Recombinant messenger RNA technology and its application in cancer immunotherapy, transcript replacement therapies, pluripotent stem cell induction, and beyond

Immunomonitoring of a phase I/II study of AGS-003, a dendritic cell immunotherapeutic, as first-line treatment for metastatic renal cell carcinoma.

(2017) D. Healey et al.   JOURNAL OF CLINICAL ONCOLOGY

Final analysis of a phase I/IIa study with CV9103, an intradermally administered prostate cancer immunotherapy based on self-adjuvanted mRNA.

(2017) H. Kübler et al.   JOURNAL OF CLINICAL ONCOLOGY

Synthetic Chemically Modified mRNA-Based Delivery of Cytoprotective Factor Promotes Early Cardiomyocyte Survival Post-Acute Myocardial Infarction

(2015) Chien-Ling Huang et al.   MOLECULAR PHARMACEUTICS

Induction of Cytomegalovirus-Specific T Cell Responses in Healthy Volunteers and Allogeneic Stem Cell Recipients Using Vaccination With Messenger RNA–Transfected Dendritic Cells

(2015) Amaryllis H. Van Craenenbroeck et al.   TRANSPLANTATION

Long Overall Survival After Dendritic Cell Vaccination in Metastatic Uveal Melanoma Patients

(2014) Kalijn F. Bol et al.   AMERICAN JOURNAL OF OPHTHALMOLOGY

Phase Ib study evaluating a self-adjuvanted mRNA cancer vaccine (RNActive®) combined with local radiation as consolidation and maintenance treatment for patients with stage IV non-small cell lung cancer

(2014) Martin Sebastian et al.   BMC CANCER

Long-term clinical outcome of melanoma patients treated with messenger RNA-electroporated dendritic cell therapy following complete resection of metastases

(2014) Sofie Wilgenhof et al.   CANCER IMMUNOLOGY IMMUNOTHERAPY

Potent Immune Responses in Rhesus Macaques Induced by Nonviral Delivery of a Self-amplifying RNA Vaccine Expressing HIV Type 1 Envelope With a Cationic Nanoemulsion

(2014) W. M. Bogers et al.   JOURNAL OF INFECTIOUS DISEASES

Adoptive immunotherapy with MUC1-mRNA transfected dendritic cells and cytotoxic lymphocytes plus gemcitabine for unresectable pancreatic cancer

(2014) Yoshitaro Shindo et al.   Journal of Translational Medicine

CRISPR-Cas systems for editing, regulating and targeting genomes

(2014) Jeffry D Sander et al.   NATURE BIOTECHNOLOGY

Linking T-cell receptor sequence to functional phenotype at the single-cell level

(2014) Arnold Han et al.   NATURE BIOTECHNOLOGY

Modeling the mitochondrial cardiomyopathy of Barth syndrome with induced pluripotent stem cell and heart-on-chip technologies

(2014) Gang Wang et al.   NATURE MEDICINE

Rapid and Efficient Conversion of Integration-Free Human Induced Pluripotent Stem Cells to GMP-Grade Culture Conditions

(2014) Jens Durruthy-Durruthy et al.   PLoS One

Direct Reprogramming of Human Fibroblasts to Hepatocyte-Like Cells by Synthetic Modified mRNAs

(2014) Kamen P. Simeonov et al.   PLoS One

A Systemic Evaluation of Cardiac Differentiation from mRNA Reprogrammed Human Induced Pluripotent Stem Cells

(2014) Ashish Mehta et al.   PLoS One

Functional TCR Retrieval from Single Antigen-Specific Human T Cells Reveals Multiple Novel Epitopes

(2014) P. Simon et al.   Cancer Immunology Research

3D In Vitro Model of a Functional Epidermal Permeability Barrier from Human Embryonic Stem Cells and Induced Pluripotent Stem Cells

(2014) Anastasia Petrova et al.   Stem Cell Reports

A phase IB study on intravenous synthetic mRNA electroporated dendritic cell immunotherapy in pretreated advanced melanoma patients

(2013) S. Wilgenhof et al.   ANNALS OF ONCOLOGY

Synthesis and biological validation of N7-(4-chlorophenoxyethyl) substituted dinucleotide cap analogs for mRNA translation

(2013) Anilkumar R. Kore et al.   BIOORGANIC & MEDICINAL CHEMISTRY

The synthesis of isopropylidene mRNA cap analogs modified with phosphorothioate moiety and their evaluation as promoters of mRNA translation

(2013) Marcin Warminski et al.   BIOORGANIC & MEDICINAL CHEMISTRY LETTERS

mRNA-engineered mesenchymal stem cells for targeted delivery of interleukin-10 to sites of inflammation

(2013) O. Levy et al.   BLOOD

Therapeutic vaccination against autologous cancer stem cells with mRNA-transfected dendritic cells in patients with glioblastoma

(2013) Einar Osland Vik-Mo et al.   CANCER IMMUNOLOGY IMMUNOTHERAPY

Immunogenicity of dendritic cells pulsed with MAGE3, Survivin and B-cell maturation antigen mRNA for vaccination of multiple myeloma patients

(2013) Willemijn Hobo et al.   CANCER IMMUNOLOGY IMMUNOTHERAPY

Driving vascular endothelial cell fate of human multipotent Isl1+ heart progenitors with VEGF modified mRNA

(2013) Kathy O Lui et al.   CELL RESEARCH

Human iPSC-Based Modeling of Late-Onset Disease via Progerin-Induced Aging

(2013) Justine D. Miller et al.   Cell Stem Cell

Efficient Generation of Human iPSCs by a Synthetic Self-Replicative RNA

(2013) Naohisa Yoshioka et al.   Cell Stem Cell

Roadmap to Cellular Reprogramming – Manipulating Transcriptional Networks with DNA, RNA, Proteins and Small Molecules

(2013) P. Worsdorfer et al.   CURRENT MOLECULAR MEDICINE

Melanoma immunotherapy using mature DCs expressing the constitutive proteasome

(2013) Jens Dannull et al.   JOURNAL OF CLINICAL INVESTIGATION

Modified Foxp3 mRNA protects against asthma through an IL-10–dependent mechanism

(2013) Lauren E. Mays et al.   JOURNAL OF CLINICAL INVESTIGATION

Ifit1 Inhibits Japanese Encephalitis Virus Replication through Binding to 5' Capped 2'-O Unmethylated RNA

(2013) T. Kimura et al.   JOURNAL OF VIROLOGY

mRNA transfection-based, feeder-free, induced pluripotent stem cells derived from adipose tissue of a 50-year-old patient

(2013) Boon Chin Heng et al.   METABOLIC ENGINEERING

Modified mRNA directs the fate of heart progenitor cells and induces vascular regeneration after myocardial infarction

(2013) Lior Zangi et al.   NATURE BIOTECHNOLOGY

Reprogramming human fibroblasts to pluripotency using modified mRNA

(2013) Pankaj K Mandal et al.   Nature Protocols

In Vivo Messenger RNA Introduction into the Central Nervous System Using Polyplex Nanomicelle

(2013) Satoshi Uchida et al.   PLoS One

Strong anion-exchange fast performance liquid chromatography as a versatile tool for preparation and purification of RNA produced by in vitro transcription

(2013) J. Koubek et al.   RNA

RNA-Based Tools for Nuclear Reprogramming and Lineage-Conversion: Towards Clinical Applications

(2013) Juan A. Bernal   Journal of Cardiovascular Translational Research

A novel, disruptive vaccination technology

(2013) Karl-Josef Kallen et al.   Human Vaccines & Immunotherapeutics

Sequestration by IFIT1 Impairs Translation of 2′O-unmethylated Capped RNA

(2013) Matthias Habjan et al.   PLoS Pathogens

Messenger RNA-based vaccines: progress, challenges, applications

(2013) Thomas Kramps et al.   Wiley Interdisciplinary Reviews-RNA

Characterization of CD8+T-Cell Responses in the Peripheral Blood and Skin Injection Sites of Melanoma Patients Treated with mRNA Electroporated Autologous Dendritic Cells (TriMixDC-MEL)

(2013) Daphné Benteyn et al.   Biomed Research International

Nonviral Methods for Inducing Pluripotency to Cells

(2013) Ryan O'Doherty et al.   Biomed Research International

Getting Personal with Neoantigen-Based Therapeutic Cancer Vaccines

(2013) N. Hacohen et al.   Cancer Immunology Research

Design of an Optimized Wilms’ Tumor 1 (WT1) mRNA Construct for Enhanced WT1 Expression and Improved Immunogenicity In Vitro and In Vivo

(2013) Daphné Benteyn et al.   Molecular Therapy-Nucleic Acids

The cytotoxic and immunogenic hurdles associated with non-viral mRNA-mediated reprogramming of human fibroblasts

(2012) Katharina Drews et al.   BIOMATERIALS

Synthesis and properties of mRNA cap analogs containing imidodiphosphate moiety—fairly mimicking natural cap structure, yet resistant to enzymatic hydrolysis

(2012) Anna M. Rydzik et al.   BIOORGANIC & MEDICINAL CHEMISTRY

Exploiting the Mutanome for Tumor Vaccination

(2012) J. C. Castle et al.   CANCER RESEARCH

Activation of Innate Immunity Is Required for Efficient Nuclear Reprogramming

(2012) Jieun Lee et al.   CELL

Vaccination with mRNA-Electroporated Dendritic Cells Induces Robust Tumor Antigen-Specific CD4+ and CD8+ T Cells Responses in Stage III and IV Melanoma Patients

(2012) E. H. J. G. Aarntzen et al.   CLINICAL CANCER RESEARCH

TALE nucleases: tailored genome engineering made easy

(2012) Claudio Mussolino et al.   CURRENT OPINION IN BIOTECHNOLOGY

Targeting Serous Epithelial Ovarian Cancer with Designer Zinc Finger Transcription Factors

(2012) Haydee Lara et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Evaluation of a microfluidics-based platform and slab electrophoresis for determination of size, integrity and quantification of in vitro transcribed RNA used as a component in therapeutic drug manufacturing

(2012) Jacoba Grietje Slagter-Jäger et al.   JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS

Mesothelin-Targeted Agents in Clinical Trials and in Preclinical Development

(2012) R. J. Kelly et al.   MOLECULAR CANCER THERAPEUTICS

UU/UA Dinucleotide Frequency Reduction in Coding Regions Results in Increased mRNA Stability and Protein Expression

(2012) Maher Al-Saif et al.   MOLECULAR THERAPY

Increased Erythropoiesis in Mice Injected With Submicrogram Quantities of Pseudouridine-containing mRNA Encoding Erythropoietin

(2012) Katalin Karikó et al.   MOLECULAR THERAPY

Systemic Delivery of Modified mRNA Encoding Herpes Simplex Virus 1 Thymidine Kinase for Targeted Cancer Gene Therapy

(2012) Yuhua Wang et al.   MOLECULAR THERAPY

Systematic discovery of structural elements governing stability of mammalian messenger RNAs

(2012) Hani Goodarzi et al.   NATURE

Protective efficacy of in vitro synthesized, specific mRNA vaccines against influenza A virus infection

(2012) Benjamin Petsch et al.   NATURE BIOTECHNOLOGY

Regulation of cytoplasmic mRNA decay

(2012) Daniel R. Schoenberg et al.   NATURE REVIEWS GENETICS

Characterization of Coding Synonymous and Non-Synonymous Variants in ADAMTS13 Using Ex Vivo and In Silico Approaches

(2012) Nathan C. Edwards et al.   PLoS One

Nonviral delivery of self-amplifying RNA vaccines

(2012) A. J. Geall et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Speeding with control: codon usage, tRNAs, and ribosomes

(2012) Eva Maria Novoa et al.   TRENDS IN GENETICS

Feeder-Free Derivation of Human Induced Pluripotent Stem Cells with Messenger RNA

(2012) Luigi Warren et al.   Scientific Reports

mRNA-based dendritic cell vaccination induces potent antiviral T-cell responses in HIV-1-infected patients

(2011) Ellen Van Gulck et al.   AIDS

Activation of pluripotency-associated genes in mouse embryonic fibroblasts by non-viral transfection with in vitro-derived mRNAs encoding Oct4, Sox2, Klf4 and cMyc

(2011) Geertrui Tavernier et al.   BIOMATERIALS

Codon usage: Nature's roadmap to expression and folding of proteins

(2011) Evelina Angov   Biotechnology Journal

Intravenous and intradermal TriMix-dendritic cell therapy results in a broad T-cell response and durable tumor response in a chemorefractory stage IV-M1c melanoma patient

(2011) An M. T. Van Nuffel et al.   CANCER IMMUNOLOGY IMMUNOTHERAPY

hTERT mRNA dendritic cell vaccination: complete response in a pancreatic cancer patient associated with response against several hTERT epitopes

(2011) Else M. Inderberg Suso et al.   CANCER IMMUNOLOGY IMMUNOTHERAPY

A phase I/IIa immunotherapy trial of HIV-1-infected patients with Tat, Rev and Nef expressing dendritic cells followed by treatment interruption

(2011) Sabine D. Allard et al.   CLINICAL IMMUNOLOGY

Zinc-Finger Nucleases: A Panoramic View

(2011) Dana Carroll   CURRENT GENE THERAPY

Prospects and Limitations of T Cell Receptor Gene Therapy

(2011) Annelies Jorritsma et al.   CURRENT GENE THERAPY

Zinc-Finger Nuclease Based Genome Surgery: Its All About Specificity

(2011) Eva-Maria Handel et al.   CURRENT GENE THERAPY

Coordinate regulation of mRNA decay networks by GU-rich elements and CELF1

(2011) Irina Vlasova-St. Louis et al.   CURRENT OPINION IN GENETICS & DEVELOPMENT

Tumor vaccination using messenger RNA: prospects of a future therapy

(2011) Sebastian Kreiter et al.   CURRENT OPINION IN IMMUNOLOGY

mRNA vaccination as a safe approach for specific protection from type I allergy

(2011) Richard Weiss et al.   Expert Review of Vaccines

Selective uptake of naked vaccine RNA by dendritic cells is driven by macropinocytosis and abrogated upon DC maturation

(2011) M Diken et al.   GENE THERAPY

Treatment of Advanced Leukemia in Mice with mRNA Engineered T Cells

(2011) David M. Barrett et al.   HUMAN GENE THERAPY

Tumor Regression in Patients With Metastatic Synovial Cell Sarcoma and Melanoma Using Genetically Engineered Lymphocytes Reactive With NY-ESO-1

(2011) Paul F. Robbins et al.   JOURNAL OF CLINICAL ONCOLOGY

Therapeutic Vaccination With an Autologous mRNA Electroporated Dendritic Cell Vaccine in Patients With Advanced Melanoma

(2011) Sofie Wilgenhof et al.   JOURNAL OF IMMUNOTHERAPY

In Vitroandin VivomRNA Delivery Using Lipid-Enveloped pH-Responsive Polymer Nanoparticles

(2011) Xingfang Su et al.   MOLECULAR PHARMACEUTICS

DARPins: An Efficient Targeting Domain for Lentiviral Vectors

(2011) Robert C Münch et al.   MOLECULAR THERAPY

Global quantification of mammalian gene expression control

(2011) Björn Schwanhäusser et al.   NATURE

Knockout rats generated by embryo microinjection of TALENs

(2011) Laurent Tesson et al.   NATURE BIOTECHNOLOGY

Expression of therapeutic proteins after delivery of chemically modified mRNA in mice

(2011) Michael S D Kormann et al.   NATURE BIOTECHNOLOGY

Ribose 2′-O-methylation provides a molecular signature for the distinction of self and non-self mRNA dependent on the RNA sensor Mda5

(2011) Roland Züst et al.   NATURE IMMUNOLOGY

Structural and biochemical studies of the 5′→3′ exoribonuclease Xrn1

(2011) Jeong Ho Chang et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Generating the optimal mRNA for therapy: HPLC purification eliminates immune activation and improves translation of nucleoside-modified, protein-encoding mRNA

(2011) Katalin Karikó et al.   NUCLEIC ACIDS RESEARCH

Avoiding cytotoxicity of transposases by dose-controlled mRNA delivery

(2011) Melanie Galla et al.   NUCLEIC ACIDS RESEARCH

Multiparameter RNA and Codon Optimization: A Standardized Tool to Assess and Enhance Autologous Mammalian Gene Expression

(2011) Stephan Fath et al.   PLoS One

Protein expression from exogenous mRNA: Uptake by receptor-mediated endocytosis and trafficking via the lysosomal pathway

(2011) Christina Lorenz et al.   RNA Biology

Determinants of intracellular RNA pharmacokinetics: Implications for RNA-based immunotherapeutics

(2011) Andreas N. Kuhn et al.   RNA Biology

The eukaryotic RNA exosome: Same scaffold but variable catalytic subunits

(2011) Søren Lykke-Andersen et al.   RNA Biology

Translation, stability, and resistance to decapping of mRNAs containing caps substituted in the triphosphate chain with BH3, Se, and NH

(2011) W. Su et al.   RNA

T Cells with Chimeric Antigen Receptors Have Potent Antitumor Effects and Can Establish Memory in Patients with Advanced Leukemia

(2011) M. Kalos et al.   Science Translational Medicine

Treating cancer with genetically engineered T cells

(2011) Tristen S. Park et al.   TRENDS IN BIOTECHNOLOGY

Mechanisms of endonuclease-mediated mRNA decay

(2011) Daniel R. Schoenberg   Wiley Interdisciplinary Reviews-RNA

Reprogramming of human fibroblasts to pluripotent stem cells using mRNA of four transcription factors

(2010) Eduard Yakubov et al.   BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS

A high throughput microelectroporation device to introduce a chimeric antigen receptor to redirect the specificity of human T cells

(2010) Yoonsu Choi et al.   BIOMEDICAL MICRODEVICES

Synthesis and evaluation of 2′-O-allyl substituted dinucleotide cap analog for mRNA translation

(2010) Anilkumar R. Kore et al.   BIOORGANIC & MEDICINAL CHEMISTRY

Direct and indirect antitumor effects by human peripheral blood lymphocytes expressing both chimeric immune receptor and interleukin-2 in ovarian cancer xenograft model

(2010) J M Lee et al.   CANCER GENE THERAPY

Intranodal Vaccination with Naked Antigen-Encoding RNA Elicits Potent Prophylactic and Therapeutic Antitumoral Immunity

(2010) S. Kreiter et al.   CANCER RESEARCH

Multiple Injections of Electroporated Autologous T Cells Expressing a Chimeric Antigen Receptor Mediate Regression of Human Disseminated Tumor

(2010) Y. Zhao et al.   CANCER RESEARCH

Highly Efficient Reprogramming to Pluripotency and Directed Differentiation of Human Cells with Synthetic Modified mRNA

(2010) Luigi Warren et al.   Cell Stem Cell

Building Better Chimeric Antigen Receptors for Adoptive T Cell Therapy

(2010) John S. Bridgeman et al.   CURRENT GENE THERAPY

Phosphorothioate cap analogs increase stability and translational efficiency of RNA vaccines in immature dendritic cells and induce superior immune responses in vivo

(2010) A N Kuhn et al.   GENE THERAPY

Targeted mutagenesis in the silkworm Bombyx mori using zinc finger nuclease mRNA injection

(2010) Yoko Takasu et al.   INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY

Lipid-mediated delivery of RNA is more efficient than delivery of DNA in non-dividing cells

(2010) S. Zou et al.   INTERNATIONAL JOURNAL OF PHARMACEUTICS

Development of Defective and Persistent Sendai Virus Vector

(2010) Ken Nishimura et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

mRNA as gene therapeutic: How to control protein expression

(2010) Geertrui Tavernier et al.   JOURNAL OF CONTROLLED RELEASE

Selective gene delivery in dendritic cells with mannosylated and histidylated lipopolyplexes

(2010) Federico Perche et al.   JOURNAL OF DRUG TARGETING

A Short Pulse of IL-4 Delivered by DCs Electroporated With Modified mRNA Can Both Prevent and Treat Autoimmune Diabetes in NOD Mice

(2010) Rémi J Creusot et al.   MOLECULAR THERAPY

Case Report of a Serious Adverse Event Following the Administration of T Cells Transduced With a Chimeric Antigen Receptor Recognizing ERBB2

(2010) Richard A Morgan et al.   MOLECULAR THERAPY

T Cells Targeting Carcinoembryonic Antigen Can Mediate Regression of Metastatic Colorectal Cancer but Induce Severe Transient Colitis

(2010) Maria R Parkhurst et al.   MOLECULAR THERAPY

Intradermal Vaccinations With RNA Coding for TAA Generate CD8+ and CD4+ Immune Responses and Induce Clinical Benefit in Vaccinated Patients

(2010) Susanne M Rittig et al.   MOLECULAR THERAPY

2′-O methylation of the viral mRNA cap evades host restriction by IFIT family members

(2010) Stephane Daffis et al.   NATURE

Synonymous but not the same: the causes and consequences of codon bias

(2010) Joshua B. Plotkin et al.   NATURE REVIEWS GENETICS

Structural and functional insights into 5′-ppp RNA pattern recognition by the innate immune receptor RIG-I

(2010) Yanli Wang et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Towards mRNA with superior translational activity: synthesis and properties of ARCA tetraphosphates with single phosphorothioate modifications

(2010) Malwina Strenkowska et al.   NEW JOURNAL OF CHEMISTRY

Synthesis and biological activity of artificial mRNA prepared with novel phosphorylating reagents

(2010) Seigo Nagata et al.   NUCLEIC ACIDS RESEARCH

Innate Immune Suppression Enables Frequent Transfection with RNA Encoding Reprogramming Proteins

(2010) Matthew Angel et al.   PLoS One

Activation of Pluripotency Genes in Human Fibroblast Cells by a Novel mRNA Based Approach

(2010) Jordan R. Plews et al.   PLoS One

Induction of complete and molecular remissions in acute myeloid leukemia by Wilms' tumor 1 antigen-targeted dendritic cell vaccination

(2010) V. F. Van Tendeloo et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Rapid, nondenaturing RNA purification using weak anion-exchange fast performance liquid chromatography

(2010) L. E. Easton et al.   RNA

Structural and functional insights into eukaryotic mRNA decapping

(2010) Sharon H. M. Ling et al.   Wiley Interdisciplinary Reviews-RNA

Regulation of mRNA decapping

(2010) You Li et al.   Wiley Interdisciplinary Reviews-RNA

Mechanisms of deadenylation-dependent decay

(2010) Chyi-Ying A. Chen et al.   Wiley Interdisciplinary Reviews-RNA

Sequential analysis of RNA synthesis by microchip electrophoresis

(2009) Yoshihiro Umemoto et al.   ANALYTICAL BIOCHEMISTRY

Gene therapy with human and mouse T-cell receptors mediates cancer regression and targets normal tissues expressing cognate antigen

(2009) L. A. Johnson et al.   BLOOD

Chemical vectors for gene delivery: a current review on polymers, peptides and lipids containing histidine or imidazole as nucleic acids carriers

(2009) Patrick Midoux et al.   BRITISH JOURNAL OF PHARMACOLOGY

Expression of chimeric antigen receptors in natural killer cells with a regulatory-compliant non-viral method

(2009) L Li et al.   CANCER GENE THERAPY

In situ Expression of Tumor Antigens by Messenger RNA-Electroporated Dendritic Cells in Lymph Nodes of Melanoma Patients

(2009) D. H. Schuurhuis et al.   CANCER RESEARCH

Regulation of Translation Initiation in Eukaryotes: Mechanisms and Biological Targets

(2009) Nahum Sonenberg et al.   CELL

Widespread Shortening of 3′UTRs by Alternative Cleavage and Polyadenylation Activates Oncogenes in Cancer Cells

(2009) Christine Mayr et al.   CELL

Single-Step Antigen Loading and Activation of Dendritic Cells by mRNA Electroporation for the Purpose of Therapeutic Vaccination in Melanoma Patients

(2009) A. Bonehill et al.   CLINICAL CANCER RESEARCH

Immunologic activity and safety of autologous HIV RNA-electroporated dendritic cells in HIV-1 infected patients receiving antiretroviral therapy

(2009) Jean-Pierre Routy et al.   CLINICAL IMMUNOLOGY

Immunotherapy of Human Cancers Using Gene Modified T Lymphocytes

(2009) Juan Vera et al.   CURRENT GENE THERAPY

Clinical-grade manufacturing of autologous mature mRNA-electroporated dendritic cells and safety testing in acute myeloid leukemia patients in a phase I dose-escalation clinical trial

(2009) Ann Van Driessche et al.   CYTOTHERAPY

Chimeric Receptor mRNA Transfection as a Tool to Generate Antineoplastic Lymphocytes

(2009) Peter M. Rabinovich et al.   HUMAN GENE THERAPY

Direct Injection of Protamine-protected mRNA: Results of a Phase 1/2 Vaccination Trial in Metastatic Melanoma Patients

(2009) Benjamin Weide et al.   JOURNAL OF IMMUNOTHERAPY

Locked Nucleic Acid (LNA)-Modified Dinucleotide mRNA Cap Analogue: Synthesis, Enzymatic Incorporation, and Utilization

(2009) Anilkumar R. Kore et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Synthetic dinucleotide mRNA cap analogs with tetraphosphate 5′,5′ bridge containing methylenebis(phosphonate) modification

(2009) Anna Maria Rydzik et al.   ORGANIC & BIOMOLECULAR CHEMISTRY

Potential role of phenotypic mutations in the evolution of protein expression and stability

(2009) M. Goldsmith et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Knockout Rats via Embryo Microinjection of Zinc-Finger Nucleases

(2009) Aron M. Geurts et al.   SCIENCE

Lentiviral vectors with measles virus glycoproteins – dream team for gene transfer?

(2009) Christian J. Buchholz et al.   TRENDS IN BIOTECHNOLOGY

Alphaviruses in Gene Therapy

(2009) Kenneth Lundstrom   Viruses-Basel

Synthesis and application of a new 2′,3′-isopropylidene guanosine substituted cap analog

(2008) Anilkumar R. Kore et al.   BIOORGANIC & MEDICINAL CHEMISTRY LETTERS

Adoptive immunotherapy for indolent non-Hodgkin lymphoma and mantle cell lymphoma using genetically modified autologous CD20-specific T cells

(2008) B. G. Till et al.   BLOOD

Ectopic expression of a truncated CD40L protein from synthetic post-transcriptionally capped RNA in dendritic cells induces high levels of IL-12 secretion

(2008) Irina Y Tcherepanova et al.   BMC MOLECULAR BIOLOGY

Adoptive immunotherapy using human peripheral blood lymphocytes transferred with RNA encoding Her-2/neu-specific chimeric immune receptor in ovarian cancer xenograft model

(2008) S H Yoon et al.   CANCER GENE THERAPY

Modified tumour antigen-encoding mRNA facilitates the analysis of naturally occurring and vaccine-induced CD4 and CD8 T cells in cancer patients

(2008) Ashley J. Knights et al.   CANCER IMMUNOLOGY IMMUNOTHERAPY

Enzymatic Incorporation of Emissive Pyrimidine Ribonucleotides

(2008) Seergazhi G. Srivatsan et al.   Chemistry-An Asian Journal

Activated B cells modified by electroporation of multiple mRNAs encoding immune stimulatory molecules are comparable to mature dendritic cells in inducingin vitroantigen-specific T-cell responses

(2008) Jaewoo Lee et al.   IMMUNOLOGY

Length-dependent recognition of double-stranded ribonucleic acids by retinoic acid–inducible gene-I and melanoma differentiation–associated gene 5

(2008) Hiroki Kato et al.   JOURNAL OF EXPERIMENTAL MEDICINE

Bax mRNA therapy using cationic liposomes for human malignant melanoma

(2008) Kenya Okumura et al.   JOURNAL OF GENE MEDICINE

Results of the First Phase I/II Clinical Vaccination Trial With Direct Injection of mRNA

(2008) Benjamin Weide et al.   JOURNAL OF IMMUNOTHERAPY

Polymerase Chain Reaction and Transcription Using Locked Nucleic Acid Nucleotide Triphosphates

(2008) Rakesh N. Veedu et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Toll-Like Receptor 3 Has a Protective Role against West Nile Virus Infection

(2008) S. Daffis et al.   JOURNAL OF VIROLOGY

Incorporation of Pseudouridine Into mRNA Yields Superior Nonimmunogenic Vector With Increased Translational Capacity and Biological Stability

(2008) Katalin Karikó et al.   MOLECULAR THERAPY

Sequence- and target-independent angiogenesis suppression by siRNA via TLR3

(2008) Mark E. Kleinman et al.   NATURE

Heritable targeted gene disruption in zebrafish using designed zinc-finger nucleases

(2008) Yannick Doyon et al.   NATURE BIOTECHNOLOGY

Mechanisms of post-transcriptional regulation by microRNAs: are the answers in sight?

(2008) Witold Filipowicz et al.   NATURE REVIEWS GENETICS

Efficient gene targeting in Drosophila by direct embryo injection with zinc-finger nucleases

(2008) K. J. Beumer et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Structural Basis of Toll-Like Receptor 3 Signaling with Double-Stranded RNA

(2008) L. Liu et al.   SCIENCE