Article
Biochemistry & Molecular Biology
Guoxing Xu, Azra Fatima, Martin Breitbach, Alexey Kuzmenkin, Christopher J. Fuegemann, Dina Ivanyuk, Kee Pyo Kim, Tobias Cantz, Kurt Pfannkuche, Hans R. Schoeler, Bernd K. Fleischmann, Juergen Hescheler, Tomo Saric
Summary: In this study, tetraploid pluripotent stem cells (PSCs) were successfully generated by fusion of mouse embryonic stem cells and somatic cells. The electrophysiological properties of the tetraploid fusion-derived PSCs and diploid ESC-derived cardiomyocytes (CMs) were compared. The results showed that the structural and electrophysiological properties of the two types of CMs were similar.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Cardiac & Cardiovascular Systems
Mengcheng Shen, Thomas Quertermous, Michael P. Fischbein, Joseph C. Wu
Summary: The developmental origin of vascular smooth muscle cells plays a crucial role in regional susceptibility and resistance to vascular diseases. Patient induced pluripotent stem cell-derived VSMCs are valuable for research and therapeutic applications, but the heterogeneity in developmental origins poses challenges.
CIRCULATION RESEARCH
(2021)
Article
Biochemistry & Molecular Biology
Mina Ishioka, Yuma Nihashi, Yoichi Sunagawa, Koji Umezawa, Takeshi Shimosato, Hiroshi Kagami, Tatsuya Morimoto, Tomohide Takaya
Summary: This study found that inhibition of nucleolin by iSN04 can promote the differentiation of cardiac mesoderm into cardiomyocytes but interferes with the differentiation of early mesoderm into the cardiac lineage. This is the first report on generating cardiomyocytes from pluripotent stem cells using a DNA aptamer. iSN04 does not induce hypertrophic responses in primary-cultured cardiomyocytes, making it useful and safe for regenerative therapy of heart failure using stem cell-derived cardiomyocytes.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Engineering, Biomedical
Jiesi Luo, Yuyao Lin, Xiangyu Shi, Guangxin Li, Mehmet H. Kural, Christopher W. Anderson, Matthew W. Ellis, Muhammad Riaz, George Tellides, Laura E. Niklason, Yibing Qyang
Summary: Research successfully derived functional xenogeneic-free hiPSC-VSMCs by replacing animal-derived reagents with functional counterparts of human origin, and developed tissue rings with comparable mechanical strength. Engineered vascular tissues were generated by seeding XF-hiPSC-VSMCs onto biodegradable PGA scaffolds, suitable for implantation into an immunodeficient mice model.
ACTA BIOMATERIALIA
(2021)
Review
Biochemistry & Molecular Biology
Yong-Jin Kim, Amin Tamadon, Yoon-Young Kim, Byeong-Cheol Kang, Seung-Yup Ku
Summary: This review focuses on the current knowledge of epigenetic regulation of cardiomyocyte proliferation and differentiation from embryonic and induced pluripotent stem cells through histone modification and microRNAs, the maintenance of pluripotency, and its alteration during cardiac lineage differentiation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Developmental Biology
Andrea L. Bredemeyer, Junedh M. Amrute, Andrew L. Koenig, Rachel A. Idol, Li He, Stephanie A. Luff, Carissa Dege, Jamison M. Leid, Joel D. Schilling, J. Travis Hinson, Mary C. Dinauer, Christopher M. Sturgeon, Kory J. Lavine
Summary: Tissue-resident macrophages play a crucial role in organ homeostasis, tissue repair, remodeling, and regeneration. Researchers have developed a system to generate macrophages that resemble tissue-resident or monocyte-derived subsets, allowing for the study of their function and potential applications in tissue engineering and regenerative medicine.
Review
Biochemistry & Molecular Biology
Yahong Wu, Weiwei Zhang
Summary: Pluripotent stem cells, including ESCs and iPSCs, are crucial for studying early development and treating diseases, with ubiquitination playing a key role in pluripotency regulation, particularly through the actions of E3 ligases.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Peripheral Vascular Disease
Lu Liu, Charlene Jouve, Josephine Henry, Takiy-Eddine Berrandou, Jean-Sebastien Hulot, Adrien Georges, Nabila Bouatia-Naji
Summary: This study characterized the cellular phenotypes, epigenomic and proteomic profiles of smooth muscle cells (SMCs) derived from induced pluripotent stem cells (iPSCs) and evaluated their potential as cellular models for complex diseases. The iPSC-derived SMCs demonstrated different functional properties and gene expression profiles. These cellular models have the potential to investigate the functional regulation of genetic risk loci in major arterial diseases.
Article
Cell & Tissue Engineering
Shuang Zhao, Chuanyu Zhang, Jia Xu, Siying Liu, Lu Yu, Shang Chen, Hang Wen, Zongjin Li, Na Liu
Summary: This study identified potential interacting proteins with Dppa3 and found that Dppa3 safeguards the stability of Uhrf1 and Nanog by inhibiting proteasome-associated degradation in ES cells.
STEM CELL RESEARCH & THERAPY
(2022)
Article
Biology
James Z. J. Kwan, Thomas F. Nguyen, Anuli C. Uzozie, Marek A. Budzynski, Jieying Cui, Joseph M. C. Lee, Filip Van Petegem, Philipp F. Lange, Sheila S. Teves
Summary: Depletion of TBP in mouse embryonic stem cells does not affect ongoing Pol II transcription, but severely impairs Pol III initiation. However, Pol II transcriptional induction still occurs normally upon TBP depletion. This TBP-independent transcription mechanism is not due to functional redundancy with TRF2, but rather the robustness of the TFIID complex and the Pol II machinery.
Article
Biochemistry & Molecular Biology
Julian O. Kimura, Marcela Bolanos, Lorenzo Ricci, Mansi Srivastava
Summary: This study reveals the cellular mechanism and molecular trajectory for the formation of adult pluripotent stem cells (aPSCs) during embryonic development. By utilizing lineage tracing and single-cell transcriptome profiling, the researchers identified a molecular trajectory for neoblast formation and showed that aPSCs derive from one lineage instead of multiple tissue-specific lineages.
Article
Engineering, Biomedical
Fengzhi Zhang, Yonglin Zhu, Jing Chen, Wenhua Kuang, Rujin Huang, Fuyu Duan, Yaqian Li, Lin Wang, Hui Qiu, Xia Chen, Jia Ming, Peng Liu, Yanan Du, Sophia Chia-Ning Chang, Ligong Chen, Jie Na
Summary: The study identified a simplified and chemically defined medium AATS that can help differentiate human pluripotent stem cells into endothelial cells and smooth muscle cells quickly and efficiently, showing potential in vascular cell therapy.
Article
Cardiac & Cardiovascular Systems
Samantha K. Atkins, Abhijeet R. Sonawane, Romi Brouwhuis, Johana Barrientos, Anna Ha, Maximillian Rogers, Takeshi Tanaka, Takehito Okui, Shiori Kuraoka, Sasha A. Singh, Masanori Aikawa, Elena Aikawa
Summary: Cardiovascular calcification is a major predictor of cardiovascular events and a leading cause of global morbidity and mortality. This study presents a model of induced pluripotent stem cell-derived smooth muscle cells (iSMCs) for studying the pathogenesis of vascular calcification. The study successfully differentiated iPSCs into iSMCs and induced calcification in the iSMCs, providing an in vitro model for future research on cardiovascular calcification.
FRONTIERS IN CARDIOVASCULAR MEDICINE
(2022)
Article
Cell Biology
Kimberley M. Reid, Juan Miguel Sanchez-Nieto, Sandra Terrasse, Danilo Faccenda, Barbara Pernaute, Michelangelo Campanella, Tristan A. Rodriguez, Bradley S. Cobb
Summary: MicroRNAs (miRNAs) play a crucial role in regulating embryonic stem cell (ESC) biology and have been found to regulate various pathways, including Ca2+ signaling. In this study, the absence of miRNAs due to Dicer deletion resulted in increased responsiveness of ESCs to thapsigargin-induced Ca2+ import into the endoplasmic reticulum (ER). This increase in Ca2+ response was also observed during purinergic receptor activation and was found to involve the upregulation of one isoform of the IP3 receptors (Itpr2), primarily through transcriptional regulation.
Article
Cell & Tissue Engineering
Jin Ju Park, Yang Woo Kwon, Jeong Won Kim, Gyu Tae Park, Jung Won Yoon, Ye Seul Kim, Da Sol Kim, Sang Mo Kwon, Sun Sik Bae, Kinarm Ko, Chang-Seok Kim, Jae Ho Kim
Summary: The study demonstrated the therapeutic potential of co-transplanting iPSC-derived ECs and SMCs in peripheral artery disease, promoting angiogenesis and increasing limb salvage rate.
STEM CELLS TRANSLATIONAL MEDICINE
(2021)
Article
Medicine, Research & Experimental
Olurotimi O. Mesubi, Adam G. Rokita, Neha Abrol, Yuejin Wu, Biyi Chen, Qinchuan Wang, Jonathan M. Granger, Anthony Tucker-Bartley, Elizabeth D. Luczak, Kevin R. Murphy, Priya Umapathi, Partha S. Banerjee, Tatiana N. Boronina, Robert N. Cole, Lars S. Maier, Xander H. Wehrens, Joel L. Pomerantz, Long-Sheng Song, Rexford S. Ahima, Gerald W. Hart, Natasha E. Zachara, Mark E. Anderson
Summary: Diabetes mellitus (DM) and atrial fibrillation (AF) are significant public health issues, with diabetes being an independent risk factor for AF. This study found that ROS and protein O-GlcNAcylation (OGN) play a role in the development of diabetic AF, with CaMKII identified as a critical proarrhythmic signal.ROS primarily promotes AF through oxidized CaMKII, while OGN promotes AF through a CaMKII-independent mechanism.
JOURNAL OF CLINICAL INVESTIGATION
(2021)
Article
Biochemistry & Molecular Biology
Jinxi Wang, Grace Ciampa, Dong Zheng, Qian Shi, Biyi Chen, E. Dale Abel, Tianqing Peng, Duane D. Hall, Long-Sheng Song
Summary: Calpain proteolysis, particularly by the isoforms CAPN1 and CAPN2, contributes to heart failure pathogenesis through cleavage of JP2. While both isoforms are induced by pressure overload stress, CAPN1 shows higher calcium sensitivity and cleavage efficacy compared to CAPN2, suggesting potential targeted therapy for heart failure by specific inhibition of CAPN1.
BIOCHEMICAL JOURNAL
(2021)
Article
Biology
Xuhui Han, Bingqi Wang, Dongxu Jin, Kuang Liu, Hongjie Wang, Liangbiao Chen, Yao Zu
Summary: Folic acid is an essential vitamin and its supplementation during pregnancy is crucial for embryonic development. This study demonstrates the impact of excessive or insufficient folic acid intake on cardiac development in zebrafish embryos and establishes a zebrafish mutant model for studying folate metabolism. The findings emphasize the importance of precise folic acid dosage for embryonic development.
Article
Cardiac & Cardiovascular Systems
Jinxi Wang, Qian Shi, Yihui Wang, Logan W. Dawson, Grace Ciampa, Weiyang Zhao, Guangqin Zhang, Biyi Chen, Robert M. Weiss, Chad E. Grueter, Duane D. Hall, Long-Sheng Song
Summary: This study demonstrates the potential of JP2NT gene therapy in attenuating transcriptional remodeling and development of heart failure induced by cardiac stress. The findings suggest that JP2NT gene therapy may serve as a novel therapeutic approach for treating hypertrophy and heart failure.
CIRCULATION RESEARCH
(2022)
Article
Biophysics
Liping Yu, Duane D. Hall, Weiyang Zhao, Long-Sheng Song
Summary: JP2 protein plays a crucial role in cardiac function, and its DNA-binding domain is an intrinsically disordered protein that binds to DNA, influencing gene transcriptional regulation and the stabilization of junctional membrane complexes.
BIOMOLECULAR NMR ASSIGNMENTS
(2022)
Article
Genetics & Heredity
Qianqian Yan, Wenhao Li, Xiaoting Gong, Ruiqin Hu, Liangbiao Chen
Summary: In this study, GluK2 gene was found to be highly expressed in the brains and eyes of adult zebrafish and play a crucial role in cold stress. Gene knockout of GluK2 using the CRISPR/Cas9 system was performed, and differentially expressed genes related to the response to temperature stimulus pathway were identified through RNA-seq analysis. Behavioral phenotyping assay and TUNEL staining confirmed the necessity of GluK2 for cold tolerance in zebrafish.
Article
Oncology
Jin-Young Yoon, Nastaran Daneshgar, Yi Chu, Biyi Chen, Marco Hefti, Ajit Vikram, Kaikobad Irani, Long-Sheng Song, Charles Brenner, E. Dale Abel, Barry London, Dao-Fu Dai
Summary: This study reveals the mechanistic explanations for cardiac bradyarrhythmia, diastolic dysfunction, and neuronal apoptosis observed in models of Leigh syndrome (LS).
CLINICAL AND TRANSLATIONAL MEDICINE
(2022)
Article
Chemistry, Multidisciplinary
Xiao-yun Ji, Dong Zheng, Rui Ni, Jin-xi Wang, Jian-qiang Shao, Zer Vue, Antentor Hinton, Long-Sheng Song, Guo-Chang Fan, Subrata Chakrabarti, Zhao-liang Su, Tian-qing Peng
Summary: This study reveals that sustained over-expression of calpain-2 induces age-dependent dilated cardiomyopathy in mice, possibly through aberrant autophagy and a reduction of junctophilin-2. Inhibiting autophagy can attenuate myocardial injury and improve heart function.
ACTA PHARMACOLOGICA SINICA
(2022)
Editorial Material
Cardiac & Cardiovascular Systems
Duane D. Hall, Qian Shi, Long-Sheng Song
CIRCULATION RESEARCH
(2022)
Article
Fisheries
Yueyue Fei, Qin Wang, Jigang Lu, Linyue Ouyang, Wei Li, Ruiqin Hu, Liangbiao Chen
Summary: In this study, the researchers cloned the LEAP2 coding sequence from C. hamatus and found high levels of LEAP2 mRNA expression in the skin and liver. The mature peptide of LEAP2 demonstrated selective antimicrobial activities against various bacteria and showed bactericidal activity by destroying the cell membrane integrity and binding with bacterial genomic DNA. Overexpression of Tol-LEAP2-EGFP in zebrafish larva enhanced antimicrobial activity and reduced bacterial load and expression of pro-inflammatory factors. This research provides valuable insights into the antimicrobial activity of LEAP2 from C. hamatus and its potential role in enhancing resistance to pathogens.
JOURNAL OF FISH DISEASES
(2023)
Article
Environmental Sciences
Yihan Yu, Lihua Hu, Dandan Tian, Yingying Yu, Lingzheng Lu, Jiongming Zhang, Xianke Huang, Maocang Yan, Liangbiao Chen, Zhichao Wu, Wei Shi, Guangxu Liu
Summary: This study evaluated the effects of microplastics (MPs) and hexabromocyclododecane (HBCD), alone or in combination, on the hepatopancreas of whiteleg shrimp. The results showed evident histological injury, elevation in hepatic lesion markers, disruption of metabolism and detoxification processes, alterations in antioxidant enzymes, and oxidative damage to the hepatopancreas. Furthermore, accumulation of HBCD in shrimp and increased toxicity of HBCD-MP coexposure were observed.
ENVIRONMENTAL POLLUTION
(2023)
Article
Fisheries
Yan Zhou, Yanjie Zhang, Shang Wei, Wei Li, Wenhao Li, Zhichao Wu, Shouwen Jiang, Ying Lu, Qianghua Xu, Liangbiao Chen
Summary: This study found that long-term exposure to high temperatures has detrimental effects on the survival and metabolism of Nile tilapia. Fish reared at 36 degrees C showed significantly reduced survival rates and tolerance to hypoxia, as well as increased respiratory rates. After 15 weeks at 36 degrees C, a partial temperature compensation for routine metabolic rates was observed.
Article
Fisheries
Tao Zhou, Zhihua Fang, Daniel F. C. Duarte, Stefan A. Fernandes, Ying Lu, Jing Guo, Lang Gui, Liangbiao Chen
Summary: This study investigated the mechanisms of gill damage in tilapia infected with Streptococcus agalactiae. Transcriptomic analysis revealed differentially expressed genes and enriched immune-related gene ontology and pathways. HE staining results showed severe gill damage in tilapia at 9 hours post-infection.
Article
Fisheries
Carmen S. V. Sousa, Deborah M. Power, Pedro M. Guerreiro, Bruno Louro, Liangbiao Chen, Adelino V. M. Canario
Summary: The environmental conditions and isolation in the Antarctic have driven the evolution of a unique biodiversity. This study investigates the possible adaptation of the Notothenia coriiceps immune system to the cold environment and unique microbial community of the Southern Ocean. The findings suggest that the immune processes in N. coriiceps differ from other teleost fish.
Article
Fisheries
Shuang Han, Shang Wei, Ruoyu Chen, Man Ni, Liangbiao Chen
Summary: This study provides a model for understanding the response mechanisms of fish under cold stress by studying the physiological and behavioral changes in low-temperature domesticated fish. The results show that cold-tolerant fugu reduces brain tissue damage and copes with cold environment by employing a dynamic metabolic regulation strategy.