Article
Biotechnology & Applied Microbiology
Sara Lupacchini, Jens Appel, Ron Stauder, Paul Bolay, Stephan Klaehn, Elisabeth Lettau, Lorenz Adrian, Lars Lauterbach, Bruno Buehler, Andreas Schmid, Joerg Toepel
Summary: This study successfully addressed the challenge of oxygen release during photosynthesis-driven H-2 production by introducing an oxygen-tolerant hydrogenase into cyanobacteria. The hydrogenase enabled the bacteria to utilize H-2 to fix CO2 and produce H-2 under different conditions, laying a foundation for further engineering advancements.
METABOLIC ENGINEERING
(2021)
Article
Biology
Leonardo Vinicius Monteiro de Assis, Lisbeth Harder, Jose Thalles Lacerda, Rex Parsons, Meike Kaehler, Ingolf Cascorbi, Inga Nagel, Oliver Rawashdeh, Jens Mittag, Henrik Oster, Matthew A. Quinn
Summary: This study demonstrates that fluctuations in thyroid hormone (TH) levels restructuring the diurnal liver metabolic transcriptome independent of local molecular circadian clocks, which affects energy metabolism processes in the liver.
Article
Gastroenterology & Hepatology
Tuo Hu, Surendra K. Shukla, Enza Vernucci, Chunbo He, Dezhen Wang, Ryan J. King, Kanupriya Jha, Kasturi Siddhanta, Nicholas J. Mullen, Kuldeep S. Attri, Divya Murthy, Nina Chaika, Ravi Thakur, Scott E. Mulder, Camila G. Pacheco, Xiao Fu, Robin R. High, Fang Yu, Audrey Lazenby, Clemens Steegborn, Ping Lan, Kamiya Mehla, Dante Rotili, Sarika Chaudhary, Sergio Valente, Marco Tafani, Antonello Mai, Johan Auwerx, Eric Verdin, David Tuveson, Pankaj K. Singh
Summary: SIRT5 functions as a key tumor suppressor in PDAC, inhibiting tumor cell proliferation and correlating with a favorable prognosis. Loss of SIRT5 promotes PDAC development and malignant transformation.
Article
Multidisciplinary Sciences
Zeenat Rashida, Rajalakshmi Srinivasan, Meghana Cyanam, Sunil Laxman
Summary: In changing environments, cells modulate resource budgeting through distinct metabolic routes to control growth. The TORC1 and SNF1/AMPK pathways operate contrastingly in nutrient replete or limited environments to maintain homeostasis. Kog1, a modified form of yeast TORC1 component, plays a crucial role in regulating SNF1/AMPK activity and outputs during glucose and amino acid limitations, facilitating effective carbon allocation and metabolic flux.
Review
Immunology
Ningyue Sun, Yangzi Tian, Yuhan Chen, Weinan Guo, Chunying Li
Summary: Melanoma is a lethal type of skin cancer that is characterized by disordered metabolism. Metabolic rewiring in melanoma not only affects tumor cell behaviors but also interferes with antitumor immunity by influencing the functions of surrounding immune cells. This review summarizes the epidemiology, clinical characteristics, and treatment proceedings of melanoma, as well as the roles of immune cells and metabolic rewiring in tumor immunology.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Gabriela L. Oliveira, Ana R. Coelho, Ricardo Marques, Paulo J. Oliveira
Summary: This review summarizes how cells adapt their metabolism to survive in tumoral environment conditions and the crucial role of mitochondria in tumor metabolic programing and therapeutics. Mitochondria play a key role in carcinogenesis and tumor progression and could be potential therapeutic targets.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE
(2021)
Article
Biochemistry & Molecular Biology
Maeve Long, Alvaro Sanchez-Martinez, Marianna Longo, Fumi Suomi, Hans Stenlund, Annika Johansson, Homa Ehsan, Veijo T. Salo, Lambert Montava-Garriga, Seyedehshima Naddafi, Elina Ikonen, Ian G. Ganley, Alexander J. Whitworth, Thomas G. McWilliams
Summary: Mitophagy removes defective mitochondria through lysosomal elimination. Iron depletion triggers lipid metabolism remodeling and lipid droplets bordering mitochondria. Inhibiting DGAT1 restricts mitophagy and leads to cell death.
Article
Mathematics, Applied
Jiaxin Zhou, Hongli Wang, Qi Ouyang
Summary: This paper proposes a temporal SCN network model and investigates the effects of dynamical rewiring and flexible coupling due to synaptic plasticity on the synchronization of the neural network in SCN. The results suggest that both dynamical rewiring and coupling plasticity enhance the synchronization in inhomogeneous networks, and a proper network model for the master SCN circadian rhythm needs to take into account the effects of dynamical changes in topology and plasticity in neuron interactions.
Article
Cell Biology
Inna Rabinovich-Nikitin, Mina Rasouli, Cristine J. Reitz, Illana Posen, Victoria Margulets, Rimpy Dhingra, Tarak N. Khatua, James A. Thliveris, Tami A. Martino, Lorrie A. Kirshenbaum
Summary: This study reveals that the CLOCK gene transcriptionally coordinates mitochondrial quality control mechanisms in cardiac myocytes, regulating an adaptive stress response crucial for cell survival. Restoring CLOCK activity may be beneficial in reducing cardiac injury in individuals with disrupted circadian rhythms.
Review
Biochemistry & Molecular Biology
Jong-Whi Park
Summary: Multiple metabolic pathways play important roles in maintaining cellular homeostasis. Altered cell metabolism has been shown to contribute significantly to the biology of gliomas, prompting current research efforts to better understand the metabolic rewiring between glioma genotype and tissue context. Molecular profiling has identified activated oncogenes and inactivated tumor suppressors that impact cellular metabolism and are associated with glioma pathogenesis. Mutations in isocitrate dehydrogenases (IDHs) have emerged as crucial prognostic factors in adult diffuse gliomas. This review provides an overview of metabolic alterations in IDH-mutant gliomas and IDH-wildtype glioblastoma (GBM), with a focus on targeting metabolic vulnerabilities for the development of new therapeutic strategies.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Editorial Material
Medicine, Research & Experimental
Jonathan Cedernaes, Joseph Bass
Summary: The study found that REV-ERB alpha and -beta in the tuberal hypothalamus are essential for maintaining leptin responsiveness and metabolic homeostasis, providing insight into how transcriptional changes may link energy-sensing cell types with day/night rhythms.
JOURNAL OF CLINICAL INVESTIGATION
(2021)
Review
Biochemistry & Molecular Biology
Filip Vujovic, Claire E. Shepherd, Paul K. Witting, Neil Hunter, Ramin M. Farahani
Summary: This article reviews the network topology of key signaling pathways, with a focus on redox-sensitive proteins such as PTEN and Ras GTPase, which have the ability to reshape the connectivity profile of signaling pathways based on altered redox states. It proposes that redox-mediated rewiring is essential for regulating the rate of transmission of biological signals and suggests the existence of a programmable cellular clock that orchestrates the pace of biological phenomena. Furthermore, it discusses the evidence that abnormal redox-mediated modulation of the cellular clock output contributes to pathological conditions affecting the human brain.
Article
Biochemistry & Molecular Biology
Ali Ghanem, Anna Maria Melzer, Esther Zaal, Laura Neises, Danny Baltissen, Omar Matar, Hannah Glennemeier-Marke, Fadi Almouhanna, Jannick Theobald, Mohamed A. Abu el Maaty, Celia Berkers, Stefan Woelfl
Summary: The use of high doses of ascorbate for cancer treatment has shown efficacy, particularly in inducing cytotoxicity against breast cancer cells through metabolic rewiring and disruption of cellular homeostasis. The mechanism of action involves oxidative stress, ROS accumulation, DNA damage, and altered metabolic pathways, leading to irreverisble cell death.
FREE RADICAL BIOLOGY AND MEDICINE
(2021)
Article
Multidisciplinary Sciences
Shanshan Zhang, Jiahui Sun, Dandan Feng, Huili Sun, Jinyu Cui, Xuexia Zeng, Yannan Wu, Guodong Luan, Xuefeng Lu
Summary: This study demonstrates that inhibiting native glucokinase activity can enhance photosynthetic glucose production in Synechococcus elongatus PCC 7942. The glucose secretion reaches 5g/L through metabolic and cultivation engineering. This research highlights the metabolic plasticity of cyanobacteria and their potential for direct photosynthetic glucose production.
NATURE COMMUNICATIONS
(2023)
Article
Multidisciplinary Sciences
Meng Qu, Guoxin Zhang, Han Qu, Alexander Vu, Raymond Wu, Hidekazu Tsukamoto, Zhenyu Jia, Wendong Huang, Heinz-Josef Lenz, Jeremy N. Rich, Steve A. Kay
Summary: Hepatocellular carcinoma (HCC) is a global health challenge with increasing incidence worldwide. The circadian clock has been shown to play a key role in hepatocarcinogenesis, but the cellular and molecular mechanisms underlying the HCC-clock crosstalk are unknown. In this study, we found that HCC cells rely on the circadian clock transcription factors BMAL1 and CLOCK for cell growth, and down-regulation of these factors induces apoptosis and cell cycle arrest. Mechanistically, inhibiting BMAL1/CLOCK dysregulates cell cycle regulators Wee1 and p21, leading to tumor cell death. Our findings provide insights into the cellular impact of clock proteins in HCC oncogenesis and suggest a potential therapeutic approach based on modulation of the circadian clock.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)