Article
Chemistry, Multidisciplinary
Qingyu Ye, Xinzhao Xu, Alessandro Paghi, Thomas Bamford, Benjamin R. Horrocks, Andrew Houlton, Giuseppe Barillaro, Stoichko Dimitrov, Matteo Palma
Summary: In this study, the formation of carbon nanotube (CNT)-based nanohybrids in aqueous solution is reported, where DNA-wrapped CNTs act as templates for PbS and CdS nanocrystals growth. Multiplexed photoresponsive devices with sensitivity to various illumination wavelengths were fabricated from these nanohybrids. The different photoresponses of the DNA-CNT, CdS-DNA-CNT, and PbS-DNA-CNT devices were attributed to varying operational mechanisms, with PbS-DNA-CNT devices showing the fastest response due to photoinduced charge transfer.
ADVANCED FUNCTIONAL MATERIALS
(2021)
Article
Biophysics
Samet Kocabey, Guillermo P. Acuna, Curzio Ruegg
Summary: miRNA has the potential to be biomarkers for early disease detection, and the sensitive and accurate detection of miRNA can improve diagnosis and treatment of diseases, especially cancer. This study introduces a DNA origami nanoarray system that uses super-resolution microscopy technique DNA-PAINT to detect miRNAs based on their distance to boundary markers. The sensor can detect up to 4 miRNAs separately or in combination, with high sensitivity and large dynamic range, without the need for amplification. The detection system can also discriminate single base mismatches with low false positive rates. This ultrasensitive and amplification-free miRNA detection method has been successfully applied to detect breast-cancer associated miRNAs in cell extracts and plasma.
BIOSENSORS & BIOELECTRONICS
(2023)
Review
Chemistry, Analytical
Benhui Dai, Ruiyun Zhou, Jianfeng Ping, Yibin Ying, Lijuan Xie
Summary: This article discusses the use of carbon nanotubes (CNTs) in biosensors to improve their performance. The unique properties of CNTs, such as larger surface area and faster electron transfer, enhance the sensitivity and efficiency of biosensors. The article provides detailed descriptions of CNT-based sensors for detecting glucose, DNA, proteins, amino acids, and neurotransmitters. Continuous improvement in materials and structures can further enhance the detection performance of these sensors.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Physical
Qinqi Ren, Yaping Zhang, Shenhui Ma, Xiaofang Wang, Kuan-Chang Chang, Yiming Zhang, Feng Yin, Zigang Li, Min Zhang
Summary: This study proposes a low temperature supercritical carbon dioxide fluid activation method to enhance the performance of transistor-based biosensors by repairing dangling bonds and passivating hydroxyl groups. Using AKT2 gene as an example, the biosensors modified by this method show highly sensitive and consistent response to triple-negative breast cancer.
Article
Chemistry, Multidisciplinary
Sara Bracaglia, Simona Ranallo, Kevin W. Plaxco, Francesco Ricci
Summary: The electrochemical DNA circuit designed for detecting specific antibodies is sensitive, specific, and selective, suitable for use in complex media. This versatile circuit can detect multiple different antibodies and conduct multiplexed detection without crosstalk.
Article
Biophysics
Shenhui Ma, Yaping Zhang, Qinqi Ren, Xiaofang Wang, Jiahao Zhu, Feng Yin, Zigang Li, Min Zhang
Summary: The study introduces a method for label-free DNA detection using carbon nanotube-based biosensors, achieving successful detection of AKT2 gene related to triple-negative breast cancer with high sensitivity and selectivity. The platform has great potential for multiplexed detection of various cancer biomarkers and provides a universal strategy for low-cost clinical applications.
BIOSENSORS & BIOELECTRONICS
(2022)
Article
Chemistry, Multidisciplinary
Yu Zhao, Zhihao Li, Bo Li, Chunyan Wang
Summary: Accurate cancer diagnosis, especially early diagnosis, is crucial for prompt therapy and increased survival rate. mRNAs are widely used as biomarkers for cancer identification and treatment. mRNA expression levels are closely associated with cancer stage and malignant progression. However, single type mRNA detection is insufficient and unreliable. In this paper, a DNA nano-windmill probe was developed for in situ multiplexed mRNAs detection and imaging. The probe targets four types of mRNA simultaneously through wind blades, enabling independent recognition and cell type discrimination. The probe can specifically distinguish cancer cell lines from normal cells and identify changes in mRNA expression levels in living cells. This strategy enriches the toolbox for improving the accuracy of cancer diagnosis and therapeutic solutions.
CHEMISTRY-A EUROPEAN JOURNAL
(2023)
Article
Nanoscience & Nanotechnology
Melika Shahhosseini, Peter E. Beshay, Ehsan Akbari, Niksa Roki, Christopher R. Lucas, Alex Avendano, Jonathan W. Song, Carlos E. Castro
Summary: This study presents a method to monitor the interactions between cells and molecules in their surrounding environment in a three-dimensional tissue model. By utilizing a DNA origami cell sensing platform, specific nucleic acid sequences on the cell membrane were detected in real-time, and biomolecules on the cell surface were also detected in a 3D microfluidic tissue model.
ACS APPLIED MATERIALS & INTERFACES
(2022)
Article
Biophysics
Soochul Shin, Sun Han, Juyoung Kim, Yumi Shin, Ji-Joon Song, Sungchul Hohng
Summary: SIMUL is a multiplexed ctDNA detection technique that can sensitively and specifically detect multiple single-nucleotide mutations in early cancer stages. It accurately measures mutant fractions and holds immense potential for clinical applications.
BIOSENSORS & BIOELECTRONICS
(2023)
Article
Multidisciplinary Sciences
Shenglin Cai, Thomas Pataillot-Meakin, Akifumi Shibakawa, Ren Ren, Charlotte L. Bevan, Sylvain Ladame, Aleksandar P. Ivanov, Joshua B. Edel
Summary: The study presents a new sensing platform for miRNA detection, offering high sensitivity and specificity directly in human serum, and demonstrating monitoring of miR-141-3p and miR-375-3p in various stages of prostate cancer.
NATURE COMMUNICATIONS
(2021)
Article
Biophysics
Murali Mohana Rao Singuru, Yu-Chieh Liao, Gloria Meng-Hsuan Lin, Wei-Tzu Chen, Yu-Hsuan Lin, Ching Tat To, Wei-Ching Liao, Chun-Hua Hsu, Min-Chieh Chuang
Summary: In this paper, DNA-assembly-based capsules that can detect multiple genotoxic agents simultaneously are presented. The DNA sequences in the capsules are designed to exhibit multivalent susceptibility against different genotoxicants. The DNA capsules dissociate upon exposure to genotoxicants, releasing fluorophores for measurement of the genotoxicant level. The capsules allow monitoring of human exposure to genotoxic agents and facilitate timely remedial measures.
BIOSENSORS & BIOELECTRONICS
(2022)
Article
Multidisciplinary Sciences
Yingfeng Tao, Xiaoliu Zhou, Leqiang Sun, Da Lin, Huaiyuan Cai, Xi Chen, Wei Zhou, Bing Yang, Zhe Hu, Jing Yu, Jing Zhang, Xiaoqing Yang, Fang Yang, Bang Shen, Wenbao Qi, Zhenfang Fu, Jinxia Dai, Gang Cao
Summary: In this study, the authors developed a versatile pi-FISH rainbow method that can efficiently detect a range of biomolecules, including DNA, RNA, proteins, and neurotransmitters. They successfully applied this method to detect gene expression in different species and decoded the spatial distribution of neuron subclusters using only two rounds of hybridization. Furthermore, they combined pi-FISH rainbow with hybridization chain reaction to develop pi-FISH+ technology for detecting short nucleic acid fragments in circulating tumor cells.
NATURE COMMUNICATIONS
(2023)
Article
Chemistry, Analytical
Jiayu Yan, Zhichen Xu, Hu Zhou, Tao Li, Xincheng Du, Rui Hu, Jiang Zhu, Gaozhi Ou, Ying Li, Yunhuang Yang
Summary: A new detection platform called CasGDP based on CRISPR/Cas12a and multiplexed RPA has been proposed for rapid evaluation of gene doping. It showed high specificity and low detection limits, successfully established a GD cell model with the hEPO gene, and efficiently measured trace amounts of EPO transgene in human serum in 40 minutes using fluorescence and LFD.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Analytical
Yan Huang, Zhihao Cheng, Li-Ping Xu, Xueji Zhang, Guodong Liu
Summary: We developed a novel lateral flow DNA biosensor based on carbon nanotubes and triple helix DNA. This biosensor can detect DNA with high sensitivity and has the potential for applications in qualitative and quantitative analysis. It has significant implications for clinical diagnosis and biomedical research.
ANALYTICA CHIMICA ACTA
(2023)
Article
Multidisciplinary Sciences
Ren Ren, Shenglin Cai, Xiaona Fang, Xiaoyi Wang, Zheng Zhang, Micol Damiani, Charlotte Hudlerova, Annachiara Rosa, Joshua Hope, Nicola J. Cook, Peter Gorelkin, Alexander Erofeev, Pavel Novak, Anjna Badhan, Michael Crone, Paul Freemont, Graham P. Taylor, Longhua Tang, Christopher Edwards, Andrew Shevchuk, Peter Cherepanov, Zhaofeng Luo, Weihong Tan, Yuri Korchev, Aleksandar P. Ivanov, Joshua B. Edel
Summary: We present a single-molecule nanopore sensing approach combined with position-encoded DNA molecular probes, which can simultaneously identify various antigen proteins and multiple RNA gene fragments of SARS-CoV-2 with high sensitivity and selectivity. This strategy enables direct detection of spike (S) and nucleocapsid (N) proteins in unprocessed human saliva as well as the identification of RNA fragments from patient samples using nasal/throat swabs, allowing for the detection of critical mutations among viral variants. The sensing strategy can detect and distinguish between different SARS-CoV-2 lineages without the need for nucleic acid sequencing.
NATURE COMMUNICATIONS
(2023)
Article
Chemistry, Analytical
Jia Chen, Xin Wei, Hao Tang, Jean Claude Munyemana, Ming Guan, Shusheng Zhang, Hongdeng Qiu
Summary: The study investigated the peroxidase catalytic activity of ZnCo2O4 nanosheets synthesized in a deep eutectic solvent, showing that PPi inhibits and PPase restores the activity. This paves the way for colorimetric analysis of PPi and PPase.
Review
Chemistry, Analytical
Jia Chen, Zhengjun Gong, Weiyang Tang, Kyung Ho Row, Hongdeng Qiu
Summary: Carbon dots (CDs) are a new class of zero-dimensional carbon-based nanomaterials that have gained significant interest and are widely used in sensors, nanomedicine, bioimaging, and catalysis. They offer advantages of economy, low toxicity, and abundant carbon source, providing exciting opportunities for future research.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2021)
Review
Chemistry, Analytical
Jean Claude Munyemana, Jia Chen, Yangxia Han, Shusheng Zhang, Hongdeng Qiu
Summary: LDHs have been extensively researched for their biocompatibility and ion exchange properties, leading to the development of sensing nanoplatforms with enhanced selectivity and sensitivity. Recent studies have shown promising results in exploring LDHs as sensors for various analytes, particularly in optical sensing strategies utilizing their unique properties. Research in this area has shown potential for future advancements in designing improved sensors based on LDHs.
Article
Chemistry, Medicinal
Guoying Luo, Lanlan Sun, Heng Li, Jia Chen, Peilan He, Liang Zhao, Wei Tang, Hongdeng Qiu
Summary: Radiotherapy is a key modality for cancer treatment but can cause radiotoxicity on healthy cells. The newly synthesized NITR spin-labeled RES derivatives can significantly alleviate damage to Jurkat T cells induced by ionizing radiation, protecting the cells from injury.
Article
Chemistry, Multidisciplinary
Jing Xiao, Jia Chen, Hongdeng Qiu
Summary: Covalent organic frameworks (COFs) have gained significant attention as an emerging crystalline porous material. This study successfully synthesized four COFs with different morphologies using a green reaction medium composed of polyethylene glycol (PEG) and water. The researchers also investigated the versatility of this reaction medium in COF synthesis and explored the mechanism by which it modulates COF morphology. The findings suggest a promising approach for facile and environmentally friendly COF synthesis.
Article
Chemistry, Multidisciplinary
Yu Wang, Jia Chen, Ming Guan, Hongdeng Qiu
Summary: A novel Fe/Ni-PG material was prepared for effectively removing sulfonamides in aqueous solution, demonstrating excellent adsorption performance. The study also investigated the adsorption mechanism and influencing factors.
Article
Chemistry, Analytical
Danni Jiang, Jia Chen, Ming Guan, Hongdeng Qiu
Summary: In this study, 1-vinyl-3-octadecylimidazolium bromide ionic liquids and their derived carbon dots were prepared and grafted on silica to obtain enhanced separation performance for various analytes. The co-grafted column showed superior separation performance and was successfully applied to determine the contents of specific compounds in Radix Astragali extract.
Review
Chemistry, Analytical
Yu Wang, Jia Chen, Hirotaka Ihara, Ming Guan, Hongdeng Qiu
Summary: Porous carbon nanomaterials are favored by researchers for their excellent properties and have been widely used in various fields. New sample preparation methods based on PCNMs have emerged, showing great potential in solid phase extraction techniques.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Multidisciplinary
Jean Claude Munyemana, Jia Chen, Yun Liu, Yongpeng Wang, Hongdeng Qiu
Summary: This study demonstrates the synthesis of cerium-zinc oxide and cerium-zinc hydroxide nitrate using a deep eutectic solvent, showing catalytic activity towards dopamine polymerization and potential for pyrophosphate ion detection strategies. The proposed strategy offers a novel green approach for preparing hierarchically shaped materials.
ACS SUSTAINABLE CHEMISTRY & ENGINEERING
(2021)
Article
Chemistry, Multidisciplinary
Li Liang, Jia Chen, Xuwei Chen, Jianhua Wang, Hongdeng Qiu
Summary: A novel graphene oxide/COFs composite with favorable adsorption and regeneration capabilities has been synthesized in this study, which is expected to play an important role in environmental remediation.
ENVIRONMENTAL SCIENCE-NANO
(2022)
Article
Chemistry, Analytical
Yun Liu, Xing Wei, Jia Chen, Yong-Liang Yu, Jian-Hua Wang, Hongdeng Qiu
Summary: CeO2-Co(OH)2 nanosheets were synthesized in a newly designed deep eutectic solvent, and a colorimetric assay was developed for the quantitative detection of AChE and screening of inhibitors. The CeO2-Co(OH)2 nanosheets showed excellent oxidase-like activity and could be used for the diagnosis and therapy of neurological diseases.
ANALYTICAL CHEMISTRY
(2022)
Article
Biochemical Research Methods
Chao Fan, Jia Chen, Hui Li, Kaijun Quan, Hongdeng Qiu
Summary: Two new hydrophilic-hydrophobic and acid-base balanced liquid chromatographic stationary phases were developed using polymer-functionalized silica design strategy. These stationary phases can separate compounds with different properties in reversed-phase liquid chromatography and hydrophilic interaction liquid chromatography modes.
JOURNAL OF CHROMATOGRAPHY A
(2022)
Article
Chemistry, Analytical
Yuting Wang, Jia Chen, Ganping Wang, Yongliang Yu, Jianhua Wang, Hongdeng Qiu
Summary: This study presents a facile and controllable approach for synthesizing high-performance fluorescent covalent organic frameworks (COFs). The influence of acetic acid and reaction time on the morphology and properties of COFs is explored. Flower-shaped COFs are successfully prepared and used for highly sensitive detection of Cys and Hcy.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Article
Chemistry, Analytical
Weiyang Tang, Yena An, Jia Chen, Kyung Ho Row
Summary: This paper presents a biosensing platform based on CuPd@H-C3N4 composite material, which exhibits peroxidase, oxidase, and catalase-like activities. The platform allows for visual and highly sensitive detection of glutathione and glucose, and also proposes a simplified glucose detection strategy.
Article
Biochemistry & Molecular Biology
Pascaline Sanga, Juanjuan Wang, Xin Li, Jia Chen, Hongdeng Qiu
Summary: This study presents the preparation and utilization of novel BiFeO3/MXene nanocomposites for the removal of sulfonamides from water. The BiFeO3/MXene composites exhibit enhanced adsorption capabilities compared to pristine MXene due to the synergistic effect between BiFeO3 and MXene. The adsorption kinetics and isotherm models are consistent with pseudo-second-order kinetics and Langmuir model, respectively. BiFeO3/MXene also shows good reusability and stability after multiple adsorption-desorption cycles.
Article
Chemistry, Analytical
Qian Wu, Li Tian, Xiangyu Shan, Huiling Li, Chao Li, Juan Lu
Summary: A novel ECL sensor was developed by modifying Fe3CuO4-Ru(bpy)3+ and GO-CdS@ZnS on the electrode for the detection of permethrin. The sensor exhibited high sensitivity and a wide detection range.
Article
Chemistry, Analytical
Tingting Gong, Lei Liao, Bingying Jiang, Ruo Yuan, Yun Xiang
Summary: In this study, a fluorescent biosensing system for high sensitivity detection of MT was established using a new Ag+-stabilized DNA triplex probe (Ag+-SDTP) combined with catalytic hairpin assembly (CHA) and CRISPR/Cas12a signal enhancements. The sensing method is able to selectively detect MT from other non-specific molecules and can achieve low level detection of MT in diluted human serums, demonstrating its potential for monitoring disease-specific MT biomarker at trace levels.
Article
Chemistry, Analytical
Estrella Sanz Rodriguez, Kyra Wood, Paul R. Haddad, Brett Paull
Summary: This study presents a simple, rapid, and reliable method for determining boron in aqueous solutions using suppressed ion chromatography coupled to electrospray ionisation-triple quadrupole mass spectrometry. The method achieved a low limit of detection and demonstrated excellent analytical performance.
Article
Chemistry, Analytical
Aline Cournut, Paul Moustiez, Yannick Coffinier, Christine Enjalbal, Claudia Bich
Summary: Alzheimer's disease is a major cause of senile dementia and is characterized by the aggregation of neurofibrillary tangles and amyloid plaques in the brain. The A beta 1-42 peptide is considered the primary cause of neurotoxicity due to its insolubility and subsequent aggregation. A beta 1-42 and A beta 1-40 have been identified as relevant biomarkers for AD diagnosis, and SurfaceAssisted Laser Desorption/Ionization Mass Spectrometry (SALDI-MS) was used to monitor the A beta 1-42/A beta 1-40 ratio without prior sample treatment.
Article
Chemistry, Analytical
Dotse Selali Chormey, Buse Tugba Zaman, Tulay Borahan Kustanto, Sezin Erarpat Bodur, Suleyman Bodur, Elif Ozturk Er, Sezgin Bakirdere
Summary: This review provides an overview of the harmful effects of endocrine disrupting chemicals (EDCs) on human health and introduces the methods for analyzing EDCs. Recent research has focused on using green chemicals, such as deep eutectic solvents (DESs), for microextraction to ensure environmental safety.
Article
Chemistry, Analytical
Yan Zhang, Renfeng Xu, Jingjing Wu, Zhenghong Zhang, Yuhuang Wang, Hongqin Yang, Sheng Zhang
Summary: This study visualized the pore-like structures in prostate cancer cell membranes after treatment with the anticancer drug paclitaxel, demonstrating its mechanism of action related to oxidative damage.
Article
Chemistry, Analytical
Chihong Ma, Qin Zhou, Jinjin Shi, Hua Gao, Di Huang, Huimin Xue, Han Wang, Zhenzhong Zhang, Sen Yang, Junli Zhang, Kaixiang Zhang
Summary: We developed a CRISPR-empowered electrochemical biosensor, named PER-E-CRISPR, for amplification-free and sensitive detection of miR-21. The biosensor combines the advantages of CRISPR/Cas13a and primer exchange reaction (PER) for target amplification. Under optimized conditions, the PER-E-CRISPR assay exhibits a linear range from 10-13 to 10-7 M and a limit of detection of 30.2 fM. The biosensor shows excellent performance in actual plasma, indicating promising prospects for miRNA detection in molecular diagnosis.
Article
Chemistry, Analytical
Gang Liang, Jie Zhao, Yufei Gao, Tao Xie, Jianhui Zhen, Ligang Pan, Wenwen Gong
Summary: Molecular docking (MD) analysis is widely used for studying the interaction between aptamers and small molecules. This study established steady-state aptamers of tertiary structures (SATS) using specific aptamers of tetracycline antibiotics as docking models. Molecular docking results revealed multiple binding sites in the SATS of aptamers, with significant variations in binding free energy (BFE) and docking score (DS). The study also proposed a method for MD analysis based on SATS, providing insights into binding mode and predicting binding sites.
Article
Chemistry, Analytical
Yixuan Xu, Changchang Teng, Huiping Dang, Dalong Yin, Lifeng Yan
Summary: Stable organic radical molecules with high NIR fluorescence quantum efficiency have been synthesized and encapsulated into nanoparticles, which show great potential for both photodynamic therapy (PDT) and photothermal therapy (PTT) of tumors.
Article
Chemistry, Analytical
Yu Su, Dandan Yang, Yanjie Wang, Jie Ding, Lan Ding, Daqian Song
Summary: In this study, a highly selective molecularly imprinted polymer Cu-MIPs@CS was designed and synthesized for bisphenol A (BPA). Cu-MIPs@CS showed significantly enhanced imprinting factor and selective factor towards BPA compared to MIPs@CS. It also exhibited superior discrimination ability between BPA and its structural analogue. Additionally, Cu-MIPs@CS were successfully applied as a solid phase extraction adsorbent for the determination of BPA in drinking water samples.
Article
Chemistry, Analytical
Yi Qiao, Qiongdan Zhang, Yukun He, Tianguang Cheng, Jing Tu
Summary: Single cell heterogeneity plays an important role in many biological phenomena, and distinguishing cells with specific mutations is beneficial for clinical diagnosis and drug screening. This study introduces a gel plate platform that can distinguish cell types based on their phenotypes, with low equipment requirement. The gel platform has potential in point-of-care circumstances and single-cell stimulation responses that prioritize efficiency and simplicity.
Article
Chemistry, Analytical
Chengke Wang, Shuyang Sun, Ping Wang, Huawei Zhao, Wenling Li
Summary: The food safety of aquatic products is a global concern. Nanotechnology-based analyses have advantages in the detection of bacteria, metal ions, and small molecule contaminants. This review summarizes the recent advances in biosensing strategies for aquatic products and highlights the application of nanomaterials, lateral flow-based biosensors, surface-enhanced Raman scattering, microfluidic chips, and molecular imprinting technologies.
Article
Chemistry, Analytical
Liping Zhu, Zeng Tang, Xuemei Zhang, Li Zhu, Tian Meng, Linying Yu, Ting Xiao, Shasha Lu, Xiaoli Xiong, Xiurong Yang
Summary: A sensitive off-on electrochemiluminescence DNA sensor was developed using Exo III-assisted cascade amplification system. The sensor achieved high selectivity and ultra-high sensitivity in detecting target DNA by utilizing ECL and SPR effects. This study provides a new perspective for designing highly sensitive and programmable ECL biosensors.
Article
Chemistry, Analytical
Tong Li, Mengxu Sun, Suping Xia, Ting Huang, Rong-Tian Li, Chunrong Li, Zong Dai, Jin-Xiang Chen, Jun Chen, Nuan Jia
Summary: Here, we developed a binary system based on DNA tetrahedron and fluorogenic RNA aptamers for highly specific and label-free mRNA imaging in living cells. This system achieved high specificity label-free detection of nucleic acids with a detection limit of 1.34 nM. The method has potential applications in early disease diagnosis and new drug development.
Article
Chemistry, Analytical
Yao Zhao, Shu Zhang, Wang Yao, Yuxuan Zhu, Jing Qian, Juan Yang, Nianjun Yang
Summary: A new hierarchical nanostructured composite with high conductivity and catalytic activity is synthesized. The sensor exhibits significant catalytic effect and high sensitivity for monitoring the environmental endocrine disruptor bisphenol A (BPA).
