis and prevention is highlighted. The article concludes with an overview of the challenges and prospects for the further development of efficient and multi-functional HNF-based biocatalysts.
COORDINATION CHEMISTRY REVIEWS
(2023)
Article
Materials Science, Multidisciplinary
Hongde An, Bo Xie, Meng Ren, Zhuang Li, Wenchao Li, Yu Liu, Yuanzhuang Xu, Duqiang Luo
Summary: In this study, a self-activated nuclease formulation based on squaric acid-based MOFs was developed for efficient enzyme activation and protection. The MOFs improved the stability of the enzymes and released them during catalysis, resulting in high activity and resistance to harsh environments. This formulation also showed enhanced activity and stabilities for other nucleases.
ACS MATERIALS LETTERS
(2023)
Article
Chemistry, Multidisciplinary
Mengjuan Yang, Hao Cheng, Yuqiu Xu, Mengzhen Li, Yong Ai
Summary: Due to the diversity of structure and potential applications, lead halide perovskites have attracted great attention. In this study, a lead-based hybrid organic-inorganic perovskite material with nonlinear optical characteristics was prepared through halogen substitution. It showed good second harmonic generation response and switching endurance, making it suitable for optoelectronic application as a switching material.
CHINESE CHEMICAL LETTERS
(2022)
Review
Biotechnology & Applied Microbiology
Pravin D. Patil, Radhika K. Kelkar, Neha P. Patil, Pradnya V. Pise, Sadhana P. Patil, Arundhatti S. Patil, Nishant S. Kulkarni, Manishkumar S. Tiwari, Ajay N. Phirke, Shamraja S. Nadar
Summary: The use of organic-inorganic hybrid nanoflowers as support material for enzyme immobilization has become popular due to their stability, ease of preparation, and enhanced catalytic activity. However, their low density and high dispersion make them difficult to handle and separate. Magnetic nanoflowers have emerged as a promising alternative platform for enzyme immobilization due to their easy separation, structural stability, and ability to enhance catalytic efficiency. This review focuses on different methods for designing magnetic nanoflowers, examples of enzymes immobilized in magnetic nanoflowers, their applications, and ways to improve material properties for enhanced catalytic activity, structural stability, and scalability.
CRITICAL REVIEWS IN BIOTECHNOLOGY
(2023)
Article
Green & Sustainable Science & Technology
Angela Romano, Antonella Rosato, Grazia Totaro, Giulio Zanaroli, Annamaria Celli, Laura Sisti
Summary: The use of polymer-degrading enzymes in polymer formulation is an attractive strategy to improve the recyclability of plastics. This research focuses on the use of thermally stable enzymes as innovative materials to enhance the circularity of packaging materials. By immobilizing the cutinase enzyme in Mg/Al layered double hydroxide structures, its thermal stability is significantly improved, leading to efficient degradation of poly (butylene succinate-co-adipate) films.
JOURNAL OF CLEANER PRODUCTION
(2023)
Article
Multidisciplinary Sciences
Celia Arib, Jolanda Spadavecchia, Marc Lamy de la Chapelle
Summary: The study introduces a one-step synthesis method in which Manganese Superoxide Dismutase protein plays a key role in the reduction of gold salts, influencing the gold reduction mechanisms and integrating proteins directly into gold nanoparticles. This finding has wide applications, particularly in the green synthesis of novel nanomaterials.
SCIENTIFIC REPORTS
(2021)
Article
Materials Science, Biomaterials
Izabela Stupka, Artur P. Biela, Bernard Piette, Agnieszka Kowalczyk, Karolina Majsterkiewicz, Kinga Borzecka-Solarz, Antonina Naskalska, Jonathan G. Heddle
Summary: Artificial protein cages, such as TRAP-cages, have potential applications in vaccines and drug delivery. TRAP-cages have the ability to control the disassembly conditions by modifying the interface between their building blocks. By using TRAP rings with different numbers of monomers, it is possible to predict the formation of other cages.
JOURNAL OF MATERIALS CHEMISTRY B
(2024)
Article
Materials Science, Biomaterials
Guo Zhang, Yu Wang, Hua Qiu, Lei Lu
Summary: This study presents a one-pot synthesis method for flower-like AMPs@EAMP particles by combining antimicrobial peptides with ellagic acid, offering enlarged surface area, excellent biocompatibility, and broad-spectrum antibacterial activity. In vivo studies indicate their potential for tissue repair and immune barrier reconstruction.
JOURNAL OF MATERIALS CHEMISTRY B
(2024)
Article
Materials Science, Biomaterials
Jiamei Zhang, Lingshuang Wang, Cheng Xu, Yingui Cao, Shengsheng Liu, Rui L. Reis, Subhas C. Kundu, Xiao Yang, Bo Xiao, Lian Duan
Summary: Pluronic F127 modified silk fibroin film with different types of antibacterial agents could accelerate wound recovery by promoting fibroblast adhesion, eradicating bacteria, and facilitating angiogenesis and re-epithelialization.
JOURNAL OF MATERIALS CHEMISTRY B
(2024)
Article
Materials Science, Biomaterials
Yinsheng Liu, Mingyue Wang, Yinfei Hui, Lei Sun, Yanrui Hao, Henlong Ren, Hao Guo, Wu Yang
Summary: In this study, a rare-earth hybrid luminescent material was developed for the detection of a biomarker for anthrax. The material showed excellent selectivity and high sensitivity, allowing for the determination of the biomarker in saliva and urine. Additionally, a convenient point-of-care testing method using fluorescent test paper and a smartphone was established for the initial diagnosis of anthrax.
JOURNAL OF MATERIALS CHEMISTRY B
(2024)
Review
Materials Science, Biomaterials
Wenshuai Yang, Jingsi Chen, Ziqian Zhao, Meng Wu, Lu Gong, Yimei Sun, Charley Huang, Bin Yan, Hongbo Zeng
Summary: Injectable hydrogels with shear-thinning and/or in situ formation properties offer distinct advantages in bioengineering applications, as they can be directly delivered to target sites, possess self-healing abilities, and simplify the implantation process.
JOURNAL OF MATERIALS CHEMISTRY B
(2024)