Article
Microbiology
Gebremeskel Mamu Werid, Yassein M. Ibrahim, Hongyan Chen, Lizhi Fu, Yue Wang
Summary: This study conducted an epidemiological investigation and sequence analysis to explore the distribution and genetic diversity of porcine astrovirus (PAstV), porcine encephalomyocarditis virus (EMCV), porcine kobuvirus (PKV), and porcine sapovirus (PSaV) in healthy pigs. The results showed that PKV was the most prevalent virus, followed by PAstV, PSaV, and EMCV. Furthermore, coinfection of multiple viruses was found in healthy pigs.
Article
Food Science & Technology
Catherine Hennechart-Collette, Oceane Dehan, Michel Laurentie, Audrey Fraisse, Sandra Martin-Latil, Sylvie Perelle
Summary: The research characterized a method derived from ISO standard to detect HAV, HEV and norovirus in artificially-contaminated multicomponent foodstuffs. Results showed no significant difference in recovery rates among settings and extraction yields varied for different viruses. LOD95 and LOQ values were determined for different types of viruses.
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2021)
Article
Food Science & Technology
Catherine Hennechart-Collette, Oceane Dehan, Michel Laurentie, Audrey Fraisse, Sandra Martin-Latil, Sylvie Perelle
Summary: Viruses, including human norovirus and hepatitis viruses, are a major cause of foodborne disease worldwide. This study validated a new membrane material and method for detecting enteric viruses in tap and bottled water. The results showed that the virus recovery rates varied depending on the water samples tested.
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2022)
Article
Agriculture, Dairy & Animal Science
Julia Lienhard, Isabelle Vonlanthen-Specker, Xaver Sidler, Claudia Bachofen
Summary: Hepatitis E virus is a significant cause of acute hepatitis worldwide, mainly originating from animals, with pigs being the primary reservoir. The study aimed to establish a screening tool for detecting HEV in pig farms and found that sock swabs were useful for screening, with a high prevalence of HEV in Swiss pig herds and the presence of the same viral subtype in both pig farms and human patients.
Article
Environmental Sciences
Juan Martin Oteiza, Veronica Emilse Prez, Dayana Pereyra, Maria Virginia Jaureguiberry, Gloria Sanchez, Anderson S. Sant'Ana, Patricia Angelica Barril
Summary: Berries have been implicated in the transmission of norovirus and hepatitis A virus outbreaks. This study investigated the virological quality of berries cultivated in Argentina and detected the presence of norovirus GII.6 in one out of 184 samples. The data collected provides important reference information for assessing the potential risk of soft fruits as vehicles for foodborne pathogenic viruses.
FOOD AND ENVIRONMENTAL VIROLOGY
(2022)
Article
Immunology
Herman M. Chambaro, Michihito Sasaki, Walter Muleya, Masahiro Kajihara, Misheck Shawa, Kabemba E. Mwape, Hayato Harima, Yongjin Qiu, William W. Hall, Paul Fandamu, David Squarre, Edgar Simulundu, Hirofumi Sawa, Yasuko Orba
Summary: The study revealed a seroprevalence rate of 47.7% for HEV antibodies in domestic pigs in Zambia, and 16.0% of slaughtered pigs were found to have zoonotic genotype 3 HEV RNA. This is the first report of HEV infection in domestic pigs in Zambia, highlighting a potential risk of contamination in the pork supply chain and zoonotic transmission to abattoir workers, pig farmers, and handlers.
EMERGING MICROBES & INFECTIONS
(2021)
Article
Veterinary Sciences
Baoyuan Liu, Yiyang Chen, Meimei Zhang, Tianxiang Chen, Yuan Zhang, DanBaZha Xi, Shixuan Xu, Qin Zhao, En-Min Zhou
Summary: This study confirms the presence of HEV infection in laboratory Bama miniature pigs in China and demonstrates that these pigs can be experimentally infected with HEV. Screening of laboratory pigs for HEV is important to prevent zoonotic infections.
BMC VETERINARY RESEARCH
(2022)
Article
Veterinary Sciences
Giovanni Ianiro, Enrico Pavoni, Giuseppe Aprea, Romina Romantini, Giovanni Loris Alborali, Daniela D'Angelantonio, Giuliano Garofolo, Silvia Scattolini, Luca De Sabato, Chiara Francesca Magistrali, Elke Burow, Fabio Ostanello, Richard Piers Smith, Ilaria Di Bartolo
Summary: Foodborne transmission is considered the main way of spreading zoonotic hepatitis E virus (HEV) infection in Europe. Pigs are recognized as the main reservoir of the zoonotic HEV-3 genotype, and pork products are often indicated as the source of many human foodborne HEV cases. The prevalence of HEV-3 in pig herds across EU confirms the widespread circulation of this genotype.
FRONTIERS IN VETERINARY SCIENCE
(2023)
Article
Veterinary Sciences
Anna Jackova, Katarina Dudasova, Slavomira Salamunova, Rene Mandelik, Jaroslav Novotny, Stefan Vilcek
Summary: This study investigated the circulation of HEV among domestic swine in Slovakia and the genetic diversity of the virus. The results showed a lower prevalence of HEV in Slovak pig farms compared to other European countries, with most isolates belonging to HEV-3 clade 3abchij but with three sequences remaining unclassified.
BMC VETERINARY RESEARCH
(2021)
Article
Veterinary Sciences
Gebremeskel Mamu Werid, He Zhang, Yassein M. Ibrahim, Yu Pan, Lin Zhang, Yunfei Xu, Wenli Zhang, Wei Wang, Hongyan Chen, Lizhi Fu, Yue Wang
Summary: A multiplex RT-PCR assay was developed to simultaneously detect multiple viruses in swine fecal samples. The assay is simple, rapid, and cost-effective, and showed good performance when applied to field samples.
VETERINARY SCIENCES
(2022)
Article
Public, Environmental & Occupational Health
Maria Casares-Jimenez, Pedro Lopez-Lopez, Javier Caballero-Gomez, Mario Frias, Belen Perez-Hernando, Adeolu Sunday Oluremi, Maria A. Risalde, Inmaculada Ruiz-Caceres, Oluyinka Oladele Opaleye, Ignacio Garcia-Bocanegra, Antonio Rivero-Juarez, Antonio Rivero
Summary: Our study aimed to describe and characterize the global distribution of Hepatitis E virus (HEV) genotypes in domestic pig and wild boar. Most sequences were categorized as genotype 3, with genotype 4 being widely distributed in Asia. There is a need for increased sequencing of swine HEV to improve understanding and surveillance efforts.
Editorial Material
Biology
Altaira D. Dearborn, Ashish Kumar, Joseph Marcotrigiano
Summary: A domain in the ORF1 polyprotein of the hepatitis E virus, previously believed to be a protease, is actually a zinc-binding domain.
Article
Public, Environmental & Occupational Health
Vanessa Mendonca Soares, Emanoelli Aparecida Rodrigues dos Santos, Leonardo Ereno Tadielo, Camila Koutsodontis Cerqueira-Cezar, Aryele Nunes da Cruz Encide Sampaio, Ana Karolina Antunes Eisen, Kelen Gras de Oliveira, Matheus Beltrame Padilha, Maria Eduarda de Moraes Guerra, Raissa Gasparetto, Mario Celso Sperotto Brum, Carolina Kist Traesel, Andreia Henzel, Fernando Rosado Spilki, Juliano Goncalves Pereira
Summary: This study investigated the presence of adenovirus, rotavirus, and hepatitis E virus in beef, pork, and chicken meat cuts in a Brazilian city. The results showed that chicken meat had a higher frequency of adenovirus and rotavirus isolates, and supermarkets had a higher frequency of rotavirus compared to other commercial establishments. Adenovirus and rotavirus were simultaneously identified in a small number of samples from supermarkets and grocery stores.
Article
Immunology
Claire P. Mattison, Molly Dunn, Mary E. Wikswo, Anita Kambhampati, Laura Calderwood, Neha Balachandran, Eleanor Burnett, Aron J. Hall
Summary: Between 2009 and 2018, numerous gastroenteritis outbreaks were reported to the US National Outbreak Reporting System, with the majority attributed to person-to-person transmission in long-term care facilities, daycares, and schools. Investigations of norovirus-negative outbreaks should include testing for adenovirus, astrovirus, rotavirus, and sapovirus.
EMERGING INFECTIOUS DISEASES
(2021)
Article
Food Science & Technology
Catherine Hennechart-Collette, Lisa Fourniol, Audrey Fraisse, Sandra Martin-Latil, Sylvie Perelle
Summary: This study aimed to characterize a proteinase K method for the detection of human norovirus, hepatitis A virus, and hepatitis E virus in artificially contaminated dairy products. The method proved to be suitable for routine diagnostic needs in dairy products.
Article
Microbiology
Peter H. Otte, Stefanie Rosenhain, Mandy C. Elschner, Helmut Hotzel, Patrycja Machnowska, Eva Trojnar, Kathrin Hoffmann, Reimar Johne
VETERINARY MICROBIOLOGY
(2015)
Article
Multidisciplinary Sciences
Patrycja Machnowska, Andrea Hauser, Karolin Meixenberger, Britta Altmann, Norbert Bannert, Eva Rempis, Alexandra Schnack, Sarah Decker, Vera Braun, Priscilla Busingye, John Rubaihayo, Gundel Harms, Stefanie Theuring
Article
Biochemical Research Methods
Andrea Hauser, Karolin Meixenberger, Patrycja Machnowska, Stefan Fiedler, Kirsten Hanke, Alexandra Hofmann, Barbara Bartmeyer, Viviane Bremer, Norbert Bannert, Claudia Kuecherer
JOURNAL OF VIROLOGICAL METHODS
(2018)
Article
Biotechnology & Applied Microbiology
Jana Sachsenroeder, Anne Braun, Patrycja Machnowska, Terry Fei Fan Ng, Xutao Deng, Sebastian Guenther, Samuel Bernstein, Rainer G. Ulrich, Eric Delwart, Reimar Johne
JOURNAL OF GENERAL VIROLOGY
(2014)
Article
Gastroenterology & Hepatology
R. Johne, J. Reetz, R. G. Ulrich, P. Machnowska, J. Sachsenroeder, P. Nickel, J. Hofmann
JOURNAL OF VIRAL HEPATITIS
(2014)
Article
Microbiology
Peter H. Otto, Muzahed Uddin Ahmed, Helmut Hotzel, Patrycja Machnowska, Jochen Reetz, Bernhard Roth, Eva Trojnar, Reimar Johne
VETERINARY MICROBIOLOGY
(2012)
Article
Multidisciplinary Sciences
Patrycja Machnowska, Karolin Meixenberger, Daniel Schmidt, Heiko Jessen, Heribert Hillenbrand, Barbara Gunsenheimer-Bartmeyer, Osamah Hamouda, Claudia Kuecherer, Norbert Bannert, H. Knechten, P. Panstruga, K. Arasteh, M. Rittweger, M. Schuermann, H. Wesselmann, A. Moll, U. Bohr, A. B. Jessen, H. Schulbin, W. Schmidt, H. Karcher, K. Fischer, D. Schranz, M. Vallee, J. A. Claus, C. Thomas, R. Grimm, G. Klausen, C. Cordes, N. H. Brockmeyer, A. Potthoff, S. Roessler, M. Hower, C. Bachmann, P. Spornraft-Ragaller, D. Teichmann, D. Haeussinger, B. Jensen, P. A. Arbter, U. Haars, S. Esser, P. Schenk-Westkamp, S. Usadel, M. C. Mueller, B. Kreft, D. Lange, S. Polywka, O. Degen, A. Plettenbger, F. Kuhlendahl, D. Wiemer, R. E. Schmidt, G. Behrens, M. W. Pletz, B. T. Schleenvoigt, A. Rieke, S. M. Schneeweiss, S. H. A. Scholten, T. Gruenewald, S. Weidemann, B. Claus, P. R. Galle, M. Sprinzl, M. P. Ebert, R. Vogelmann, J. Roider, J. Bogner, A. Todorova, C. Traidl-Hoffmann, H. Jaeger, B. Mueck, R. Pauli, C. D. Spinner, J. Schneider, N. Postel, M. Anzboeck, R. Baumann, N. Schuebel, C. Berning, F. Audebert, A. Trein, E. Schnaitmann, A. Ulmer, M. Mueller, G. Haerter, B. Gruener
Article
Virology
Bo Wang, Luise Krueger, Patrycja Machnowska, Amare Eshetu, Barbara Gunsenheimer-Bartmeyer, Viviane Bremer, Andrea Hauser, Norbert Bannert, C. -Thomas Bock
Article
Microbiology
Folakemi Abiodun Osundare, Patrycja Klink, Catharina Majer, Olusola Aanuoluwapo Akanbi, Bo Wang, Mirko Faber, Dominik Harms, C-Thomas Bock, Oladele Oluyinka Opaleye
Article
Multidisciplinary Sciences
Fisayo Temilade Osamudiamen, Olusola Aanuoluwapo Akanbi, Daniel Oladimeji Oluwayelu, C. Thomas Bock, Patrycja Klink
Summary: This study is the first to detect the presence of aHEV antibodies in commercial layer chickens in Nigeria, with a certain infection rate found. The detection of anti-aHEV antibodies in commercial layer chickens emphasizes the need for disease monitoring in the region, and further studies are essential to assess the real burden of aHEV.
Article
Virology
Fisayo Temilade Osamudiamen, Olusola Aanuoluwapo Akanbi, Steffen Zander, Daniel Oladimeji Oluwayelu, Claus-Thomas Bock, Patrycja Klink
Summary: The study revealed the presence of aHEV in apparently healthy layer chickens in southwestern Nigeria, with the identification of a new genotype. This is the first report of co-circulation of aHEV genotypes in chickens in Nigeria, providing insights into the prevalence and characteristics of aHEV in Nigerian poultry.
Article
Virology
Olufisayo Adeyemi Adesina, Olusola Anuoluwapo Akanbi, Oluyinka Oladele Opaleye, Margaret Oluwatoyin Japhet, Bo Wang, Adekemi Olubukunola Oluyege, Patrycja Klink, C-Thomas Bock
Summary: A study in Nigeria revealed the presence of immune escape mutations (IEMs) in hepatitis B virus (HBV) isolates, including the Q129H mutation, but no drug resistance mutations were observed. The research underscores the importance of intensified studies on the dynamics of IEMs to enhance the elimination of HBV.
Article
Veterinary Sciences
Susanne Kreuzer, Patrycja Machnowska, Jens Assmus, Matthias Sieber, Robert Pieper, Michael F. G. Schmidt, Gudrun A. Brockmann, Lydia Scharek-Tedin, Reimar Johne
VETERINARY RESEARCH
(2012)