Review
Pharmacology & Pharmacy
Jiaxing Li, Xiao Wang, Jiayu Ding, Yasheng Zhu, Wenjian Min, Wenbing Kuang, Kai Yuan, Chengliang Sun, Peng Yang
Summary: Hematopoietic stem cell transplantation is the only curative therapy for many diseases. Umbilical cord blood (UCB) source of HSCs has advantages over bone marrow, but has limited dose. Small molecule-mediated HSC expansion has shown promise in overcoming this limitation.
ACTA PHARMACEUTICA SINICA B
(2022)
Article
Biophysics
Alice Pievani, Valentina Granata, Giacomo Desantis, Laura Antolini, Sara Ornaghi, Antonio Galleu, Andrea Biondi, Bernhard Gentner, Francesco Dazzi, Marta Serafini
Summary: Monocytes are effective at improving engraftment in HSPC transplantation, particularly in cases of limited cell doses such as CB transplantation and HSC gene therapy. Co-culturing monocytes increases survival and stemness of CB-CD34(+) cells. Soluble factors and direct-cell contact interactions play critical roles in the HSC-monocyte crosstalk.
BONE MARROW TRANSPLANTATION
(2022)
Article
Oncology
Yuan Wang, Ryohichi Sugimura
Summary: Hematopoietic stem cells (HSCs) are promising for treating various hereditary and hematologic diseases by differentiating into mature blood cells. However, the low population of HSCs in cord blood and bone marrow has led to investigations on ex vivo HSC expansion. This review surveys the current techniques for HSC self-renewal and summarizes the effects of intrinsic and extrinsic factors on HSC expansion, as well as the challenges and opportunities in this field.
EXPERIMENTAL CELL RESEARCH
(2023)
Article
Cell & Tissue Engineering
Valentina Orticelli, Andrea Papait, Elsa Vertua, Patrizia Bonassi Signoroni, Pietro Romele, Lorena Di Pietro, Marta Magatti, Luciana Teofili, Antonietta Rosa Silini, Ornella Parolini
Summary: This study investigated the potential of human term placenta-derived amniotic membrane MSCs (hAMSC) to support the ex vivo expansion of HSC and progenitor cells. The results suggest that hAMSCs are a valuable alternative to BM-MSC for enhancing the proliferation and maintaining stemness of CB-HSC, and can improve the colony forming unit ability and long-term culture initiating cells ability, addressing the need for high HSC content in CB units available for transplantation.
STEM CELLS TRANSLATIONAL MEDICINE
(2021)
Article
Cell & Tissue Engineering
Christoph Schaniel, Luena Papa, Marcia L. Meseck, Manisha Kintali, Mansour Djedaini, Mahtab Zangui, Camelia Iancu-Rubin, Ronald Hoffman
Summary: The study demonstrates the feasibility of cryopreserving VPA-expanded stem cell grafts derived from UCB-CD34+ cells for clinical utilization under cGMP. The cryopreserved expanded grafts showed high viability, retention of HSPC phenotypic subtypes, and long-term multilineage repopulation capacity in immunocompromised mice, with comparable cellular and functional parameters to fresh expanded products.
Article
Hematology
Bernhard Lehnertz, Jalila Chagraoui, Tara MacRae, Elisa Tomellini, Sophie Corneau, Nadine Mayotte, Isabel Boivin, Aurelie Durand, Deanne Gracias, Guy Sauvageau
Summary: This study identifies HLF as one of the most specific marker genes for human hematopoietic stem cells (HSCs) and develops a genomic reporter strategy to track its expression in blood cells. HLF-expressing cells are shown to comprise all stem cell activity during serial transplantation, establishing HLF as a defining gene of the human HSC state.
Article
Multidisciplinary Sciences
Serena Scala, Francesca Ferrua, Luca Basso-Ricci, Francesca Dionisio, Maryam Omrani, Pamela Quaranta, Raisa Jofra Hernandez, Luca Del Core, Fabrizio Benedicenti, Ilaria Monti, Stefania Giannelli, Federico Fraschetta, Silvia Darin, Elena Albertazzi, Stefania Galimberti, Eugenio Montini, Andrea Calabria, Maria Pia Cicalese, Alessandro Aiuti
Summary: This study found that hematopoietic stem/progenitor cells derived from mobilized peripheral blood and bone marrow have similar gene repair capabilities in ex vivo gene therapy for pediatric Wiskott-Aldrich syndrome. However, the peripheral blood group showed better treatment outcomes, with faster recovery and higher numbers of engrafted clones and gene corrections in the myeloid lineage, which are related to the higher amounts of primitive and myeloid progenitors in the peripheral blood-derived cells. This finding provides important references for the clinical interpretation of hematopoietic stem/progenitor cell transplantation outcomes.
NATURE COMMUNICATIONS
(2023)
Review
Hematology
Eran Zimran, Luena Papa, Ronald Hoffman
Summary: Ex vivo expansion of hematopoietic stem cells is a promising strategy to overcome the barrier of low stem cell dose, broaden the availability of cord blood units for transplantation, and optimize the clinical application of gene therapy.
Article
Cell & Tissue Engineering
Sara Bucar, Andre Dargen de Matos Branco, Marcia F. Mata, Joao Coutinho Milhano, Iris Caramalho, Joaquim M. S. Cabral, Ana Fernandes-Platzgummer, Claudia L. da Silva
Summary: The study compared the capacity of MSC from different sources to expand UCB CD34(+) cells ex vivo, with AT-derived MSC showing superior expansion potential and maintenance of primitive cells. UCM-derived MSC demonstrated inferior hematopoietic supportive capacity compared to MSC from adult tissues. Further research is needed to determine the impact of HPL on the hematopoietic supportive capacity of MSC.
STEM CELL RESEARCH & THERAPY
(2021)
Article
Cell & Tissue Engineering
Yuting Jin, Jie Huang, Qin Wang, Jiefeng He, Yincheng Teng, Rongzhen Jiang, Hal E. Broxmeyer, Bin Guo
Summary: The study demonstrates that RXR activity is crucial for ex vivo expansion of human cord blood HSCs, with HX531 promoting expansion and Bexarotene inhibiting it. This suggests that RXR serves as a negative regulator of ex vivo expansion of CB HSCs and HPCs.
STEM CELL REVIEWS AND REPORTS
(2021)
Article
Biochemistry & Molecular Biology
Ximena Bonilla, Ana Milena Lara, Manuela Llano-Leon, David A. Lopez-Gonzalez, David G. Hernandez-Mejia, Rosa Helena Bustos, Bernardo Camacho-Rodriguez, Ana-Maria Perdomo-Arciniegas
Summary: Umbilical cord blood is an important source of hematopoietic stem and progenitor cells, and recent research has focused on developing ex vivo expansion strategies for these cells. Co-culturing the cells with specific mesenchymal stromal cells has been found to significantly increase their cellular dose and regulate their proliferation capacity. Additionally, the secretory profile of umbilical cord blood-derived cells closely resembles that of bone marrow-derived cells.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Cell Biology
Chao-Ling Yao, Poyin Huang, Tsai-Chi Liu, Yung-Kai Lin, Ching-Yun Chen, Yi-Ting Lai, Tzu Yun Chin, Tsung-Yu Tseng, Yi-Chiung Hsu
Summary: Different experimental strategies for ex vivo expansion of human hematopoietic stem cells (HSCs) have been developed, however, the genomic function of expanded HSCs under different culture systems remains unclear. This study compared gene expression profiles of HSCs in serum and serum-free culture systems, identifying differentially expressed genes enriched in the TNF-regulated inflammatory pathway in the FBS culture system. The ex vivo expansion of HSCs using the FBS culture system induces an inflammatory response and high CD38 expression, providing new insights into genomic functions of HSCs under different expanded cultures.
Article
Engineering, Biomedical
Hye Sung Kim, Tzu-Chieh Ho, Moshe J. Willner, Michael W. Becker, Hae-Won Kim, Kam W. Leong
Summary: In this study, we propose an ex vivo T cell expansion system that mimics natural antigen-presenting cells for adoptive cell therapy. The system utilizes microfiber scaffolds coated with dendritic cell membrane to replicate the physicochemical properties of dendritic cells, leading to greater expansion and functionality of T cells. This scalable and customizable platform holds promise for future research and application in cell therapy.
BIOACTIVE MATERIALS
(2023)
Article
Multidisciplinary Sciences
Rashmi Bhardwaj, Lalit Kumar, Deepika Chhabra, N. K. Mehra, Atul Sharma, Sujata Mohanty, Vinod Kochupillai
Summary: This study explores the expansion of fetal liver hematopoietic stem cells in vitro, finding that a specific combination of 5 cytokines can optimally increase cell numbers with a cell viability greater than 90% throughout the 21-day culture period.
SCIENTIFIC REPORTS
(2021)
Article
Cell Biology
Hiroki Kawano, Yuko Kawano, Chen Yu, Mark W. LaMere, Matthew J. McArthur, Michael W. Becker, Scott W. Ballinger, Satoshi Gojo, Roman A. Eliseev, Laura M. Calvi
Summary: Mitochondrial dysfunction is common in various conditions, and mitochondrial DNA (mtDNA) transfer may be a promising strategy for treatment. In this study, we successfully expanded mouse hematopoietic stem cells (HSCs) ex vivo and observed high engraftment of the donor cells in the host. We also found that HSCs from donor mice could transfer mtDNA to host cells, highlighting the utility of ex vivo expanded HSCs for mitochondrial transfer.
Article
Critical Care Medicine
Luis A. Flores-Mejia, Graciela L. Cabrera-Rivera, Eduardo Ferat-Osorio, Ismael Mancilla-Herrera, Rafael Torres-Rosas, Ilka B. Bosco-Garate, Constantino Lopez-Macias, Armando Isibasi, Arturo Cerbulo-Vazquez, Lourdes A. Arriaga-Pizano
Article
Veterinary Sciences
Anaid Maciel Rivera, Susana Flores Villalva, Itzel Jimenez Vazquez, Omar Catalan Barcenas, Clara Espitia Pinzon, Julio Moran, Lourdes Arriaga-Pizano, Alejandro Benitez Guzman, Edgar Alfonseca Silva, Jose Angel Gutierrez-Pabello
VETERINARIA MEXICO
(2019)
Article
Obstetrics & Gynecology
Stephania Vazquez Rodriguez, Lourdes A. Arriaga Pizano, Estibalitz Laresgoiti Servitje, Javier Mancilla Ramirez, Omar L. Peralta Mendez, Gisela Villalobos Alcazar, Martha L. Granados Cepeda, Maria Graciela Hernandez Pelaez, Guadalupe Cordero Gonzalez, Roberto Arizmendi Villanueva, Jose Luis Cruz Ramirez, Armando Isibasi, Constantino Lopez Macias, Leopoldo Flores Romo, Luis A. Jimenez Zamudiog, Arturo Cerbulo-Vazquez
Summary: The study reveals that multiple markers, such as CD45RA, CD45RO, CD71, HLA-DR, and TREM-1, may be useful for diagnosing neonatal sepsis. However, only the expression of CD45RO on T lymphocytes is identified as a useful biomarker for diagnosing neonatal late-onset sepsis. Additionally, decision tree analyses suggest that CD45RO combined with CD27 could help differentiate preterm septic neonates from those with risk factors.
JOURNAL OF MATERNAL-FETAL & NEONATAL MEDICINE
(2021)
Article
Virology
Arturo Cerbulo-Vazquez, Lourdes Arriaga-Pizano, Gabriela Cruz-Cureno, Ilka Bosco-Garate, Eduardo Ferat-Osorio, Rodolfo Pastelin-Palacios, Ricardo Figueroa-Damian, Denisse Castro-Eguiluz, Javier Mancilla-Ramirez, Armando Isibasi, Constantino Lopez-Macias
Article
Integrative & Complementary Medicine
Lourdes Arriaga-Pizano, Daniel Cuauhtemoc Gomez-Jimenez, Luis Angel Flores-Mejia, Yobana Perez-Cervera, Carlos Josue Solorzano-Mata, Constantino Lopez-Macias, Armando Isibasi, Rafael Torres-Rosas
ACUPUNCTURE IN MEDICINE
(2020)
Article
Veterinary Sciences
Aldo Morales-Aguilar, Yahel Lopez-Reyes, Montserrat Regalado-Huitron, Rosa Elena Sarmiento-Silva, Lourdes Arriaga-Pizano, Alejandro Benitez-Guzman
RESEARCH IN VETERINARY SCIENCE
(2020)
Article
Multidisciplinary Sciences
Rosario Castro-Oropeza, Karla Vazquez-Santillan, Claudia Diaz-Gastelum, Jorge Melendez-Zajgla, Cecilia Zampedri, Eduardo Ferat-Osorio, Arturo Rodriguez-Gonzalez, Lourdes Arriaga-Pizano, Vilma Maldonado
SCIENTIFIC REPORTS
(2020)
Article
Immunology
Juan J. Montesinos, Lucero Lopez-Garcia, Victor A. Cortes-Morales, Lourdes Arriaga-Pizano, Ricardo Valle-Rios, Guadalupe R. Fajardo-Orduna, Marta E. Castro-Manrreza
JOURNAL OF IMMUNOLOGY RESEARCH
(2020)
Review
Biochemistry & Molecular Biology
Nadia Flores-Huerta, Macrina B. Silva-Cazares, Lourdes A. Arriaga-Pizano, Jessica L. Prieto-Chavez, Cesar Lopez-Camarillo
Summary: Breast cancer stem cells (bCSC), as a small subset of tumor cells with self-renewal properties and tumorigenic characteristics, play a key role in the development of breast cancer. Studies have shown that non-coding RNAs such as lncRNAs and miRNAs play important roles in regulating the stemness of bCSC.
Review
Medicine, Research & Experimental
Arturo Cerbulo Vazquez, Lourdes Arriaga-Pizano, Eduardo Ferat-Osorio
Summary: Sepsis, caused by pathogen invasion and unregulated response, poses a threat to patient lives with increasing incidence and high mortality rates globally. Understanding the role of cells and inflammatory mediators in host responses is crucial for developing medical treatments to regulate immune responses.
ARCHIVES OF MEDICAL RESEARCH
(2021)
Article
Computer Science, Interdisciplinary Applications
Lourdes Andrea Arriaga-Pizano, Marcos Angel Gonzalez-Olvera, Eduardo Antonio Ferat-Osorio, Jesica Escobar, Ana Luisa Hernandez-Perez, Cristina Revilla-Monsalve, Constatino Lopez-Macias, Jose Israel Leon-Pedroza, Graciela Libier Cabrera-Rivera, Uriel Guadarrama-Aranda, Ron Leder, Ana Gabriela Gallardo-Hernandez
Summary: The study proposed using a neural network to diagnose sepsis by utilizing common laboratory tests and vital signs with high sensitivity and specificity. The neural network system designed could accurately identify patients with sepsis, utilizing minimal resources and improving patient outcomes.
COMPUTER METHODS AND PROGRAMS IN BIOMEDICINE
(2021)
Article
Medicine, Research & Experimental
Ruth Lizzeth Madera-Sandoval, Arturo Cerbulo-Vazquez, Lourdes Andrea Arriaga-Pizano, Graciela Libier Cabrera-Rivera, Edna Basilio-Galvez, Patricia Esther Miranda-Cruz, Maria Teresa Garcia de la Rosa, Jessica Lashkmin Prieto-Chavez, Silvia Vanessa Rivero-Arredondo, Alonso Cruz-Cruz, Daniela Rodriguez-Hernandez, Maria Eugenia Salazar-Rios, Enrique Salazar-Rios, Esli David Serrano-Molina, Roberto Carlos De Lira-Barraza, Abel Humberto Villanueva-Compean, Alejandra Esquivel-Pineda, Ruben Ramirez-Montes de Oca, Omar Unzueta-Marta, Guillermo Flores-Padilla, Juan Carlos Anda-Garay, Luis Alejandro Sanchez-Hurtado, Salvador Calleja-Alarcon, Laura Romero-Gutierrez, Rafael Torres-Rosas, Laura C. Bonifaz, Rosana Pelayo, Edna Marquez-Marquez, Constantino I. I. I. Roberto Lopez-Macias, Eduardo Ferat-Osorio
Summary: The difficulty in predicting fatal outcomes in patients with COVID-19 impacts the burden on hospitals and their services. This study found that comorbidities such as hypertension and chronic renal failure were more common in deceased patients. The presence of mixed hypercytokinemia and a higher neutrophil-lymphocyte ratio were associated with increased risk of death.
CTS-CLINICAL AND TRANSLATIONAL SCIENCE
(2023)
Article
Cell Biology
Omar Escobar-Chavarria, Alejandro Benitez-Guzman, Itzel Jimenez-Vazquez, Jacobo Carrisoza-Urbina, Lourdes Arriaga-Pizano, Sara Huerta-Yepez, Guillermina Baay-Guzman, Jose A. Gutierrez-Pabello
Summary: The necrotic cell death induced by Mycobacterium bovis and the inflammasome activation in bovine macrophages help control bacterial growth.
Article
Cardiac & Cardiovascular Systems
Maria Jose Garces-Hernandez, Karen Pedraza-Escudero, Nayely Garibay-Nieto, Joselin Hernandez-Ruiz, Jessica Lakshmi Prieto-Chavez, Lourdes Andrea Arriaga-Pizano, Erendira Villanueva-Ortega, Galileo Escobedo, Aaron Noe Manjarrez-Reyna, Juan Carlos Lopez-Alvarenga, Jose Luis Perez-Hernandez, Gloria Queipo-Garcia
Summary: The expression of CCR2(+) monocyte subsets is significantly associated with subclinical atherosclerosis risk in boys, while in girls, no such association is observed.
JOURNAL OF CARDIOVASCULAR DEVELOPMENT AND DISEASE
(2022)
Article
Medicine, General & Internal
Lourdes Arriaga-Pizano, Dalia Ramirez-Ramirez, Jessica Prieto-Chavez, Rosana Pelayo, Alejandro Ruiz-Arguelles
GACETA MEDICA DE MEXICO
(2019)