Article
Multidisciplinary Sciences
Wenwei Lin, Xiang Zhou, Wenxin Tang, Koji Takahashi, Xue Pan, Jiawei Dai, Hong Ren, Xiaoyue Zhu, Songqin Pan, Haiyan Zheng, William M. Gray, Tongda Xu, Toshinori Kinoshita, Zhenbiao Yang
Summary: The plant hormone auxin controls various growth processes in plants by regulating cell expansion through activating plasma membrane H+-ATPase through interactions with transmembrane kinase (TMK) auxin-signalling proteins, promoting cell-wall acidification and hypocotyl cell elongation in Arabidopsis.
Article
Multidisciplinary Sciences
Alan Le Mercier, Remy Bonnavion, Weijia Yu, Mohamad Wessam Alnouri, Sophie Ramas, Yang Zhang, Yannick Jaeger, Kenneth Anthony Roquid, Hyun-Woo Jeong, Kishor Kumar Sivaraj, Haaglim Cho, Xinyi Chen, Boris Strilic, Tjeerd Sijmonsma, Ralf Adams, Timm Schroeder, Michael A. Rieger, Stefan Offemanns
Summary: GPR182 is a new atypical chemokine receptor for CXCL10, CXCL12, and CXCL13, involved in regulating hematopoietic stem cell homeostasis.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Oncology
Hitendra S. Solanki, Eric A. Welsh, Bin Fang, Victoria Izumi, Lancia Darville, Brandon Stone, Ryan Franzese, Sandip Chavan, Fumi Kinose, Denis Imbody, John M. Koomen, Uwe Rix, Eric B. Haura
Summary: The study reveals cell type-specific adaptive responses to KRASi, with epithelial cells compensating ERBB2/3 signaling and mesenchymal cells showing activation of FGFR or AXL signaling. These findings suggest the importance of considering cell type-specific markers and targets for patient enrichment strategies with KRASi.
CLINICAL CANCER RESEARCH
(2021)
Article
Oncology
Kimberley C. Clark, Elizabeth V. Nguyen, Birunthi Niranjan, Yunjian Wu, Terry C. C. Lim Kam Sian, Lisa G. Horvath, Renea A. Taylor, Roger J. Daly
Summary: Prostate cancer is a common cancer in men and a major cause of cancer-related deaths. In this study, we investigated the cell-type-specific signaling networks initiated by intercellular communication between prostate epithelial cells and patient-derived cancer-associated fibroblasts (CAFs) when grown together. Significant differences in proteomic profiles were identified, and the upregulation of transglutaminase-2 (TGM2) was found to promote migration and proliferation of epithelial cells under co-culture conditions. These findings provide insights into intercellular communication in prostate cancer that may improve patient management.
Article
Chemistry, Analytical
Aaron James F. Reyes, Reta Birhanu Kitata, Mira Anne C. dela Rosa, Yi-Ting Wang, Pei-Yi Lin, Pan-Chyr Yang, Assaf Friedler, Shlomo Yitzchaik, Yu-Ju Chen
Summary: The dysregulation of phosphorylation-mediated signaling plays a critical role in the development of diseases. A sensitive and rapid substrate-specific kinase assay has been developed using site-specific peptide reporter and MRM-MS platform, allowing for precise monitoring of disease states. This approach offers a rapid and sensitive method for targeted quantification of substrate-specific kinase activity in small amounts of samples, with high accuracy and reproducibility.
ANALYTICA CHIMICA ACTA
(2021)
Article
Pharmacology & Pharmacy
Yuhong Jiang, Junxian Lim, Kai-Chen Wu, Weijun Xu, Jacky Y. Suen, David P. Fairlie
Summary: This study reveals a novel shared signalling cascade involving Gα(q/11), Gα(12/13), and beta-arrestin1/2 for PAR2-induced ovarian cancer cell migration and invasion, resulting in downstream serial activation of Src kinases, transactivation of EGFR, and subsequent MEK-ERK1/2-FOS/MYC/STAT3-COX2 signalling, which is not dependent on a single G protein or beta-arrestin-mediated pathway.
BRITISH JOURNAL OF PHARMACOLOGY
(2021)
Article
Gastroenterology & Hepatology
Ozge Kayisoglu, Franziska Weiss, Carolin Niklas, Isabella Pierotti, Malvika Pompaiah, Nina Wallaschek, Christoph-Thomas Germer, Armin Wiegering, Sina Bartfeld
Summary: The study demonstrates extensive spatial organization of innate immune signaling components along the cephalocaudal axis in the GI tract. This organization is largely determined before birth and independent of exposure to commensal gut microbiota. Gastric epithelial organoids can sense LPS from both the basal and apical sides.
Article
Biochemistry & Molecular Biology
Hongliang Wang, Yubei Wang, Tian Sang, Zhen Lin, Rongxia Li, Weiwei Ren, Xin Shen, Bing Zhao, Xiao Wang, Xuebin Zhang, Shaoqun Zhou, Shaojun Dai, Honghong Hu, Chun-Peng Song, Pengcheng Wang
Summary: Multicellular organisms, like plants, have different cell types with specialized functions. This study used proteomics analysis to analyze guard cells (GCs), which are specialized epidermal cells regulating stomata movement and gaseous exchange. The researchers identified new proteins in the GC proteome and discovered a kinase cascade specific to guard cells, which explains how abscisic acid (ABA) activates stomatal closure. Enrichment of enzymes and flavone metabolism in GCs was also observed. This study provides insights into GC and mesophyll cell development, metabolism, structure, and function.
JOURNAL OF INTEGRATIVE PLANT BIOLOGY
(2023)
Article
Multidisciplinary Sciences
John A. Halsall, Simon Andrews, Felix Krueger, Charlotte E. Rutledge, Gabriella Ficz, Wolf Reik, Bryan M. Turner
Summary: The study revealed that chromatin configuration significantly influences gene expression in eukaryotes, with specific histone modifications exhibiting characteristic genomic distributions at resolutions of 1 MB and below. These patterns differ between HeLa and lymphoblastoid cells, but remain consistent throughout the cell cycle.
SCIENTIFIC REPORTS
(2021)
Article
Chemistry, Multidisciplinary
Silvia Arifi, Julian A. Marschner, Julius Pollinger, Laura Isigkeit, Pascal Heitel, Astrid Kaiser, Lennart Obeser, Georg Hoefner, Ewgenij Proschak, Stefan Knapp, Apirat Chaikuad, Jan Heering, Daniel Merk
Summary: The lipid-sensing transcription factor PPAR γ can bind to antidiabetic TZD drugs, oxidized vitamin E metabolites, and vitamin E mimetic garcinoic acid. While the effects of the second binding on PPAR γ activity are unclear, a selective ligand of the second site has been developed, revealing potential noncanonical regulation of PPAR γ activities. This alternative binding diminishes FOXO signaling and may have therapeutic applications.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2023)
Article
Multidisciplinary Sciences
Lanxin Li, Inge Verstraeten, Mark Roosjen, Koji Takahashi, Lesia Rodriguez, Jack Merrin, Jian Chen, Lana Shabala, Wouter Smet, Hong Ren, Steffen Vanneste, Sergey Shabala, Bert De Rybel, Dolf Weijers, Toshinori Kinoshita, William M. Gray, Jiri Friml
Summary: Plant growth is regulated by environmental factors, such as how plants respond to gravity by bending shoots up and roots down. Research has shown that the plant hormone auxin affects root growth by regulating cell expansion and contraction in the roots.
Article
Multidisciplinary Sciences
Marie-Estelle Losfeld, Ernesto Scibona, Chia-wei Lin, Markus Aebi
Summary: Glycoprotein processing is a complex and compartmentalized pathway in eukaryotic cells. We studied the kinetics of glycan processing of a model protein and developed a mathematical model of the secretory pathway. Our model revealed the importance of phosphorylated glycan structures in lysosomal sorting and showed that the model protein can take different routes of the cellular secretory pathway, resulting in increased glycan complexity of the secreted protein.
Article
Chemistry, Multidisciplinary
Zhian Hu, Jinlei Yang, Fujian Xu, Gongwei Sun, Xingyu Pan, Mengchan Xia, Sichun Zhang, Xinrong Zhang
Summary: The study discovered that myeloperoxidase can accurately cleave phosphorothioate-modified DNA to activate DNA machines for specific functions. This tool enables robust imaging of oxidative stress in living cells, providing a new approach for research on biostimuli and construction of DNA molecular devices.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2021)
Article
Biochemistry & Molecular Biology
Ran Ke, Samson Ian Sam Lok, Kailash Singh, Billy Kwok Chong Chow, Harald Janovjak, Leo Tsz On Lee
Summary: The study reveals that the formation of heterocomplexes between Kiss1R and GPER significantly reduces Kiss1R-mediated signaling, showcasing a regulatory mechanism of hypothalamic hormone receptors through receptor cooperation and modulation of receptor sensitivity in the reproductive system.
JOURNAL OF MOLECULAR BIOLOGY
(2021)
Review
Plant Sciences
Andras Viczian, Ferenc Nagy
Summary: The phytochrome B (phyB) photoreceptor plays a crucial role in red and far-red light sensing in plants, governing various signaling pathways. Phosphorylation is an important regulatory mechanism for phyB, but it is challenging to examine. Recent studies have identified the functional role of phosphorylated phyB residues and the enzymes that modulate its phospho-state.
