期刊
PROTEIN EXPRESSION AND PURIFICATION
卷 88, 期 2, 页码 230-234出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.pep.2013.01.011
关键词
Recombinant cystatin C; Biomarker for renal dysfunction; Production of antigen protein; Bacillus thuringiensis; Cry4Aa toxin; 4AaCter-tag; Formation of insoluble protein inclusion bodies
类别
资金
- JSPS KAKENHI [20380036, 23658048, 24380034]
- Grants-in-Aid for Scientific Research [20380036, 23658048] Funding Source: KAKEN
Cystatin C is a cysteine protease inhibitor produced by a variety of human tissues. The blood concentration of cystatin C depends on the glomerular filtration rate and is an endogenous marker of renal dysfunction. Recombinant cystatin C protein with high immunogenicity is therefore in demand for the diagnostic market. In this study, to establish an efficient production system, a synthetic cystatin C gene was designed and synthesized in accordance with the codon preference of Escherichia coli genes. Recombinant cystatin C was expressed as a fusion with a peptide-tag, 4AaCter, which facilitates formation of protein inclusion bodies in E. coli cells. Fusion with 4AaCter-tag dramatically increased the production level of cystatin C, and highly purified protein was obtained without the need for complicated purification steps. The purity and yield of the final product was estimated as 87 +/- 5% and 7.1 +/- 1.1 mg/l culture, respectively. The recombinant cystatin C prepared by our method was as reactive against anti-cystatin C antibodies as native human cystatin C. Our results suggest that protein production systems using 4AaCter-tag could be a powerful means of preparing significant amounts of antigen protein. (C) 2013 Elsevier Inc. All rights reserved.
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