Article
Biochemistry & Molecular Biology
Yining Zhang, Yixin Zhao, Zhengsong Xie, Molin Li, Yujiao Liu, Xiaolin Tu
Summary: Our study confirmed that BMP-7 mediates the anabolic actions of Wnt/β-catenin signaling in osteocytes. This provides new evidence for the clinical application of BMP-7-overexpressed osteocytes.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biology
Sanjana Rajan, Emily M. M. Franz, Camille A. A. McAloney, Tatyana A. A. Vetter, Maren Cam, Amy C. C. Gross, Cenny Taslim, Meng Wang, Matthew V. V. Cannon, Alexander Oles, Ryan D. D. Roberts
Summary: Tumors are complex tissues that contain diverse malignant and nonmalignant cells. The mechanisms governing tumor cell heterogeneity and its role in adapting to different microenvironments are poorly understood. Osteosarcoma is an ideal model for studying these mechanisms due to its widespread heterogeneity, predictable metastasis patterns, and lack of clear driver mutations. Understanding how tumor cells adapt to primary and metastatic microenvironments can inform the development of targeted therapies.
Article
Gastroenterology & Hepatology
Hiroki Kobayashi, Krystyna A. Gieniec, Josephine A. Wright, Tongtong Wang, Naoya Asai, Yasuyuki Mizutani, Tadashi Lida, Ryota Ando, Nobumi Suzuki, Tamsin R. M. Lannagan, Jia Q. Ng, Akitoshi Hara, Yukihiro Shiraki, Shinji Mii, Mari Ichinose, Laura Vrbanac, Matthew J. Lawrence, Tarik Sammour, Kay Uehara, Gareth Davies, Leszek Lisowski, Ian E. Alexander, Yoku Hayakawa, Lisa M. Butler, Andrew C. W. Zannettino, M. Omar Din, Jeff Hasty, Alastair D. Burt, Simon J. Leedham, Anil K. Rustgi, Siddhartha Mukherjee, Timothy C. Wang, Atsushi Enomoto, Masahide Takahashi, Daniel L. Worthley, Susan L. Woods
Summary: The study identified Grem1 and Islr as CAF-specific genes involved in BMP signaling. High expression levels of GREM1 and ISLR in patients with CRC were associated with poor and favorable survival, respectively. Targeting GREM1 with a neutralizing antibody or overexpressing Islr in fibroblasts can reduce CRC tumoroid growth and promote Lgr5(+) intestinal stem cell differentiation.
Review
Cell Biology
Caterina Cascini, Claudia Chiodoni
Summary: Osteosarcoma is a high-grade malignant stromal tumor with peak incidence in the second decade of life. Despite being relatively rare, understanding its molecular genetics and tumor microenvironment has been limited, but recent advancements have shed new light on these aspects. This knowledge is crucial for developing novel therapeutic strategies and improving disease prognosis and treatment response.
Review
Cell Biology
Paul Takam Kamga, Riccardo Bazzoni, Giada Dal Collo, Adriana Cassaro, Ilaria Tanasi, Anna Russignan, Cristina Tecchio, Mauro Krampera
Summary: The Notch and Wnt signaling pathways are important for hematopoiesis and leukemia, but their exact contributions are still debated. Recent research highlights the critical role played by MSC-derived Notch and Wnt signaling in the development of hematopoiesis and leukemia.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Ana Carolina Tirado-Garibay, Elba Andrea Falcon-Ruiz, Alejandra Ochoa-Zarzosa, Joel E. Lopez-Meza
Summary: GPER is a receptor associated with cancer, with tissue-specific activity that can promote or inhibit tumor growth. It plays an important role in epithelial-mesenchymal transition and contributes to tumor dissemination and maintenance in the tumor microenvironment. Therefore, GPER could be a promising therapeutic target.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Engineering, Biomedical
Zhong Li, Shiqi Xiang, Zixuan Lin, Eileen N. Li, Haruyo Yagi, Lauren Yocum, La Li, Katherine K. Bruce, Madalyn R. Fritch, Huanlong Hu, Bing Wang, Peter G. Alexander, Khiam Aik Khor, Rocky S. Tuan, Hang Lin
Summary: This study successfully enhanced the osteogenic capability of hMSCs by incorporating graphene oxide-based nanosheets into GelMA. Both in vitro and in vivo experiments demonstrated the tremendous potential of this technology, providing new possibilities for bone tissue engineering applications.
Review
Biochemistry & Molecular Biology
Kurt Engeland
Summary: RB and p53 are central tumor suppressors that play key roles in regulating the cell division cycle. RB downregulates numerous genes by forming complexes with E2F transcription factors, and its repression is released through phosphorylation. p53 activates CDKN1A gene to regulate RB phosphorylation, leading to expression of cell cycle regulators. There is an overlap between RB and DREAM signaling pathways.
CELL DEATH AND DIFFERENTIATION
(2022)
Review
Medicine, General & Internal
Jichao Bian, Yang Liu, Xiaowei Zhao, Chunyang Meng, Yuanmin Zhang, Yangmiao Duan, Guodong Wang
Summary: This review discusses the mechanisms of osteosarcoma development, identifies therapeutic targets, and summarizes available treatment modalities, while also highlighting future research priorities.
CHINESE MEDICAL JOURNAL
(2023)
Review
Cell Biology
Forough Alemi, Faezeh Malakoti, Mostafa Vaghari-Tabari, Jafar Soleimanpour, Nazila Shabestani, Aydin R. Sadigh, Nafiseh Khelghati, Zatollah Asemi, Yasin Ahmadi, Bahman Yousefi
Summary: Osteosarcoma is a common bone malignancy with a low survival rate, especially in advanced stages. Resistance to chemotherapy, mainly through increased DNA repair ability, is a major obstacle in the treatment of osteosarcoma. Inhibiting the DNA damage response pathway may reduce chemoresistance and improve the effectiveness of chemotherapy.
JOURNAL OF CELLULAR PHYSIOLOGY
(2022)
Article
Oncology
Yang Zhou, Jiang Xu, Haichang Luo, Xiangjing Meng, Ming Chen, Di Zhu
Summary: Abnormal activation of the Wnt/beta-catenin signaling pathway is closely related to tumorigenesis and immune surveillance, leading to increased resistance to immunotherapy.
Article
Oncology
Zhitao Han, Lining Wang, Dongshuo Wang, Luosheng Zhang, Yifeng Bi, Xinlei Zheng, Weibo Liu, Guangjian Bai, Zhenhua Wang, Wei Wan, Yong Ma, Xiaopan Cai, Tielong Liu, Qi Jia
Summary: This study found that abnormal expression of DJ-1 gene is associated with the prognosis of osteosarcoma patients. DJ-1 can affect the occurrence and progression of osteosarcoma by regulating the CDK/RB/E2F1 axis, providing a new therapeutic opportunity for osteosarcoma patients.
FRONTIERS IN ONCOLOGY
(2022)
Review
Oncology
Yi-Ming Gao, Yan Pei, Fei-Fei Zhao, Ling Wang
Summary: This review examines the physiological characteristics and functional mechanisms of osteoclasts in osteosarcoma, as well as their interaction with other cell types. It emphasizes the significance of osteoclasts in the onset and progression of osteosarcoma and discusses potential therapies targeting osteoclasts for treatment.
CANCER MANAGEMENT AND RESEARCH
(2023)
Article
Biochemistry & Molecular Biology
Zhourui Ma, Shizhong Cai, Qianwei Xiong, Wei Liu, Hongliang Xia, Zhenhong Zhu, Zhijian Huang, Xiangming Yan, Qian Wang
Summary: This study reveals the role of WNT signaling in the chemoresistance of glioblastoma multiforme (GBM) to temozolomide (TMZ). The loss of function of p53 in GBM downregulates miR-34a expression, leading to the activation of WNT signaling and the resistance to TMZ. By targeting the miR-34a/WNT6 axis, drug sensitivity of p53-mutant GBM cells can be restored and overall survival can be extended.
Article
Biochemistry & Molecular Biology
Vijesh G. Vaghjiani, Catherine R. Cochrane, W. Samantha N. Jayasekara, Wai Chin Chong, Anette Szczepny, Beena Kumar, Luciano G. Martelotto, Andrew Mccaw, Kirstyn Carey, Maya Kansara, David M. Thomas, Carl Walkley, Stuart Mudge, Daniel J. Gough, Peter A. Downie, Craig D. Peacock, William Matsui, D. Neil Watkins, Jason E. Cain
Summary: TP53 and RB1 loss-of-function mutations are common in osteosarcoma. In this study, the researchers found that these mutations enhance the activation of the Hedgehog (Hh) signaling pathway. This enhanced activation is mediated by hypersensitivity to Hh ligand and is accompanied by impaired autophagy, increased primary cilia formation, and expression of Hh ligand.
Article
Oncology
Elena Llano, Anne Laure Todeschini, Natalia Felipe-Medina, Maria D. Corte-Torres, Yazmine B. Condezo, Manuel Sanchez-Martin, Sara Lopez-Tamargo, Aurora Astudillo, Xose S. Puente, Alberto M. Pendas, Reiner A. Veitia
Summary: This study utilized a mouse model carrying a FOXL2 gene mutation to demonstrate that FOXL2 C134W mutation is associated with the development of malignant ovarian tumors, and the analysis of transcriptomic data suggests that FOXL2-C134W is the only driver mutation for tumor development.
Letter
Hematology
Clara Bueno, Alba Martinez, Paola Alejandra Romecin, Samanta Romina Zanetti, Ricky Tirtakusuma, Eulalia Genesca, Mireia Camos, Manuel Ramirez-Orellana, Eduardo Anguita, Paola Ballerini, Franco Locatelli, Jose Luis Fuster, Pablo Menendez
Article
Multidisciplinary Sciences
Laureano Tomas-Daza, Llorenc Rovirosa, Paula Lopez-Marti, Andrea Nieto-Aliseda, Francois Serra, Ainoa Planas-Riverola, Oscar Molina, Rebecca McDonald, Cedric Ghevaert, Esther Cuatrecasas, Dolors Costa, Mireia Camos, Clara Bueno, Pablo Menendez, Alfonso Valencia, Biola M. Javierre
Summary: In this study, the authors present a cost-effective and flexible method called liCHi-C to map and compare promoter interactomes at high resolution. They demonstrate its broad applicability by studying normal and malignant human hematopoietic hierarchy and show that it can identify developmental trajectories and disease-relevant cell types, genes, and pathways. Additionally, liCHi-C can be used to uncover genome-wide structural variants and infer their pathogenic effects.
NATURE COMMUNICATIONS
(2023)
Review
Biochemistry & Molecular Biology
Dzohara Murillo, Carmen Huergo, Borja Gallego, Rene Rodriguez, Juan Tornin
Summary: Drug resistance is a major problem in cancer treatment, and cold atmospheric plasma (CAP) shows promise in overcoming it. CAP uses ionized gas to generate reactive species that can kill cancer cells. This review explores the mechanisms behind CAP's effectiveness and its potential applications in cancer treatment. Further research is needed to address the challenges and opportunities in this field.
Article
Medicine, Research & Experimental
Oscar Estupinan, Veronica Rey, Juan Tornin, Dzohara Murillo, Borja Gallego, Carmen Huergo, Veronica Blanco-Lorenzo, M. Victoria Gonzalez, Aida Rodriguez, Francisco Moris, Jessica Gonzalez, Veronica Ayllon, Veronica Ramos-Mejia, Anna Bigas, Rene Rodriguez
Summary: EC-8042 is a well-tolerated mithramycin analog that efficiently eliminates tumor cells and cancer stem cell subpopulations in osteosarcomas by repressing the NOTCH1 signaling pathway.
BIOMEDICINE & PHARMACOTHERAPY
(2023)
Article
Medicine, Research & Experimental
Rocio Granda-Diaz, Lorea Manterola, Francisco Hermida-Prado, Rene Rodriguez, Laura Santos, Vanessa Garcia-de-la-Fuente, Maria Teresa Fernandez, M. Daniela Corte-Torres, Juan P. Rodrigo, Saul Alvarez-Teijeiro, Charles H. Lawrie, Juana M. Garcia-Pedrero
Summary: Treatment of head and neck squamous cell carcinomas (HNSCC) remains challenging. Dysregulation of miRNA is closely linked to tumorigenesis and tumor progression, making it a suitable target for cancer treatment. In this study, miR-301a was found to be overexpressed in HNSCC tumors and was shown to play a role in promoting tumor growth and invasion. The PI3K/PTEN/AKT and MEK/ERK pathways were identified as central signaling nodes responsible for mediating the oncogenic activity of miR-301a.
BIOMEDICINE & PHARMACOTHERAPY
(2023)
Letter
Hematology
Oskar Marin-Bejar, Damia Romero-Moya, Javier Rodriguez-Ubreva, Maximiliano Distefano, Francesca Lessi, Paolo Aretini, Alessandro Liquori, Julio Castano, Emilia Kozyra, Lili Kotmayer, Clara Bueno, Jose Cervera, Jose Carlos Rodriguez-Gallego, Josep F. Nomdedeu, Laura Murillo-Sanjuan, Cristina Diaz de Heredia, Antonio Perez-Martinez, Felix Lopez-Cadenas, Carolina Martinez-Laperche, Nieves Dorado-Herrero, Francisco M. Marco, Felipe Prosper, Pablo Menendez, David Valcarcel, Esteban Ballestar, Csaba Bodor, Anna Bigas, Albert Catala, Marcin W. Wlodarski, Alessandra Giorgetti
Article
Oncology
C. Meyer, P. Larghero, B. Almeida Lopes, T. Burmeister, D. Groeger, R. Sutton, N. C. Venn, G. Cazzaniga, L. Corral Abascal, G. Tsaur, L. Fechina, M. Emerenciano, M. S. Pombo-de-Oliveira, T. Lund-Aho, T. Lundan, M. Montonen, V. Juvonen, J. Zuna, J. Trka, P. Ballerini, H. Lapillonne, V. H. J. van der Velden, E. Sonneveld, E. Delabesse, R. R. C. de Matos, M. L. M. Silva, S. Bomken, K. Katsibardi, M. Keernik, N. Grardel, J. Mason, R. Price, J. Kim, C. Eckert, L. Lo Nigro, C. Bueno, P. Menendez, U. zur Stadt, P. Gameiro, L. Sedek, T. Szczepanski, A. Bidet, V. Marcu, K. Shichrur, S. Izraeli, H. O. Madsen, B. W. Schaefer, S. Kubetzko, R. Kim, E. Clappier, H. Trautmann, M. Brueggemann, P. Archer, J. Hancock, J. Alten, A. Moericke, M. Stanulla, J. Lentes, A. K. Bergmann, S. Strehl, S. Koehrer, K. Nebral, M. N. Dworzak, O. A. Haas, C. Arfeuille, A. Caye-Eude, H. Cave, R. Marschalek
Summary: This study analyzed the genomic breakpoints in 3401 acute leukemia patients and identified 107 KMT2A gene fusions and other rearrangements. The study also found seven common fusion genes and partial tandem duplications, which are important for understanding the pathogenesis of acute leukemia and monitoring minimal residual disease.
Article
Multidisciplinary Sciences
Nadia Mensali, Hakan Koeksal, Sandy Joaquina, Patrik Wernhoff, Nicholas P. Casey, Paola Romecin, Carla Panisello, Rene Rodriguez, Lene Vimeux, Asta Juzeniene, Marit R. Myhre, Anne Fane, Carolina Castilla Ramirez, Solrun Melkorka Maggadottir, Adil Doganay Duru, Anna-Maria Georgoudaki, Iwona Grad, Andres Daniel Maturana, Gustav Gaudernack, Gunnar Kvalheim, Angel M. Carcaboso, Enrique de Alava, Emmanuel Donnadieu, Oyvind S. Bruland, Pablo Menendez, Else Marit Inderberg, Sebastien Waelchli
Summary: Osteosarcoma remains a challenging malignancy with limited immunotherapy options. However, this study demonstrates that chimeric antigen receptor (CAR) T cells targeting the ALPL-1 isoform of alkaline phosphatase show high efficiency and specificity in preclinical models of primary and metastatic osteosarcoma. These CAR-T cells display potent cytotoxicity against ALPL-positive cells without unexpected toxicities, providing a promising path for clinical translation of CAR-T immunotherapy in osteosarcoma.
NATURE COMMUNICATIONS
(2023)
Article
Biochemistry & Molecular Biology
Juan Tornin, Miguel Mateu-Sanz, Veronica Rey, Dzohara Murillo, Carmen Huergo, Borja Gallego, Aida Rodriguez, Rene Rodriguez, Cristina Canal
Summary: Osteosarcoma is a malignant bone cancer with poor prognosis, partly due to drug-resistant cancer stem cells. Cold atmospheric plasmas and plasma-treated liquids (PTL) have shown selectivity against cancer cells, but their effects on cancer stem cells and in vivo tumor growth are not well understood. This study demonstrates that low doses of PTL promote stemness factors and self-renewal ability of osteosarcoma cells, leading to enhanced tumor growth in vivo. However, combining PTL with a STAT3 inhibitor can suppress tumor growth effectively, suggesting a potential treatment for osteosarcoma.
Article
Veterinary Sciences
Emma Deards, Anita Economou, Pablo Menendez
Summary: This case describes a presumed traumatic fragmented medial coronoid process in a cat, which was successfully treated with surgical removal of the fragment. The patient improved postoperatively, but still experienced intermittent lameness after periods of exertion.
VETERINARY RECORD CASE REPORTS
(2023)
Article
Biochemistry & Molecular Biology
Juan Tornin, Borja Gallego, Veronica Rey, Dzohara Murillo, Carmen Huergo, Aida Rodriguez, Cristina Canal, Rene Rodriguez
Summary: This study reveals the potential of Cold Plasma Treated Medium (PTM) in preventing doxorubicin (DX) resistance in osteosarcoma (OS). PTM treatment increases oxidative stress levels in DX-resistant cells and sensitizes them to DX.
FREE RADICAL BIOLOGY AND MEDICINE
(2023)
Article
Materials Science, Biomaterials
Raul Sanz-Horta, Sugoi Retegi-Carrion, Raquel Ruiz-Hernandez, Neda Khatami, Carlos Elvira, Enrique Martinez-Campos, Juan Rodriguez-Hernandez, Ander Abarrategi
Summary: To overcome the challenge of fabricating tailor-made porous scaffolds for biomedical applications, researchers used multimaterial fused deposition modeling to generate precise PCL 3D objects by filling PVA sacrificial molds. Supercritical CO2 and breath figures mechanisms were employed to create specific porous structures at the surface and core of the PCL object, respectively. The biocompatibility of the resulting multiporous 3D structures was tested and the versatility of the approach was demonstrated by creating a fully tunable vertebra model with multiple pore size levels.
BIOMATERIALS ADVANCES
(2023)
Article
Oncology
Alvaro Morales-Molina, Miguel angel Rodriguez-Milla, Stefano Gambera, Teresa Cejalvo, Belen de Andres, Maria-Luisa Gaspar, Javier Garcia-Castro
Summary: Cancer immunotherapy aims to activate the immune system and deliver immunotherapeutic agents to tumors using carrier cells. This study found that using carrier cells with a low proinflammatory profile resulted in better tumor homing and improved antitumor responses, highlighting the importance of selecting appropriate cells for cell-based therapies in cancer treatment.
CANCER RESEARCH COMMUNICATIONS
(2023)
