Review
Cell Biology
Marta Gomarasca, Giovanni Lombardi, Paola Maroni
Summary: Post-translational modifications, such as SUMOylation and NEDDylation, have significant roles in tumor cell biology, influencing various aspects of neoplastic transformation and evolution. This article focuses on the involvement and regulation of SUMOylation and NEDDylation in primary and secondary bone tumors, highlighting their potential in diagnostics and therapeutic strategies.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Cell Biology
Juan F. Correa-Vazquez, Francisco Juarez-Vicente, Pablo Garcia-Gutierrez, Sina Barysch, Frauke Melchior, Mario Garcia-Dominguez
Summary: Post-translational modification through Sumo plays a crucial role in regulating neurogenesis. The sumoylation of key proteins can either positively or negatively affect the process of neurogenesis, highlighting the importance of balanced sumoylation for proper development.
CELL DEATH & DISEASE
(2021)
Review
Biochemistry & Molecular Biology
Ishita Tripathi-Giesgen, Christian Behrends, Arno F. Alpi
Summary: The ubiquitin system plays a crucial role in the host cellular defense program against bacterial infection, especially when certain bacteria are exposed to the host cytosol during invasion. Host cell E3 ubiquitin ligases contribute to the formation of a protective ubiquitin coat on invading pathogens, with their divergent ubiquitin conjugation mechanisms influencing the complexity of the anti-bacterial coating. Bacteria have evolved strategies to evade the activities of the host ubiquitin system.
Article
Microbiology
Min Zhao, Yaolin Zhang, Xiqin Yang, Jiayang Jin, Zhuo Shen, Xiaoyao Feng, Tao Zou, Lijiao Deng, Daohai Cheng, Xueting Zhang, Cheng Qin, Chunxiao Niu, Zhenjie Ye, Xueying Zhang, Jia He, Chunmei Hou, Ge Li, Gencheng Han, Qianqian Cheng, Qingyang Wang, Lin Wei, Jie Dong, Jiyan Zhang
Summary: Neddylation plays a crucial role in promoting RNA virus-induced type I IFN production and enhancing the transcriptional activity of IRF7, contributing to host anti-viral innate immunity. The blocking of neddylation impedes RNA virus-induced IRF7 nuclear translocation, leading to reduced IFN-alpha production and impaired activation of Ifna gene promoters.
Review
Biochemistry & Molecular Biology
Wieslaw Swietnicki
Summary: The review discusses strategies for designing vaccines against selected pathogens using bacterial secretory systems and connects them to the biology of the system. Examples are given for Y. pestis, S. enterica, B. anthracis, S. flexneri, and other human pathogens, and their effectiveness and long-term protection are discussed.
Article
Biochemistry & Molecular Biology
Daniel Salas-Lloret, Roman Gonzalez-Prieto
Summary: Ubiquitination and SUMOylation are dynamic post-translational modifications that regulate cellular processes. The understanding of these modifications has been increased by the development of mass spectrometry-based approaches. This translated article provides a concise overview of the basic mechanisms of ubiquitination and SUMOylation, as well as recent MS-based approaches for specific target identification.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Wenjuan Zhang, Lihui Li, Lili Cai, Yupei Liang, Junfeng Xu, Yue Liu, Lisha Zhou, Chen Ding, Yanmei Zhang, Hu Zhao, Jun Qin, Zhimin Shao, Wenyi Wei, Lijun Jia
Summary: In this study, it was discovered that Prame recognizes p14/ARF for degradation. Inhibiting Prame expression and halting p14/ARF degradation can restrain tumor growth and induce G2/M phase cell cycle arrest.
CELL DEATH AND DIFFERENTIATION
(2021)
Article
Cell Biology
Immacolata Giordano, Lucia Pirone, Veronica Muratore, Eukene Landaluze, Coralia Perez, Valerie Lang, Elisa Garde-Lapido, Monika Gonzalez-Lopez, Orhi Barroso-Gomila, Alfred C. O. Vertegaal, Ana M. Aransay, Jose Antonio Rodriguez, Manuel S. Rodriguez, James D. Sutherland, Rosa Barrio
Summary: The study investigates the interaction between two key transcription factors, CBX4 and SALL1, and reveals that increased SALL1 expression reduces the ubiquitination of CBX4, enhancing its stability. This leads to an increase in the number and size of CBX4-containing Polycomb bodies, resulting in greater repression of CBX4 target genes.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Audrey Dangoumau, Sylviane Marouillat, Roxane Coelho, Francois Wurmser, Celine Brulard, Shanez Haouari, Frederic Laumonnier, Philippe Corcia, Christian R. Andres, Helene Blasco, Patrick Vourc'h
Summary: Protein aggregates in motor neurons affected by ALS are associated with oxidative stress, which alters the expression of genes involved in ubiquitin and SUMO pathways. The combined effects of oxidative stress and ALS-related genetic mutations on gene expression highlight potential mechanisms underlying ALS pathophysiology and suggest targets for protecting motor neurons from neurodegeneration.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Editorial Material
Biochemistry & Molecular Biology
Xiaodong Cheng
Summary: Protein SUMOylation is an essential post-translational modification for maintaining cellular homeostasis. Recent evidence suggests that liquid-liquid phase separation (LLPS)/ biomolecular condensates play a role in regulating cellular SUMOylation, providing insights into its cellular mechanism.
TRENDS IN BIOCHEMICAL SCIENCES
(2023)
Article
Multidisciplinary Sciences
Jie Li, Gregory J. Krause, Qi Gui, Susmita Kaushik, Gergely Rona, Qingyue Zhang, Feng-Xia Liang, Avantika Dhabaria, Carlos Anerillas, Jennifer L. Martindale, Nikita Vasilyev, Manor Askenazi, Beatrix Ueberheide, Evgeny Nudler, Myriam Gorospe, Ana Maria Cuervo, Michele Pagano
Summary: This study identifies a new function for mammalian SKP1 in the endolysosomes, which is independent of its established role as a component of ubiquitin ligases. SKP1 is found to be phosphorylated under nutrient-poor conditions, promoting V-ATPase assembly and acidification of late endosomes for degradation. Under nutrient-rich conditions, phosphorylated SKP1 is SUMOylated and allows unconventional secretion through interaction with SEC22B.
Article
Microbiology
Hilary A. Phelps, Michael Kuhn, Yanran Lu, Sandip Vibhute, Jeffrey L. Watts, Mark J. Mitton-Fry
Summary: Multidrug-resistant bacteria not only affect human health, but also infect companion animals and livestock. Novel Bacterial Topoisomerase Inhibitors (NBTIs) have shown excellent activity against Gram-positive bacteria and are promising new antibacterial agents. In this study, we evaluate the antibacterial activity of these NBTIs against various important veterinary pathogens and demonstrate outstanding in vitro activity, especially against staphylococci.
VETERINARY MICROBIOLOGY
(2023)
Review
Cell Biology
Fumiyo Ikeda
Summary: Ubiquitin modifies diverse substrates through various conjugation types, regulating a wide range of biological functions. While the C-terminus of ubiquitin traditionally forms isopeptide or peptide bonds with protein substrates, recent studies have revealed that it can also form atypical oxyester bonds, targeting both proteinaceous and nonproteinaceous substrates such as sugars and lipids. The understanding of how nonprotein ubiquitination affects substrate and cellular functions is still incomplete. This review discusses recent discoveries in ubiquitination and its potential impacts on biology.
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
(2023)
Article
Multidisciplinary Sciences
Hyein G. Lee, Abigail A. Lemmon, Christopher D. Lima
Summary: The Ufd1/Npl4/Cdc48 complex enhances unfolding of substrates modified by SUMO-polyubiquitin hybrid chains through interactions with SUMO. It exhibits a stronger effect on substrates modified by these chains compared to polyubiquitin chains when given a choice. Cryo-EM structures reveal the interactions between Ufd1/Npl4/Cdc48 and ubiquitin during the unfolding process. These findings confirm the significance of SUMO and ubiquitin modifications in cellular functions and support a model where Ufd1/Npl4/Cdc48, SUMO, and ubiquitin conjugation pathways converge to promote clearance of proteins modified with SUMO and polyubiquitin.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Review
Virology
Laurie-Anne Lamotte, Lionel Tafforeau
Summary: The review discusses how the NS1 protein of influenza A virus inhibits the host's antiviral response by disturbing ubiquitination.
Review
Immunology
David Ribet, Pascale Cossart
MICROBES AND INFECTION
(2015)
Article
Cell Biology
Julien Karim Malet, Pascale Cossart, David Ribet
CELLULAR MICROBIOLOGY
(2017)
Article
Cell Biology
David Ribet, Serena Boscaini, Clothilde Cauvin, Martin Siguier, Serge Mostowy, Arnaud Echard, Pascale Cossart
JOURNAL OF CELL BIOLOGY
(2017)
Article
Microbiology
David Ribet, Valerie Lallemand-Breitenbach, Omar Ferhi, Marie-Anne Nahori, Hugo Varet, Hugues de The, Pascale Cossart
Article
Biochemical Research Methods
Julien Karim Malet, Francis Impens, Filipe Carvalho, Melanie Anne Hamon, Pascale Cossart, David Ribet
MOLECULAR & CELLULAR PROTEOMICS
(2018)
Article
Virology
Jhen Tsang, David Ribet, Thierry Heidmann, Marie Dewannieux
JOURNAL OF VIROLOGY
(2019)
Article
Nutrition & Dietetics
Jonathan Breton, Pauline Tirelle, Soujoud Hasanat, Arthur Pernot, Clement L'Huillier, Jean-Claude do Rego, Pierre Dechelotte, Moise Coeffier, Laure B. Bindels, David Ribet
Summary: The gut microbiota dysbiosis in the Activity-Based Anorexia mouse model was comprehensively characterized, revealing correlations between bacterial level changes and physiological parameters. Most observed gut microbiota alterations were found to be due to food restriction rather than physical activity. Furthermore, certain bacterial groups were identified to correlate with body weight, food intake, and hypothalamic neuropeptide levels in mice.
CLINICAL NUTRITION
(2021)
Correction
Multidisciplinary Sciences
David Ribet, Melanie Hamon, Edith Gouin, Marie-Anne Nahori, Francis Impens, Helene Neyret-Kahn, Kris Gevaert, Joel Vandekerckhove, Anne Dejean, Pascale Cossart
Article
Biochemistry & Molecular Biology
Wafa Bahlouli, Jonathan Breton, Mauranne Lelouard, Clement L'Huillier, Pauline Tirelle, Emmeline Salameh, Asma Amamou, Karim Atmani, Alexis Goichon, Christine Bole-Feysot, Philippe Ducrotte, David Ribet, Pierre Dechelotte, Moise Coeffier
JOURNAL OF NUTRITIONAL BIOCHEMISTRY
(2020)
Article
Nutrition & Dietetics
Mariona Pinart, Katharina Nimptsch, Sofia K. Forslund, Kristina Schlicht, Miguel Gueimonde, Patrizia Brigidi, Silvia Turroni, Wolfgang Ahrens, Antje Hebestreit, Maike Wolters, Andreas Doetsch, Ute Noethlings, Kolade Oluwagbemigun, Rafael R. C. Cuadrat, Matthias B. Schulze, Marie Standl, Michael Schloter, Maria De Angelis, Patricia Iozzo, Maria Angela Guzzardi, Geertrui Vlaemynck, John Penders, Daisy M. A. E. Jonkers, Maya Stemmer, Giulia Chiesa, Duccio Cavalieri, Carlotta De Filippo, Danilo Ercolini, Francesca De Filippis, David Ribet, Najate Achamrah, Marie-Pierre Tavolacci, Pierre Dechelotte, Jildau Bouwman, Matthias Laudes, Tobias Pischon
Summary: Data access and integration are major challenges in research, with limited joint data analyses on nutrition and microbiomics in health and disease. By sharing and combining datasets from various countries, a better understanding of the relationship between food and gut microbiome in health and disease can be achieved.
Article
Oncology
Renaud Parment, Martine Dubois, Laurence Desrues, Alexandre Mutel, Kleouforo-Paul Dembele, Nicolas Belin, Laure Tron, Charlene Guerin, Moise Coeffier, Vincent Compere, Celine Feger, Florence Joly, Pascal Hilber, David Ribet, Helene Castel
Summary: Qiseng (R) has a preventive effect on fatigue and cognitive impairment caused by chemotherapy. It also regulates gut microbiota and inhibits inflammation.
Article
Gastroenterology & Hepatology
Chaima Ezzine, Lea Loison, Nadine Montbrion, Christine Bole-Feysot, Pierre Dechelotte, Moise Coeffier, David Ribet
Summary: Short chain fatty acids and branched chain fatty acids produced by the gut microbiota can increase protein SUMOylation, thus affecting intestinal physiology and reducing inflammation.
Article
Gastroenterology & Hepatology
Robin Louail, Franklin Florin, Sophie Bernard, Jean-Baptiste Michaud, Jonathan Breton, Najate Achamrah, Marie-Pierre Tavolacci, Moise Coeffier, David Ribet
Summary: Coagulase negative staphylococci (CoNS) are a diverse group of bacteria that colonize human epithelia. Staphylococcus warneri, previously considered nonpathogenic, is found to be a natural member of the gut microbiota in humans and mice. It is capable of invading human cells, especially intestinal epithelial cells, using actin-dependent mechanisms. Unlike true pathogens, S. warneri does not replicate within intestinal cells or resist killing by macrophages. These findings suggest that nonpathogenic bacteria from the gut microbiota can actively invade intestinal cells and potentially impact intestinal physiology.
Article
Microbiology
Pauline Tirelle, Jonathan Breton, Gaetan Riou, Pierre Dechelotte, Moise Coeffier, David Ribet
