Article
Environmental Sciences
Yu-Syuan Luo, Su-Yin Chiang, Tai-Ying Long, Tung-Hu Tsai, Kuen-Yuh Wu
Summary: The study utilized a novel MD-LC-MS/MS method to investigate the TKs of AA and its metabolites, demonstrating the metabolic advantage of AA being oxidized to GA. This method provides excellent temporal resolution for capturing metabolic saturation and is a promising approach for studying toxicokinetics.
ENVIRONMENT INTERNATIONAL
(2022)
Article
Medical Laboratory Technology
Daniel Zhi Wei Ng, Sean Xian Yu Lee, Delicia Shu Qin Ooi, Le Duc Huy Ta, Gaik Chin Yap, Carina Jing Xuan Tay, Chiung-Hui Huang, Elizabeth Huiwen Tham, Evelyn Xiu Ling Loo, Lynette P. C. Shek, Anne Goh, Hugo P. S. Van Bever, Oon Hoe Teoh, Yung Seng Lee, Fabian Yap, Kok Hian Tan, Yap-Seng Chong, Shiao Yng Chan, Johan Gunnar Eriksson, Keith M. Godfrey, Bee Wah Lee, Eric Chun Yong Chan
Summary: This study developed a liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for the high-throughput analysis of gut microbial and dietary-derived metabolites in human plasma. The method is sensitive and can quantify these metabolites within 9 minutes without prior sample derivatization, providing technical support for studying the role of these metabolites in maternal inflammation.
CLINICA CHIMICA ACTA
(2023)
Article
Environmental Sciences
Aaron J. Pandopulos, Richard Bade, Benjamin J. Tscharke, Jake W. O'Brien, Bradley S. Simpson, Jason M. White, Cobus Gerber
Summary: This study evaluated catecholamine metabolites as potential population biomarkers and found high correlations and similarities in some wastewater catchments in Australia, indicating their suitability as markers for future studies.
SCIENCE OF THE TOTAL ENVIRONMENT
(2021)
Article
Chemistry, Analytical
Yi Dai, Chu-Bo Qi, Yang Feng, Qing-Yun Cheng, Fei-Long Liu, Ming-Yu Cheng, Bi-Feng Yuan, Yu-Qi Feng
Summary: The discovery of dynamic and reversible modifications in RNA has expanded their functional repertoires, offering new insight into biological processes. A novel chemical derivatization strategy combined with mass spectrometry analysis has been developed to sensitively and simultaneously determine uridine thiolation and hydroxylation modifications in eukaryotic RNA, enhancing detection sensitivity by up to 339-fold. This method has been used to identify a new modification of 5-hydroxyuridine (ho(5)U) in mammalian cell small RNAs, demonstrating superior capability in detecting low-abundance RNA modifications and potentially leading to the discovery of new modifications in RNA.
ANALYTICAL CHEMISTRY
(2021)
Review
Biochemistry & Molecular Biology
Yaroslav Shansky, Julia Bespyatykh
Summary: Bile acids are important in nutrient digestion and act as signaling molecules in the blood circulation. The gut microbiota plays a role in modifying the effects of bile acids. Depending on the composition of the microbiota, bile acids can support homeostasis or contribute to pathological conditions. Bile acids have potential as biomarkers for human pathology.
Article
Biochemistry & Molecular Biology
Dominika Olesova, Petra Majerova, Roman Hajek, Juraj Piest'ansky, Radana Brumarova, Alena Michalicova, Bernadeta Jurkanin, David Friedecky, Andrej Kovac
Summary: This study characterized the glycosphingolipid profile in rat brain tissue using hydrophilic interaction liquid chromatography coupled to high-resolution tandem mass spectrometry. It revealed the important roles of gangliosides and sulfatides in neurodevelopment, aging, and neurodegeneration. The semi-targeted screening identified changes in levels of sulfatides and GM1a gangliosides during the aging process, and elevated levels of GM3 gangliosides in the tauopathy rat model, indicating a higher rate of apoptotic processes.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Dmitry Senko, Anna Gorovaya, Elena Stekolshchikova, Nickolay Anikanov, Artur Fedianin, Maxim Baltin, Olga Efimova, Daria Petrova, Tatyana Baltina, Mikhail A. Lebedev, Philipp Khaitovich, Anna Tkachev
Summary: The study assessed blood lipidome alterations in a rat sciatic nerve crush injury model and found delayed systematic alterations in fatty acid metabolism after nerve injury.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Chemistry, Analytical
Yun Li, Dan Zhang, Yan Mo, Teng Zeng, Tongzhi Wu, Lingqiao Liu, Hua Zhang, Chang Chen
Summary: In this study, a LC-MS method was developed to simultaneously measure sex hormones and bile acids in rat plasma, which was validated and applicable for studying these compounds in rat plasma. This method may also be useful for other matrices and species.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2023)
Article
Biochemical Research Methods
Johannes B. Mueller-Reif, Fynn M. Hansen, Lisa Schweizer, Peter Treit, Philipp E. Geyer, Matthias Mann
Summary: Reversed-phase HPLC is commonly used in MS-based proteomics for peptide separation, with particle-packed capillary columns being predominantly used in nanoflow HPLC systems. Despite capillary columns being a standard for many years, they are expensive and have short lifetimes, leading many laboratories to produce their own in-house packed columns.
MOLECULAR & CELLULAR PROTEOMICS
(2021)
Article
Chemistry, Analytical
Ge-Ge Gong, Jie Zheng, Sha Li, Ya-Li Bai, Yu-Qi Feng
Summary: A LC-MS method was developed to simultaneously determine 5 RAs in human serum, with enhanced analysis throughput and increased detection sensitivity. The concentrations of three RAs in cancer patient serum samples were significantly decreased compared with health controls, indicating a potential role of RAs in cancer formation and development.
Article
Biochemistry & Molecular Biology
Myriam Mireault, Vivaldy Prinville, Leanne Ohlund, Lekha Sleno
Summary: Using a semi-targeted approach, the effect of acetaminophen on circulating bile acid profiles in rats was investigated. The presence of known bile acids, potential isomeric structures, glucuronide and sulfate conjugates was examined using a reverse-phase method coupled to a high-resolution quadrupole time-of-flight system. Statistically significant changes in bile acid profiles were found between different doses of acetaminophen, with certain compounds showing significant increase at the highest dose, potentially indicating drug-induced liver injury.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Microbiology
L. N. Lucas, K. Barrett, R. L. Kerby, Q. Zhang, L. E. Cattaneo, D. Stevenson, F. E. Rey, D. Amador-Noguez
Summary: Gut bacteria play a critical role in modifying primary bile acids synthesized by the host, leading to the production of secondary bile acids that can regulate metabolic processes and influence gut microbiota composition. Through screening 72 bacterial isolates, this study identified 43 isolates capable of modifying unconjugated bile acids, expanding the known diversity of bacteria involved in bile acid transformations. The discovery of novel microbial conjugated bile acids and previously undescribed species highlights the importance of understanding the interactions between the microbiome, bile acid pool composition, and human health.
Article
Agriculture, Dairy & Animal Science
Marko Rudar, Alexandra Gachman, Melissa Boersma
Summary: A reproducible and robust ultra-high performance liquid chromatography method was evaluated for the simultaneous determination of cysteine, cysteinylglycine, homocysteine, gamma-glutamylcysteine, and glutathione in solid tissue. The method will facilitate novel insights into tissue-specific and whole-body sulfur amino acid metabolism in livestock animals.
JOURNAL OF ANIMAL SCIENCE
(2023)
Article
Biochemical Research Methods
Isin T. Sakallioglu, Amith S. Maroli, Aline De Lima Leite, Robert Powers
Summary: This study introduces a rapid reversed-phase ultra-high-performance liquid chromatography-high resolution mass spectrometry method for mycobacterial lipidomics analysis, which achieves separation of various lipid classes specific to mycobacterium in a short runtime of 14 minutes. The method demonstrates high sensitivity and faster sample throughput compared to existing methods.
JOURNAL OF CHROMATOGRAPHY A
(2022)
Article
Chemistry, Analytical
Pasquale Linciano, Fabiana Russo, Cinzia Citti, Francesco Tolomeo, Roberta Paris, Flavia Fulvio, Nicola Pecchioni, Maria Angela Vandelli, Aldo Lagana, Anna Laura Capriotti, Giuseppe Biagini, Luigi Carbone, Giuseppe Gigli, Giuseppe Cannazza
Summary: The recent discovery of CBDP and Delta 9-THCP has raised questions about their presence and abundance in cannabis, leading to the synthesis of CBDPA and THCPA for quantitative analysis. A targeted analysis of CBD- and THC-type compounds in different cannabis samples was conducted, along with the identification of other heptyl homologs using ultrahigh performance liquid chromatography coupled to high-resolution mass spectrometry.
Article
Biochemistry & Molecular Biology
Olexander M. Semenenko, Victoria V. Lipson, Alina O. Sadchenko, Olga V. Vashchenko, Natalia A. Kasian, Liliia Sviechnikova, Longin M. Lisetski, Mykola L. Babak, Volodymyr M. Vakula, Oleksandr Borysov, Yuliia Holota, Sergey O. Zozulya, Petro O. Borysko, Olexander Mazepa
Summary: An efficient protocol for the synthesis of novel methotrexate-betulonic acid hybrids has been developed and their structures and properties have been fully established. The hybrids showed inferior interactions with artificial lipid membranes, possibly due to their increasing lipophilicity.
Article
Biochemistry & Molecular Biology
Mohadese Yaghoobi Anzabi, Piotr Cmoch, Roman Luboradzki, Zbigniew Pakulski
Summary: Friedelin and 3-acetoxyfriedel-3-en-2-one, known as friedelane triterpenoids, have been isolated from cork smoker wash solids and used as starting materials for the synthesis of new friedelane derivatives. Reduction reactions result in the formation of friedelinol and epi-cerin derivatives.
Article
Biochemistry & Molecular Biology
Detlef Thieme, Aniko Krumbholz, Martin Bidlingmaier, Christoph Geffert, Annika Hameder, Andreas Stoever, Matthias Graw, Annekathrin M. Keiler
Summary: Ethanol can affect testosterone metabolism and potentially influence steroid biosynthesis and blood steroid profiles. The study emphasizes the importance of sufficient time interval from food and ethanol intake during blood sampling, particularly for diagnostics in doping control.
Article
Biochemistry & Molecular Biology
Masanobu Murao, Tetsuo Imano, Yoshinobu Sato, Masaaki Nakajima
Article
Biochemistry & Molecular Biology
Jingyuan Wang, Ye Feng, Brian Liu, Wen Xie
Summary: Sulfation and desulfation of steroids are opposing processes that regulate steroid homeostasis and are involved in steroid-related diseases. Cytosolic sulfotransferase and steroid sulfatase modify and regulate steroids to affect hormonal dysregulation, metabolic diseases, and cancer.
Article
Biochemistry & Molecular Biology
Noah K. Babel, Brian J. Feldman
Summary: Our research identified a previously unrecognized ADAMTS1-PTN-Wnt pathway in regulating adipose precursor cell behavior. The non-muscle myosin protein MYH9 was found to be a key target of this pathway for modulating adipogenesis. These findings offer potential for developing novel therapeutics for obesity and related metabolic disorders.
Article
Biochemistry & Molecular Biology
Martiniano Bello
Summary: This study explores the structural and thermodynamic basis of molecular recognition between ERβ and DPN and its derivatives using molecular dynamics simulations and end-point methods. The results show that the MMGBSA approach is able to reproduce experimental trends and identify eight residues that strongly interact with the ligands.