Article
Multidisciplinary Sciences
Cecilia Rodriguez-Furlan, Roya Campos, Jessica N. Toth, Jaimie M. Van Norman
Summary: Protein polarization is crucial for plant development processes. In this study, the authors demonstrate that IRK and KOIN, two LRR-receptor-kinases in the root meristem, have opposite polarities and rely on distinct mechanisms for polarization. Understanding the mechanisms of protein polarity and its link to cellular functions is important for studying plant development.
NATURE COMMUNICATIONS
(2022)
Review
Pharmacology & Pharmacy
Manyue Zhang, Na Xu, Wenxin Xu, Guixia Ling, Peng Zhang
Summary: With the development of nanotechnology, organelle-targeted nano drug delivery systems have emerged as a potential method to transport drugs specifically to subcellular compartments. Golgi-targeted nano drug delivery systems can alter the morphology of the Golgi apparatus and offer high specificity, low-dose administration, and decreased side effects. This review introduces Golgi-targeted nano drug delivery systems and their applications in disease therapies and diagnosis, including cancer, metastasis, fibrosis, and neurological diseases. It also discusses modifications and strategies to achieve targeting, Golgi-disturbing agents, and special endocytosis for endosomal/lysosomal escape.
PHARMACOLOGICAL RESEARCH
(2022)
Review
Biochemistry & Molecular Biology
Yihong Feng, Takuma Hiwatashi, Naoki Minamino, Kazuo Ebine, Takashi Ueda
Summary: Subcellular localization of proteins on endomembrane system is regulated by membrane trafficking. ANTH/ENTH/VHS protein family plays a role in various plant processes. This review summarizes the recent findings on these proteins in plants.
Article
Multidisciplinary Sciences
Simona Angerani, Eric Lindberg, Nikolai Klena, Christopher K. E. Bleck, Charlotte Aumeier, Nicolas Winssinger
Summary: The authors report the discovery of a fluorogenic small molecule substrate QPD-OTf for native kinesin-1 transport activity in live cells. In vitro assays show that Kinesin-1 and MTs can yield fluorescent crystals, while in cells, Kinesin-1 transport of cargo from the Golgi appears as trails of fluorescence over time.
NATURE COMMUNICATIONS
(2021)
Article
Plant Sciences
Madhumitha Narasimhan, Michelle Gallei, Shutang Tan, Alexander Johnson, Inge Verstraeten, Lanxin Li, Lesia Rodriguez, Huibin Han, Ellie Himschoot, Ren Wang, Steffen Vanneste, Judit Sanchez-Simarro, Fernando Aniento, Maciek Adamowski, Jiri Friml
Summary: This study reexamined the effects of auxin on PIN endocytic trafficking and found that high concentrations of NAA and IAA have nonspecific effects on the endomembrane system, while low concentrations specifically promoted endocytosis of PIN2. This contributes to polarity maintenance and highlights the nonspecific effects of high auxin levels on trafficking and endomembrane compartments.
Review
Cell Biology
Xiaoyan Zhang
Summary: The Golgi apparatus serves as a major processing station in the cell and requires a well-organized structure for proper protein glycosylation. Altered glycosylation is a hallmark of most cancer cells, and Golgi structural proteins are associated with tumor progression. Understanding Golgi structural and functional defects may help determine whether glycosylation defect is a cause or effect of oncogenesis.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Cell Biology
Artem A. Artykov, Anne Yagolovich, Dmitry A. Dolgikh, Mikhail P. Kirpichnikov, Daria B. Trushina, Marine E. Gasparian
Summary: TRAIL induces apoptosis through DR4 and DR5 on cell surface. Death receptors are rapidly internalized upon ligand stimulation and slowly return to plasma membrane after ligand is eliminated. Inhibition of receptor endocytosis sensitizes resistant cells to TRAIL and increases its cytotoxic activity against sensitive cells.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Multidisciplinary Sciences
Kendall Walton, Tomasz J. Nawara, Allyson R. Angermeier, Hadley Rosengrant, Eunjoo Lee, Bridge Wynn, Ekaterina Victorova, George Belov, Elizabeth Sztul
Summary: GBF1 is a critical cellular factor that is involved in various cellular processes and its participation in different cellular functions is regulated by specific phosphorylation of its conserved serine and tyrosine residues. The phosphorylation status of these residues differentially regulates GBF1's role in maintaining Golgi homeostasis and facilitating secretion versus its function in cytokinesis.
SCIENTIFIC REPORTS
(2023)
Article
Multidisciplinary Sciences
Mithu Baidya, Madhu Chaturvedi, Hemlata Dwivedi-Agnihotri, Ashutosh Ranjan, Dominic Devost, Yoon Namkung, Tomasz Maciej Stepniewski, Shubhi Pandey, Minakshi Baruah, Bhanupriya Panigrahi, Parishmita Sarma, Manish K. Yadav, Jagannath Maharana, Ramanuj Banerjee, Kouki Kawakami, Asuka Inoue, Jana Selent, Stephane A. Laporte, Terence E. Hebert, Arun K. Shukla
Summary: This study reveals that synthetic antibodies can be used to modulate GPCR trafficking and signaling in live cells.
NATURE COMMUNICATIONS
(2022)
Article
Multidisciplinary Sciences
Jachen Solinger, Harun-Or Rashid, Anne Spang
Summary: Cellular organization, compartmentalization, and cell-to-cell communication rely on endosomal pathways, and FERARI plays a crucial role in coordinating these pathways and regulating cargo flow through sorting endosomes via a kiss-and-run mechanism.
NATURE COMMUNICATIONS
(2022)
Article
Microbiology
Camilla Godlee, Ondrej E. Cerny, Mei Liu, Samkeliso W. Blundell, Alanna M. Gallagher, Meriam M. Shahin, David M. Holden
Summary: This study explores the targeting, membrane integration, and trafficking mechanisms of the Salmonella effector SteD. SteD integrates into the early secretory pathway membranes through two steps and hijacks a vesicular trafficking pathway to reach MHCII compartments. This research highlights the intricate interaction between bacterial pathogens and host cell biology at the molecular level.
Article
Plant Sciences
Deepanksha Arora, Daniel Van Damme
Summary: Endomembrane trafficking allows proteins and lipids to flow between different endomembrane compartments in eukaryotic cells, largely through vesicle-mediated transport. This process involves packaging transmembrane proteins into vesicles, followed by vesicle scission, uncoating, and fusion at the target membrane. Three major trafficking pathways (anterograde, retrograde, endocytic) operate inside cells, each involving specific machinery and coat proteins for packaging and delivery along the pathways.
Article
Plant Sciences
Josephine G. LoRicco, Li Kozel, Kaylee Bagdan, Ruby Epstein, David S. Domozych
Summary: In this study, the effects of endosidin 5 (ES5) on the Golgi apparatus and secretion of extracellular matrix (ECM) components in Penium margaritaceum were investigated. The results showed that ES5 had a significant impact on ECM secretion and affected the Golgi apparatus in a different way compared to other endomembrane inhibitors.
Article
Virology
Md. Saddam Hossain, Jason G. Kerkvliet, Adam D. Hoppe
Summary: Understanding cellular host factors that influence viral entry is crucial for developing viral countermeasures. This study presents a new strategy using CRISPR whole-genome screening on a pseudoviral platform to identify host genes specifically associated with viral entry steps. The approach increases screening specificity and successfully identifies receptors for SARS-CoV-2 and VSV-G.
JOURNAL OF MEDICAL VIROLOGY
(2023)
Article
Multidisciplinary Sciences
Junke Liu, Hengmin Tang, Chanjuan Xu, Shengnan Zhou, Xunying Zhu, Yuanyuan Li, Laurent Prezeau, Tao Xu, Jean-Philippe Pin, Philippe Rondard, Wei Ji, Jianfeng Liu
Summary: This study reveals that the assembly of platelet-activating factor receptor (PAFR) into dimers and oligomers can significantly influence its signaling mode. Oligomerization enhances G protein coupling, while restraining beta-arrestin recruitment and internalization.
NATURE COMMUNICATIONS
(2022)