Article
Computer Science, Interdisciplinary Applications
Yuqing Sun, Bo Zhang, Lianghua Xia
Summary: This study aimed to explore the morphological changes of endothelial cells under different levels of shear stress and find effective evaluation indicators through in vivo and in vitro experiments. The results showed that the size and duration of shear stress can affect the morphology and arrangement of endothelial cells. Traditional cell morphological indicators did not show significant statistical significance, while intercellular space characteristics could be used as effective indicators.
COMPUTER METHODS AND PROGRAMS IN BIOMEDICINE
(2021)
Review
Cell Biology
Shuo Feng, Jia Wei Chen, Xin Yi Shu, Muladili Aihemaiti, Jin Wei Quan, Lin Lu, Rui Yan Zhang, Chen Die Yang, Xiao Qun Wang
Summary: Hemodynamic shear stress (SS) regulates vascular homeostasis and injury through the release of endothelial microparticles (EMP), affecting physiological function of endothelial cells, inflammatory response, vascular permeability, and the coagulation system.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Multidisciplinary Sciences
Chak Kwong Cheng, Xiao Lin, Yujie Pu, Joyce Ka Yu Tse, Yu Wang, Cheng-Lin Zhang, Xiaoyun Cao, Chi Wai Lau, Juan Huang, Lei He, Jiang-Yun Luo, Yu-Tsung Shih, Song Wan, Chi Fai Ng, Li Wang, Ronald Ching Wan, Jeng-Jiann Chiu, Ting Fung Chan, Xiao Yu Tian, Yu Huang
Summary: This study utilized single-cell RNA sequencing to explore the transcriptional landscape and heterogeneity of dysfunctional endothelial cells in atherosclerotic aortae. SOX4 was identified as a novel marker for atherosclerosis and was shown to promote atherogenesis and endothelial-to-mesenchymal transition. Hyperlipidemia-associated cytokines and oscillatory blood flow were found to upregulate SOX4 expression, while the anti-diabetic drug metformin suppressed SOX4 levels in endothelial cells.
JOURNAL OF ADVANCED RESEARCH
(2023)
Review
Biochemistry & Molecular Biology
Siarhei A. Dabravolski, Alexander M. Markin, Elena R. Andreeva, Ilya I. Eremin, Alexander N. Orekhov, Alexandra A. Melnichenko
Summary: Pericytes play a key role in the pathogenesis and development of atherosclerosis, being involved in plaque formation and vulnerability. They can also be targeted as a therapeutic approach to promote vessel maturity and stability, reducing plaque vulnerability.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Multidisciplinary Sciences
Iguaracy Pinheiro-de-Sousa, Miriam H. Fonseca-Alaniz, Samantha K. Teixeira, Mariliza Rodrigues, Jose E. Krieger
Summary: This study examined the influence of different factors on endothelial dysfunction using global gene expression in human coronary artery endothelial cells, revealing a potential hierarchical interaction between surrogate risk factors of cardiovascular disease and the emergence of phenotypes affecting ED.
SCIENTIFIC REPORTS
(2022)
Review
Cell Biology
Mohammad Hamrangsekachaee, Ke Wen, Sidi A. Bencherif, Eno E. Ebong
Summary: Endothelium health is crucial for regulating physiological vascular functions. Dysfunction of endothelial cells (ECs) is associated with various vascular diseases, especially atherosclerosis and cardiovascular diseases. This review discusses mechanotransduction events mediated by ECs, the involved subcellular components, and potential pathways. It also reviews current research efforts using in vivo animal models and in vitro biomimetic models, along with recommendations for future modeling approaches to aid the development of novel therapies targeting atherosclerosis and related cardiovascular diseases.
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
(2023)
Article
Pharmacology & Pharmacy
Xiuying Li, Celine Souilhol, Lindsay Canham, Xueqi Jia, Mannekomba Diagbouga, Blanca Tardajos Ayllon, Jovana Serbanovic-Canic, Paul C. Evans
Summary: Flowing blood generates wall shear stress, which affects endothelial cell physiology and plays a crucial role in vascular development, homeostasis, and disease. Low oscillatory shear stress induces endothelial-to-mesenchymal transition (EndMT), which has divergent effects depending on the context. DLL4 is found to be essential for EndMT in the development of atrioventricular valves in embryos, and this study investigates its role in adult arteries.
VASCULAR PHARMACOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Min Wu, Yijin Wu, Shulin Tang, Jinsong Huang, Yueheng Wu
Summary: Single-cell RNA-seq analysis revealed the key molecular mechanisms of atherosclerosis, highlighting the impact of endothelial cells and the pathways of fluid shear stress and AS and TGF-beta. ICAM1, KLF2, and VCAM1 were identified as important genes in endothelial cells, with significantly downregulated expression in atherosclerotic arteries.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2023)
Review
Biochemistry & Molecular Biology
Shafiu A. Umar Shinge, Daifang Zhang, Ahmad Ud Din, FengXu Yu, YongMei Nie
Summary: This review highlights the role of mechanosensitive protein Piezo1 in atherosclerosis and its therapeutic potentials. Recent studies have shown that Piezo1 channel acts as a sensor and transducer of mechanical forces, affecting various cellular activities involved in atherosclerosis, such as proliferation, migration, apoptosis, and vascular remodeling. Understanding the mechanisms of Piezo1 and its drugability may lead to novel therapeutic strategies for treating atherosclerosis and other cardiovascular diseases.
INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES
(2022)
Article
Cell Biology
Johannes Jarausch, Lisa Neuenroth, Reiner Andag, Andreas Leha, Andreas Fischer, Abdul R. Asif, Christof Lenz, Abass Eidizadeh
Summary: This study investigated the effects of laminar shear stress, inflammation, and BETi on human endothelial cells using IMS-DIA-MS. The results showed significant changes in protein expression patterns due to inflammation, shear stress, and BETi treatment, with JQ1 having the greatest effect.
Review
Medicine, Research & Experimental
Hongxin Cheng, Wen Zhong, Lu Wang, Qing Zhang, Xiaoqing Ma, Yang Wang, Shiqi Wang, Chengqi He, Quan Wei, Chenying Fu
Summary: Different blood flow patterns in arteries alter the phenotype of vascular endothelial cells, affecting their functions and directly contributing to the occurrence of early atherosclerosis lesions. Oscillating shear stress (OSS) in curved or bifurcated arteries promotes the transformation of endothelial cells into pro-inflammatory phenotypes, leading to increased inflammation, oxidative stress, mitochondrial dysfunction, metabolic abnormalities, and enhanced endothelial permeability, thus promoting atherosclerosis progression. In contrast, stable laminar shear stress (LSS) in straight arteries facilitates the transformation of endothelial cells into an anti-inflammatory phenotype, improving their function and inhibiting atherosclerotic progression. This article outlines the relationship between vascular endothelial cell functions and different forms of fluid shear stress in atherosclerosis and explores potential therapeutic approaches targeting intracellular mechanical transductions.
BIOMEDICINE & PHARMACOTHERAPY
(2023)
Article
Biology
Darian Williams, Marwa Mahmoud, Renfa Liu, Aitor Andueza, Sandeep Kumar, Dong-Won Kang, Jiahui Zhang, Ian Tamargo, Nicolas Villa-Roel, Kyung-In Baek, Hwakyoung Lee, Yongjin An, Leran Zhang, Edward W. Tate, Pritha Bagchi, Jan Pohl, Laurent O. Mosnier, Eleftherios P. Diamandis, Koichiro Mihara, Morley D. Hollenberg, Zhifei Dai, Hanjoong Jo
Summary: Atherosclerosis primarily occurs in arterial regions exposed to disturbed blood flow, while regions exposed to stable flow are protected. The study discovered that KLK10, a protein sensitive to flow, is upregulated under stable flow conditions and downregulated under disturbed flow conditions. It acts as an anti-inflammatory, barrier-protective, and anti-atherogenic factor.
Review
Pharmacology & Pharmacy
Lei He, Cheng-Lin Zhang, Qinghua Chen, Li Wang, Yu Huang
Summary: Atherosclerotic vascular disease and its complications are leading causes of mortality globally. Studies have shown that endothelial cells are influenced by low or disturbed shear stress at curvatures and bifurcations in the arterial tree, playing a role in the formation and development of atherosclerotic plaques. Understanding mechanosensitive signaling cascades may offer new translational strategies for therapeutic intervention of atherosclerotic vascular disease.
PHARMACOLOGY & THERAPEUTICS
(2022)
Article
Cell Biology
Guozhang He, Shuang Kan, Shaohua Xu, Xuchen Sun, Rong Li, Wei Shu, Ming Chen
Summary: In this study, the researchers found that the expression of LXN in ECs is significantly influenced by laminar shear stress, and LXN knockdown can lead to changes in cell morphology and cytoskeleton remodeling, improving vascular damage and atherosclerosis in mice. This provides new strategies and drug targets for the treatment of vascular diseases.
JOURNAL OF CELLULAR AND MOLECULAR MEDICINE
(2021)
Article
Biochemistry & Molecular Biology
Maitha Aldokhayyil, Dulce H. Gomez, Marc D. Cook, Andreas N. Kavazis, Michael D. Roberts, Thangiah Geetha, Michael D. Brown
Summary: Tumor necrosis factor (TNF) binding to endothelial TNF receptor-I (TNFR-I) facilitates monocyte recruitment and chronic inflammation, leading to the development of atherosclerosis. In vitro data show a heightened inflammatory response and atherogenic potential in endothelial cells (ECs) from African American (AA) donors. High laminar shear stress (HSS) can mitigate some aspects of racial differences in endothelial function at the cellular level.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Biochemistry & Molecular Biology
Rio P. Juni, Kelly C. 't Hart, Riekelt H. Houtkooper, Reinier A. Boon
Summary: The advancement of medical technology has led to an increase in life expectancy but also a rise in aging-related diseases. Aging affects metabolism and cardiovascular health, and long noncoding RNAs (lncRNAs) play a crucial role in regulating metabolism and mitochondrial function. Dysregulation of lncRNAs can result in various metabolic disorders and cardiovascular consequences.
Article
Cell Biology
Veerle Kremer, Laura Stanicek, Eva van Ingen, Diewertje Bink, Sarah Hilderink, Anke J. Tijsen, Ilka Wittig, Lars Maegdefessel, Anne Yael Nossent, Reinier A. Boon
Summary: This study investigated the role and mechanism of the long non-coding RNA MEG8 in endothelial function. The results showed that MEG8 is induced during aging and has a protective effect. Knockdown of MEG8 impaired the endothelial barrier and affected the expression of specific miRNAs. MEG8 regulates the expression of mature miRNAs through interaction with CIRBP and HADHB.
JOURNAL OF CELL SCIENCE
(2022)
Article
Cardiac & Cardiovascular Systems
S. Engelhardt, S. Dimmeler, C. Heim, C. Baer, T. Boettger, R. Boon, R. P. Brandes, T. Braun, A. Dueck, J. Gagneur, P. Grote, L. M. Holdt, N. Jae, J. Krishnan, C. Kupatt, K. -l. Laugwitz, M. S. Leisegang, L. Maegdefessel, T. Meitinger, A. Moretti, M. Mueller-McNicoll, M. Sattler, A. Schnieke, M. Schulz, H. Schunkert, H. Schwalbe, D. Teupser, T. Thum, C. Weber, I. Wittig, K. Zarnack
Summary: The discovery of regulatory noncoding RNA molecules has revolutionized our understanding of gene expression. These RNA molecules, especially microRNAs, long noncoding RNAs, and circular RNAs, play important roles in the cardiovascular system and have potential for therapeutic applications. However, the complexity of RNA transcripts and the mechanisms of their actions present significant challenges for further research.
Article
Multidisciplinary Sciences
Anna Theresa Gimbel, Susanne Koziarek, Kosta Theodorou, Jana Felicitas Schulz, Laura Stanicek, Veerle Kremer, Tamer Ali, Stefan Guenther, Sandeep Kumar, Hanjoong Jo, Norbert Huebner, Lars Maegdefessel, Stefanie Dimmeler, Sebastiaan van Heesch, Reinier A. Boon
Summary: Despite the high expression and strong evolutionary conservation of TUG1, it does not seem to play a major role in basic endothelial cell function.
Article
Cardiac & Cardiovascular Systems
Mohammed Rabiul Hosen, Philip Roger Goody, Andreas Zietzer, Xu Xiang, Sven Thomas Niepmann, Alexander Sedaghat, Vedat Tiyerili, Ramesh Chennupati, Joseph B. Moore, Reinier A. Boon, Shizuka Uchida, Jan-Malte Sinning, Sebastian Zimmer, Eicke Latz, Nikos Werner, Georg Nickenig, Felix Jansen
Summary: This study found that increased levels of the circulating proapoptotic EV-incorporated miR-122-5p are associated with reduced cardiac function after TAVR. It was observed that miR-122-5p can be shuttled from endothelial cells into cardiomyocytes through large EVs. Mechanistically, miR-122-5p interacts with the RNA-binding protein hnRNPU and reduces the expression of the antiapoptotic gene BCL2 to decrease the viability of target cardiomyocytes.
Article
Genetics & Heredity
Josine M. de Winter, Karlijn Bouman, Joshua Strom, Mei Methawasin, Jan D. H. Jongbloed, Wilma van der Roest, Jan van Wijngaarden, Janneke Timmermans, Robin Nijveldt, Frederik van den Heuvel, Erik-Jan Kamsteeg, Baziel G. van Engelen, Ricardo Galli, Sylvia J. P. Bogaards, Reinier A. Boon, Robbert J. van der Pijl, Henk Granzier, Bobby Koeleman, Ahmad S. Amin, Jolanda van der Velden, J. Peter van Tintelen, Maarten P. van den Berg, Karin Y. Van Spaendonck-Zwarts, Nicol C. Voermans, Coen A. C. Ottenheijm
Summary: KBTBD13 variants are associated with cardiac dysfunction and cardiomyopathy, and NEM6 patients should be referred to a cardiologist.
Meeting Abstract
Cardiac & Cardiovascular Systems
Kennedy Silva Ramos, Jin Li, Reinier Boon, Mathijs van Schie, Natasja M. S. de Groot, Bianca Brundel
JOURNAL OF MOLECULAR AND CELLULAR CARDIOLOGY
(2022)
Meeting Abstract
Cardiac & Cardiovascular Systems
Rio Juni, Philippa Phelp, Diewertje Bink, Pedro Espinoza-Gonzales, Anke van Bergen, Diederik Kuster, Jolanda van der Velden, Paul Krijnen, Hans Niessen, Reinier Boon
JOURNAL OF MOLECULAR AND CELLULAR CARDIOLOGY
(2022)
Review
Cardiac & Cardiovascular Systems
Diewertje I. Bink, Jessica Pauli, Lars Maegdefessel, Reinier A. Boon
Summary: Atherosclerosis and other cardiovascular diseases are age-dependent. Endothelial cells play a crucial role in the development of atherosclerosis. Non-coding RNAs, such as microRNAs and long non-coding RNAs, have been identified to have a significant impact on endothelial function and disease progression. This article summarizes the altered expression of several microRNAs and long non-coding RNAs in endothelial aging and discusses their role in endothelial cell function and senescence. Targeting these non-coding RNAs controlling endothelial cell function and senescence may potentially attenuate atherosclerosis development.
Letter
Cardiac & Cardiovascular Systems
Rio P. Juni, Philippa G. Phelp, Diewertje I. Bink, Veerle Kremer, Anke S. van Bergen, Pedro Espinosa Gonzalez, Karlijn B. Rombouts, Karl J. Harber, Daan A. F. Heister, Kak K. Yeung, Jan van den Bossche, Diederik W. D. Kuster, Paul A. J. Krijnen, Hans W. M. Niessen, Reinier A. Boon
CIRCULATION RESEARCH
(2023)
Article
Neurosciences
Elisa Weiss, Anna Schruefer, Carolina Tocantins, Mariana Simoes Diniz, Boris Novakovic, Anke S. van Bergen, Azra Kulovic-Sissawo, Richard Saffery, Reinier. A. A. Boon, Ursula Hiden
Summary: High gestational weight gain (GWG) is associated with impaired wound healing of neonatal endothelial colony forming cells (ECFCs). A specific long non-coding RNA (lncRNA), KLRK1-AS1, was found to be downregulated in neonatal ECFCs from pregnancies with higher GWG, and its knockdown resulted in further impairment of barrier recovery. These findings suggest a potential role for lncRNAs, particularly KLRK1-AS1, in regulating neonatal ECFC function and may provide insights into the link between GWG and cardiovascular risk factors.
JOURNAL OF PHYSIOLOGY-LONDON
(2023)
Article
Cardiac & Cardiovascular Systems
Frederike Boos, James A. Oo, Timothy Warwick, Stefan Guenther, Judit Izquierdo Ponce, Melina Lopez, Diba Rafii, Giulia Buchmann, Minh Duc Pham, Zahraa S. Msheik, Tianfu Li, Sandra Seredinski, Shaza Haydar, Sepide Kashefiolasl, Karl H. Plate, Ruediger Behr, Matthias Mietsch, Jaya Krishnan, Soni S. Pullamsetti, Sofia-Iris Bibli, Rabea Hinkel, Andrew H. Baker, Reinier A. Boon, Marcel H. Schulz, Ilka Wittig, Francis J. Miller, Ralf P. Brandes, Matthias S. Leisegang
Summary: Long non-coding RNA LINC00607 is highly enriched in human endothelial cells and plays a regulatory role in angiogenesis by interacting with chromatin remodeler BRG1 to maintain ERG target gene transcription. Knockdown or knockout of LINC00607 attenuates VEGF-A-induced angiogenic sprouting and integration into vascular networks. Overexpression of LINC00607 restores normal endothelial function. These findings provide new insights into the role of lncRNAs in endothelial cells and their potential implications in cardiovascular disease.
BASIC RESEARCH IN CARDIOLOGY
(2023)
Article
Obstetrics & Gynecology
Veerle Kremer, Jetta J. Oppelaar, Theresa Gimbel, Susanne Koziarek, Wessel Ganzevoort, Marielle G. van Pampus, Bert-Jan Van den Born, Liffert Vogt, Christianne de Groot, Reinier A. Boon
Summary: This study found that NOVA2 regulates FLT1 splicing in endothelial cells, and the loss of NOVA2 results in impaired endothelial function, partially dependent on VEGF. A negative correlation between NOVA2 and sFLT1 was observed in pre-eclampsia patients.
REPRODUCTIVE SCIENCES
(2023)
Meeting Abstract
Cardiac & Cardiovascular Systems
C. Kohnle, K. Theodorou, S. Koziarek, J. Sommer, D. Busscher, J. U. G. Wagner, I. Wittig, S. Dimmeler, R. A. Boon
EUROPEAN HEART JOURNAL
(2022)
Meeting Abstract
Cardiac & Cardiovascular Systems
D. I. Bink, T. P. Pham, A. Van Bergen, L. Stanicek, D. Van der Ven, E. M. J. Groenen, L. Kaagman, M. Jonk, P. Hofmann, S. Dimmeler, R. A. Boon
EUROPEAN HEART JOURNAL
(2022)