Article
Chemistry, Analytical
Lucas de Brito Ayres, University Jordan Brooks, Kristi Whitehead, Carlos D. Garcia
Summary: Several groups have explored the development of electrochemical paper-based wearable devices for detecting metabolites in sweat. However, there are challenges that need to be addressed before these sensors can be widely used in clinical interventions. This study presents a wearable electrochemical biosensor using paper-derived carbon electrodes, sputtered gold, and chitosan, which has the potential to detect Staphylococcus aureus.
ANALYTICAL CHEMISTRY
(2022)
Article
Nanoscience & Nanotechnology
Eduardo Aznar-Gadea, Pedro J. Rodriguez-Canto, Sandra Albert Sanchez, Juan P. Martinez-Pastor, Rafael Abargues
Summary: This study reports a luminescent chemosensor based on CdSe quantum dots embedded in polycaprolactone, which exhibits a fast response time and high sensitivity for the detection and identification of explosive taggants.
ACS APPLIED NANO MATERIALS
(2022)
Article
Chemistry, Analytical
Rui Liu, Suleiman A. Haruna, Shujat Ali, Jing Xu, Yunlian Zhang, Peng Lu, Huanhuan Li, Quansheng Chen
Summary: A fluorescent genosensor was developed for Staphylococcus aureus detection, utilizing interactions between specific single-stranded DNA and graphene oxide quantum dots for sensitive detection. The sensor exhibited high sensitivity with a LOD of 0.98 x 10(-17) mol L-1 and was successfully applied for real sample analysis. Additionally, the sensor could be adapted for the detection of other pathogens by replacing recognition probes and primers.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Article
Immunology
Jiewei Cui, Minjuan Zhou, Ying Li, Zhixin Liang, Yanqin Li, Ling Yu, Yang Liu, Yuan Liang, Liangan Chen, Changxi Yang
Summary: A quantum dots immunofluorescence biosensor was successfully developed for S. aureus detection, demonstrating high specificity and sensitivity. The biosensor has a low detection limit and can effectively distinguish other pathogens.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Biophysics
Yong Zhao, Yanzhao Li, Pingping Zhang, Ziheng Yan, Yugui Zhou, Yipu Du, Chunyu Qu, Yajun Song, Ding Zhou, Songnan Qu, Ruifu Yang
Summary: The study developed a highly sensitive fluorescent immunosensor using CDs-microspheres for the specific detection of Escherichia coli O157:H7 in milk, showing strong fluorescence intensity, good stability, and high uniformity in fluorescence. The immunosensor had a detection limit of 2.4 x 10(2) CFU/mL and could detect spiked E. coli O157:H7 in milk within 30 minutes with good repeatability. Additionally, the method could detect trace amounts of E. coli O157:H7 in real milk samples with 100% accuracy after bacterial enrichment, demonstrating its potential as a rapid and sensitive tool for pathogen detection in milk and other foods.
BIOSENSORS & BIOELECTRONICS
(2021)
Article
Microbiology
Pengfei Heng, Jiakai Liu, Zhen Song, Chuan Wu, Xiuzhong Yu, Yang He
Summary: In this study, a rapid and easy-to-operate detection method for Staphylococcus aureus was developed. The assay showed high specificity and sensitivity, able to detect S. aureus in only 25 minutes with a lower limit of detection compared to traditional PCR. The method also demonstrated high accuracy in detecting S. aureus in various bacterial samples, making it useful for timely diagnosis and prevention of S. aureus infection.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Chemistry, Analytical
Shu-Han Chang, Hanene Salmi-Mani, Philippe Roger, Sue-min Chang
Summary: A fluorescent microgel for BPA detection was developed by crosslinking poly(styrene-co-glycidyl methacrylate) with CdSe quantum dots. The microgel demonstrated high sensitivity and selectivity towards BPA molecules through specific binding sites. It had a wide dynamic detection range and low detection limit, and outperformed other structural analogues.
Article
Chemistry, Physical
Arun Ashokan, Paul Mulvaney
Summary: Solution-phase spectroelectrochemistry was used to study electron injection into colloidal CdSe quantum dots in THF. The results showed reversible optical changes on a time-scale of minutes, with the injection of electrons leading to quenching of photoluminescence. The number of electrons injected into the QDs depended on the electrode potential and QD size, with most injection occurring below the band edge.
CHEMISTRY OF MATERIALS
(2021)
Article
Chemistry, Analytical
Hongliang Wang, Dan Song, Yue Zhou, Jiayuan Liu, Anna Zhu, Feng Long
Summary: The significant fluorescence enhancement of CdSe/ZnS quantum dots induced by Hg2+ was observed for the first time using a CdSe/ZnS QD-modified fiber nanoprobe. The fluorescence enhancement mechanism was attributed to Zn-to-Hg cation exchange in the ZnS shell, forming a HgxZn1-xS/CdSe heterojunction. The maximum fluorescence intensity increased over eight times and the characteristic time was linearly dependent on Hg2+ concentration.
Article
Spectroscopy
Zhiqiang Zhou, Jianfang Cen, Naijia Jiang, Yu Sun, Zhouyang Li, Liyun Yang
Summary: In this paper, a novel ratiometric fluorescence sensor using CdSe quantum dots (QDs) was constructed for sensitive and selective detection of Ag+. The sensor is based on the formation of Ag2Se QDs. The detection limit of the sensor is 1.4 nM and it can be successfully applied to detect Ag+ in real water samples and living cells.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2023)
Article
Agriculture, Dairy & Animal Science
Shuangyan Ding, Hailiang Hu, Xianglin Yue, Kaiwen Feng, Xiaoyu Gao, Qiuling Dong, Mingqi Yang, Ugur Tamer, Ganhui Huang, Jinsheng Zhang
Summary: A new detection method based on quantum dots was developed to detect Salmonella in milk, with a detection limit 10 times better than conventional sandwich ELISA. The biosensor showed good specificity for detecting Salmonella.
JOURNAL OF DAIRY SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Yeseul Kim, Sanghun Kim, Kiu-Hyung Cho, Jin-Hyung Lee, Jintae Lee
Summary: Bacterial biofilm formation can be effectively inhibited by cinnamaldehyde and its derivatives, especially 4-nitrocinnamaldehyde. These compounds exhibit strong antibiofilm activities against UPEC and S. aureus, making them promising candidates for the treatment of biofilm-associated diseases.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Chemistry, Multidisciplinary
Cai-Qin Wu, An-An Liu, Xu Li, Jia-Wei Tu, Juan Kong, Ling-Ling Yang, Jian-Hong Jia, Chuan Wang, Bin Hu, Zhi-Xiong Xie, Dai-Wen Pang
Summary: The intracellular redox environment plays a critical role in regulating the fluorescence properties of biosynthesized CdSe QDs in Staphylococcus aureus.
SCIENCE CHINA-CHEMISTRY
(2023)
Article
Chemistry, Physical
Hong Kang, Yanyun Ju, Ting Han, Sixia Ye, Guanghui Zhao, Lijie Dong
Summary: A new strategy for effective and rapid detection of fingerprints based on electrospun nanofibrous membranes and quantum dots (QDs) is proposed in this study. The results show that fingerprints can be recorded within 30 seconds using QDs and the obtained fingerprints are invisible in sunlight.
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
(2021)
Article
Spectroscopy
Weikang Yang, Xinyu Zheng, Feng Gao, Huihui Li, Bo Fu, Dong-Yu Guo, Fuxiang Wang, Qinhe Pan
Summary: A CdTe QDs@ZIF-8 composite was prepared and applied for the detection of CTC residue in animal food products. The composite exhibited a short response time and a low detection limit, and showed excellent recyclability. Visual determination of CTC was achieved through the manufacture of portable test strips.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2022)
Article
Chemistry, Analytical
Qian Wu, Li Tian, Xiangyu Shan, Huiling Li, Chao Li, Juan Lu
Summary: A novel ECL sensor was developed by modifying Fe3CuO4-Ru(bpy)3+ and GO-CdS@ZnS on the electrode for the detection of permethrin. The sensor exhibited high sensitivity and a wide detection range.
Article
Chemistry, Analytical
Tingting Gong, Lei Liao, Bingying Jiang, Ruo Yuan, Yun Xiang
Summary: In this study, a fluorescent biosensing system for high sensitivity detection of MT was established using a new Ag+-stabilized DNA triplex probe (Ag+-SDTP) combined with catalytic hairpin assembly (CHA) and CRISPR/Cas12a signal enhancements. The sensing method is able to selectively detect MT from other non-specific molecules and can achieve low level detection of MT in diluted human serums, demonstrating its potential for monitoring disease-specific MT biomarker at trace levels.
Article
Chemistry, Analytical
Estrella Sanz Rodriguez, Kyra Wood, Paul R. Haddad, Brett Paull
Summary: This study presents a simple, rapid, and reliable method for determining boron in aqueous solutions using suppressed ion chromatography coupled to electrospray ionisation-triple quadrupole mass spectrometry. The method achieved a low limit of detection and demonstrated excellent analytical performance.
Article
Chemistry, Analytical
Aline Cournut, Paul Moustiez, Yannick Coffinier, Christine Enjalbal, Claudia Bich
Summary: Alzheimer's disease is a major cause of senile dementia and is characterized by the aggregation of neurofibrillary tangles and amyloid plaques in the brain. The A beta 1-42 peptide is considered the primary cause of neurotoxicity due to its insolubility and subsequent aggregation. A beta 1-42 and A beta 1-40 have been identified as relevant biomarkers for AD diagnosis, and SurfaceAssisted Laser Desorption/Ionization Mass Spectrometry (SALDI-MS) was used to monitor the A beta 1-42/A beta 1-40 ratio without prior sample treatment.
Article
Chemistry, Analytical
Dotse Selali Chormey, Buse Tugba Zaman, Tulay Borahan Kustanto, Sezin Erarpat Bodur, Suleyman Bodur, Elif Ozturk Er, Sezgin Bakirdere
Summary: This review provides an overview of the harmful effects of endocrine disrupting chemicals (EDCs) on human health and introduces the methods for analyzing EDCs. Recent research has focused on using green chemicals, such as deep eutectic solvents (DESs), for microextraction to ensure environmental safety.
Article
Chemistry, Analytical
Yan Zhang, Renfeng Xu, Jingjing Wu, Zhenghong Zhang, Yuhuang Wang, Hongqin Yang, Sheng Zhang
Summary: This study visualized the pore-like structures in prostate cancer cell membranes after treatment with the anticancer drug paclitaxel, demonstrating its mechanism of action related to oxidative damage.
Article
Chemistry, Analytical
Chihong Ma, Qin Zhou, Jinjin Shi, Hua Gao, Di Huang, Huimin Xue, Han Wang, Zhenzhong Zhang, Sen Yang, Junli Zhang, Kaixiang Zhang
Summary: We developed a CRISPR-empowered electrochemical biosensor, named PER-E-CRISPR, for amplification-free and sensitive detection of miR-21. The biosensor combines the advantages of CRISPR/Cas13a and primer exchange reaction (PER) for target amplification. Under optimized conditions, the PER-E-CRISPR assay exhibits a linear range from 10-13 to 10-7 M and a limit of detection of 30.2 fM. The biosensor shows excellent performance in actual plasma, indicating promising prospects for miRNA detection in molecular diagnosis.
Article
Chemistry, Analytical
Gang Liang, Jie Zhao, Yufei Gao, Tao Xie, Jianhui Zhen, Ligang Pan, Wenwen Gong
Summary: Molecular docking (MD) analysis is widely used for studying the interaction between aptamers and small molecules. This study established steady-state aptamers of tertiary structures (SATS) using specific aptamers of tetracycline antibiotics as docking models. Molecular docking results revealed multiple binding sites in the SATS of aptamers, with significant variations in binding free energy (BFE) and docking score (DS). The study also proposed a method for MD analysis based on SATS, providing insights into binding mode and predicting binding sites.
Article
Chemistry, Analytical
Yixuan Xu, Changchang Teng, Huiping Dang, Dalong Yin, Lifeng Yan
Summary: Stable organic radical molecules with high NIR fluorescence quantum efficiency have been synthesized and encapsulated into nanoparticles, which show great potential for both photodynamic therapy (PDT) and photothermal therapy (PTT) of tumors.
Article
Chemistry, Analytical
Yu Su, Dandan Yang, Yanjie Wang, Jie Ding, Lan Ding, Daqian Song
Summary: In this study, a highly selective molecularly imprinted polymer Cu-MIPs@CS was designed and synthesized for bisphenol A (BPA). Cu-MIPs@CS showed significantly enhanced imprinting factor and selective factor towards BPA compared to MIPs@CS. It also exhibited superior discrimination ability between BPA and its structural analogue. Additionally, Cu-MIPs@CS were successfully applied as a solid phase extraction adsorbent for the determination of BPA in drinking water samples.
Article
Chemistry, Analytical
Yi Qiao, Qiongdan Zhang, Yukun He, Tianguang Cheng, Jing Tu
Summary: Single cell heterogeneity plays an important role in many biological phenomena, and distinguishing cells with specific mutations is beneficial for clinical diagnosis and drug screening. This study introduces a gel plate platform that can distinguish cell types based on their phenotypes, with low equipment requirement. The gel platform has potential in point-of-care circumstances and single-cell stimulation responses that prioritize efficiency and simplicity.
Article
Chemistry, Analytical
Chengke Wang, Shuyang Sun, Ping Wang, Huawei Zhao, Wenling Li
Summary: The food safety of aquatic products is a global concern. Nanotechnology-based analyses have advantages in the detection of bacteria, metal ions, and small molecule contaminants. This review summarizes the recent advances in biosensing strategies for aquatic products and highlights the application of nanomaterials, lateral flow-based biosensors, surface-enhanced Raman scattering, microfluidic chips, and molecular imprinting technologies.
Article
Chemistry, Analytical
Liping Zhu, Zeng Tang, Xuemei Zhang, Li Zhu, Tian Meng, Linying Yu, Ting Xiao, Shasha Lu, Xiaoli Xiong, Xiurong Yang
Summary: A sensitive off-on electrochemiluminescence DNA sensor was developed using Exo III-assisted cascade amplification system. The sensor achieved high selectivity and ultra-high sensitivity in detecting target DNA by utilizing ECL and SPR effects. This study provides a new perspective for designing highly sensitive and programmable ECL biosensors.
Article
Chemistry, Analytical
Tong Li, Mengxu Sun, Suping Xia, Ting Huang, Rong-Tian Li, Chunrong Li, Zong Dai, Jin-Xiang Chen, Jun Chen, Nuan Jia
Summary: Here, we developed a binary system based on DNA tetrahedron and fluorogenic RNA aptamers for highly specific and label-free mRNA imaging in living cells. This system achieved high specificity label-free detection of nucleic acids with a detection limit of 1.34 nM. The method has potential applications in early disease diagnosis and new drug development.
Article
Chemistry, Analytical
Yao Zhao, Shu Zhang, Wang Yao, Yuxuan Zhu, Jing Qian, Juan Yang, Nianjun Yang
Summary: A new hierarchical nanostructured composite with high conductivity and catalytic activity is synthesized. The sensor exhibits significant catalytic effect and high sensitivity for monitoring the environmental endocrine disruptor bisphenol A (BPA).