Review
Food Science & Technology
Muhammad Riaz, Ramsha Khalid, Muhammad Afzal, Fozia Anjum, Hina Fatima, Saadiya Zia, Ghulam Rasool, Chukwuebuka Egbuna, Andrew G. Mtewa, Chukwuemelie Zedech Uche, Muhammad Aamir Aslam
Summary: Phytobioactive compounds are plant secondary metabolites with therapeutic potential that can be used as alternative therapeutic agents for various diseases.
FOOD SCIENCE & NUTRITION
(2023)
Review
Agronomy
Rahmatullah Jan, Sajjad Asaf, Muhammad Numan, Lubna, Kyung-Min Kim
Summary: Plant secondary metabolites play crucial roles in plant survival and ecological connections, providing defense against pathogens and stress. The accumulation of these metabolites is highly influenced by environmental factors, and their biosynthesis is regulated by various genes. Transcriptional factors such as WRKY, MYB, AP2/ERF, bZIP, bHLH, and NAC play key roles in regulating secondary metabolite biosynthesis in plants under stress conditions.
Article
Cell Biology
Huriye Ercan, Ulrike Resch, Felicia Hsu, Goran Mitulovic, Andrea Bileck, Christopher Gerner, Jae-Won Yang, Margarethe Geiger, Ingrid Miller, Maria Zellner
Summary: Proteomics is an essential analytical technique for studying biological systems using different proteins. The study compared the qualitative and quantitative performance of two commonly used proteomics techniques, label-free shotgun and 2D-DIGE, using six technical and three biological replicates of the human prostate carcinoma cell line DU145. The results showed that label-free shotgun quickly provides an annotated proteome but with reduced robustness compared to 2D-DIGE, which offers qualitative and quantitative information on proteoforms and post-translational modifications. However, the 2D-DIGE technique requires more time and manual work. Ultimately, this work highlights the different outputs and applications of these two techniques for biological research.
Review
Food Science & Technology
Jinzhu Xiang, Min Du, Hanning Wang
Summary: Dietary supplementation of plant extracts has been shown to enhance skeletal muscle development, reduce muscle atrophy, improve muscle oxidative functions, and has great potential in maintaining muscle mass and improving metabolic diseases.
FOOD REVIEWS INTERNATIONAL
(2023)
Review
Biochemistry & Molecular Biology
Avantika Pandey, Madhoolika Agrawal, Shashi Bhushan Agrawal
Summary: Despite a rich history and economic importance, the potential of medicinal plants has not been fully explored under different abiotic stress conditions. The penetration of UV-B radiation and contamination of heavy metals have a significant impact on the defense-related and pharmaceutically important secondary metabolites of medicinal plants, which can affect both the quality and quantity of these metabolites. Understanding the effects of UV-B and heavy metal contamination on the biosynthesis and accumulation of secondary metabolites is important for the comprehensive study of medicinal plants.
Review
Biochemistry & Molecular Biology
Chattarin Ruchawapol, Man Yuan, Si-Min Wang, Wen-Wei Fu, Hong-Xi Xu
Summary: Herpesviruses can cause various diseases and pose a challenge to global health due to drug resistance. Natural products and their derivatives have shown significant efficacy in treating herpesvirus infections, providing a potential alternative therapy with minimal side effects. Further research and application of these natural compounds are warranted.
Article
Chemistry, Applied
Sridevi Muralidharan, Yan Yee Poon, Graeme C. Wright, Paul A. Haynes, Nanju A. Lee
Summary: This study used mass spectrometry-based proteomic analysis on Australian peanut recombinant inbred lines and found significant protein expression differences between high and low polyphenol expressing RILs. Metabolic changes, including increased enzymatic breakdown of sugars and phenylalanine biosynthesis, were observed in polyphenol-rich RILs. The study also revealed overexpression of phenylpropanoid pathway and antioxidant enzymes, such as catalase, in the polyphenol-rich peanut phenotype.
Review
Biochemical Research Methods
Randolph R. J. Arroo, Avninder S. Bhambra, Christophe Hano, Gulin Renda, Ketan C. Ruparelia, Meng F. Wang
Summary: Analysis of biochemical pathways involves using labelled precursors to monitor metabolic fate, with mass spectrometry and nuclear magnetic resonance as common monitoring tools.
PHYTOCHEMICAL ANALYSIS
(2021)
Review
Plant Sciences
Devina Ghai, Arshpreet Kaur, Parvinderdeep S. Kahlon, Sandip V. Pawar, Jaspreet K. Sembi
Summary: Orchids, with their vast reservoir of secondary metabolites, hold immense therapeutic importance. High-throughput omics technologies have been used to analyze gene identification, functional annotation, and differential expression in orchid transcriptome data sets, with a focus on alkaloid and flavonoid biosynthesis. The study provides insights into potential gene targets for drug discovery and suggests strategies for genetic and epigenetic improvement of orchids for therapeutic product development.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Engineering, Environmental
Sandro Castronovo, Lissa Helmholz, David Wolff, Jan Struckmann Poulsen, Jeppe Lund Nielsen, Thomas A. Ternes, Torsten C. Schmidt, Arne Wick
Summary: The enzymatic processes involved in the degradation of the artificial sweetener acesulfame (ACE) in municipal wastewater treatment have been poorly understood. In this study, two bacterial taxa, an unknown Chelatococcus species and Ensifer adhaerens, were enriched from activated sludge using ACE as the carbon source, and cell-free lysates were analyzed to demonstrate enzymatically catalyzed ACE degradation. The fractionation of the lysate succeeded in separating the enzymes responsible for the initial transformation of ACE and further transformation pathway, providing evidence that the cleavage of the sulfuric ester moiety of ACE is the initial step. The analysis of the unfractionated lysate revealed proteins possibly involved in ACE transformation, including a highly abundant amidase and an ABC transporter substrate-binding protein.
Review
Chemistry, Medicinal
Sergey A. Dyshlovoy
Summary: Natural compounds derived from marine organisms often have unique chemical structures and potent biological activities, including cancer-preventive effects. Recent updates on marine chemopreventive compounds have been summarized, with a particular focus on the molecular mechanisms involved.
Review
Biochemistry & Molecular Biology
Pui Ying Lam, Lanxiang Wang, Clive Lo, Fu-Yuan Zhu
Summary: Plant metabolism plays a crucial role in plant survival, growth, development, and interaction with the environment. It is tightly regulated at transcriptional, post-transcriptional, translational, and post-translational levels in response to different growth stages and changing environment. Alternative splicing, a post-transcriptional regulatory mechanism, generates multiple protein isoforms from a single gene and regulates plant metabolism by altering biochemical activities, protein interactions, and subcellular localization.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Plant Sciences
Andong Yu, Xueqian Jiang, Yan Sun, Qiannan Hu, Xiaoxi Zhu, Junmei Kang, Lin Chen, Lin Liu, Linfeng Hao, Qingchuan Yang, Ruicai Long, Mingna Li
Summary: This study identified 90 UGT genes in alfalfa that are likely related to glycosylation modifications with secondary metabolites. The genes were unevenly distributed and showed specific tissue expression patterns. The study also found that these genes could be induced by phytohormones and flavonoids, indicating their involvement in secondary metabolism.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Agricultural Engineering
Aleya Ferdausi, Xianmin Chang, Meriel Jones
Summary: This study investigated the differential expression of transcripts related to secondary metabolite biosynthesis in Narcissus basal plate and in vitro callus. Enzymes involved in the initial biosynthetic pathways were upregulated in callus, while genes responsible for regulating the later stage of alkaloid biosynthesis were mainly upregulated in field samples. The findings provide insights into the genes and enzymes involved in secondary metabolism in Narcissus plants.
INDUSTRIAL CROPS AND PRODUCTS
(2021)
Article
Green & Sustainable Science & Technology
Virginia Sarropoulou, Eirini Sarrou, Andrea Angeli, Stefan Martens, Eleni Maloupa, Katerina Grigoriadou
Summary: Primula veris subsp. veris is a perennial herbaceous plant whose roots and flowers are valuable pharmaceutical raw materials. An in vitro adventitious root production method was developed to produce bioactive compounds in a controlled environment, leading to higher content compared to soil-grown plants.
Article
Multidisciplinary Sciences
Victoria Florencio-Ortiz, Susana Selles-Marchart, Jose Zubcoff-Vallejo, Georg Jander, Jose L. Casas
Article
Agronomy
Rolord Severe, Laura Morante, Jaime Morante Carriel, Kelly Dumorne
COMMUNICATIONS IN SOIL SCIENCE AND PLANT ANALYSIS
(2019)
Article
Plant Sciences
S. Belchi-Navarro, L. Almagro, R. Bru-Martinez, M. A. Pedreno
PLANT PHYSIOLOGY AND BIOCHEMISTRY
(2019)
Article
Plant Sciences
Victoria Florencio-Ortiz, Susana Selles-Marchart, Jose L. Casas
Summary: Aphid attacks trigger plant defense responses and lead to changes in the proteome of pepper leaves, with low density infestation resulting in a smaller number of differentially expressed proteins compared to high density infestation. The altered proteins are involved in various aspects of plant primary metabolism, with photosynthesis being the most affected process.
Article
Biochemistry & Molecular Biology
Daniela P. Herrera, Andrea M. Chanique, Ascension Martinez-Marquez, Roque Bru-Martinez, Robert Kourist, Loreto P. Parra, Andreas Schuller
Summary: This study reports the engineering of a resveratrol OMT from Vitis vinifera with high catalytic efficiency in producing pterostilbene. Through point mutations and variant design, the native substrate selectivity of VvROMT was successfully altered, indicating its potential for the tailor-made production of stilbenes.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Javier Santamaria-Gomez, Miguel Angel Rubio, Rocio Lopez-Igual, Ana B. Romero-Losada, Fernando M. Delgado-Chaves, Roque Bru-Martinez, Francisco J. Romero-Campero, Antonia Herrero, Michael Ibba, Jesus A. G. Ochoa de Alda, Ignacio Luque
Summary: This study reveals the existence of long tRNA gene arrays in some bacteria, which are induced when the translation machinery is impaired, directly contributing to cell survival. The cellular response to this challenge involves a global reprogramming of the transcriptome in two phases.
NUCLEIC ACIDS RESEARCH
(2021)
Review
Biotechnology & Applied Microbiology
Philippe Jeandet, Eduardo Sobarzo-Sanchez, Md. Sahab Uddin, Roque Bru, Christophe Clement, Cedric Jacquard, Seyed Fazel Nabavi, Maryam Khayatkashani, Gaber El-Saber Batiha, Haroon Khan, Iwona Morkunas, Franscesco Trotta, Adrian Matencio, Seyed Mohammad Nabavi
Summary: Stilbenes, such as resveratrol and its derivatives, have interesting properties but limited by their poor aqueous solubility and low bioavailability. Cyclodextrins play a significant role in stilbene chemistry, forming nanomolecular inclusion complexes for drug delivery and synthesis of derivatives. Plant cell systems offer a feasible way for large-scale production of stilbenes, requiring elicitor compounds like cyclodextrins for biosynthesis induction.
BIOTECHNOLOGY ADVANCES
(2021)
Article
Biochemistry & Molecular Biology
Elias Hurtado-Gaitan, Susana Selles-Marchart, James Hartwell, Maria Jose Martinez-Esteso, Roque Bru-Martinez
Summary: The study found that under CD induction conditions, PPCK was down-regulated, stilbene production increased, and PPC activity was lost in both cell cultures and leaves. Additionally, STS transcripts were co-induced with MYB14 and WRKY24 in both tissues. These genes had not been previously reported to respond to CD in grape leaves.
Article
Biochemistry & Molecular Biology
Juan Carlos Vera-Urbina, Susana Selles-Marchart, Ascension Martinez-Marquez, Maria Jose Martinez-Esteso, Maria Angeles Pedreno, Jaime Morante-Carriel, Roque Bru-Martinez
Summary: This study presents the characterization and optimization of t-R production in grape cell cultures. Factors such as growth phase, elicitor concentration, biomass, order of addition, and cell conditions were investigated. The optimal conditions for t-R production were determined, and the results can guide the upscaling of the bioproduction system.
Article
Pharmacology & Pharmacy
Wiston Javier Morales-Rodriguez, Mercedes Susana Carranza-Patino, Jaime Alfredo Morante-Carriel, Angie Briggette Castro Maruri
Summary: This study evaluated the use of pectintranseliminase (PTE) to mitigate cadmium concentration in cocoa beans through a fast fermentation process. The results showed that the application of PTE significantly reduced the cadmium content in cocoa beans, leading to improved physical and sensory quality of the beans.
JOURNAL OF PHARMACEUTICAL NEGATIVE RESULTS
(2022)
Article
Pharmacology & Pharmacy
Mercedes Susana Carranza Patino, Sherly Liliam Roca Moreno, Jaime Alfredo Morante Carriel, Rolando Manuel Lopez Tobar
Summary: The objective of this research was to evaluate the vegetative development of cuttings of tropical Eucalyptus clones with auxin application. The results showed that clone EC166 had better response to 1500 mg kg(-1) AIB, while the number of roots showed better response to 1000 mg kg(-1) ANA.
JOURNAL OF PHARMACEUTICAL NEGATIVE RESULTS
(2022)
Review
Biochemistry & Molecular Biology
Philippe Jeandet, Alessandro Vannozzi, Eduardo Sobarzo-Sanchez, Md. Sahab Uddin, Roque Bru, Ascension Martinez-Marquez, Christophe Clement, Sylvain Cordelier, Azadeh Manayi, Seyed Fazel Nabavi, Mahsa Rasekhian, Gaber El-Saber Batiha, Haroon Khan, Iwona Morkunas, Tarun Belwal, Jingjie Jiang, Mattheos Koffas, Seyed Mohammad Nabavi
Summary: Phytostilbenes, despite being a limited chemical family, represent a significant group of molecules among natural compounds. Research interest in these compounds has continuously grown, leading to a vast literature development and tens of thousands of references. The various aspects of phytostilbenes, including their chemistry, biosynthesis regulation, biological activities, molecular engineering, and biotechnological production, have been extensively covered in over 500 articles.
NATURAL PRODUCT REPORTS
(2021)
Article
Agronomy
Ramon Jaimez, Gregorio Vasconez, Ignacio Sotomayor, Grisnel Quijano, Jaime Morante, Francisco Arteaga, George Cedeno-Garcia
Summary: Grafted cacao seedlings showed differences in water potential, basal diameter, chlorophyll content, and leaf nitrogen concentration under water deficit conditions, with the combination of EET400 rootstock and scion demonstrating better adaptability.
REVISTA DE LA FACULTAD DE AGRONOMIA DE LA UNIVERSIDAD DEL ZULIA
(2021)
Article
Biotechnology & Applied Microbiology
Peter Vera, Marcia Astorga-Elo, Rolord Severe, Laura Morante, Jaime Morante, Jhon Montenegro, Kelly Dumorne
ROMANIAN BIOTECHNOLOGICAL LETTERS
(2020)