Article
Immunology
Stelli G. Stancheva, Janna Frombling, Elena L. Sassu, Isabel Hennig-Pauka, Andrea Ladinig, Wilhelm Gerner, Tom Grunert, Monika Ehling-Schulz
Summary: This study provides an in-depth analysis of the exoproteome of Actinobacillus pleuropneumoniae serotype 2, revealing potential therapeutic and vaccine targets and highlighting differences in the immune response to different Apx toxins.
MICROBIAL PATHOGENESIS
(2022)
Article
Agronomy
Peter Hoelzen, Tobias Warnck, Steffen Hoy, Kathleen Schlegel, Isabel Hennig-Pauka, Horst Gaumann
Summary: This study compared two licensed A. pleuropneumoniae vaccines in pigs, with results showing that the vaccine containing ApxI-III toxoids was more effective in preventing pulmonary lesions associated with A. pleuropneumoniae infection. Mortality and antimicrobial medication were significantly lower in the vaccine group compared to the control group.
Article
Microbiology
Wei Peng, Xia Yang, Kang Yan, Huanchun Chen, Fangyan Yuan, Weicheng Bei
Summary: Copper is required for many physiological and pathological processes in organisms, and the CopA gene plays a crucial role in providing resistance to copper in A. pleuropneumoniae, protecting against copper toxicity through copper efflux.
VETERINARY MICROBIOLOGY
(2021)
Review
Veterinary Sciences
Maria M. Soto Perezchica, Alma L. Guerrero Barrera, Francisco J. Avelar Gonzalez, Teodulo Quezada Tristan, Osvaldo Macias Marin
Summary: Actinobacillus pleuropneumoniae (App) is a globally distributed Gram-negative bacterium that causes porcine pleuropneumonia. Despite the high morbidity and mortality rates associated with this highly contagious disease in the swine industry, there is currently no effective vaccine available. The recent findings on surface structures and proteins of the bacterium highlight their potential as immunogens for vaccine development.
FRONTIERS IN VETERINARY SCIENCE
(2023)
Article
Veterinary Sciences
Kamonpun Chuekwon, Chun-Yen Chu, Li-Ting Cheng
Summary: The study shows that the N-terminus of flagellin (nFliC) is a versatile and potent adjuvant for vaccine formulation. Both genetic fusion and simple admixture of nFliC with the antigen ApxIIPF enhance humoral and cellular immune responses, and increase vaccine protective efficacy. Even without supplemental adjuvants, genetically linked nFliC still provides significant immune enhancement.
BMC VETERINARY RESEARCH
(2022)
Article
Microbiology
Judith Guitart-Matas, Narjol Gonzalez-Escalona, Meghan Maguire, Anna Vilaro, Jaime Martinez-Urtaza, Lorenzo Fraile, Lourdes Migura-Garcia
Summary: This study provides a comprehensive analysis of the genetic variability within Actinobacillus pleuropneumoniae (APP) serotypes and enhances the understanding of transmission and epidemiological patterns. The correlation between resistance and toxin profiles with the presence of specific genetic elements and serotypes is also determined.
MICROBIOLOGY SPECTRUM
(2022)
Article
Veterinary Sciences
Hugo Dunlop, Steven McOrist
Summary: This study investigated diagnostic serology for Actinobacillus pleuropneumoniae (APP) infections in naturally infected and vaccinated pigs. Various APP serotypes were detected using ELISAs, and the efficacy of vaccines in preventing specific serotype infections was observed.
JOURNAL OF SWINE HEALTH AND PRODUCTION
(2021)
Article
Genetics & Heredity
Li Zhang, Fan Zhao, Huan Xu, Yubing Chen, Chao Qi, Jinlin Liu
Summary: In this study, the researchers investigated the biological role of HtrA in A. pleuropneumoniae infection. The results showed that HtrA plays a significant role in the survival and growth of A. pleuropneumoniae during stressful conditions, affecting stress tolerance, oxidative stress response, in vivo colonization ability, and virulence.
Article
Pathology
Francesca Soutter, Simon L. Priestnall, Brian Catchpole, Andrew N. Rycroft
Summary: This study evaluated an in-vivo model as a potential replacement for existing respiratory infection models. Different strains of Actinobacillus pleuropneumoniae (App) and Apx toxins were injected into the abdominal skin of pigs. High concentrations of certain App strains caused visible dermal edema and inflammation, while injection of Apx toxins alone did not produce significant macroscopic lesions. However, the repeatability and discreteness of skin lesions induced by App or Apx toxins were insufficient for a robust experimental model that could be used for assessing novel interventions.
JOURNAL OF COMPARATIVE PATHOLOGY
(2022)
Article
Microbiology
Tian Luan, Lu Wang, Jiyu Zhao, Hui Luan, Yueling Zhang, Chunlai Wang, Paul R. Langford, Siguo Liu, Wanjiang Zhang, Gang Li
Summary: In this study, a rapid detection platform called Card was developed for Actinobacillus pleuropneumoniae, which shows high sensitivity and specificity. The detection process is fast, and the results are consistent with traditional PCR methods. Card has potential applications in both diagnostic laboratories and field settings.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Microbiology
Peiru Chen, Chuntong Bao, Rining Zhu, Jun Wang, Junhui Zhu, Ziheng Li, Fengyang Li, Jingmin Gu, Xin Feng, Na Li, Liancheng Lei
Summary: This study investigates the immunological role of interleukin 5 (IL-5) in Actinobacillus pleuropneumoniae (APP) infection in mice. The results show that IL-5 enhances the resistance to APP infection and its deficiency leads to severe clinical symptoms, increased bacterial load, altered immune cell populations, and reduced neutrophil germicidal ability. This research provides new insights for the prevention and treatment of APP or similar respiratory infections.
VETERINARY MICROBIOLOGY
(2022)
Article
Microbiology
Gun Srijuntongsiri, Atiwat Mhoowai, Sukuma Samngamnim, Pornchalit Assavacheep, Janine T. Bosse, Paul R. Langford, Navaporn Posayapisit, Ubolsree Leartsakulpanich, Warangkhana Songsungthong
Summary: In this study, 7 novel A. pleuropneumoniae-specific DNA markers were identified through genome mining and molecular methods. These markers can serve as additional or alternative targets for disease diagnostics, potentially improving disease control.
MICROBIOLOGY SPECTRUM
(2022)
Article
Veterinary Sciences
Rea Jarosova, Petra Ondrackova, Lenka Leva, Katerina Nedbalcova, Monika Vicenova, Josef Masek, Jiri Volf, Jan Gebauer, Tomas Do, Roman Guran, Zbysek Sladek, Javier Dominguez, Martin Faldyna
Summary: CD163+ monocytes contribute to the production of inflammatory cytokines during APP-induced lung inflammation, especially IL-1 beta, at the site of inflammation. PB and BM CD163+ monocytes do not appear to play a significant role in elevated cytokine levels in plasma.
RESEARCH IN VETERINARY SCIENCE
(2022)
Article
Microbiology
Giarla Cunha da Silva, Jessica Nogueira Rosa, Patricia Pereira Fontes, Alex Gazolla de Castro, everton De Almeida Alves Barbosa, Wellington Ronildo Clarindo, Hilario Cuquetto Mantovani, Yanwen Li, Janine Therese Bosse, Paul Richard Langford, Denise Mara Soares Bazzolli
Summary: EVs produced by Actinobacillus pleuropneumoniae contain sRNAs, including those involved in pathogenesis.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Veterinary Sciences
Gabor Kardos, Rita Sarkozi, Levente Laczko, Szilvia Marton, Laszlo Makrai, Krisztian Banyai, Laszlo Fodor
Summary: This study characterized 114 isolates of Actinobacillus pleuropneumoniae from porcine hemorrhagic necrotic pleuropneumonia by examining their biotype, serovar, antibiotic resistance genes, and genes of toxin production. The isolates were found to form 16 clusters based on their genetic relationship, and serovars 2, 13, 9, and 16 were the most frequent. Serovar 13 stood out with its high frequency and low diversity, which were possibly facilitated by a high frequency of resistance to beta-lactams and tetracyclines. The emergence of serovar 16 was likely due to its high virulence.
VETERINARY SCIENCES
(2022)
Article
Ecology
Emmo Hamann, Halina E. Tegetmeyer, Dietmar Riedel, Sten Littmann, Soeren Ahmerkamp, Jianwei Chen, Philipp F. Hach, Marc Strous
Article
Ecology
Hannah K. Marchant, Soeren Ahmerkamp, Gaute Lavik, Halina E. Tegetmeyer, Jon Graf, Judith M. Klatt, Moritz Holtappels, Eva Walpersdorf, Marcel M. M. Kuypers
Article
Ecology
Jianwei Chen, Anna Hanke, Halina E. Tegetmeyer, Ines Kattelmann, Ritin Sharma, Emmo Hamann, Theresa Hargesheimer, Beate Kraft, Sabine Lenk, Jeanine S. Geelhoed, Robert L. Hettich, Marc Strous
Article
Microbiology
Hannah Schramke, Vera Laermann, Halina E. Tegetmeyer, Andreas Brachmann, Kirsten Jung, Karlheinz Altendorf
Article
Microbiology
Viola Krukenberg, Dietmar Riedel, Harald R. Gruber-Vodicka, Pier Luigi Buttigieg, Halina E. Tegetmeyer, Antje Boetius, Gunter Wegener
ENVIRONMENTAL MICROBIOLOGY
(2018)
Article
Microbiology
Hannah K. Marchant, Halina E. Tegetmeyer, Soeren Ahmerkamp, Moritz Holtappels, Gaute Lavik, Jon Graf, Frank Schreiber, Marc Mussmann, Marc Strous, Marcel M. M. Kuypers
ENVIRONMENTAL MICROBIOLOGY
(2018)
Article
Immunology
Yanwen Li, John Spiropoulos, William Cooley, Jasmeet Singh Khara, Camilla A. Gladstone, Masanori Asai, Janine T. Bosse, Brian D. Robertson, Sandra M. Newton, Paul R. Langford
Article
Multidisciplinary Sciences
Danice M. Luvizott, Juliana E. Araujo, Michele De Cassia P. Silva, Armando C. F. Dias, Beate Kraft, Halina Tegetmeye, Marc Strous, Fernando D. Andreote
ANAIS DA ACADEMIA BRASILEIRA DE CIENCIAS
(2019)
Article
Biotechnology & Applied Microbiology
Greta Reintjes, Halina E. Tegetmeyer, Miriam Buergisser, Sandi Orlic, Ivo Tews, Mikhail Zubkov, Daniela Voss, Oliver Zielinski, Christian Quast, Frank Oliver Gloeckner, Rudolf Amann, Timothy G. Ferdelman, Bernhard M. Fuchs
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2019)
Article
Ecology
Adrien Assie, Nikolaus Leisch, Dimitri Meier, Harald Gruber-Vodicka, Halina E. Tegetmeyer, Anke Meyerdierks, Manuel Kleiner, Tjorven Hinzke, Samantha Joye, Matthew Saxton, Nicole Dubilier, Jillian M. Petersen
Article
Microbiology
Rafael Laso-Perez, Cedric Hahn, Daan M. van Vliet, Halina E. Tegetmeyer, Florence Schubotz, Nadine T. Smit, Thomas Pape, Heiko Sahling, Gerhard Bohrmann, Antje Boetius, Katrin Knittel, Gunter Wegener
Article
Microbiology
Rebecca Ansorge, Stefano Romano, Lizbeth Sayavedra, Miguel Angel Gonzalez Porras, Anne Kupczok, Halina E. Tegetmeyer, Nicole Dubilier, Jillian Petersen
NATURE MICROBIOLOGY
(2019)
Article
Microbiology
Eduard Fadeev, Magda G. Cardozo-Mino, Josephine Z. Rapp, Christina Bienhold, Ian Salter, Verena Salman-Carvalho, Massimiliano Molari, Halina E. Tegetmeyer, Pier Luigi Buttigieg, Antje Boetius
Summary: Microbial communities in the Arctic Ocean are still poorly understood, but recent studies have shown the presence of unique microbial community members adapted to extreme environmental conditions. In comparing two commonly used primer sets (V3-V4 and V4-V5) for studying microbial communities in Arctic marine habitats, it was found that both sets represent the total microbial community composition effectively down to the genus rank, with the V4-V5 primer set providing coverage of both bacterial and archaeal domains for a more comprehensive study.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Immunology
Susan L. Brockmeier, Crystal L. Loving, Tracy L. Nicholson, Jinhong Wang, Sarah E. Peters, Lucy Weinert, Roy Chaudhuri, David J. Seilly, Paul R. Langford, Andrew Rycroft, Brendan W. Wren, Duncan J. Maskell, Alexander W. Tucker
INFECTION AND IMMUNITY
(2018)
Article
Immunology
Ming-Shi Li, Paul R. Langford, J. Simon Kroll
INFECTION AND IMMUNITY
(2017)