Article
Cell Biology
Chun-Che Tseng, Shirley Dean, Brian A. Davies, Ishara F. Azmi, Natalya Pashkova, Johanna A. Payne, Jennifer Staffenhagen, Matt West, Robert C. Piper, Greg Odorizzi, David J. Katzmann
Summary: This study demonstrates the importance of the V domain of ESCRT-associated protein Bro1 in stimulating Vps4 for cargo sorting and ILV formation during MVB biogenesis, with ubiquitin binding enhancing this stimulation process. These findings reveal a novel mechanism for coordinating cargo sorting with membrane remodeling and ILV formation, providing insights into the roles of Bro1 homologues and ubiquitin in MVB sorting.
JOURNAL OF CELL BIOLOGY
(2021)
Review
Neurosciences
Christina Chatzi, Gary L. Westbrook
Summary: Dendritic spines, the distinctive postsynaptic feature of CNS excitatory synapses, play a crucial role in synaptic efficacy, plasticity, learning, and memory. This review focuses on I-BAR domain proteins expressed in the CNS, which are involved in sensing and introducing outward membrane curvature at synapses. These proteins have distinct functions in synapse formation and plasticity, and their interactions provide insights into neural plasticity and network rewiring.
FRONTIERS IN NEURAL CIRCUITS
(2021)
Article
Biochemistry & Molecular Biology
Rohan Dandage, Caroline M. Berger, Isabelle Gagnon-Arsenault, Kyung-Mee Moon, Richard Greg Stacey, Leonard J. Foster, Christian R. Landry
Summary: The study investigated protein-protein interactions in hybrids between yeast species, finding that most interactions are similar to those of the parents but with some altered instances. It suggests that the occurrence of chimeric protein complexes is frequent, potentially due to incompatibilities or imbalances between parental proteomes.
MOLECULAR BIOLOGY AND EVOLUTION
(2021)
Article
Biophysics
Zack Jarin, Alexander J. Pak, Patricia Bassereau, Gregory A. Voth
Summary: This study reveals the significant roles of protein curvature and lipid clustering in the relaxation of large membrane deformations. Lipid clusters mediate interactions between I-BAR domains and cause the formation of aggregates, leading to more cylindrical protrusions within membrane protrusions compared to irregularly shaped ones.
BIOPHYSICAL JOURNAL
(2021)
Article
Biotechnology & Applied Microbiology
M. Lairon-Peris, S. J. Routledge, J. A. Linney, J. Alonso-Del-Real, C. M. Spickett, A. R. Pitt, J. M. Guillamon, E. Barrio, A. D. Goddard, A. Querol
Summary: Researchers characterized 61 strains of Saccharomyces cerevisiae for ethanol tolerance and identified differences in ethanol sensitivity among different strains. The study revealed variations in membrane fluidity and permeability, suggesting a potential link between lipid composition and ethanol sensitivity.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Minttu S. Virolainen, Cecilie L. Soltoft, Per A. Pedersen, Lars Ellgaard
Summary: The research team successfully produced full-length FICD by combining a yeast platform and GFP tagging, providing a new approach for optimizing conditions for expression, solubilization, and purification. They also highlighted that this method is not only applicable to FICD, but also to other membrane proteins.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Ecology
Dikla Nachmias, Nataly Melnikov, Alvah Zorea, Maya Sharon, Reut Yemini, Yasmin De-picchoto, Ioannis Tsirkas, Amir Aharoni, Bela Frohn, Petra Schwille, Raz Zarivach, Itzhak Mizrahi, Natalie Elia
Summary: This study demonstrates that ESCRT proteins in the Asgard superphylum possess chromatin binding properties similar to those in eukaryotic cells, which may have important implications for the origin of eukaryogenesis.
Article
Chemistry, Multidisciplinary
Rui Jin, Michael Grasso, Mingyang Zhou, Ronen Marmorstein, Tobias Baumgart
Summary: The study characterizes the unfolding mechanism of the endophilin Al N-BAR domain during thermal denaturation and factors influencing its thermal stability. Results indicate a two-step thermal unfolding process with a dimeric intermediate displaying partial helicity loss. Deletion of the N-terminal H0 helix or the unstructured linker of endophilin decreased the melting temperature of the N-BAR domain, suggesting a role in structurally stabilizing the functional N-BAR domain in vivo. Single-site mutations were shown to compromise endophilin's thermal stability.
Article
Cell Biology
Zachary D. Stolp, Madhura Kulkarni, Yining Liu, Chengzhang Zhu, Alizay Jalisi, Si Lin, Arturo Casadevall, Kyle W. Cunningham, Fernando J. Pineda, Xinchen Teng, J. Marie Hardwick
Summary: This study reports a cell death pathway in yeast cells leading to vacuole/lysosome membrane permeabilization. The AP-3 complex plays a key role in promoting cell death, requiring its vesicle trafficking function and a specific kinase. The findings also suggest that this cell death mechanism is conserved in the human pathogenic yeast Cryptococcus neoformans.
Article
Microbiology
Philipp Schoeppner, Anne Pia Lutz, Bernard Johannes Lutterbach, Stefan Brueckner, Lars-Oliver Essen, Hans-Ulrich Moesch
Summary: This research elucidates the crystal structure of the extracellular cysteine-rich domain (CRD) of the yeast Wsc1 protein and identifies surface-exposed aromatic clusters in this domain. Mutational analysis demonstrates the essential role of these clusters in regulating the function of Wsc1 and the resistance of fungi to specific drugs and compounds.
Article
Multidisciplinary Sciences
Ira Buntenbroich, Vincent Anton, Daniel Perez-Hernandez, Tania Simoes, Felix Gaedke, Astrid Schauss, Gunnar Dittmar, Jan Riemer, Mafalda Escobar-Henriques
Summary: Defects in mitochondrial fusion play a role in many diseases. Mitofusins use self-interaction and GTP hydrolysis to drive membrane remodeling events. The mechanisms by which mitofusins mediate outer membrane fusion are still not fully understood, but structural studies provide insights and possible therapeutic interventions for mitofusin-associated diseases.
Review
Biotechnology & Applied Microbiology
Antonio Carlesso, Raquel Delgado, Oriol Ruiz Isant, Owens Uwangue, Dylan Valli, Roslyn M. Bill, Kristina Hedfalk
Summary: This study emphasizes the importance of yeast as a production host for eukaryotic membrane proteins. Yeast remains a useful host for studying α-helical membrane proteins, with both homologous and heterologous production being common in different yeast hosts.
FEMS YEAST RESEARCH
(2022)
Article
Multidisciplinary Sciences
Feng Yuan, Haleh Alimohamadi, Brandon Bakka, Andrea N. Trementozzi, Kasey J. Day, Nicolas L. Fawzi, Padmini Rangamani, Jeanne C. Stachowiak
Summary: "Membrane bending is primarily driven by proteins with specific structural features, such as amphipathic helices and crescent-shaped scaffolds. Recent studies show that disordered protein domains can form liquid phases on membrane surfaces, creating compressive stress that leads to membrane curvature and the formation of protein-lined membrane tubules."
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Biology
Sho W. Suzuki, Akihiko Oishi, Nadia Nikulin, Jeff R. Jorgensen, Matthew G. Baile, Scott D. Emr
Summary: The study uncovered a novel endosomal retrieval pathway mediated by Mvp1 protein, which deforms and cuts the endosomal membrane to release recycling tubules, distinct from traditional pathways. Additionally, human homolog SNX8, also implicated in Alzheimer's disease, participates in the formation of endosomal recycling tubules, revealing a conserved mechanism from yeast to humans.
Article
Multidisciplinary Sciences
David M. Hollenstein, Mariya Licheva, Nicole Konradi, David Schweida, Hector Mancilla, Muriel Mari, Fulvio Reggiori, Claudine Kraft
Summary: Vac8 plays a crucial role in coordinating the initiation of autophagosome formation by promoting the assembly of autophagy factors through avidity-driven interactions. In selective autophagy, Vac8 acts as a central hub to nucleate the phagophore assembly site at the vacuolar membrane, leading to clustering and sequestering of early autophagy factors and promoting phagophore assembly.
NATURE COMMUNICATIONS
(2021)
Article
Biochemistry & Molecular Biology
Iwona I. Smaczynska-de Rooij, Christopher J. Marklew, Ellen G. Allwood, Sarah E. Palmer, Wesley I. Booth, Ritu Mishra, Martin W. Goldberg, Kathryn R. Ayscough
MOLECULAR AND CELLULAR BIOLOGY
(2016)
Article
Immunology
Christina J. Gross, Ritu Mishra, Katharina S. Schneider, Guillaume Medard, Jennifer Wettmarshausen, Daniela C. Dittlein, Hexin Shi, Oliver Gorka, Paul-Albert Koenig, Stephan Fromm, Giovanni Magnani, Tamara Cikovic, Lara Hartjes, Joachim Smollich, Avril A. B. Robertson, Matthew A. Cooper, Marc Schmidt-Supprian, Michael Schuster, Kate Schroder, Petr Broz, Claudia Traidl-Hoffmann, Bruce Beutler, Bernhard Kuster, Juergen Ruland, Sabine Schneider, Fabiana Perocchi, Olaf Gross
Review
Cell Biology
Martin W. Goldberg
SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY
(2017)
Article
Multidisciplinary Sciences
Ines J. de Castro, James Budzak, Maria L. Di Giacinto, Lorena Ligammari, Ezgi Gokhan, Christos Spanos, Daniela Moralli, Christine Richardson, Jose I. de las Heras, Silvia Salatino, Eric C. Schirmer, Katharine S. Ullman, Wendy A. Bickmore, Catherine Green, Juri Rappsilber, Sarah Lamble, Martin W. Goldberg, Veronica Vinciotti, Paola Vagnarelli
NATURE COMMUNICATIONS
(2017)
Article
Cell Biology
Katharina S. Schneider, Christina J. Gross, Roland F. Dreier, Benedikt S. Saller, Ritu Mishra, Oliver Gorka, Rosalie Heilig, Etienne Meunier, Mathias S. Dick, Tamara Cikovic, Jan Sodenkamp, Guillaume Medard, Ronald Naumann, Juergen Ruland, Bernhard Kuster, Petr Broz, Olaf Gross
Article
Geriatrics & Gerontology
Mehmet U. Bikkul, Craig S. Clements, Lauren S. Godwin, Martin W. Goldberg, Ian R. Kill, Joanna M. Bridger
Article
Developmental Biology
Florian Geisler, Richard A. Coch, Christine Richardson, Martin Goldberg, Bernd Denecke, Olaf Bossinger, Rudolf E. Leube
Article
Ophthalmology
Antal Tapodi, Daniel M. Clemens, Alice Uwineza, Miguel Jarrin, Martin W. Goldberg, Emmanuelle Thinon, William P. Heal, Edward W. Tate, Karinne Nemeth-Cahalan, Irene Vorontsova, James E. Hall, Roy A. Quinlan
EXPERIMENTAL EYE RESEARCH
(2019)
Article
Cell Biology
Natalia Saiz-Ros, Rafal Czapiewski, Ilaria Epifano, Andrew Stevenson, Selene K. Swanson, Charles R. Dixon, Dario B. Zamora, Marion McElwee, Swetha Vijayakrishnan, Christine A. Richardson, Li Dong, David A. Kelly, Lior Pytowski, Martin W. Goldberg, Laurence Florens, Sheila V. Graham, Eric C. Schirmer
Article
Cell Biology
Marianne Grafe, Petros Batsios, Irene Meyer, Daria Lisin, Otto Baumann, Martin W. Goldberg, Ralph Graef
Article
Biochemistry & Molecular Biology
Christina James, Marret Mueller, Martin W. Goldberg, Christof Lenz, Henning Urlaub, Ralph H. Kehlenbach
JOURNAL OF BIOLOGICAL CHEMISTRY
(2019)
Article
Cell Biology
Georgia E. Hulmes, John D. Hutchinson, Noa Dahan, James M. Nuttall, Ellen G. Allwood, Kathryn R. Ayscough, Ewald H. Hettema
JOURNAL OF CELL BIOLOGY
(2020)
Article
Cell Biology
Eve Hunter-Featherstone, Natalie Young, Kathryn Chamberlain, Pablo Cubillas, Ben Hulette, Xingtao Wei, Jay P. Tiesman, Charles C. Bascom, Adam M. Benham, Martin W. Goldberg, Gabriele Saretzki, Iakowos Karakesisoglou
Summary: The study demonstrates that extracellular mechanics have significant effects on keratinocyte cell biology and should be utilized in skin research to ensure successful production of physiologically relevant skin models.
Article
Multidisciplinary Sciences
Charles R. Dixon, Poonam Malik, Jose de Las Heras, Natalia Saiz-Ros, Flavia de Lima Alves, Mark Tingey, Eleanor Gaunt, A. Christine Richardson, David A. Kelly, Martin W. Goldberg, Greg J. Towers, Weidong Yang, Juri Rappsilber, Paul Digard, Eric C. Schirmer
Summary: STING is an adaptor protein involved in cytoplasmic DNA sensing through cGAMP/cGAS, triggering innate immune responses. Upon transfection of dsDNA or dsRNA mimics, STING can redistribute from the inner nuclear membrane to the outer nuclear membrane, interacting with DNA- and RNA-binding proteins.