Article
Biochemistry & Molecular Biology
Jia Xuan Leong, Margot Raffeiner, Daniela Spinti, Gautier Langin, Mirita Franz-Wachtel, Andrew R. Guzman, Jung-Gun Kim, Pooja Pandey, Alyona E. Minina, Boris Macek, Anders Hafren, Tolga O. Bozkurt, Mary Beth Mudgett, Frederik Boernke, Daniel Hofius, Suayib Uestuen
Summary: Autophagy plays a crucial role in plant-bacteria interactions, where antimicrobial autophagy degrades intracellular pathogens and reveals a pathogen strategy of escaping elimination by hijacking the autophagy mechanism.
Article
Multidisciplinary Sciences
Tao-Tao Chen, Yanling Lin, Shijun Zhang, Shuxin Liu, Lei Song, Wenhong Zhong, Zhao-Qing Luo, Aidong Han
Summary: The intracellular bacterial pathogen Legionella pneumophila hijacks host vesicular transport factor p115 to localize its effector protein LegA15 in host lipid droplets (LDs) and causes fragmentation of host Golgi apparatus. LegA15 is an atypical GTPase that modulates host LD homeostasis through its GTPase activity.
Review
Immunology
Guy Tran Van Nhieu, Patricia Latour-Lambert, Jost Enninga
Summary: Shigella's type III effector IpgD plays a key role in disrupting the cortical actin filaments of epithelial cells by decreasing PI4,5P(2) levels, facilitating bacterial invasion and cell migration. Additionally, IpgD also stimulates various signaling pathways and regulates endosomal trafficking and immune responses, making it a versatile bacterial enzyme.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Sun Cheol Park, So Yeon Cho, Tae Hee Kim, Kyung Yeol Ko, Wan Seok Song, Seung Goo Kang, Geun-Shik Lee, Sung-il Yoon
Summary: LegK7 is an atypical kinase that activates through interaction with the host MOB1 protein without the need for phosphorylation. Upon binding with MOB1, LegK7 undergoes a structural reshaping that allows it to adopt a closed conformation compatible with ATP binding.
JOURNAL OF MOLECULAR BIOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Sahel Amoozadeh, Jodie Johnston, Claudia-Nicole Meisrimler
Summary: Interactions between pathogenic fungi and oomycetes with plants can have different impacts on plant health, with effector proteins playing a key role in successful colonization of the host plant. Investigating the biological and functional roles of effectors through bioinformatics and experimental approaches is crucial for understanding plant-microbe interactions. The use of next generation protein modeling software like RoseTTafold and AlphaFold2, which utilize novel machine-learning algorithms, has advanced the field of effector biology and made these methods more accessible to users.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Multidisciplinary Sciences
Zhiqiang Hu, Yingchao Xie, Jiansen Lu, Jianwu Yang, Jiahuan Zhang, Huaji Jiang, Hongyu Li, Yufeng Zhang, Dan Wu, Ke Zeng, Xiaochun Bai, Xiao Yu
Summary: In this study, VANGL2 was found to be an IFN-inducible negative feedback regulator that suppresses IFN-I signaling during VSV infection. VANGL2 interacts with TBK1 and promotes the autophagic degradation of TBK1 via K48-linked polyubiquitination mediated by TRIP. Myeloid-specific deletion of VANGL2 in mice results in enhanced IFN-I production against VSV infection and improved survival. These findings reveal a negative feedback loop of IFN-I signaling through the VANGL2-TRIP-TBK1-OPTN axis and highlight the importance of the crosstalk between IFN-I and autophagy in preventing viral infection. VANGL2 could be a potential clinical therapeutic target for viral infectious diseases, including COVID-19.
Article
Biology
Kangcheng Song, Miao Wei, Wenjun Guo, Li Quan, Yunlu Kang, Jing-Xiang Wu, Lei Chen
Summary: TRPC5 channel is a nonselective cation channel involved in various physiological processes. Inhibitors of TRPC5, such as clemizole and HC-070, stabilize the ion channel in a nonconductive closed state, potentially offering new therapeutic strategies for anxiety disorders, depression, and kidney diseases. The cryo-EM structures of human TRPC5 with inhibitors provide insights into the binding sites and inhibitory mechanisms, facilitating the development of more effective inhibitors targeting TRPC5.
Article
Biology
Pooja Pandey, Alexandre Y. Leary, Yasin Tumtas, Zachary Savage, Bayantes Dagvadorj, Cian Duggan, Enoch L. H. Yuen, Nattapong Sanguankiattichai, Emily Tan, Virendrasinh Khandare, Amber J. Connerton, Temur Yunusov, Mathias Madalinski, Federico Gabriel Mirkin, Sebastian Schornack, Yasin Dagdas, Sophien Kamoun, Tolga O. Bozkurt
Summary: The effector protein PexRD54 secreted by the Irish famine pathogen Phytophthora infestans can induce autophagosome formation and promote the generation of autophagosomes by utilizing lipid droplets through specific trafficking pathways. This effector protein mimics starvation-induced autophagy to alter endomembrane trafficking at the host-pathogen interface, facilitating colonization by the pathogen.
Review
Immunology
Priya Bhatnagar, Gopinathan Pillai Sreekanth, Kaja Murali-Krishna, Anmol Chandele, Ramakrishnan Sitaraman
Summary: This review article discusses the role of Dengue virus non-structural protein 5 (NS5) and its interactions with other proteins in the human host, particularly focusing on its interference with anti-viral interferon signaling. By annotating and consolidating known and potential NS5 interactors using experimental and computational approaches, the study provides insights for further research efforts to understand how NS5 manipulates the human-virus interface and immune responses.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Kuo Zhang, Ting Peng, Xinyuan Tao, Miao Tian, Yanxin Li, Zhao Wang, Shuaifei Ma, Shufan Hu, Xing Pan, Juan Xue, Jiwei Luo, Qiulan Wu, Yang Fu, Shan Li
Summary: This study reveals the multistep enzymatic mechanism of CopC-catalyzed arginine ADPR deacylization in resisting bacterial infections, providing a structural framework for understanding the molecular basis of this process.
Article
Multidisciplinary Sciences
Maria Teresa Bueno-Carrasco, Jorge Cuellar, Marte I. Flydal, Cesar Santiago, Trond-Andre Krakenes, Rune Kleppe, Jose R. Lopez-Blanco, Miguel Marcilla, Knut Teigen, Sara Alvira, Pablo Chacon, Aurora Martinez, Jose M. Valpuesta
Summary: Tyrosine hydroxylase (TH) is a key enzyme in the synthesis of dopamine (DA) and other catecholamines. This study used Cryo-EM to determine the structures of human TH with and without DA, as well as S40 phosphorylated TH. The study revealed the inhibitory and stabilizing effects of DA, as well as the counteraction by S40 phosphorylation, providing insights into the regulatory mechanisms of TH and dopamine homeostasis.
NATURE COMMUNICATIONS
(2022)
Article
Multidisciplinary Sciences
Gabriel U. Oka, Diorge P. Souza, William Cenens, Bruno Y. Matsuyama, Marcus V. C. Cardoso, Luciana C. Oliveira, Filipe da Silva Lima, Iolanda M. Cuccovia, Cristiane R. Guzzo, Roberto K. Salinas, Chuck S. Farah
Summary: The study identified an interaction between XVIPCD and VirD4, dividing XVIPCD into two regions with distinct functions. The N-terminal region interacts with VirD4, while both the N- and C-terminal regions are required for efficient X-Tfe translocation into target cells. The conformational stability of the N-terminal region is reduced at lower pH, potentially facilitating X-Tfe unfolding and translocation.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Biochemistry & Molecular Biology
Dong Man Jang, Hyo Jin Lim, Hyunggu Hahn, Yeon Lee, Hark Kyun Kim, Hyoun Sook Kim
Summary: By repurposing a Janus kinase 1 inhibitor, ruxolitinib, as a DCLK1 inhibitor, researchers were able to show micromolar binding affinity and inhibitory activity. The crystal structure of ruxolitinib-complexed DCLK1 provides insight into the interaction mode and could potentially guide the discovery of selective DCLK1 inhibitors.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Multidisciplinary Sciences
Kirill D. Nadezhdin, Leonor Correia, Chamali Narangoda, Dhilon S. Patel, Arthur Neuberger, Thomas Gudermann, Maria G. Kurnikova, Vladimir Chubanov, Alexander I. Sobolevsky
Summary: The transient receptor potential channel TRPM7 is a crucial regulator of divalent cation balance in the body, impacting various biological processes such as embryonic development, immune responses, and cell functionalities. It is linked to neuronal and cardiovascular disorders as well as tumor progression, highlighting its potential as a drug target. Using cryo-EM, functional analysis, and molecular dynamics simulations, researchers have unraveled two distinct structural mechanisms behind TRPM7 activation through gain-of-function mutations and the agonist naltriben. These mechanisms exhibit different conformational dynamics and involve different domains. Additionally, the study identifies a binding site for potent and selective inhibitors that stabilize the closed state of TRPM7, paving the way for the development of drugs targeting TRPM7-related channelopathies.
NATURE COMMUNICATIONS
(2023)
Article
Multidisciplinary Sciences
Simona Pilotto, Thomas Fouqueau, Natalya Lukoyanova, Carol Sheppard, Soizick Lucas-Staat, Luis Miguel Diaz-Santin, Dorota Matelska, David Prangishvili, Alan C. M. Cheung, Finn Werner
Summary: The study investigates two complexes that inhibit RNA polymerase, from Acidianus two-tailed virus and Sulfolobus Turreted Icosahedral Virus, revealing their mechanisms of transcription inhibition. The findings are important for understanding the inhibitory effects of archaeal eukaryotic-like RNA polymerases in virus infection.
NATURE COMMUNICATIONS
(2021)
Article
Multidisciplinary Sciences
Laurie Pinaud, Mariana L. Ferrari, Robin Friedman, Nico Jehmlich, Martin von Bergen, Armelle Phalipon, Philippe J. Sansonetti, Francois-Xavier Campbell-Valois
Editorial Material
Biochemistry & Molecular Biology
Karen M. Dunkerley, Gary S. Shaw
Article
Biochemistry & Molecular Biology
Atul Kumar, Viduth K. Chaugule, Tara E. C. Condos, Kathryn R. Barber, Clare Johnson, Rachel Toth, Ramasubramanian Sundaramoorthy, Axel Knebel, Gary S. Shaw, Helen Walden
NATURE STRUCTURAL & MOLECULAR BIOLOGY
(2017)
Review
Biochemistry & Molecular Biology
Eugene Kang, Alanna Crouse, Lucie Chevallier, Stephanie M. Pontier, Ashwag Alzahrani, Navoun Silue, Francois-Xavier Campbell-Valois, Xavier Montagutelli, Samantha Gruenheid, Danielle Malo
Article
Biochemistry & Molecular Biology
Tara E. C. Condos, Karen M. Dunkerley, E. Aisha Freeman, Kathryn R. Barber, Jacob D. Aguirre, Viduth K. Chaugule, Yiming Xiao, Lars Konermann, Helen Walden, Gary S. Shaw
Article
Biochemistry & Molecular Biology
Donald E. Spratt, Kathryn R. Barbers, Nicole M. Marlatt, Vy Ngo, Jillian A. Macklin, Yiming Xiao, Lars Konermann, Martin L. Duennwald, Gary S. Shaw
Article
Biochemical Research Methods
Navoun Silue, Endrei Marcantonio, F-X Campbell-Valois
Article
Cell Biology
Truc T. Losier, Mercy Akuma, Olivia C. McKee-Muir, Nicholas D. LeBlond, Yujin Suk, Reham M. Alsaadi, Zhihao Guo, Ryan Reshke, Subash Sad, Francois-Xavier Campbell-Valois, Derrick J. Gibbings, Morgan D. Fullerton, Ryan C. Russell
Article
Microbiology
Jean-Yves Tinevez, Ellen T. Arena, Mark Anderson, Giulia Nigro, Louise Injarabian, Antonin Andre, Mariana Ferrari, Francois-Xavier Campbell-Valois, Anne Devin, Spencer L. Shorte, Philippe J. Sansonetti, Benoit S. Marteyn
NATURE MICROBIOLOGY
(2019)
Article
Immunology
Giulia Nigro, Ellen T. Arena, Martin Sachse, Maryse Moya-Nilges, Benoit S. Marteyn, Philippe J. Sansonetti, F. -X. Campbell-Valois
PATHOGENS AND DISEASE
(2019)
Review
Microbiology
Waad Bajunaid, Nathaline Haidar-Ahmad, Anwer Hasil Kottarampatel, France Ourida Manigat, Navoun Silue, Caetanie F. Tchagang, Kyle Tomaro, Francois-Xavier Campbell-Valois
Article
Microbiology
Navoun Silue, Francois-Xavier Campbell-Valois
Summary: Shigella utilizes a Type III Secretion Apparatus to translocate proteins into host cells, including two newly discovered chromosomal genes - icaR and icaT, which can also be activated by MxiE and IpgC. These genes are secreted by T3SA independently of chaperones, and have orthologs in various E. coli strains belonging mainly to phylogroups A, B1, D, and E.
Review
Biochemistry & Molecular Biology
ZiWei Li, Gary S. Shaw
Summary: Cell membrane repair is a crucial process for cell survival and integrity, which involves multiple mechanisms such as exocytosis, endocytosis, patching, and shedding. Calcium-sensor proteins, including synaptotagmins, dysferlin, S100 proteins, and annexins, play a regulatory or participatory role in membrane repair. Dysfunctions or genetic alterations in these proteins can lead to diseases such as muscular dystrophy and heart disease. This review focuses on the role of key calcium-sensor proteins in membrane repair.
BIOSCIENCE REPORTS
(2023)
Article
Microbiology
Jielu Yan, Bob Zhang, Mingliang Zhou, Francois-Xavier Campbell-Valois, Shirley W. I. Siu
Summary: Antimicrobial peptides (AMPs) are potential candidates for replacing conventional antibiotics to combat drug resistance in pathogenic bacteria. To accelerate the evaluation process, we develop a deep learning method called MBC-Attention to predict the minimum inhibitory concentration of AMPs against Escherichia coli. The proposed model outperforms traditional machine learning methods.
Review
Microbiology
Nathaline Haidar-Ahmad, France Ourida Manigat, Navoun Silue, Stephanie M. Pontier, Francois-Xavier Campbell-Valois
Summary: Shigella spp. cause a large number of intestinal infections each year, targeting the mucosa of the human colon. They are an important model for studying intracellular bacterial pathogenesis. Shigella is a pathovar of Escherichia coli and is characterized by the presence of a plasmid, pINV, which encodes the type III secretion system and icsA for cytosol invasion and cell-to-cell spread, respectively. Recent research has focused on the genetic aspects, maintenance of pINV, and the role of transcription activators in virulence gene regulation. The interplay between pINV-encoded activators and chromosome-encoded factors is also explored, along with novel chromosomal genes regulated by MxiE.