Article
Multidisciplinary Sciences
Alexander E. Vlahos, Jeewoo Kang, Carlos A. Aldrete, Ronghui Zhu, Lucy S. Chong, Michael B. Elowitz, Xiaojing J. Gao
Summary: The authors have developed a modular approach called RELEASE to control intercellular signals using protein-based circuits. This design allows for diverse signal processing capabilities and has the potential to enable programmable delivery of intercellular cues in various fields such as neurobiology, cancer immunotherapy, and cell transplantation.
NATURE COMMUNICATIONS
(2022)
Article
Cell Biology
Philip John Robinson, Marie Anne Pringle, Bethany Fleming, Neil John Bulleid
Summary: Proteins in the secretory pathway require correct disulfide pairings for proper folding. Two important members of the protein disulfide isomerase (PDI) family, ERp57 and ERdj5, have unclear roles in disulfide formation, but ERp57 is involved in the isomerisation of non-native disulfides in glycoproteins. Alternative PDIs can compensate for the absence of ERp57 in isomerising glycoprotein disulfides, but only when a robust reductive pathway is present. ERdj5 is required for this alternative pathway as a reductase. These findings clarify the essential functions of two PDIs in the cell and highlight the distinctions between formation, reduction, and isomerisation of disulfide bonds.
JOURNAL OF CELL SCIENCE
(2023)
Article
Multidisciplinary Sciences
Yun Xiang, Rui Lyu, Junjie Hu
Summary: Membrane curvature is generated by constructing a curved oligomeric scaffold formed by endoplasmic reticulum tubule-forming proteins. The mechanism of curvature generation remains elusive.
NATURE COMMUNICATIONS
(2023)
Article
Microbiology
Anthony V. Morante, Daniel Davidnan Baboolal, Xavier Simon, Elizabeth, Mark Cheng-Ying Pan, Patricio I. Meneses
Summary: The amino acid residues 43-DQILQ-47 of the HPV16 L2 protein play a crucial role in the intracellular trafficking of the virus, particularly in trafficking beyond the endoplasmic reticulum.
MICROBIOLOGY SPECTRUM
(2022)
Review
Neurosciences
Taylor M. Benske, Ting-Wei Mu, Ya-Juan Wang
Summary: NMDARs are crucial for synaptic development and plasticity in the mammalian CNS. Proper folding and assembly of the receptors, as well as their trafficking to the plasma membrane, are ensured by protein quality control mechanisms.
FRONTIERS IN CELLULAR NEUROSCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Kohji Yamada, Kiyotsugu Yoshida
Summary: Unconventional protein secretion (UPS) is a crucial mechanism controlling the localization of cytosolic proteins lacking signal peptides and is implicated in inflammation, neurodegenerative diseases, and cancer. This article provides an overview of UPS mechanisms and categorizes cancer-specific UPS as CUPS due to their distinct features in cancer cells.
DNA AND CELL BIOLOGY
(2023)
Article
Chemistry, Multidisciplinary
Huiying Mu, Koji Miki, Takuya Kubo, Koji Otsuka, Kouichi Ohe
Summary: The new thiol probe VB1Cl, based on the meso-vinyl-BODIPY scaffold, showed significant fluorescence enhancement and high selectivity for biological thiol sensing. It was successfully utilized to monitor the protein unfolding process in living cells under ER stress.
CHEMICAL COMMUNICATIONS
(2021)
Review
Biology
Hidekatsu Yanai, Hiroki Adachi, Mariko Hakoshima, Hisayuki Katsuyama
Summary: Mitochondria are organelles that generate adenosine triphosphate through aerobic respiration. Imeglimin is a novel oral hypoglycemic agent that targets mitochondrial function and reduces reactive oxygen species production. It improves glucose metabolism, preserves pancreatic beta-cell mass, inhibits hepatic glucose production, and improves insulin sensitivity. Imeglimin also improves vascular dysfunction and reduces ischemia-induced brain damage. It can be a useful therapeutic option for diabetic complications in type 2 diabetic patients.
Article
Cell Biology
Keisuke Mochida, Toshifumi Otani, Yuto Katsumata, Hiromi Kirisako, Chika Kakuta, Tetsuya Kotani, Hitoshi Nakatogawa
Summary: In this study, the researchers uncovered the mechanism of action of the nucleophagy receptor Atg39 in the formation of NDVs. Atg39 is anchored to the outer nuclear membrane via its transmembrane domain and is also associated with the inner nuclear membrane through membrane-binding amphipathic helices, linking these membranes together. Additionally, the aggregation of Atg39 causes the nuclear envelope to protrude and pinch off to generate NDVs.
JOURNAL OF CELL BIOLOGY
(2022)
Article
Neurosciences
Christian Grinan-Ferre, Julia Jarne-Ferrer, Aina Bellver-Sanchis, Sandra Codony, Dolors Puigoriol-Illamola, Coral Sanfeliu, Yumin Oh, Seulki Lee, Santiago Vazquez, Merce Pallas
Summary: This study found that inhibiting soluble epoxide hydrolase (sEH) is helpful in combating neuroinflammation, and it can achieve neuroprotection by modulating the eIF2 alpha/CHOP pathway, which is of potential significance for the treatment of Alzheimer's disease.
CNS NEUROSCIENCE & THERAPEUTICS
(2023)
Article
Biochemistry & Molecular Biology
Weimin Yu, Siqi Li, Haixia Wu, Pingping Hu, Lili Chen, Chunyu Zeng, Xiaoyong Tong
Summary: The study showed that inhibiting sEH can alleviate atherosclerosis caused by endothelial Nox4 dysfunction, potentially through suppression of ER stress. Endothelial Nox4 dysfunction can lead to inflammation, and inhibiting sEH and ER stress is beneficial for alleviating atherosclerosis.
FREE RADICAL BIOLOGY AND MEDICINE
(2021)
Article
Multidisciplinary Sciences
Kohji Yamada, Saya Motohashi, Tsunekazu Oikawa, Naoko Tago, Rei Koizumi, Masaya Ono, Toshiaki Tachibana, Ayano Yoshida, Saishu Yoshida, Masayuki Shimoda, Masahiro Oka, Yoshihiro Yoneda, Kiyotsugu Yoshida
Summary: The study reveals the role of E-Syt1 in mediating cytosolic protein secretion at the ER-PM contact sites in liver cancer cells. SEC22B vesicles tethered to the PM via Q-SNAREs for their secretion, while PKC delta secretion and tumorigenicity were regulated by the interaction with E-Syt1.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Biochemistry & Molecular Biology
Karol Fiedorczuk, Jue Chen
Summary: Small molecule chaperones have been widely used as therapeutics for diseases caused by protein misfolding. CFTR correctors are the most successful examples, as they can revert folding defects and are used to treat cystic fibrosis. Cryo-electron microscopy structures reveal that these correctors stabilize TMD1 of CFTR, preventing premature degradation and rescuing disease-causing mutations.
Article
Biochemistry & Molecular Biology
Akira Hosomi, Chinatsu Okachi, Yudai Fujiwara
Summary: Soluble proteins with a signal peptide are translocated into the endoplasmic reticulum (ER), while some proteins without a signal peptide, like SOD1, can still enter the ER. SOD1 is a causative gene of ALS, and its secretion has been implicated in the pathogenesis of ALS. This study reveals that SOD1 is translocated into the ER lumen through the translocon Sec61 and subsequently secreted extracellularly, suggesting the potential of targeting SOD1 secretion as a therapeutic approach for ALS.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2023)
Review
Cell Biology
Meir Aridor
Summary: The realization that the meticulous organization of cellular organelles is not required for the reconstitution of select intracellular traffic steps has revolutionized cell biology. Recent advances in cellular and imaging technologies have shed new light on COPII activities and biosynthetic traffic between the ER and the Golgi.
Article
Multidisciplinary Sciences
Joshua Tan, Kathrin Pieper, Luca Piccoli, Abdirahman Abdi, Mathilde Foglierini, Roger Geiger, Claire Maria Tully, David Jarrossay, Francis Maina Ndungu, Juliana Wambua, Philip Bejon, Chiara Silacci Fregni, Blanca Fernandez-Rodriguez, Sonia Barbieri, Siro Bianchi, Kevin Marsh, Vandana Thathy, Davide Corti, Federica Sallusto, Peter Bull, Antonio Lanzavecchia
Article
Biochemistry & Molecular Biology
Roger Geiger, Jan C. Rieckmann, Tobias Wolf, Camilla Basso, Yuehan Feng, Tobias Fuhrer, Maria Kogadeeva, Paola Picotti, Felix Meissner, Matthias Mann, Nicola Zamboni, Federica Sallusto, Antonio Lanzavecchia
Article
Allergy
Desiree Schubert, Marie-Christine Klein, Sarah Hassdenteufel, Andres Caballero-Oteyza, Linlin Yang, Michele Proietti, Alla Bulashevska, Janine Kemming, Johannes Kuehn, Sandra Winzer, Stephan Rusch, Manfred Fliegauf, Alejandro A. Schaffer, Stefan Pfeffer, Roger Geiger, Adolfo Cavalie, Hongzhi Cao, Fang Yang, Yong Li, Marta Rizzi, Hermann Eibel, Robin Kobbe, Amy L. Marks, Brian P. Peppers, Robert W. Hostoffer, Jennifer M. Puck, Richard Zimmermann, Bodo Grimbacher
JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY
(2018)
Article
Biochemistry & Molecular Biology
Antonino Cassotta, Vincent Mikol, Thomas Bertrand, Stephanie Pouzieux, Josiane Le Parc, Paul Ferrari, Jacques Dumas, Michael Auer, Florian Deisenhammer, Matteo Gastaldi, Diego Franciotta, Chiara Silacci-Fregni, Blanca Fernandez Rodriguez, Isabella Giacchetto-Sasselli, Mathilde Foglierini, David Jarrossay, Roger Geiger, Federica Sallusto, Antonio Lanzavecchia, Luca Piccoli
Article
Biochemistry & Molecular Biology
Sean Michael Howard, Ilaria Ceppi, Roopesh Anand, Roger Geiger, Petr Cejka
NUCLEIC ACIDS RESEARCH
(2020)
Article
Immunology
Antonino Cassotta, Philipp Paparoditis, Roger Geiger, Ramgopal R. Mettu, Samuel J. Landry, Alessia Donati, Marco Benevento, Mathilde Foglierini, David J. M. Lewis, Antonio Lanzavecchia, Federica Sallusto
JOURNAL OF EXPERIMENTAL MEDICINE
(2020)
Article
Immunology
Tobias Wolf, Wenjie Jin, Giada Zoppi, Ian A. Vogel, Murodzhon Akhmedov, Christopher K. E. Bleck, Tim Beltraminelli, Jan C. Rieckmann, Neftali J. Ramirez, Marco Benevento, Samuele Notarbartolo, Dirk Bumann, Felix Meissner, Bodo Grimbacher, Matthias Mann, Antonio Lanzavecchia, Federica Sallusto, Ivo Kwee, Roger Geiger
Article
Multidisciplinary Sciences
Tiziano Bernasocchi, Geniver El Tekle, Marco Bolis, Azzurra Mutti, Arianna Vallerga, Laura P. Brandt, Filippo Spriano, Tanya Svinkina, Marita Zoma, Valentina Ceserani, Anna Rinaldi, Hana Janouskova, Daniela Bossi, Manuela Cavalli, Simone Mosole, Roger Geiger, Ze Dong, Cai-Guang Yang, Domenico Albino, Andrea Rinaldi, Peter Schraml, Simon Linder, Giuseppina M. Carbone, Andrea Alimonti, Francesco Bertoni, Holger Moch, Steven A. Carr, Wilbert Zwart, Marianna Kruithof-de Julio, Mark A. Rubin, Namrata D. Udeshi, Jean-Philippe P. Theurillat
Summary: Mutually exclusive prostate cancer driver alterations involving ERG transcription factor and ubiquitin ligase adaptor SPOP have synthetic sick effects, regulating the response to therapeutic interventions in the AR pathway. This dichotomy highlights a distinct class of antagonistic cancer drivers and provides a blueprint for therapeutic exploitation.
NATURE COMMUNICATIONS
(2021)
Article
Multidisciplinary Sciences
Yiwei Chen, Kai Xu, Luca Piccoli, Mathilde Foglierini, Joshua Tan, Wenjie Jin, Jason Gorman, Yaroslav Tsybovsky, Baoshan Zhang, Boubacar Traore, Chiara Silacci-Fregni, Claudia Daubenberger, Peter D. Crompton, Roger Geiger, Federica Sallusto, Peter D. Kwong, Antonio Lanzavecchia
Summary: This study demonstrates that Plasmodium falciparum RIFINs can bind to LILRB1 through D3, and inserting receptor domains into the VH-CH1 elbow can generate novel antibodies, as shown by a naturally selected example.
Article
Oncology
Valentina Mele, Camilla Basso, Valeria Governa, Jesus F. Glaus Garzon, Manuele G. Muraro, Silvio Daester, Christian A. Nebiker, Robert Mechera, Martin Bolli, Alexander Schmidt, Roger Geiger, Giulio C. Spagnoli, Dimitri Christoforidis, Pietro E. Majno, Lubor Borsig, Giandomenica Iezzi
Summary: Non-transformed cells of the tumor microenvironment, such as cancer-associated fibroblasts (CAFs) or tumor-associated stromal cells (TASCs), play a role in the growth and prognosis of colon cancer. By comparing the proteome of TASCs derived from colon cancer tissue with that of healthy colon tissue, two novel markers highly associated with poor prognosis were identified.
Article
Biology
Hanif J. Khameneh, Nicolas Fonta, Alessandro Zenobi, Charlene Niogret, Pedro Ventura, Concetta Guerra, Ivo Kwee, Andrea Rinaldi, Matteo Pecoraro, Roger Geiger, Andrea Cavalli, Francesco Bertoni, Eric Vivier, Andreas Trumpp, Greta Guarda
Summary: This study reveals the importance of MYC in NK cells and its role in regulating translation and expansion, as well as anticancer immunity. Deficiency of MYC in NK cells results in reduced numbers and impaired cytotoxic functions of mature NK cells.
LIFE SCIENCE ALLIANCE
(2023)
Article
Genetics & Heredity
Murodzhon Akhmedov, Axel Martinelli, Roger Geiger, Ivo Kwee
NAR GENOMICS AND BIOINFORMATICS
(2020)
Article
Biology
Alessia Landi, Muriel Mari, Svenja Kleiser, Tobias Wolf, Christine Gretzmeier, Isabel Wilhelm, Dimitra Kiritsi, Roland Thuenauer, Roger Geiger, Alexander Nystroem, Fulvio Reggiori, Julie Claudinon, Winfried Roemer
LIFE SCIENCE ALLIANCE
(2019)
Article
Medicine, Research & Experimental
Adria-Arnau Marti Lindez, Isabelle Dunand-Sauthier, Mark Conti, Florian Gobet, Nicolas Nunez, J. Thomas Hannich, Howard Riezman, Roger Geiger, Alessandra Piersigilli, Kerstin Hahn, Sylvain Lemeille, Burkhard Becher, Thibaut De Smedt, Stephanie Hugues, Walter Reith
Article
Immunology
Jan C. Rieckmann, Roger Geiger, Daniel Hornburg, Tobias Wolf, Ksenya Kveler, David Jarrossay, Federica Sallusto, Shai S. Shen-Orr, Antonio Lanzavecchia, Matthias Mann, Felix Meissner