Article
Dermatology
Xuejun Gao, Chao Lu, Yuanxin Miao, Jizhen Ren, Xia Cai
Summary: This study aimed to investigate the roles of M1 and M2 macrophages in skin wound healing in order to develop new drugs for refractory wounds. Primary bone marrow-derived macrophages (BMDMs) were isolated and polarized into M1 and M2 subtypes. The results showed that M2 cells promoted wound healing, while M1 cells delayed the healing process.
INTERNATIONAL WOUND JOURNAL
(2023)
Article
Biochemistry & Molecular Biology
Wen-qing Tian, Si-yu Chen, Feng-ning Chuan, Wen-rui Zhao, Bo Zhou
Summary: TINAGL1 plays an important role in diabetic wound healing and its expression is regulated by high glucose. Administration of exogenous TINAGL1 can promote wound healing in diabetic mice. These findings provide new insights for the treatment of diabetic wounds.
Article
Biochemistry & Molecular Biology
Chenhui Dong, Anne Gingery, Peter C. Amadio, Kai-Nan An, Steven L. Moran, Chunfeng Zhao
Summary: The study found that apoptotic bodies from injured tenocytes play a critical role in promoting cell proliferation, migration, and extracellular matrix synthesis for tendon healing. Further research is needed to analyze the components of apoptotic bodies for developing targeted therapeutics.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Cheng-Chang Lu, Cheng-Jung Ho, Hsuan-Ti Huang, Sung-Yen Lin, Shih-Hsiang Chou, Pei-Hsi Chou, Mei-Ling Ho, Yin-Chun Tien
Summary: Both bone marrow mononuclear cells (BMMNCs) and bone marrow stromal cells (BMSCs) have comparable effects on cell repopulation and interfacial allograft-bone healing after allograft anterior cruciate ligament reconstruction (ACLR). Intra-articular delivery of BM cells has limited benefits on graft cell repopulation and may cause higher inflammation in the early stage, with fibrocartilage formation in the tendon-bone interface.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Kento Takaya, Ayano Sunohara, Noriko Aramaki-Hattori, Shigeki Sakai, Keisuke Okabe, Kazuo Kishi
Summary: Multiple transitions occur in the healing ability of the skin during embryonic development in mice. Lhx2 is a regulator involved in the activation and maintenance of hair follicle stem cells. Analysis of surgically wounded mouse embryos revealed that Lhx2 expression is associated with cell division and scar formation.
Article
Biochemistry & Molecular Biology
Masaya Yokota, Nicola Haeffner, Matthew Kassier, Matthias Brunner, Srikanth Mairpady Shambat, Fabian Brennecke, Janine Schniering, Ewerton Marques Maggio, Oliver Distler, Annelies Sophie Zinkernagel, Britta Maurer
Summary: The study revealed that Staphylococcus aureus affects dermal fibroblasts, leading to impaired tissue repair functions such as upregulation of pro-inflammatory cytokines and degradation of collagen. This impact is not limited to specific cell sources and affects key mechanisms of wound healing.
Article
Pharmacology & Pharmacy
Victor Gustavo Balera Brito, Mariana Sousa Patrocinio, Ayna Emanuelli Alves Barreto, Sabrina Cruz Tfaile Frasnelli, Vanessa Soares Lara, Carlos Ferreira Santos, Sandra Helena Penha Oliveira
Summary: The study demonstrated that Telmisartan (TELM) inhibited osteogenic differentiation and mineralization of SHR BMSC, favoring an adipogenic phenotype due to Pparg upregulation.
EUROPEAN JOURNAL OF PHARMACOLOGY
(2021)
Review
Pharmacology & Pharmacy
Vishal Jhanji, Isabelle Billig, Gary Hin-Fai Yam
Summary: Corneal opacification is the fourth most common cause of blindness globally. The standard treatment for serious corneal scarring is corneal transplantation, but there are limitations such as long-term graft survival and the lifelong use of immunosuppressants. Research is focused on finding new technologies to modulate wound healing and prevent corneal opacification.
FRONTIERS IN PHARMACOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Martin Bablok, Morris Gellisch, Beate Brand-Saberi, Gabriela Morosan-Puopolo
Summary: Understanding the complex processes of fetal wound healing and skin regeneration can improve fetal surgery, but local glucocorticoid administration may decelerate the healing of skin incisional wounds, resulting in morphological changes.
Article
Chemistry, Multidisciplinary
Ihsan Safwan Kamarazaman, Nor Azah Mohamad Ali, Fauziah Abdullah, Nazrin Che Saad, Aida Azlina Ali, Salfarina Ramli, Pornchai Rojsitthisak, Hasseri Halim
Summary: This study investigated the wound healing and antioxidant properties of ethanolic extract of Baeckea frutescens leaves. The extract was found to promote cell proliferation and migration, and exhibited strong antioxidant activity. Phytochemical analysis revealed the presence of saponins, flavonoids, tannins, and steroids in the extract, which may contribute to its antioxidant and wound healing properties.
ARABIAN JOURNAL OF CHEMISTRY
(2022)
Article
Chemistry, Multidisciplinary
Ly Thi Huong Nguyen, Sang-Hyun Ahn, Min-Jin Choi, In-Jun Yang, Heung-Mook Shin
Summary: Puerarin has been shown to promote wound healing by accelerating wound closure rate, increasing keratinocyte proliferation and migration, and enhancing capillary density and collagen fiber levels at the wound sites. Additionally, it activates the ERK and Akt signaling pathways to facilitate keratinocyte function. These findings suggest that puerarin could be a potential treatment for delayed and impaired wound healing caused by dexamethasone.
APPLIED SCIENCES-BASEL
(2021)
Article
Food Science & Technology
Afraa Maiza, Tesnime Ghazouani, Franks Kamgang Nzekoue, Giovanni Caprioli, Dennis Fiorini, Sauro Vittori, Beatrice Francucci, Gabriella Marucci, Michela Buccioni, Sami Fattouch
Summary: Ayurvedic literature has proven the healing properties of ghee, which can be used for wound healing. This study compares different types of Tunisian ghee and finds that ghee with high antioxidant capacity and rich in vitamins A and E can improve fibroblast migration.
INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Paulina Escandon, Angela Liu, Sarah E. Nicholas, Asher Khan, Kamran M. Riaz, Dimitrios Karamichos
Summary: This study explores the role of salivary exosomes in corneal wound healing for the first time. The results show that stimulation with salivary exosomes can slow down wound closure in healthy individuals and diabetic patients, and it affects the expression of certain proteins. However, it is still unclear whether salivary exosomes can be used in clinical settings to restore corneal defects.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Lukasz Szymanski, Slawomir Lewicki, Tomasz Markiewicz, Szczepan Cierniak, Jean-Pierre Tassan, Jacek Z. Kubiak
Summary: Skin wounds are a significant problem for the healthcare system, impacting clinical outcomes, patient quality of life, and financial costs. Faster wound healing could benefit both patients and the healthcare system in terms of clinical, economic, and social aspects. This study aimed to assess the potential of MELK silencing in accelerating wound healing. MELK siRNA treatment was found to lead to faster wound closure and increased collagen deposition.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Endocrinology & Metabolism
Ya Li, Jing Li, Can Zhao, Lingling Yang, Xia Qi, Xiaochuan Wang, Qingjun Zhou, Weiyun Shi
Summary: Hyperglycemia reduces NAD(+) biosynthesis in the cornea, leading to impaired epithelial wound healing in diabetic mice and patients. Replenishment of NAD(+) and its precursors can facilitate corneal wound healing and nerve regeneration in diabetic conditions, suggesting a novel therapeutic strategy for diabetic corneal complications.
METABOLISM-CLINICAL AND EXPERIMENTAL
(2021)
Article
Endocrinology & Metabolism
Matthew L. Potter, William D. Hill, Carlos M. Isales, Mark W. Hamrick, Sadanand Fulzele
Summary: miRNAs and cellular senescence have been implicated in driving age-related diseases, particularly in mesenchymal stem cells. Differential expression of key miRNAs in MSCs and other musculoskeletal cells during senescence and aging, regulation of miRNAs via SASP cytokines, and targeting of components of cell cycle arrest pathways by miRNAs have been identified. Identifying potential miRNA targets may have implications for regenerative medicine applications in age-related musculoskeletal diseases.
Article
Biochemistry & Molecular Biology
Ibrahim Yusufu, Kehong Ding, Kathryn Smith, Umesh D. Wankhade, Bikash Sahay, G. Taylor Patterson, Rafal Pacholczyk, Satish Adusumilli, Mark W. Hamrick, William D. Hill, Carlos M. Isales, Sadanand Fulzele
Summary: This study investigated the impact of varied tryptophan diets on the gut microbiome and inflammatory responses in aged mice models, finding that tryptophan-deficient diets led to changes in gut bacterial abundance and increased levels of inflammatory cytokines IL-6, IL-17A, and IL-1a.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Endocrinology & Metabolism
Nada H. Eisa, Periyasamy T. Sudharsan, Sergio Mas Herrero, Samuel A. Herberg, Brian F. Volkman, Alexandra Aguilar-Perez, Dmitry Kondrikov, Ahmed M. Elmansi, Charles Reitman, Xingming Shi, Sadanand Fulzele, Meghan E. McGee-Lawrence, Carlos M. Isales, Mark W. Hamrick, Maribeth H. Johnson, Jie Chen, William D. Hill
Summary: Age-associated osteoporosis is believed to be related to the disruption of osteogenic stem cell populations. MicroRNAs have been found to play a potential role in regulating BMSCs and the BM microenvironment with aging, with miR-29b-1-5p identified as a critical miRNA in controlling BMSC osteogenesis.
Article
Endocrinology & Metabolism
Jessica L. Pierce, Anuj K. Sharma, Rachel L. Roberts, Kanglun Yu, Debra L. Irsik, Vivek Choudhary, Jennifer S. Dorn, Husam Bensreti, Reginald D. Benson, Helen Kaiser, Andrew Khayrullin, Colleen Davis, Chase J. Wehrle, Maribeth H. Johnson, Wendy B. Bollag, Mark W. Hamrick, Xingming Shi, Carlos M. Isales, Meghan E. McGee-Lawrence
Summary: Deficiency in glucocorticoid receptor (GR) in aging-associated osteoporosis leads to low bone mass and bone marrow adipose tissue accumulation, as well as alterations in muscle mass, metabolic rate, and blood pressure. The study suggests a role for GR in facilitating healthy bone maintenance with aging in females and highlights the importance of bone as an endocrine organ.
JOURNAL OF BONE AND MINERAL RESEARCH
(2022)
Article
Physiology
Ling Ruan, Bharati Mendhe, Emily Parker, Andrew Kent, Carlos M. Isales, William D. Hill, Meghan McGee-Lawrence, Sadanand Fulzele, Mark W. Hamrick
Summary: This study discovered that the expression of lncRNA MALAT1 decreases with age in skeletal muscle, accompanied by an increase in miR-34a-5p expression. In vitro experiments showed that silencing MALAT1 increases miR-34a expression. Additionally, oxidative stress reduces MALAT1 expression, but this effect can be attenuated by p53 siRNA. Furthermore, MALAT1 silencing significantly increases TGF-beta 1 expression in C2C12 cells.
FRONTIERS IN PHYSIOLOGY
(2022)
Article
Medicine, Research & Experimental
Emily J. Gallagher, Don C. Rockey, Christopher D. Kontos, Jatin M. Vyas, Lawrence F. Brass, Patrick J. Hu, Carlos M. Isales, Olujimi A. Ajijola, W. Kimryn Rathmell, Paul R. Conlin, Robert A. Baiocchi, Barbara Kazmierczak, Myles H. Akabas, Christopher S. Williams
Summary: Postgraduate physician-scientist training programs (PSTPs) are instrumental in enhancing the development of physician-scientist trainees. A survey of PSTP directors in the US revealed that having first-author publications and a strong recommendation from the thesis advisor were considered crucial components of a successful PSTP application. This highlights the importance of scientific productivity and commitment to research in the selection process, as opposed to traditional measures of medical school performance.
Article
Endocrinology & Metabolism
Yanyan Xu, Shaoyong Su, William McCall, Carlos Isales, Harold Snieder, Xiaoling Wang
Summary: Circadian rhythm disturbance is significantly associated with impaired glucose homeostasis in a general population of adults without diabetes, suggesting that circadian dysfunction may contribute to early disease pathogenesis. The study analyzed data from 2760 adults without diabetes from the NHANES 2011-2014, showing that increased circadian rhythmicity was associated with lower 2-hour glucose levels and decreased odds of IGT, while increased rhythm fragmentation was associated with higher 2-hour glucose levels and increased odds of IGT.
BMJ OPEN DIABETES RESEARCH & CARE
(2022)
Review
Geriatrics & Gerontology
Andrew Murphy, Sagar Vyavahare, Sandeep Kumar, Tae Jin Lee, Ashok Sharma, Satish Adusumilli, Mark Hamrick, Carlos M. Isales, Sadanand Fulzele
Summary: This study summarizes the research on age-associated diseases and the efforts to extend a healthy lifespan. It discusses the positive effects of dietary protocols such as caloric restriction and protein restriction on human longevity. The study also analyzes the impacts of these protocols on bone and muscle biology, identifying new genes and signaling pathways involved in musculoskeletal tissue.
Article
Geriatrics & Gerontology
Dhara Patel, Tae Jin Lee, Sandeep Kumar, Sagar Vyavahare, Alison Worth, William D. Hill, Mark Hamrick, Carlos M. Isales, Rahul S. Shinde, Sadanand Fulzele
Summary: Understanding the pathophysiology of age-related diseases is crucial as the elderly population grows. Metabolic changes play an important role in age-related degeneration, affecting musculoskeletal health. Gender-specific differences in metabolic signaling pathways may contribute to the manifestation of musculoskeletal diseases in males and females.
Article
Endocrinology & Metabolism
Yun Su, Xing Chen, Hongyan Zhou, Sean Shaw, Jie Chen, Carlos M. Isales, Jing Zhao, Xingming Shi
Summary: Glucocorticoids are potent drugs with anti-inflammatory and immunosuppressive effects, but their clinical use is limited due to severe side effects. The molecular mechanisms underlying glucocorticoid-induced bone loss are unclear. In this study, RNA sequencing analysis identified Xist as a glucocorticoid-regulated gene involved in glucocorticoid-induced osteoporosis.
FRONTIERS IN ENDOCRINOLOGY
(2022)
Article
Endocrinology & Metabolism
Alexis M. Stranahan, De-Huang Guo, Masaki Yamamoto, Caterina M. Hernandez, Hesam Khodadadi, Babak Baban, Wenbo Zhi, Yun Lei, Xinyun Lu, Kehong Ding, Carlos M. Isales
Summary: Preferential energy storage in subcutaneous adipose tissue (SAT) provides protection against obesity-induced pathophysiology in females. Differences in adipose tissue distribution before obesity onset determine susceptibility to inflammation in males and females, independent of sex hormones.
Article
Biology
P. Robinson Muller, Tae Jin Lee, Wenbo Zhi, Sandeep Kumar, Sagar Vyavahare, Ashok Sharma, Vikas Kumar, Carlos M. Isales, Monte Hunter, Sadanand Fulzele
Summary: Osteoarthritis is a common degenerative joint disease that primarily affects females. Research has shown that the protein composition of synovial fluid in female osteoarthritis patients differs, which may provide insights into gender-specific pathophysiology.
Article
Endocrinology & Metabolism
Laura Carbone, Petra Buzkova, Howard A. Fink, John A. Robbins, Joshua Barzilay, Rachel E. Elam, Carlos Isales
Summary: Amino acids are important for skeletal health, and the relationship between tryptophan and its metabolites with hip fractures and mortality has been examined. Higher serum levels of tryptophan were associated with lower risk of hip fractures, but no significant associations were found with mortality or frailty status. Further randomized clinical trials are needed to investigate the potential benefits of increased tryptophan intake for osteoporosis.
Article
Neurosciences
Vivek Choudhary, Etsubdenk Ajebo, Rawipan Uaratanawong, Shinjini C. Spaulding, Sarah Hossack, Xunsheng Chen, Jianrui Xu, Mrunal Choudhary, Debra L. Irsik, Carlos M. Isales, Wendy B. Bollag
Summary: A study found that the lack of the IDO1 gene does not have a significant effect on the immune response and skin lesions in a psoriasis model.
INTERNATIONAL JOURNAL OF TRYPTOPHAN RESEARCH
(2022)
Article
Geriatrics & Gerontology
Nicholas Cantu, Sagar Vyavahare, Sandeep Kumar, Jie Chen, Ravindra Kolhe, Carlos M. Isales, Mark Hamrick, Sadanand Fulzele
Summary: The study investigated the molecular mechanisms underlying muscle loss in COVID-19 patients, finding that cytokines, glucocorticoids, and antiviral drugs may synergistically contribute to this outcome.