Article
Biochemistry & Molecular Biology
Yu-Syuan Luo, Tai-Ying Long, Su-Yin Chiang, Kuen-Yuh Wu
Summary: This study investigated the toxicokinetics of AA, GA2, and GA3-GSH in Sprague Dawley rats, showing rapid formation, elimination, and linear kinetics of these conjugates. The values of AA-and GA-GSHs represent direct detoxification of AA and GA in vivo, providing insights into sex and inter-species differences in AA detoxification.
CHEMICO-BIOLOGICAL INTERACTIONS
(2021)
Article
Pharmacology & Pharmacy
Melanie Hemgesberg, Simone Stegmuller, Alexander Cartus, Dieter Schrenk
Summary: The study found that a background level of N7-GA-Gua adducts exists in untreated hepatocytes, and incubation with AA at concentrations below 500μM did not result in a significant increase in adduct levels. However, significant increases in N7-GA-Gua levels were found at concentrations above 500μM, and BMC modeling of the data showed non-linear concentration-response curves, indicating that AA only started to cause measurable increases in N7-GA-Gua levels above certain concentrations.
Review
Food Science & Technology
Thierry Delatour, Richard H. Stadler
Summary: Significant progress has been made in understanding the formation, reduction, and cancer risk of acrylamide in foods over the past two decades. This review highlights new discoveries in formation and analysis, regulations in the European Union, and considerations for reevaluating the toxicity of acrylamide.
CRITICAL REVIEWS IN FOOD SCIENCE AND NUTRITION
(2022)
Article
Environmental Sciences
Guangli Yin, Shengen Liao, Dexing Gong, Hongxia Qiu
Summary: The study found that HbAA was linearly and inversely associated with the risk of DM, while HbGA/HbAA was nonlinearly and positively associated with the prevalence of DM. These results support epidemiological evidence that HbAA and HbGA/HbAA are significantly associated with diabetes mellitus.
ENVIRONMENTAL POLLUTION
(2021)
Article
Environmental Sciences
Iokfai Cheang, Shengen Liao, Xu Zhu, Xinyi Lu, Qingqing Zhu, Wenming Yao, Yanli Zhou, Haifeng Zhang, Xinli Li
Summary: The study found an association between acrylamide (AA) hemoglobin biomarkers and serum lipid levels, particularly with triglycerides (TG) showing a linear correlation. Additionally, there was a positive correlation between HbGA/HbAA and low-density lipoprotein-cholesterol (LDL-C), and a negative correlation with high-density lipoproteincholesterol (HDL-C).
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2021)
Article
Food Science & Technology
Anli Wang, Xinyu Chen, Laizhao Wang, Wei Jia, Xuzhi Wan, Jingjing Jiao, Weixuan Yao, Yu Zhang
Summary: The study found that (-)-epigallocatechin gallate (EGCG) and (-)-epicatechin (EC) can protect liver cells from the damage caused by acrylamide (AA) and glycidamide (GA). They can inhibit cellular apoptosis and DNA damage and promote cell cycle progression.
FOOD AND CHEMICAL TOXICOLOGY
(2022)
Article
Environmental Sciences
Jingjia Liang, Cheng Xu, Qian Liu, Zhenkun Weng, Xin Zhang, Jin Xu, Aihua Gu
Summary: This study found a positive association between acrylamide exposure, particularly its metabolite, glycidamide, and the prevalence of hypertension or increased systolic blood pressure in adolescent females. Total cholesterol was found to mediate a portion of this association.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2022)
Article
Agriculture, Multidisciplinary
Zhaoying Li, Jing Sun, Dongfeng Zhang
Summary: The study found a positive association between hemoglobin adduct levels of acrylamide and depressive symptoms, but an inverse association between acrylamide and its metabolite. Especially, the association between acrylamide and depressive symptoms was strengthened in smokers, in age 18-39 and 40-59 years and BMI 25-30 kg/m(2) groups. Further investigations are needed to study the associations between hemoglobin adducts of acrylamide, glycidamide, and depressive symptoms.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2021)
Article
Food Science & Technology
Melanie Hemgesberg, Simone Stegmueller, Alexander Cartus, Selina Hemmer, Michael Puettmann, Jean-Pierre Stockis, Dieter Schrenk
Summary: The study found that the presence of AA-derived DNA adducts in human PBMC is related to body weight rather than specific dietary or lifestyle factors.
FOOD AND CHEMICAL TOXICOLOGY
(2021)
Article
Food Science & Technology
T. R. Reshmitha, P. Nisha
Summary: The study found that lycopene (LYC) can enhance the antioxidant defense against acrylamide (ACR) and glycidamide (GLY) induced toxicity in liver cells, by increasing cell viability, reducing reactive oxygen species production, and enhancing antioxidant enzyme activity.
JOURNAL OF FUNCTIONAL FOODS
(2021)
Article
Environmental Sciences
Yaoran Li, Jiahao Jiang, Qiao Wang, Li Zhu, Wei Jia, Xinyu Chen, Yu Zhang
Summary: Acrylamide, a probable carcinogen, has been widely used and detected in water treatment and thermal processing foods. A physiologically based toxicokinetics (PBTK) model has been developed to accurately estimate the internal dosimetry and metabolic process of acrylamide intake in humans.
Article
Environmental Sciences
Michael Poteser, Federica Laguzzi, Thomas Schettgen, Nina Vogel, Till Weber, Aline Murawski, Phillipp Schmidt, Maria Ruther, Marike Kolossa-Gehring, Sonia Namorado, An Van Nieuwenhuyse, Brice Appenzeller, Edda Dufthaksdottir, Kristin Olafsdottir, Line Smastuen Haug, Cathrine Thomsen, Fabio Barbone, Valentina Rosolen, Loic Rambaud, Margaux Riou, Thomas Goen, Stefanie Nuebler, Moritz Schaefer, Karin H. A. Zarrabi, Liese Gilles, Laura Rodriguez Martin, Greet Schoeters, Ovnair Sepai, Eva Govarts, Hanns Moshammer
Summary: This report summarizes the results of the HBM4EU project and associated studies regarding acrylamide exposure levels. The findings show an increasing trend in acrylamide exposure in children, while the trend is less significant in adults and smokers. The average acrylamide exposure still exceeds suggested benchmark levels, raising specific concerns for children. Further research is needed to confirm the declining trend observed in recent years.
Article
Environmental Sciences
Michael Poteser, Federica Laguzzi, Thomas Schettgen, Nina Vogel, Till Weber, Philipp Zimmermann, Domenica Hahn, Marike Kolossa-Gehring, Sonia Namorado, An Van Nieuwenhuyse, Brice Appenzeller, Thorhallur I. Halldorsson, Asa Eiriksdottir, Line Smastuen Haug, Cathrine Thomsen, Fabio Barbone, Valentina Rosolen, Loic Rambaud, Margaux Riou, Thomas Goeen, Stefanie Nuebler, Moritz Schaefer, Karin Haji Abbas Zarrabi, Liese Gilles, Laura Rodriguez Martin, Greet Schoeters, Ovnair Sepai, Eva Govarts, Hanns Moshammer
Summary: Acrylamide, a potential carcinogen, has been found in common dietary products and tobacco smoke. The European Commission has taken measures to reduce its content in food. The HBM4EU project shows an overall increase in acrylamide exposure between 2001 and 2017, but a decline in exposure values after 2018. Regional differences do not affect the overall time trend of exposure.
Review
Environmental Sciences
Janneke Hogervorst, Ana Virgolino, Thorhallur Halldorsson, Marco Vinceti, Agneta Akesson, Karin Leander, Tim Nawrot, Tommaso Filippini, Federica Laguzzi
Summary: This study conducted a systematic review and dose-response meta-analysis to evaluate the relationship between maternal acrylamide exposure during pregnancy and birth outcomes. The results showed that higher levels of acrylamide exposure were associated with increased risk of adverse birth outcomes.
ENVIRONMENTAL RESEARCH
(2022)
Article
Public, Environmental & Occupational Health
Euridice Martinez Steele, Jessie P. Buckley, Carlos Augusto Monteiro
Summary: This study aimed to examine the association between dietary energy contribution of ultra-processed food (UPF) and acrylamide exposure in the US. The study found that UPF consumption was positively associated with acrylamide exposure, which is known to be linked to cardiovascular disease and cancer.
PREVENTIVE MEDICINE
(2023)
Article
Toxicology
Si Chen, Qiangen Wu, Xilin Li, Dongying Li, Michelle Fan, Zhen Ren, Matthew Bryant, Nan Mei, Baitang Ning, Lei Guo
ARCHIVES OF TOXICOLOGY
(2020)
Article
Toxicology
Xilin Li, Xiaobo He, Si Chen, Yuan Le, Matthew S. Bryant, Lei Guo, Kristine L. Witt, Nan Mei
Summary: This study systematically evaluated the cytotoxicity and genotoxicity of luteolin in human lymphoblastoid cells, and found that the presence of specific cytochrome P450 enzymes, CYP1A1 and 1A2, enhanced the toxicity of luteolin by partially reversing its metabolic conversion process.
TOXICOLOGY LETTERS
(2021)
Article
Toxicology
Ji-Eun Seo, Xiaoqing Guo, Dayton M. Petibone, Sharon D. Shelton, Ying Chen, Xilin Li, Volodymyr Tryndyak, Stephanie L. Smith-Roe, Kristine L. Witt, Nan Mei, Mugimane G. Manjanatha
Summary: The study confirmed the induction of micronuclei by black cohosh extract in TK6 and HepG2 cells, as well as DNA damage in TK6 cells. Exposure to 125 µg/ml BCE (24 h) led to G1/S arrest in TK6 cells, accompanied by apoptosis and increased expression of related proteins.
TOXICOLOGICAL SCIENCES
(2021)
Article
Genetics & Heredity
Fanxue Meng, Nan Mei, Jian Yan, Xiaoqing Guo, Patricia A. Richter, Tao Chen, Mamata De
Summary: In this study, quantitative analysis using BMD modeling was used to evaluate the mutagenic potency of six whole smoke solutions (WSS) in the Ames test. The results showed that differences in smoking-machine regimen (ISO vs. CI) and cigarette product can influence the mutagenic potency of WSS.
Article
Biotechnology & Applied Microbiology
Timothy W. Robison, Robert H. Heflich, Mugimane G. Manjanatha, Rosalie Elespuru, Aisar Atrakchi, Nan Mei, Wei Ding
Summary: The recommendations made by Kirkland et al. propose using in vivo comet and transgenic rodent gene mutation assays to screen for in vivo mutagenicity. The in vivo follow-up for an in vitro bacterial mutation-positive drug should be based upon evaluating a similar endpoint (i.e., mutagenicity) to determine if the findings of in vitro gene mutation correlate with in vivo gene mutation. The most scientifically appropriate in vivo assays would be the TGR mutation assay or, in some circumstances, the in vivo Pig-a assay.
MUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS
(2021)
Article
Biotechnology & Applied Microbiology
R. A. Mittelstaedt, J. G. Shaddock, J. A. Bhalli, X. Guo, Y. Li, N. Mei, M. De, P. A. Richter, R. H. Heflich
Summary: The dose-response modeling of in vitro micronucleus test (IVMNT) data was evaluated to determine its value in discriminating among different tobacco products. The results indicated that BMD potency ranking was useful for differentiating between IVMNT responses.
MUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS
(2021)
Article
Pharmacology & Pharmacy
Ji-Eun Seo, Kelly Davis, Pritpal Malhi, Xiaobo He, Matthew Bryant, John Talpos, Susan Burks, Nan Mei, Xiaoqing Guo
Summary: Non-human primates (NHPs) have been crucial in various studies due to their physiological and genetic similarity to humans. This study isolated primary hepatocytes from rhesus macaques and compared their sensitivity and specificity in detecting genotoxic/carcinogenic compounds with primary human hepatocytes. Despite differences in CYP450 enzyme activities, the results showed that PMHs can be a reliable surrogate for PHHs in evaluating genotoxic hazards.
Article
Toxicology
Yuan Le, Xilin Li, Si Chen, Kylie G. Ning, Xiaoqing Guo, Charles G. Wu, Mugimane G. Manjanatha, Nan Mei
Summary: Black cohosh extract (BCE) has been widely used to relieve menopausal symptoms, but recent studies have shown that it is not effective and may even cause genotoxicity. This study focused on one of the constituents of BCE called actein, which was found to have high cytotoxicity and genotoxicity. Actein caused DNA damage, activated DNA damage response pathway, and induced cell cycle changes and apoptosis. Both BCE and actein also increased reactive oxygen species production and activated the MAPK signaling pathway. The genotoxicity induced by BCE and actein was partially mediated through oxidative stress.
JOURNAL OF APPLIED TOXICOLOGY
(2022)
Article
Toxicology
Ji-Eun Seo, Xilin Li, Yuan Le, Nan Mei, Tong Zhou, Xiaoqing Guo
Summary: This study compared the performance of HepaRG cells grown in 2D and 3D cultures in the high-throughput flow cytometry-based micronucleus (MN) assay. The results showed that 3D HepaRG spheroids exhibited higher sensitivity in detecting genotoxicants requiring metabolic activation. Integration of the MN and comet assays improved the sensitivity for detecting such genotoxicants, suggesting that HepaRG spheroids may contribute to New Approach Methodologies for genotoxicity assessment.
ARCHIVES OF TOXICOLOGY
(2023)
Article
Toxicology
Si Chen, Xilin Li, Yuxi Li, Xiaobo He, Matthew Bryant, Xuan Qin, Feng Li, Ji-Eun Seo, Xiaoqing Guo, Nan Mei, Lei Guo
Summary: Lapatinib, a drug used for treating HER2-positive breast cancer, has been associated with hepatotoxicity. This study shows that lapatinib causes cytotoxicity in multiple types of hepatic cells and induces cell cycle disturbances, apoptosis, and DNA damage. The metabolism of lapatinib by cytochrome P450 (CYP) enzymes, particularly CYP3A5 and 3A7, plays a role in attenuating its cytotoxicity. Furthermore, one of the metabolites, N-dealkylated lapatinib, is more toxic than the parent drug and other metabolites.
TOXICOLOGICAL SCIENCES
(2023)
Article
Oncology
Xilin Li, Yuxi Li, Kylie G. Ning, Si Chen, Lei Guo, Jessica A. Bonzo, Nan Mei
Summary: In vitro genotoxicity testing is crucial for chemical risk assessment, and TK6 cells serve as a standard cell line for regulatory safety evaluations. This study surveyed the baseline expression of major Phase II drug-metabolizing enzymes in TK6 cells, providing a reference enzymatic profile for this commonly used cell line.
JOURNAL OF ENVIRONMENTAL SCIENCE AND HEALTH PART C-TOXICOLOGY AND CARCINOGENESIS
(2022)
Meeting Abstract
Environmental Sciences
X. Guo, Je Seo, Dm Petibone, Sd Shelton, Y. Chen, X. Li, V Tryndyak, Sl Smith-Roe, Kl Witt, N. Mei, Mg Manjanatha
ENVIRONMENTAL AND MOLECULAR MUTAGENESIS
(2021)
Meeting Abstract
Environmental Sciences
X. Li, X. He, S. Chen, X. Guo, M. S. Bryant, L. Guo, M. G. Manjanatha, T. Zhou, K. L. Witt, N. Mei
ENVIRONMENTAL AND MOLECULAR MUTAGENESIS
(2021)
Meeting Abstract
Environmental Sciences
K. L. Witt, G. Chen, T. Cheairs, G. Eisenbrand, J. T. MacGregor, N. Mei, I. M. C. M. Rietjens, S. L. Smith-Roe, H. Stopper, Y. Thakkar, J. van Benthem, D. Xi, E. Zeiger, S. Pfuhler
ENVIRONMENTAL AND MOLECULAR MUTAGENESIS
(2021)
Article
Oncology
Si Chen, Qiangen Wu, Xilin Li, Dongying Li, Nan Mei, Baitang Ning, Montserrat Puig, Zhen Ren, William H. Tolleson, Lei Guo
Summary: This study confirmed the stability of CYP-overexpressing HepG2 cell lines in long-term cultures and demonstrated consistency in response to stimuli, providing valuable information for the broader use of these cells in pharmacologic and toxicologic research.
JOURNAL OF ENVIRONMENTAL SCIENCE AND HEALTH PART C-ENVIRONMENTAL CARCINOGENESIS & ECOTOXICOLOGY REVIEWS
(2021)