Article
Acoustics
Antria Filippou, Christakis Damianou
Summary: Experimental measurements of acoustic attenuation and propagation speed were conducted on three surgically excised malignant canine mammary tumours. The results showed that both attenuation and propagation speed of CMTs were higher than normal canine tissues, indicating potential applications of ultrasound in CMTs diagnosis and treatment.
Article
Veterinary Sciences
Yufan Zhu, Baochun Lu, Juye Wu, Shoujun Li, Kun Jia
Summary: This study analyzed the expression profile of circRNAs in canine mammary tumors and identified potential circRNAs that could be used as biomarkers for molecular diagnosis.
VETERINARY SCIENCES
(2022)
Article
Oncology
Yang Zhao, Jia Li, Ka Ka Ting, Jinbiao Chen, Paul Coleman, Ken Liu, Li Wan, Thorleif Moller, Mathew A. Vadas, Jennifer R. Gamble
Summary: The stabilization of VE-Cadherin through CD5-2 treatment selectively regulates leukocyte transmigration, promoting the penetration of CD8(+) T cells while reducing the infiltration of neutrophils. This effect is mediated in part through the upregulation of chemokines like CCL2 and CXCL10, which facilitate immune cell transmigration, and involves the activation of the AKT/GSK3 beta/beta-catenin/TCF4 signaling pathway. Additionally, CD5-2 treatment induces cytoskeletal reorganization and promotes the transcellular migration of CD8(+) T cells by stabilizing the endothelial barrier.
Article
Cell Biology
Qianwen Wang, Chenxiang Qi, Pengxiang Min, Yueyuan Wang, Fengwen Ye, Tianxiang Xia, Yujie Zhang, Jun Du
Summary: By regulating MICAL2, the migratory ability of gastric cancer cells can be influenced, and it involves changes in the E-cadherin/beta-catenin signaling pathway.
CELL COMMUNICATION AND SIGNALING
(2022)
Article
Veterinary Sciences
A. Alonso-Diez, V. K. Affolter, N. Sevane, S. Dunner, G. Valdivia, M. Clemente, P. J. De Andres, J. C. Illera, M. D. Perez-Alenza, L. Pena
Summary: This study found that the expression of E-cadherin and CADM1 was higher in canine inflammatory mammary cancer (IMC) cases, and their expression was associated with exacerbated angiogenesis and lymphangiogenesis factors. The upregulation of CADM1 suggests its potential role in the pathogenesis of IMC. The study highlights the importance of adhesion molecules in the metastatic process and angiogenesis in human inflammatory breast cancer (IBC) and IMC.
RESEARCH IN VETERINARY SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Mehran Erfani, Mozhdeh Zamani, Seyed Younes Hosseini, Zohreh Mostafavi-Pour, Sayed Mohammad Shafiee, Mohammadreza Saeidnia, Pooneh Mokarram
Summary: Our study reveals a close correlation between E-cadherin levels and ARID1A expression, suggesting that ARID1A downregulation may promote CRC metastasis through decreasing E-cadherin expression and enhancing epithelial cell movement. ARID1A could be a potential therapeutic target for CRC treatment.
MOLECULAR BIOLOGY REPORTS
(2021)
Article
Biochemistry & Molecular Biology
Li Han, Huaiqing Luo, Wenjie Huang, Jiang Zhang, Di Wu, Jinmei Wang, Jiao Pi, Chi Liu, Xiangping Qu, Huijun Liu, Xiaoqun Qin, Yang Xiang
Summary: Persistent injury and improper repair in bronchial epithelial cells contribute to airway inflammation and remodeling in asthma. E-cadherin plays a role in regulating the balance between EMT and MET during injury repair by inhibiting EMT features, enhancing MET features, and decreasing TGF-beta 1 secretion and Snail expression, ultimately facilitating epithelial repair.
Article
Veterinary Sciences
Jose Rodriguez, Angelo Santana, Pedro Herraez, David R. Killick, Antonio Espinosa de los Monteros
Summary: This study provides an insight into the changing epidemiology of canine mammary cancer in the Canary Archipelago, showing a high prevalence of malignant tumors. Neutering was negatively associated with epithelial mammary gland tumors, and native breeds in the region had a lower risk of mammary tumors. Further analysis of environmental, socioeconomic, and management factors will enhance understanding of mammary gland tumor epidemiology in both canine and human populations.
BMC VETERINARY RESEARCH
(2022)
Article
Medicine, Research & Experimental
Xinqiu Li, Zixiang Lin, Penglong Wang, Chaoyu Zhou, Jiawei Xu, Jiahao Lin, Degui Lin, Di Zhang
Summary: This study evaluated the efficacy of Tetramethylpyrazine-Rhein Derivative (TRD) on canine inflammatory mammary carcinoma (CIMC) and found that TRD showed potential activity to inhibit CIMC lung metastasis with multi-target and low toxicity.
BIOMEDICINE & PHARMACOTHERAPY
(2023)
Article
Cell Biology
Shiyang Chen, Yajuan Zheng, Xiaojuan Ran, Hui Du, Hua Feng, Lei Yang, Yating Wen, Changdong Lin, Shihui Wang, Mengwen Huang, Zhanjun Yan, Dianqing Wu, Hongyan Wang, Gaoxiang Ge, An Zeng, Yi Arial Zeng, Jianfeng Chen
Summary: T cell contact with intestinal stem cells and transient amplifying cells plays a crucial role in regulating ISC differentiation by influencing the Wnt and Notch signaling pathways through cell-cell signaling. This interaction is essential in maintaining intestinal homeostasis and highlighting the importance of immune cells in intestinal function.
Article
Medicine, Research & Experimental
Wenbei Ma, Zhengyuan Xie, Hui Chen, Lina Zeng, Xiaohong Chen, Songfu Feng, Xiaohe Lu
Summary: High glucose levels in diabetic corneal epithelial cells lead to abnormalities in cell junctions, with decreased E-cadherin expression and increased levels of beta-catenin in the nucleus. This disruption of the beta-catenin/E-cadherin complex promotes nuclear translocation of beta-catenin and activates the transcription and expression of matrix metallopeptidase and snail, interfering with cell adhesion. Targeting the stability of the beta-catenin/E-cadherin complex may provide a potential therapeutic approach for refractory corneal ulcers in diabetic patients.
EXPERIMENTAL BIOLOGY AND MEDICINE
(2021)
Article
Veterinary Sciences
Ana R. Flores, Alexandra Rema, Joao R. Mesquita, Marian Taulescu, Fernanda Seixas, Fatima Gartner, Irina Amorim
Summary: This study found that abnormal expression of E-cadherin is associated with canine gastric cancer, and it may play a role in tumor progression and serve as a prognostic tool.
VETERINARY SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Chang-Gun Lee, Soonjae Hwang, Sun-Yeong Gwon, Chanoh Park, Minjeong Jo, Ju-Eun Hong, Ki-Jong Rhee
Summary: Researchers have found that the secretion of BFT by ETBF can induce IL-8 secretion in intestinal epithelial cells by activating the E-cadherin and beta-catenin signaling pathways, leading to acute inflammation.
Article
Veterinary Sciences
Giada Giambrone, Stefania Di Giorgio, Cecilia Vullo, Gabriele Marino, Roberto Puleio, Francesca Mariotti, Giuseppe Mazzullo, Alessandra Sfacteria
Summary: This study demonstrates the existence of TLSs in canine mammary simple carcinomas, where some inflammatory infiltrates assume a follicle-like organization, especially in high-grade carcinomas. The role of TLSs in tumors is controversial, but their presence in aggressive tumor subtypes suggests their importance in tumor progression.
VETERINARY SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Lin Shen, Jing Zhang, Meng Xu, Ying Zheng, Mo Wang, Suzhen Yang, Bin Qin, Shunle Li, Lei Dong, Fei Dai
Summary: DDX3 functions as a tumor suppressor gene in colorectal cancer. Loss of DDX3 expression promotes cancer progression by regulating E-cadherin and beta-catenin signaling through the MAPK pathway. Targeting the MAPK pathway may be a therapeutic approach for advanced colorectal cancer.
INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES
(2022)
Editorial Material
Pathology
Adhemar Longattho Filho, Fernando C. Schmitt
Article
Engineering, Biomedical
Francisco Conceicao, Daniela M. Sousa, Joshua Loessberg-Zahl, Anke R. Vollertsen, Estrela Neto, Kent Soe, Joana Paredes, Anne Leferink, Meriem Lamghari
Summary: This study introduced a novel humanized organ-on-a-chip model recapitulating neuro-breast cancer crosstalk in a bone metastatic context. The research revealed that synergistic paracrine signaling from sympathetic neurons and osteoclasts increase breast cancer aggressiveness.
MATERIALS TODAY BIO
(2022)
Review
Cell Biology
Emad A. Rakha, Ewa Chmielik, Fernando C. Schmitt, Puay Hoon Tan, Cecily M. Quinn, Grace Gallagy
Summary: The management of breast cancer patients relies on the assessment of prognostic and predictive markers. This review provides an update on the assessment of key molecular markers in breast cancer, including hormone receptors, HER2, and KI67, and highlights some challenges and focus areas.
Article
Medical Laboratory Technology
Fernando Schmitt, Maria D. Lozano
Summary: The understanding of molecular mechanisms in lung cancer and the discovery of targetable genomic alterations have significantly changed the pathological approach to lung cancer, particularly non-small cell lung cancer (NSCLC). Pathologists have been at the forefront of lung cancer management due to this unstoppable knowledge. In the field of cytopathology, where maximizing output with minimal input is a daily challenge, there has been a paradigm shift in the care and handling of diagnostic samples. This shift emphasizes the utilization of cytologic samples and small biopsies for both diagnostic and ancillary testing purposes.
DIAGNOSTIC CYTOPATHOLOGY
(2023)
Article
Pathology
Fernando C. Schmitt, Lukas Bubendorf, Sule Canberk, Ashish Chandra, Ian A. Cree, Marianne Engels, Kenzo Hiroshima, Deepali Jain, Ivana Kholova, Lester Layfield, Ravi Mehrotra, Claire W. Michael, Robert Osamura, Martha B. Pitman, Sinchita Roy-Chowdhuri, Yukitoshi Satoh, Paul VanderLaan, Maureen F. Zakowski, Andrew S. Field
Summary: The International Academy of Cytology and the International Agency for Research on Cancer have collaborated to develop a WHO Reporting System for Lung Cytopathology. This system defines five categories for reporting lung cytopathology and provides a standardized approach for testing and diagnosing lung diseases worldwide. It aims to improve patient care and raise the profile of cytopathology.
Article
Pathology
Paula de Melo Alves, Fernando Ferreira, Telma Oliveira, Daniela Alves, Sule Canberk, Fernando C. Schmitt
Summary: Thyroid fine-needle aspiration (FNA) is a well-established technique for primary diagnosis. This study compared the staining effects of two methods, namely Diff-Quik (DQ) and BlueStain (R), and found that BlueStain (R) showed better preservation of colloid and cell details, making it a more cost-effective and simpler alternative to DQ stain.
Editorial Material
Medical Laboratory Technology
Fernando Schmitt, Zhongbo Yang, Magda Esebua
Summary: In the era of personalized medicine, molecular testing plays a critical role in patient care, particularly in cytology samples. It increases diagnostic sensitivity and specificity and helps select appropriate treatment for patients. This special issue provides readers with more information about molecular cytology through a review of literature and discussions on the basics of molecular techniques.
DIAGNOSTIC CYTOPATHOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Nuno Alegrete, Susana R. R. Sousa, Tatiana Padrao, Angela Carvalho, Raquel Lucas, Raphael F. F. Canadas, Catarina Lavrador, Nuno Alexandre, Fatima Gartner, Fernando J. J. Monteiro, Manuel Gutierres
Summary: The treatment for osteomyelitis usually involves surgical debridement, tissue coverage, and long-term administration of antibiotics. V-HEPHAPC, a composite material containing vancomycin, has shown promising results in vitro as a local antibiotic carrier and bone scaffold. In this study, V-HEPHAPC granules were successfully used to treat MRSA osteomyelitis in a sheep model.
BIOENGINEERING-BASEL
(2023)
Article
Cell Biology
Christopher J. VandenBussche, Barbara Crothers, Ashish Chandra, Fernando Schmitt, Daniel F. I. Kurtycz
Summary: Objective: An international panel conducted a survey to identify opinions and explore existing practice patterns regarding body fluid cytopathology. The survey collected 593 international participant responses, revealing strong support for the development of a unified system for reporting body fluid cytopathology among respondents.
Article
Cell Biology
Lidia Faria, Sara Canato, Tito T. Jesus, Margarida Goncalves, Patricia S. Guerreiro, Carla S. Lopes, Isabel Meireles, Eurico Morais-de-Sa, Joana Paredes, Florence Janody
Summary: Alterations in P-cadherin expression or function influence tumor progression. P-cadherin is highly expressed in basal-like breast carcinomas and is involved in cancer cell renewal, migration, and invasion. In this study, a humanized P-cadherin Drosophila model was generated to investigate its role in vivo. The results showed that actin nucleators Mrtf and Srf are main effectors of P-cadherin in flies. These findings were validated in a human mammary epithelial cell line with conditional activation of the SRC oncogene. Knocking down P-cadherin or inhibiting F-actin polymerization impaired SRF transcriptional activity, while blocking Mrtf-A nuclear translocation hindered proliferation, self-renewal, and invasion.
DISEASE MODELS & MECHANISMS
(2023)
Article
Cell Biology
Irina Fonseca, Cintia Horta, Ana Sofia Ribeiro, Barbara Sousa, Gaelle Marteil, Monica Bettencourt-Dias, Joana Paredes
Summary: Polo-like kinase 4 (Plk4) is a potential therapeutic target in cancer due to its abnormal expression, which leads to centrosome number deregulation and mitotic defects. Plk4 overexpression significantly enhances resistance to anoikis of p53 knock-out mammary epithelial cells and induces a stable hybrid epithelial-mesenchymal phenotype, partially dependent on P-cadherin upregulation.
CELL DEATH & DISEASE
(2023)
Article
Cell Biology
Gisela Magalhaes, Rita Calisto, Catarina Freire, Regina Silva, Diana Montezuma, Sule Canberk, Fernando Schmitt
Summary: Digital pathology is the future for histopathology laboratories, but the implementation process requires workflow reorganisation and often overlooks the role of Histotechnologists (HTLs). This article aims to provide guidance and practical recommendations for HTLs to contribute to the implementation of a new digital workflow, as well as to improve study programs and education regarding HTLs in digital pathology.
JOURNAL OF HISTOTECHNOLOGY
(2023)
Article
Oncology
Carla Luis, Fernando Schmitt, Rute Fernandes, Nuno Coimbra, Joana Rigor, Paula Dias, Dina Leitao, Ruben Fernandes, Raquel Soares
Summary: This study investigated the expression of enzymes involved in glycolysis and gluconeogenesis in breast cancer, and found significant differences based on molecular subtype and body mass index. These differences were closely related to hormonal receptor and HER2 status, as well as pathological stage and histological grade. Obesity appeared to attenuate these differences, but adipocyte deposition and inflammatory infiltration did not affect the differences. Additionally, obesity-related conditions such as diabetes, hypertension, and dyslipidemia influenced the expression of certain enzymes.
Article
Oncology
Ines Brandao Rego, Sara Coelho, Patricia Miguel Semedo, Joana Cavaco-Silva, Laetitia Teixeira, Susana Sousa, Joana Reis, Rui Dinis, Fernando Schmitt, Noemia Afonso, Jose Luis Fougo, Francisco Pavao, Ricardo Baptista Leite, Luis Costa
Summary: This study aims to describe the compliance with EUSOMA indicators in breast cancer management in different hospital settings in Portugal. Although most indicators meet EUSOMA standards, there are differences among institutions, especially in diagnosis and disease stages.
Article
Endocrinology & Metabolism
Cosimo Durante, Laszlo Hegedues, Agnieszka Czarniecka, Ralf Paschke, Gilles Russ, Fernando Schmitt, Paula Soares, Tamas Solymosi, Enrico Papini
Summary: With the widespread use of imaging techniques, an increasing number of thyroid nodules are discovered. Most are benign and do not require treatment, while small intrathyroidal neoplasms can be safely managed conservatively. Guidelines are needed to provide a cost-effective and patient-centered approach to managing this prevalent condition. The present guidelines aim to provide a clinical practice guide for the initial workup and management of thyroid nodules in adults, and are not intended for the management of thyroid malignancy.
EUROPEAN THYROID JOURNAL
(2023)
Article
Veterinary Sciences
L. Alibrandi, R. Tognetti, O. Domenech, M. Croce, M. Giuntoli, G. Grosso, T. Vezzosi
Summary: This study assessed the feasibility and diagnostic reliability of a new smartphone-based ECG device in dogs, and found no significant differences compared to a traditional ECG device. The results suggest that the smartphone-based device is clinically reliable for assessing heart rate and rhythm in dogs.
VETERINARY JOURNAL
(2024)
