期刊
HORTICULTURE ENVIRONMENT AND BIOTECHNOLOGY
卷 56, 期 1, 页码 94-104出版社
KOREAN SOC HORTICULTURAL SCIENCE
DOI: 10.1007/s13580-015-0084-3
关键词
Agroboeterium tumefaciens; genetic transformation; proline content; salinity stress; transgene inheritance
类别
资金
- National Key Project of GMO Crops [2009ZX08004-011B]
- Priority Academic Program Development of Jiangsu Higher Education Institutions [2011PAPD]
- Specialized Research Fund for the Doctoral Program of Higher Education of China [20110097120009]
Vegetable soybeans [Glycine may (L.) Merrill] are susceptible to salt stress and, thus, soil salinity can severely affect their growth and productivity. To enhance the salt tolerance of vegetable soybeans, a novel Solanum torvum Swartz Delta(1)-pyrroline-5-caroxylate synthetase gene (StP5CS, GenBank accession number: JN606861) that encodes a critical regulatory enzyme in proline biosynthesis was transformed into the cultivar NY-1001 using an Agrobacterium-mediated transformation method. PCR and Southern blot analyses indicated that two independent To fertile transgenic plants were generated. The transgenic plants transmitted the transgenes into their T-1 progeny in a 3:1 ratio. Me T-2 and T-3 homozygous transgenic lines (IITLs) were examined for salt tolerance in pot and hydroponic cultures, respectively. The StP5CS overexpression conferred salt tolerance in T-2 and T-3 HTLs. Under NaCl stress conditions, the leaf scorch scores of T2 and T3 HTLs were significantly lower than those of wild-type (W) plants. The plant height, leaf area, relative chlorophyll content, and number of fresh pods of 12 and T3 PITLs were significantly higher than those of WT plants. Compared with WE plants, T-2 and T-3 IHTts had significantly higher levels of proline and significantly lower levels of membrane lipid peroxidation. These results indicate that StP5CS expression in HTLs results in enhanced salt tolerance associated with higher levels of proline accumulation under salinity stress and that StP5CS can be utilized to improve salinity tolerance in vegetable crop genetic engineering.
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