An iridium oxide nanoparticle and polythionine thin film based platform for sensitive Leishmania DNA detection

An impedimetric label-free genosensor for high sensitive DNA detection is developed. This system is based on a screen-printed carbon electrode modified with the thionine layer and iridium oxide nanoparticles (IrO2 NP). An aminated oligonucleotide probe is immobilized on the IrO2 NP/polythionine modified electrode and ethanolamine was used as a blocking agent. Different diluted PCR amplified DNA samples have been detected. The selectivity and reproducibility of this system are studied and the system was highly reproducible with RSD approximate to 15% and sensitive enough while using 2% of ethanolamine during the blocking step employed for genosensor preparation.