Article
Chemistry, Multidisciplinary
Sarah H. Klass, Laura E. Sofen, Zachary F. Hallberg, Tahoe A. Fiala, Alexandra Ramsey, Nicholas S. Dolan, Matthew B. Francis, Ariel L. Furst
Summary: Pathogenic E. coli strains are a significant threat to public health, causing both foodborne illnesses and urinary tract infections. By using a rapid bioconjugation reaction and electrochemical impedance spectroscopy, we can selectively capture and accurately quantify these pathogenic microbes on a disposable gold electrode.
CHEMICAL COMMUNICATIONS
(2021)
Article
Microbiology
Danielle M. Tack, Hannah M. Kisselburgh, LaTonia C. Richardson, Aimee Geissler, Patricia M. Griffin, Daniel C. Payne, Brigette L. Gleason
Summary: The study summarizes the epidemiology and transmission routes of STEC outbreaks in the United States from 2010 to 2017, finding that foodborne transmission is the most common route, and there are differences between O157 and non-O157 outbreaks in terms of food sources, demographic characteristics, and severity.
Article
Food Science & Technology
Joseph A. Capobianco, Cheryl M. Armstrong, Joe Lee, Andrew G. Gehring
Summary: A novel flowthrough immunoelectrochemical biosensor was developed for rapid detection of pathogenic bacteria in food, allowing for high volumes of samples to be analyzed without affecting the limit of detection.
Article
Food Science & Technology
Shuhong Zhang, Yuanbin Huang, Moutong Chen, Guangzhu Yang, Jumei Zhang, Qingping Wu, Juan Wang, Yu Ding, Qinghua Ye, Tao Lei, Yue Su, Rui Pang, Runshi Yang, Youxiong Zhang
Summary: This study systematically characterized E. coli O157:non-H7 strains isolated from retail food in Chinese markets. The strains displayed diverse H serotypes, virulence genes, and antibiotic resistance. Some strains also harbored plasmid-mediated colistin-resistant genes, which is a significant concern.
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2022)
Article
Infectious Diseases
Ana Carolina Abrantes, Sergio Santos-Silva, Joao Mesquita, Madalena Vieira-Pinto
Summary: This study aims to determine the occurrence of Hepatitis E virus (HEV) in the wild boar population in Portugal. Analysis of collected samples during the hunting season revealed a low prevalence of HEV in the liver and faeces, but a higher seropositivity rate. A hotspot of HEV was found in the southeast of central Portugal, posing a zoonotic risk for hunters in the region.
TROPICAL MEDICINE AND INFECTIOUS DISEASE
(2023)
Review
Food Science & Technology
Songeeta Singha, Rajendran Thomas, Jai Narain Viswakarma, Vivek Kumar Gupta
Summary: Foodborne illnesses are a major cause of morbidity and mortality worldwide. Escherichia coli O157 is a commonly reported pathogen associated with foodborne outbreaks. The South East Asia region has the highest burden of diarrhoeal mortality, particularly among children. This article focuses on the distribution of E. coli O157 in the food chains of South East Asian countries, with a special emphasis on India where a significant number of child diarrhoeal deaths occur each year. The article also discusses detection methods and control measures for E. coli O157, aiming to document and highlight the extent of foodborne infections and promote effective preventive measures.
JOURNAL OF FOOD SCIENCE AND TECHNOLOGY-MYSORE
(2023)
Article
Biochemistry & Molecular Biology
Yuqing Zhou, Ziyong Li, Jijie Huang, Yuxing Wu, Xinyu Mao, Yizhou Tan, Hui Liu, Dongxin Ma, Xun Li, Xiaoye Wang
Summary: In this study, an electrochemical biosensor using phage EP01 as the recognition agent for detection of E. coli O157:H7 GXEC-N07 was established. The biosensor showed good linearity in the concentration range of 10(2) CFU/mL to 10(2) CFU/mL with a low detection limit of 11.8 CFU/mL, and the whole process was completed in less than 30 min. The biosensor was successfully applied to the quantitative detection of GXEC-N07 in fresh milk and raw pork. The recovery values ranged from 60.8% to 114.2%. The biosensor provides a rapid, specific, low cost, and label free tool for E. coli O157:H7 GXEC-N07 detection, and is expected to become a powerful method for the detection of bacteria threatening food safety and public health security.
BIOELECTROCHEMISTRY
(2023)
Article
Microbiology
Yan Zhou, Qiyang Wan, Hongduo Bao, Yonghao Guo, Shujiao Zhu, Hui Zhang, Maoda Pang, Ran Wang
Summary: Phage SQ17 is a broad-host range lytic phage capable of infecting various E. coli strains, with a short latent period, high burst size, and stability. Experimental results demonstrate that SQ17 can effectively control the counts of EHEC O157:H7 and ETEC in milk, raw beef, and fresh lettuce.
FRONTIERS IN MICROBIOLOGY
(2022)
Review
Food Science & Technology
Osman Y. Y. Koyun, Igori Balta, Nicolae Corcionivoschi, Todd R. R. Callaway
Summary: North America is a major beef producer, with about 12% of the world's cattle inventory. Feedlots play a crucial role in modern cattle production by providing high-quality protein food for humans. Cattle in feedlots are vulnerable to certain zoonotic diseases that affect their health, growth, and meat quality, as well as human health. These diseases can be transmitted among pen-mates or through the environment, vectors, or fomites. Pathogens carried by cattle in their gastrointestinal tract can contaminate food and the feedlot environment, leading to the prolonged circulation of fecal-oral transmission pathogens within the cattle population. Diseases such as Salmonella, Shiga toxin-producing Escherichia coli, and Campylobacter, commonly associated with animal-derived foods, can be transmitted to humans through contact with infected cattle or consumption of contaminated meat. Neglected zoonotic diseases like Brucellosis, anthrax, and leptospirosis, have significant impacts on both human and animal health.
Article
Food Science & Technology
Chuyan Chen, Claudia P. Coronel-Aguilera, Bruce M. Applegate, Andrew G. Gehring, Arun K. Bhunia, George C. Paoli
Summary: The USDA-FSIS has zero tolerance for E. coli O157:H7 in raw ground beef and currently collects samples from beef processing facilities for testing. This study assessed the potential of using a phage-based luminescence detection assay during sample transportation, and the results suggest that this approach is feasible.
Article
Chemistry, Analytical
Shayesteh Bazsefidpar, Esther Serrano-Pertierra, Gemma Gutierrez, Alberto Sanchez Calvo, Maria Matos, Maria Carmen Blanco-Lopez
Summary: The study developed a sensitive lateral flow immunoassay (LFIA) for rapid detection of Escherichia coli (E. coli) O157:H7, a pathogen responsible for worldwide diseases and fatalities. Gold nanoparticles were synthesized and coated with a carboxylated heterobifunctional PEG polymer to be used as a label in sandwich-LFIA. A silver enhancement strategy was developed to amplify the signal, reducing the limit of detection (LOD). The system demonstrated improved sensitivity compared to the conventional LFIA system and showed effectiveness in quickly detecting pathogenic bacteria in food products.
Review
Veterinary Sciences
Kinza Javed, Khalid A. Alkheraije
Summary: Globally, foodborne illnesses are a major concern for morbidity and high rates of death in the human community. Cryptosporidium, a significant foodborne zoonotic parasite, is a common cause of diarrhea worldwide. The main disease-causing agents, Cryptosporidium hominis and Cryptosporidium parvum, have been identified as capable of causing moderate to severe illness in humans. The zoonotic reservoirs for Cryptosporidium are domestic animals, such as cattle and humans. Disease transmission occurs through ingestion of oocyst from animals or humans, fecal-oral transmission, as well as consumption of tainted water and food. Infected food materials, including lettuce, spinach, tomato, and strawberries, show varying levels of Cryptosporidium prevalence. Nitazoxanide is currently the only authorized medication for treating cryptosporidiosis, although other medications have been used due to their clinical effectiveness. The severity of Cryptosporidium outbreaks in humans ranges from 0.9% to 39.6%. This review highlights the importance of foodborne zoonosis in humans and farm animals, discussing the transmission rate and presence of Cryptosporidium from different sources in food materials.
PAKISTAN VETERINARY JOURNAL
(2023)
Article
Food Science & Technology
Man-Seok Choi, Eun Bi Jeon, Ji Yoon Kim, Eun Ha Choi, Jun Sup Lim, Jinsung Choi, Shin Young Park
Summary: The efficacy of dielectric barrier discharge (DBD) plasma treatment for reducing non-pathogenic Escherichia coli and E. coli O157:H7 in oysters was studied. Exposure to DBD plasma for at least 60 minutes resulted in significant reductions in both types of bacteria, with minimal impact on the glycogen content and texture of the oysters. This non-thermal intervention could be a promising method for improving the safety of fresh oysters.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2022)
Article
Food Science & Technology
Jin Hong Mok, Ye Niu, Ahmed Yousef, Yi Zhao, Sudhir K. Sastry
Summary: This study investigated the persistence of Escherichia coli O157:H7 on leafy greens and how fluid shear affects bacterial colonization on these surfaces. Results showed that microcolony distribution was influenced by shear flow, suggesting the need for design of irrigation and washing systems to mitigate contamination of leafy greens.
INNOVATIVE FOOD SCIENCE & EMERGING TECHNOLOGIES
(2022)
Article
Food Science & Technology
Patrizio Lorusso, Elisabetta Bonerba, Annamaria Pandiscia, Anna Mottola, Angela Di Pinto, Roberta Piredda, Valentina Terio
Summary: This study aimed to assess the occurrence and genetic variability of HEV in wild boars. It found a prevalence of 26.7% in wild boar liver samples, all identified as HEV-3c subtype. Further research is needed to investigate virus viability and its link to human seroprevalence.
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2022)
Article
Veterinary Sciences
Anthony W. Confer, Timothy A. Snider, Jared D. Taylor, Marie Montelongo, Nicholas J. Sorensen
AMERICAN JOURNAL OF VETERINARY RESEARCH
(2016)
Article
Agriculture, Dairy & Animal Science
Jared D. Taylor, Merel Rodenburg, Timothy A. Snider
JOURNAL OF DAIRY SCIENCE
(2017)
Article
Geriatrics & Gerontology
Warren Ladiges, Jessica M. Snyder, Erby Wilkinson, Denise M. Imai, Tim Snider, Xuan Ge, Marcia Ciol, Christina Pettan-Brewer, Smitha P. S. Pillai, John Morton, Ellen Quarles, Peter Rabinovitch, Laura Niedernhofer, Denny Liggitt
JOURNALS OF GERONTOLOGY SERIES A-BIOLOGICAL SCIENCES AND MEDICAL SCIENCES
(2017)
Article
Immunology
Jennifer H. Shaw, Amanda R. Behar, Timothy A. Snider, Noah A. Allen, Erika I. Lutter
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2017)
Article
Pathology
Harshini K. Ashar, Nathan C. Mueller, Jennifer M. Rudd, Timothy A. Snider, Mallika Achanta, Maram Prasanthi, Sivasami Pulavendran, Paul G. Thomas, Akhilesh Ramachandran, Jerry R. Malayer, Jerry W. Ritchey, Rachakatla Rajasekhar, Vincent T. K. Chow, Charles T. Esmon, Narasaraju Teluguakula
AMERICAN JOURNAL OF PATHOLOGY
(2018)
Article
Geriatrics & Gerontology
Timothy A. Snider, Arlan Richardson, Julie A. Stoner, Sathyaseelan S. Deepa
Article
Biochemical Research Methods
Alexander Lim, Bryan Naidenova, Haley Bates, Karyn Willyerd, Timothy Snider, Matthew Brian Couger, Charles Chen, Akhilesh Ramachandran
JOURNAL OF MICROBIOLOGICAL METHODS
(2019)
Article
Immunology
Jennifer M. Rudd, Sivasami Pulavendran, Harshini K. Ashar, Jerry W. Ritchey, Timothy A. Snider, Jerry R. Malayer, Montelongo Marie, Vincent T. K. Chow, Teluguakula Narasaraju
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2019)
Article
Geriatrics & Gerontology
Jessica M. Snyder, Timothy A. Snider, Marcia A. Ciol, John E. Wilkinson, Denise M. Imai, Kerriann M. Casey, Jose G. Vilches-Moure, Christina Pettan-Brewer, Smitha P. S. Pillai, Sebastian E. Carrasco, Shabnam Salimi, Warren Ladiges
Article
Microbiology
Sahlu Ayalew, Betsy K. Murdock, Timothy A. Snider, Anthony W. Confer
VETERINARY MICROBIOLOGY
(2019)
Article
Immunology
Sivasami Pulavendran, Maram Prasanthi, Akhilesh Ramachandran, Rezabek Grant, Timothy A. Snider, Vincent T. K. Chow, Jerry R. Malayer, Narasaraju Teluguakula
FRONTIERS IN IMMUNOLOGY
(2020)
Article
Pathology
Harshini K. Ashar, Sivasami Pulavendran, Jennifer M. Rudd, Prasanthi Maram, Mallika Achanta, Vincent T. K. Chow, Jerry R. Malayer, Timothy A. Snider, Narasaraju Teluguakula
Summary: Excessive neutrophil influx, NETs release, and extracellular histones are associated with disease severity in influenza-infected patients. Targeting the neutrophil chemokine receptor CXCR2 could attenuate neutrophilic inflammation and improve outcomes in influenza infections. Timing of treatment with CXCR2 antagonist may play a critical role in mitigating lung pathology during influenza.
AMERICAN JOURNAL OF PATHOLOGY
(2021)
Article
Multidisciplinary Sciences
Zhou Jiang, Juan Wang, Denise Imai, Tim Snider, Jenna Klug, Ruby Mangalindan, John Morton, Lida Zhu, Adam B. Salmon, Jackson Wezeman, Jiayi Hu, Vinal Menon, Nicholas Marka, Laura Neidernhofer, Warren Ladiges
Summary: Pharmaceutical intervention of aging requires targeting multiple pathways, and combining drugs shown to extend lifespan and healthy aging in mice can delay aging phenotypes more effectively in middle-aged mice than any individual drug.
SCIENTIFIC REPORTS
(2022)
Article
Pathology
John Morton, Timothy A. Snider
PATHOBIOLOGY OF AGING AND AGE-RELATED DISEASES
(2017)
Article
Medicine, Research & Experimental
Lana S. Rothenburg, Timothy A. Snider, Allison Wilson, Anthony W. Confer, Akhilesh Ramachandran, Rinosh Mani, Theresa Rizzi, Laura Nafe
MEDICAL MYCOLOGY CASE REPORTS
(2017)