Aggregating tags for column-free protein purification

A designed, phase changing RTX-based peptide for efficient bioseparations

(2016) Oren Shur et al.   BIOTECHNIQUES

New trends and affinity tag designs for recombinant protein purification

(2014) David W Wood   CURRENT OPINION IN STRUCTURAL BIOLOGY

Engineering a recyclable elastin-like polypeptide capturing scaffold for non-chromatographic protein purification

(2013) Fang Liu et al.   BIOTECHNOLOGY PROGRESS

Facile expression and purification of the antimicrobial peptide histatin 1 with a cleavable self-aggregating tag (cSAT) in Escherichia coli

(2013) Lei Xing et al.   PROTEIN EXPRESSION AND PURIFICATION

Efficient production of recombinant cystatin C using a peptide-tag, 4AaCter, that facilitates formation of insoluble protein inclusion bodies in Escherichia coli

(2013) Masahiro Hayashi et al.   PROTEIN EXPRESSION AND PURIFICATION

A dual ELP-tagged split intein system for non-chromatographic recombinant protein purification

(2012) Changhua Shi et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Engineering a high-affinity scaffold for non-chromatographic protein purification via intein-mediated cleavage

(2012) Fang Liu et al.   BIOTECHNOLOGY AND BIOENGINEERING

Protein refolding using chemical refolding additives

(2012) Satoshi Yamaguchi et al.   Biotechnology Journal

Recent progress in intein research: from mechanism to directed evolution and applications

(2012) Gerrit Volkmann et al.   CELLULAR AND MOLECULAR LIFE SCIENCES

Pharmaceutical and biomedical applications of affinity chromatography: Recent trends and developments

(2012) David S. Hage et al.   JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS

Expression and purification of the p75 neurotrophin receptor transmembrane domain using a ketosteroid isomerase tag

(2012) Qingxin Li et al.   Microbial Cell Factories

Small surfactant-like peptides can drive soluble proteins into active aggregates

(2012) Bihong Zhou et al.   Microbial Cell Factories

Engineering split intein DnaE from Nostoc punctiforme for rapid protein purification

(2012) M. Ramirez et al.   PROTEIN ENGINEERING DESIGN & SELECTION

A PagP fusion protein system for the expression of intrinsically disordered proteins in Escherichia coli

(2012) Peter M. Hwang et al.   PROTEIN EXPRESSION AND PURIFICATION

Mutational analyses of Cry protein block7 polypeptides that facilitate the formation of protein inclusion in Escherichia coli

(2011) Tohru Hayakawa et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Exploiting the interactions between poly-histidine fusion tags and immobilized metal ions

(2011) Wen-Hui K. Kuo et al.   BIOTECHNOLOGY LETTERS

Self-cleaving fusion tags for recombinant protein production

(2011) Yifeng Li   BIOTECHNOLOGY LETTERS

Comparison of expression, purification and characterization of a new pectate lyase from Phytophthora capsici using two different methods

(2011) Huizheng Wang et al.   BMC BIOTECHNOLOGY

Active protein aggregates induced by terminally attached self-assembling peptide ELK16 in Escherichia coli

(2011) Wei Wu et al.   Microbial Cell Factories

Streamlined protein expression and purification using cleavable self-aggregating tags

(2011) Lei Xing et al.   Microbial Cell Factories

Expression and purification of antimicrobial peptide CM4 by Npro fusion technology in E. coli

(2010) Xiawei Cheng et al.   AMINO ACIDS

Recombinant self-assembling peptides as biomaterials for tissue engineering

(2010) Stuart Kyle et al.   BIOMATERIALS

Novel strategy for protein production using a peptide tag derived from Bacillus thuringiensis Cry4Aa

(2010) Tohru Hayakawa et al.   FEBS Journal

Inclusion bodies: a new concept

(2010) Elena García-Fruitós   Microbial Cell Factories

Production of recombinant isotopically labelled peptide by fusion to an insoluble partner protein: generation of integrin αvβ6 binding peptides for NMR

(2010) Jane L. Wagstaff et al.   Molecular BioSystems

NproAutoprotease Fusion Technology: Development, Characteristics, and Influential Factors

(2010) Astrid Dürauer et al.   SEPARATION SCIENCE AND TECHNOLOGY

Non-Chromatographic Recombinant Protein Purification by Self-Cleaving Purification Tags

(2010) David W. Wood   SEPARATION SCIENCE AND TECHNOLOGY

The potential role of self-cleaving purification tags in commercial-scale processes

(2010) Baley A. Fong et al.   TRENDS IN BIOTECHNOLOGY

Production and isotope labeling of antimicrobial peptides inEscherichia coliby means of a novel fusion partner that enables high-yield insoluble expression and fast purification

(2009) Verica Vidovic et al.   JOURNAL OF PEPTIDE SCIENCE

RTX Calcium Binding Motifs Are Intrinsically Disordered in the Absence of Calcium

(2008) Alexandre Chenal et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Expression and purification of recombinant proteins from Escherichia coli: Comparison of an elastin-like polypeptide fusion with an oligohistidine fusion

(2004) Kimberly Trabbic-Carlson et al.   PROTEIN SCIENCE