Article
Biochemistry & Molecular Biology
Elle Koivunotko, Jasmi Snirvi, Arto Merivaara, Riina Harjumaki, Swarna Rautiainen, Minna Kelloniemi, Kirsi Kuismanen, Susanna Miettinen, Marjo Yliperttula, Raili Koivuniemi
Summary: Adipose-derived mesenchymal stromal cells (ASCs) hold great potential for cellular therapies and can differentiate into endothelial cells (ECs) and other angiogenic cell types. Nanofibrillated cellulose (NFC) hydrogel is a suitable matrix for 3D cell culture of ASCs and can stimulate angiogenesis. This study recommends the use of NFC hydrogel as an animal-free biomaterial scaffold for therapeutic angiogenesis and other cell therapy purposes.
Article
Surgery
Huaian Lin, Yifan Zhang, Li Yu, Danning Zheng, Juan An
Summary: In this study, Salvia miltiorrhiza injection was found to enhance adipogenic differentiation of adipose-derived stem cells, potentially improving fat graft survival. However, further in vivo research is needed to fully understand the effects of this treatment on fat graft outcomes.
PLASTIC AND RECONSTRUCTIVE SURGERY
(2021)
Article
Anatomy & Morphology
Zhijie Liang, Donglin Huang, Wenhai Nong, Jinping Mo, Dandan Zhu, Mengxin Wang, Maojian Chen, Changyuan Wei, Hongmian Li
Summary: This study aimed to identify the optimal concentrations of A-PRFe to promote adipogenic and osteogenic differentiation of human adipose-derived stem cells (ASCs). Results showed that A-PRFe significantly stimulated ASC proliferation and enhanced adipogenic/osteogenic differentiation and paracrine function at higher concentrations.
Article
Engineering, Biomedical
Mansoor Chaaban, Adrien Moya, Andres Garcia-Garcia, Robert Paillaud, Romain Schaller, Thibaut Klein, Laura Power, Katarzyna Buczak, Alexander Schmidt, Elisabeth Kappos, Tarek Ismail, Dirk J. Schaefer, Ivan Martin, Arnaud Scherberich
Summary: This study investigates the effects of cell expansion and chondrogenic maturation degree on the process of endochondral ossification (ECO). The results show that the maturation degree of adipose-derived stromal cells (ASC) is closely related to their ability to form bone in vivo, with ECO achieved only for HCTs of intermediate maturation. Meanwhile, the expansion of ASC also results in the enrichment of the stromal fraction, and inducing quiescence can rescue their chondrogenic potential.
Article
Biochemistry & Molecular Biology
Shuang Zhang, Jeroen van de Peppel, Marijke Koedam, Johannes P. T. M. van Leeuwen, Bram C. J. van der Eerden
Summary: In this study, TNS3 was found to be involved in the fate decision of human bone marrow-derived stromal cells (BMSCs), regulating osteogenesis and adipogenesis through the control of RhoA activity and cytoskeletal reorganization. The findings highlight the crucial role of TNS3 in bone metabolism.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2023)
Article
Materials Science, Multidisciplinary
Jong Bo Park, Ji Yeon Ahn, Woo Sub Yang, Seong Chae Park, Young Jin Jung, Myungook Lee, Byung Hee Hong, Jeong Mook Lim
Summary: This study investigated the effect of nanoscale spacing by stacked graphene film on osteogenic differentiation of human adipose-derived mesenchymal stromal cells. The results showed that osteogenic differentiation was promoted as the stacking layer increased, with the most significant effects observed in the three layers. The study demonstrated that multilayer-stacked graphene created nanoscale spaces promote calcium deposit and cytoskeletal integrity in hADSC-related, in vitro-osteogenesis.
Article
Cell Biology
Isis Cortes, Gutemberg Alves, Cesar Claudio-Da-Silva, Leandra Santos Baptista
Summary: This study found that different subpopulations of adipose stem/stromal cells (ASCs) exhibit distinct physiological properties in a 3D spheroid culture. ASC spheroids from superficial subcutaneous adipose tissue (sSAT) showed the highest response to adipogenic induction, while spheroids from deep subcutaneous adipose tissue (dSAT) showed the greatest lipolytic capacity. Spheroids from superficial retinacula cutis (sRC) exhibited a greater secretory capacity.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Enrico C. Torre, Mesude Bicer, Graeme S. Cottrell, Darius Widera, Francesco Tamagnini
Summary: Adipose-derived mesenchymal stromal cells (ASCs) have the potential to differentiate into various cell types and exhibit spontaneous Ca2+ oscillations. The effects of differentiation treatments on ASCs' Ca2+ dynamics, in terms of oscillation fraction and frequency, were examined in this study. Both adipogenic and osteogenic differentiation resulted in reduced Ca2+ dynamics, with adipogenic differentiation showing a more pronounced effect. Changes in Ca2+ oscillations were observed as early as 7 days in vitro (DIV). Further research is needed to understand the molecular and mechanistic correlates of these changes for the development of interventions in regenerative medicine.
Article
Cell Biology
Furkan Yigitbilek, Sabena M. Conley, Hui Tang, Ishran M. Saadiq, Kyra L. Jordan, Lilach O. Lerman, Timucin Taner
Summary: MSCs from liver and adipose tissue showed similar proliferation and migration properties, but had differences in immunomodulatory and pro-angiogenic abilities. Selecting cell-based therapy according to the specific characteristics of different cell sources may be important for treating certain diseases.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Biology
Gillian Fitzgerald, Guillermo Turiel, Tatiane Gorski, Ines Soro-Arnaiz, Jing Zhang, Nicola C. Casartelli, Evi Masschelein, Nicola A. Maffiuletti, Reto Sutter, Michael Leunig, Jean Farup, Katrien De Bock
Summary: In this study, the heterogeneity of FAP cells in patients with fatty infiltration was characterized using single-nuclei and single-cell RNA sequencing. An MME+ FAP subpopulation with high adipogenic potential was identified, which showed resistance to WNT activators. It was also found that many MME+ FAP cells undergo apoptosis during muscle regeneration and differentiate into adipocytes under pathological conditions.
COMMUNICATIONS BIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Jianhua Liu, Yutong Che, Ke Cai, Bishi Zhao, Liying Qiao, Yangyang Pan, Kaijie Yang, Wenzhong Liu
Summary: This study investigates the role of miR-136 and HSD17B12 in adipogenesis of ovine SVFs and their relationship. The results suggest that miR-136 promotes proliferation and inhibits adipogenic differentiation, while HSD17B12 has the opposite effects. These findings provide insights into the regulatory mechanisms of lipid deposition in sheep.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Cell & Tissue Engineering
Christian Ploner, Tina Rauchenwald, Catherine E. Connolly, Karin Joehrer, Johannes Rainer, Christof Seifarth, Martin Hermann, Markus Nagl, Susanne Lobenwein, Doris Wilflingseder, Giuseppe Cappellano, Evi M. Morandi, Gerhard Pierer
Summary: The presence of adipocytes in wound tissue indicates a prevailing anti-inflammatory environment that can be promoted by NCT treatment, and may be associated with improved healing outcomes.
STEM CELL RESEARCH & THERAPY
(2021)
Article
Biochemistry & Molecular Biology
Katrin Radeloff, Dorothee Weiss, Rudolf Hagen, Norbert Kleinsasser, Andreas Radeloff
Summary: Adipose-derived stromal cells show chondrogenic differentiation capacity in vitro and have potential for cartilage replacement. Implantation of predifferentiated cell-seeded constructs into rabbit ear cartilage defects resulted in deposition of cartilage-like structures with evidence of endochondral ossification.
Article
Cell & Tissue Engineering
Richard J. P. Smith, Alessandro Faroni, James R. Barrow, Jamie Soul, Adam J. Reid
Summary: Enriching grafts with the CD271+ AD-MSC subpopulation has the potential to improve reconstructive and aesthetic surgeries involving adipose tissue, as these subpopulations express higher levels of angiogenic genes and lower levels of inflammatory genes, possess typical stem cell surface markers, and are more likely to promote effective neoangiogenesis than CD271- AD-MSCs.
STEM CELL RESEARCH & THERAPY
(2021)
Article
Pharmacology & Pharmacy
Prakash Gangadaran, Ramya Lakshmi Rajendran, Ji Min Oh, Eun Jung Oh, Chae Moon Hong, Ho Yun Chung, Jaetae Lee, Byeong-Cheol Ahn
Summary: The study found that extracellular vesicles (EVs) derived from stem cells isolated from human adipose tissue contain angiogenic proteins and promote angiogenesis in vitro and in vivo.
Article
Biochemistry & Molecular Biology
Mersedeh Tohidnezhad, Yusuke Kubo, Philipp Lichte, Tobias Heigl, Diana Roch, Nazanin Barahmand Pour, Christian Bergmann, Tolga Taha Soenmez, Jennifer Vanessa Phi Hock, Athanassios Fragoulis, Felix Gremse, Stefanie Rosenhain, Alexander Slowik, Michaela Bienert, Nisreen Kweider, Christoph Jan Wruck, Holger Jahr, Frank Hildebrand, Hans Christoph Pape, Sabine Neuss, Horst Fischer, Thomas Pufe
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2020)
Review
Biochemistry & Molecular Biology
Yu-Shan Chen, Wei-Shiung Lian, Chung-Wen Kuo, Huei-Jing Ke, Shao-Yu Wang, Pei-Chen Kuo, Holger Jahr, Feng-Sheng Wang
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2020)
Article
Cell Biology
Feng-Sheng Wang, Yu-Shan Chen, Jih-Yang Ko, Chung-Wen Kuo, Huei-Jing Ke, Chin-Kuei Hsieh, Shao-Yu Wang, Pei-Chen Kuo, Holger Jahr, Wei-Shiung Lian
Article
Biochemistry & Molecular Biology
Feng-Sheng Wang, Chung-Wen Kuo, Jih-Yang Ko, Yu-Shan Chen, Shao-Yu Wang, Huei-Jing Ke, Pei-Chen Kuo, Chin-Huei Lee, Jian-Ching Wu, Wen-Bin Lu, Ming-Hong Tai, Holger Jahr, Wei-Shiung Lian
Article
Biochemistry & Molecular Biology
Yusuke Kubo, Wolf Drescher, Athanassios Fragoulis, Mersedeh Tohidnezhad, Holger Jahr, Matthias Gatz, Arne Driessen, Jorg Eschweiler, Markus Tingart, Christoph Jan Wruck, Thomas Pufe
Summary: Osteonecrosis is a disease characterized by bone tissue death due to disturbance of the nutrient artery, with recent studies emphasizing the crucial role of oxidative stress in this process. However, current antioxidant monotherapy has not proven successful in preventing osteonecrosis in humans.
ANTIOXIDANTS & REDOX SIGNALING
(2021)
Review
Materials Science, Multidisciplinary
Holger Jahr, Yageng Li, Jie Zhou, Amir A. Zadpoor, Kai-Uwe Schroeder
Summary: Absorbable porous metals manufactured by additive manufacturing offer customizable multi-scale geometries, interconnected porous structure, and tunable absorption properties for bone regeneration and remodeling. Thorough characterization of their biodegradation behavior, mechanical properties, and bone regeneration ability is essential for developing ideal implants. In comparison to solid inert counterparts, AM absorbable porous metals possess unique properties and potential, but face challenges in future Orthopedic solutions.
FRONTIERS IN MATERIALS
(2021)
Article
Chemistry, Physical
Jannik Buehring, Maximilian Voshage, Johannes Henrich Schleifenbaum, Holger Jahr, Kai-Uwe Schroeder
Summary: AM porous scaffolds made of absorbable metals like magnesium, zinc, or iron, designed for orthopaedic applications, show decreasing Young's modulus over time due to product dissolution in a physiological environment. The initial increase in smeared Young's modulus for magnesium-based scaffolds is attributed to a forming substrate layer of degradation products. Even low thicknesses and Young's moduli of the substrate layer significantly increase the smeared Young's modulus under axial compression, as validated by compression tests on Zn1Mg AM scaffolds.
Article
Biochemistry & Molecular Biology
Yusuke Kubo, Rainer Beckmann, Athanassios Fragoulis, Claudius Conrads, Prathyusha Pavanram, Sven Nebelung, Michael Wolf, Christoph Jan Wruck, Holger Jahr, Thomas Pufe
Summary: Oxidative stress is associated with osteoarthritis, and the Nrf2/ARE signaling pathway controls SOX9 expression. Nrf2 directly regulates SOX9 in articular cartilage, and the loss of Nrf2 may lead to mild osteoarthritis in old age.
Article
Biochemistry & Molecular Biology
Holger Jahr, Anna E. van der Windt, Ufuk Tan Timur, Esther B. Baart, Wei-Shiung Lian, Bernd Rolauffs, Feng-Sheng Wang, Thomas Pufe
Summary: Increasing osmolarity to physiological levels promotes chondrogenic differentiation and marker expression in chondroprogenitor cells. The calcineurin inhibitor FK506 enhances this effect by modulating TGF-beta signaling at the type I receptor. Our findings provide insights into the mechanisms underlying cartilage repair strategies.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Biochemistry & Molecular Biology
Wei-Shiung Lian, Feng-Sheng Wang, Yu-Shan Chen, Ming-Hsien Tsai, How-Ran Chao, Holger Jahr, Re-Wen Wu, Jih-Yang Ko
Summary: Osteoporosis and osteoarthritis are the leading causes of musculoskeletal dysfunction in older adults. Gut microbiota and metabolites play an important role in maintaining the homeostasis of cartilage and bone tissue. Modulating gut microbiota and supplementing microbiota-derived metabolites may help slow down the development of these skeletal disorders.
Article
Engineering, Biomedical
Shuang Zhang, Bastiaan Tuk, Jeroen Van de Peppel, Gert-Jan Kremers, Marijke Koedam, Georg R. Pesch, Zaid Rahman, Remco M. Hoogenboezem, Eric M. J. Bindels, Johan W. Van Neck, Pouyan E. Boukany, Johannes P. T. M. Van Leeuwen, Bram C. J. Van der Eerden
Summary: A functional vascular system is crucial for bone repair, and disrupted angiogenesis can lead to non-union. Paracrine factors released by human bone marrow derived mesenchymal stromal cells (BM-SCs) have angiogenic effects on endothelial cells. This study used microfluidic designs to investigate angiogenesis and found that BMSC conditioned medium (CM) significantly promoted endothelial cell proliferation, chemotactic and mechanotactic migration. Transcriptional analysis of endothelial cells revealed unique gene expression related to tricarboxylic acid cycle and energy metabolism when exposed to both BMSC-CM and shear stress. This study highlights the importance of considering in vivo-like microenvironments when studying vessel repair during fracture healing.
ACTA BIOMATERIALIA
(2022)
Article
Cell Biology
Maximilian Koblenzer, Marek Weiler, Athanassios Fragoulis, Stephan Ruetten, Thomas Pufe, Holger Jahr
Summary: This study aimed to develop a bone mineralization model for more physiological high-throughput screenings. MC3T3-E1 pre-osteoblasts were cultured to form 3D spheroids, and the mineralization process was evaluated through gene expression, enzyme activity, and calcium deposition analyses. The established biomimetic model provides a versatile and cost-efficient tool for improved pharmacological ex vivo screenings.
Review
Biochemistry & Molecular Biology
Athanassios Fragoulis, Mersedeh Tohidnezhad, Yusuke Kubo, Christoph Jan Wruck, Rogerio Bastos Craveiro, Anna Bock, Michael Wolf, Thomas Pufe, Holger Jahr, Frank Suhr
Summary: Mechanosensing is crucial for maintaining tissue functions. Mechanical loading is necessary for the physiological functions of various tissues, while mechanical unloading can lead to pathological remodeling and dysfunctions. At the cellular level, both loading and unloading regulate cellular pathways, especially those involving oxidants like reactive oxygen species (ROS), which are essential for tissue organization and function.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Engineering, Biomedical
Y. Li, P. Pavanram, J. Buehring, S. Ruetten, K-U. Schroeder, J. Zhou, T. Pufe, L. N. Wang, A. A. Zadpoor, H. Jahr
Summary: This study investigates the performance of AM porous iron by comparing its biodegradation behavior and cytocompatibility under static and dynamic conditions. The research finds that the biodegradation rate of AM porous iron significantly increases under in situ-like conditions, and its compatibility with various cell types is enhanced.
ACTA BIOMATERIALIA
(2023)
Article
Materials Science, Biomaterials
Y. Li, P. Pavanram, J. Zhou, K. Lietaert, F. S. L. Bobbert, Yusuke Kubo, M. A. Leeflang, H. Jahr, A. A. Zadpoor
BIOMATERIALS SCIENCE
(2020)