Article
Biochemistry & Molecular Biology
Edward J. Calabrese
Summary: This paper provides a thorough assessment of hormetic dose responses in embryonic stem cells, suggesting that this response may serve as a fundamental and highly conserved evolutionary strategy.
CHEMICO-BIOLOGICAL INTERACTIONS
(2022)
Review
Biochemistry & Molecular Biology
Yahong Wu, Weiwei Zhang
Summary: Pluripotent stem cells, including ESCs and iPSCs, are crucial for studying early development and treating diseases, with ubiquitination playing a key role in pluripotency regulation, particularly through the actions of E3 ligases.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Engineering, Biomedical
Tingting Gao, Xiyuan Zhao, Jie Hao, Yao Tian, Huike Ma, Wenjing Liu, Bin An, Faguo Sun, Shasha Liu, Baojie Guo, Shuaishuai Niu, Zhongwen Li, Chenxin Wang, Yukai Wang, Guihai Feng, Liu Wang, Wei Li, Jun Wu, Meijin Guo, Qi Zhou, Qi Gu
Summary: In this study, a new microcarrier with hyaluronic acid (HA) as the core material was developed for scalable serum-free suspension culture of hESC-derived MSCs. The optimal microcarriers, with a coating collagen concentration of 100 μg/mL or concave-structured surface (cHAMCs), allowed for the expansion of IMRCs within six days with a high yield. The harvested cells showed high viability, immunomodulatory and regenerative therapeutic promise, as well as increased secretion of extracellular matrix (ECM).
MATERIALS TODAY BIO
(2023)
Article
Developmental Biology
Andrea L. Bredemeyer, Junedh M. Amrute, Andrew L. Koenig, Rachel A. Idol, Li He, Stephanie A. Luff, Carissa Dege, Jamison M. Leid, Joel D. Schilling, J. Travis Hinson, Mary C. Dinauer, Christopher M. Sturgeon, Kory J. Lavine
Summary: Tissue-resident macrophages play a crucial role in organ homeostasis, tissue repair, remodeling, and regeneration. Researchers have developed a system to generate macrophages that resemble tissue-resident or monocyte-derived subsets, allowing for the study of their function and potential applications in tissue engineering and regenerative medicine.
Article
Cell Biology
Chenze Xu, Ali Mohsin, Yanxia Luo, Lili Xie, Yan Peng, Qizheng Wang, Waqas Ahmed, Haifeng Hang, Yingping Zhuang, Meijin Guo
Summary: In this study, non-genetically modified Sertoli-like cells were successfully induced from mouse ESCs using defined protein factors, showing similar characteristics and functionality to natural Sertoli cells. However, the efficiency of the non-GM method was still lower compared to lentiviral transduction. This work lays a foundation for the future production of non-GM Sertoli-like cells for clinical applications and fundamental theory research.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Nanoscience & Nanotechnology
Baojie Guo, Yongchao Duan, Zhongwen Li, Yao Tian, Xuedi Cheng, Chunxu Liang, Wenjing Liu, Bin An, Wumei Wei, Tingting Gao, Shasha Liu, Xiyuan Zhao, Shuaishuai Niu, Chenxin Wang, Yukai Wang, Liu Wang, Guihai Feng, Wei Li, Jie Hao, Qi Gu, Qi Zhou, Jun Wu
Summary: The researchers developed a 21-day long-term serum-free culture system and successfully created cell sheets (CSs) with human embryonic stem cell-derived immunity-and-matrix-regulatory cells (IMRCs) that have stronger mechanical properties and greater extracellular matrix secretion capacity. These IMRC-CSs were effective in promoting corneal chemical injury repair and could be transformed into injectable temperature-sensitive hydrogels for uterine adhesion repair through a decellularization process.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Article
Chemistry, Multidisciplinary
Yingjie Hang, Xiaoliang Ma, Chunxiao Liu, Siyuan Li, Sixuan Zhang, Ruyan Feng, Qianwen Shang, Qi Liu, Zhaozhao Ding, Xiaoyi Zhang, Liyin Yu, Qiang Lu, Changshun Shao, Hong Chen, Yufang Shi, Jiuyang He, David L. Kaplan
Summary: The development of anisotropic silk protein nanofiber-based hydrogels enables mouse embryonic stem cells to maintain stemness in vitro without the need for leukemia inhibitory factor and mouse embryonic fibroblasts, resulting in superior pluripotency and genetic stability. The hydrogels establish an improved dynamic niche that stimulates autocrine factor secretion and maintains pluripotency and propagation of ESCs, offering a simple and cost-effective option for expansion and utility of ESCs in research and therapy, while providing insights into early mammalian embryogenesis.
Article
Biology
James Z. J. Kwan, Thomas F. Nguyen, Anuli C. Uzozie, Marek A. Budzynski, Jieying Cui, Joseph M. C. Lee, Filip Van Petegem, Philipp F. Lange, Sheila S. Teves
Summary: Depletion of TBP in mouse embryonic stem cells does not affect ongoing Pol II transcription, but severely impairs Pol III initiation. However, Pol II transcriptional induction still occurs normally upon TBP depletion. This TBP-independent transcription mechanism is not due to functional redundancy with TRF2, but rather the robustness of the TFIID complex and the Pol II machinery.
Article
Cell & Tissue Engineering
Miao Zhang, Xin Zhao, Xiao Feng, Xiao Hu, Xuan Zhao, Wange Lu, Xinyi Lu
Summary: This study reveals the distinct roles of HIRA complex members in controlling retrotransposons and cell fate conversion in embryonic stem cells.
STEM CELL RESEARCH & THERAPY
(2022)
Review
Biochemistry & Molecular Biology
Yong-Jin Kim, Amin Tamadon, Yoon-Young Kim, Byeong-Cheol Kang, Seung-Yup Ku
Summary: This review focuses on the current knowledge of epigenetic regulation of cardiomyocyte proliferation and differentiation from embryonic and induced pluripotent stem cells through histone modification and microRNAs, the maintenance of pluripotency, and its alteration during cardiac lineage differentiation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Engineering, Biomedical
Chao Sun, Yinhua Dong, Jun Wei, Meng Cai, Dongfang Liang, Yongqing Fu, You Zhou, Yi Sui, Fangda Wu, Roman Mikhaylov, Hanlin Wang, Feifei Fan, Zhihua Xie, Mercedes Stringer, Zhiyong Yang, Zhenlin Wu, Liangfei Tian, Xin Yang
Summary: This study develops a highly effective acoustic stimulator working at -20 MHz to investigate the roles of acousto-mechanical stimuli in the differentiation of hESCs. Our results show that acoustic dose alters the expression of extracellular matrix and physiological activity-related genes, indicating that acoustic stimulation is an important tool in regulating the stem cells' differentiation processes in cell therapy.
ACTA BIOMATERIALIA
(2022)
Article
Cell & Tissue Engineering
Yikai Huang, Ting Su, Congcong Wang, Lixia Dong, Shuang Liu, Yaru Zhu, Kunying Hao, Yin Xia, Qing Jiang, Jinzhong Qin
Summary: The deficiency of retinoblastoma binding protein 4 (RBBP4) in embryonic stem cells (ESCs) leads to spontaneous differentiation into mesendodermal lineages, and shares common genomic targets with the core Polycomb complex. RBBP4 is crucial for targeting PRC2 to a subset of developmental genes, and its presence is closely related to the maintenance of the pluripotency regulatory network and repression of mesendoderm lineages.
Article
Biochemistry & Molecular Biology
Guohua Meng, Andrea Lauria, Mara Maldotti, Francesca Anselmi, Isabelle Laurence Polignano, Stefania Rapelli, Daniela Donna, Salvatore Oliviero
Summary: Smad7 functions as a general transcription factor in ESCs, regulating both TGF-beta unrelated genes and key stemness regulators. Loss of Smad7 results in the downregulation of stemness master regulators and the upregulation of developmental genes, leading to loss of the stem phenotype. Integrative analysis reveals that Smad7 co-occupies promoters of highly expressed key stemness regulators by binding to a specific consensus response element.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Oncology
Borja Sese, Sandra Iniguez-Munoz, Miquel Ensenyat-Mendez, Pere Llinas-Arias, Guillem Ramis, Javier I. J. Orozco, Silvia Fernandez de Mattos, Priam Villalonga, Diego M. Marzese
Summary: This study identifies a subpopulation of glioma stem cells (GSCs), known as core-GSCs (c-GSCs), in glioblastoma (GBM) patients that have a similar profile to embryonic stem cells (ESCs) and downregulated immune-associated pathways. The development of an induced c-GSC (ic-GSC) cellular model provides insights into the immune evasion mechanisms in GBM and potential targets for improving immunotherapy treatments.
Article
Cell & Tissue Engineering
Hye Ji Moon, Na Yeon Lee, Eun Kyoung Do, Seo Yul Lee, Gyu Tae Park, Jae Kyong Lim, Jeong Kon Seo, Jae Ho Kim
Summary: In this study, Kap1 was identified as a novel Lin28A-binding protein, which regulates the stability of Lin28A through modulating the Trim71-mediated ubiquitination and degradation of Lin28A, thus playing a crucial role in the regulation of ESC self-renewal and pluripotency.
Article
Developmental Biology
Astrid Houben, Daniela Kostanova-Poliakova, Martina Weissenboeck, Julian Graf, Stefan Teufel, Klaus von der Mark, Christine Hartmann
Article
Cell Biology
Corinna Wehmeyer, Svetlana Frank, Denise Beckmann, Martin Boettcher, Christoph Cromme, Ulrich Koenig, Michelle Fennen, Annelena Held, Peter Paruzel, Christine Hartmann, Athanasios Stratis, Adelheid Korb-Pap, Thomas Kamradt, Ina Kramer, Wim van den Berg, Michaela Kneissel, Thomas Pap, Berno Dankbar
SCIENCE TRANSLATIONAL MEDICINE
(2016)
Article
Endocrinology & Metabolism
Glenda Comai, Agnes Boutet, Kristina Tanneberger, Filippo Massa, Ana-Sofia Rocha, Aurelie Charlet, Clara Panzolini, Fariba Jian Motamedi, Robert Brommage, Wolfgang Hans, Thomas Funck-Brentano, Martin Hrabe de Angelis, Christine Hartmann, Martine Cohen-Solal, Juergen Behrens, Andreas Schedl
JOURNAL OF BONE AND MINERAL RESEARCH
(2018)
Article
Cell Biology
Martina Weissenboeck, Richard Latham, Michiru Nishita, Lena Ingeborg Wolff, Hsin-Yi Henry Ho, Yasuhiro Minami, Christine Hartmann
Review
Endocrinology & Metabolism
Lena Ingeborg Wolff, Christine Hartmann
CURRENT OSTEOPOROSIS REPORTS
(2019)
Article
Cell Biology
Ulrich Koenig, Horst Robenek, Caterina Barresi, Marlene Brandstetter, Guenter P. Resch, Marion Groeger, Thomas Pap, Christine Hartman
Retraction
Cell Biology
Tahereh Javaheri, Zahra Kazemi, Jan Pencik, Ha T. T. Pham, Maximilian Kauer, Rahil Noorizadeh, Barbara Sax, Harini Nivarthi, Michaela Schlederer, Barbara Maurer, Maximillian Hofbauer, Dave N. T. Aryee, Marc Wiedner, EleniM. Tomazou, Malcolm Logan, Christine Hartmann, Jan P. Tuckermann, Lukas Kenner, Mario Mikula, Helmut Dolznig, Aykut Uren, Guenther H. Richter, Florian Grebien, Heinrich Kovar, Richard Moriggl
CELL DEATH & DISEASE
(2019)
Article
Cell Biology
Kazuhiko Matsuoka, Latifa Bakiri, Lena Wolff, Markus Linder, Amanda Mikels-Vigdal, Ana Patino-Garcia, Fernando Lecanda, Christine Hartmann, Maria Sibilia, Erwin F. Wagner
Article
Endocrinology & Metabolism
Zhijia Tan, Mingpeng Kong, Songjia Wen, Kwok Yeung Tsang, Ben Niu, Christine Hartmann, Danny Chan, Chi-Chung Hui, Kathryn S. E. Cheah
JOURNAL OF BONE AND MINERAL RESEARCH
(2020)
Article
Multidisciplinary Sciences
Meng Zhang, Yiwei Lai, Vladislav Krupalnik, Pengcheng Guo, Xiangpeng Guo, Jianguo Zhou, Yan Xu, Zhijun Yu, Longqi Liu, Ao Jiang, Wenjuan Li, Gang Ma, Na Li, Xiuling Fu, Yuan Lv, Mengling Jiang, Muqddas Tariq, Shahzina Kanwal, Hao Liu, Xueting Xu, Hui Zhang, Yinghua Huang, Lulu Wang, Shuhan Chen, Isaac A. Babarinde, Zhiwei Luo, Dongye Wang, Tiantian Zhou, Carl Ward, Minghui He, David P. Ibanez, Yunpan Li, Jiajian Zhou, Jie Yuan, Yayan Feng, Karthik Arumugam, Umberto Di Vicino, Xichen Bao, Guangming Wu, Axel Schambach, Huating Wang, Hao Sun, Fei Gao, Baoming Qin, Andrew P. Hutchins, Bradley W. Doble, Christine Hartmann, Maria Pia Cosma, Yan Qin, Guo-Liang Xu, Runsheng Chen, Giacomo Volpe, Liang Chen, Jacob H. Hanna, Miguel A. Esteban
Article
Cell Biology
Stefan Teufel, Petra Koeckemann, Christine Fabritius, Lena Wolff, Jessica Bertrand, Thomas Pap, Christine Hartmann
Summary: Agonists and antagonists of the canonical Wnt signaling pathway have an impact on rheumatoid arthritis, primarily modifying bone loss and soft-tissue inflammation. WNT9a levels are elevated in the pannus tissue of RA patients, suggesting a specific role in TNF-triggered RA.
CELL DEATH & DISEASE
(2021)
Article
Endocrinology & Metabolism
Stefan Teufel, Lena Wolff, Ulrich Koenig, Akio Kobayashi, Richard Behringer, Christine Hartmann
Summary: Osteoarthritis (OA) is a common degenerative joint disease with a complex and not fully understood etiology. Recent research has shown the involvement of the Wnt signaling pathway in OA. A study identified the chromosomal location on chromosome 1 linked to the Wnt3a-Wnt9a gene locus as the most significant locus associated with thumb osteoarthritis. The conditional loss of Wnt9a and Wnt4 in mice was found to predispose them to develop spontaneous OA-like changes and increased disease severity.
JOURNAL OF BONE AND MINERAL RESEARCH
(2022)
Article
Endocrinology & Metabolism
Benjamin Tosun, Lena Ingeborg Wolff, Astrid Houben, Stephen Nutt, Christine Hartmann
Summary: Osteoclasts play an important role in bone marrow cavity formation, while macrophages appear to have no major role. Studies have shown that mice lacking osteoclasts or macrophages display a delay in bone marrow cavity formation and a lengthening of the zone of hypertrophic chondrocytes during embryonic development.
JOURNAL OF BONE AND MINERAL RESEARCH
(2022)
Article
Cell Biology
Laura Lleras-Forero, Elis Newham, Stefan Teufel, Koichi Kawakami, Christine Hartmann, Chrissy L. Hammond, Robert D. Knight, Stefan Schulte-Merker
Article
Cell Biology
Chandra S. Amara, Christine Fabritius, Astrid Houben, Lena I. Wolff, Christine Hartmann
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2017)
