Article
Hematology
Brittany Balint, Jan M. Federspiel, Tanja Schwab, Tristan Ehrlich, Frank Ramsthaler, Hans-Joachim Schaefers
Summary: Patients with aortic regurgitation have more severe medial degeneration in the ascending aortic wall compared to those with aortic stenosis or normal valves. This pathological remodeling includes mucoid extracellular matrix accumulation, elastin loss, elastin fragmentation, decreased expression of fibrillin and collagen. Additionally, decreased eNOS expression and increased subendothelial apoptosis were observed in the aortas with aortic regurgitation.
ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Anna Di Vito, Annalidia Donato, Ivan Presta, Teresa Mancuso, Francesco Saverio Brunetti, Pasquale Mastroroberto, Andrea Amorosi, Natalia Malara, Giuseppe Donato
Summary: Calcific Aortic Valve Disease (CAVD) is the most common valvular heart disease in the ageing population, which is strongly correlated with active and degenerative processes. The progression of the disease is closely related to the cellular and matrix alterations within the aortic valve.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Cardiac & Cardiovascular Systems
Cassandra L. L. Clift, Janet Saunders, Richard R. R. Drake, Peggi M. M. Angel
Summary: This article reviews the impact of extracellular matrix dysregulation on valve function in pediatric congenital aortic valve stenosis (pCAVS). It discusses the mechanisms of translational and post-translational dysregulation of ECM and explores contributing factors to this dysregulation. The article also draws parallels between ECM post-translational modifications in pCAVS and other fibrotic diseases. Additionally, it examines current treatment options in pediatrics and highlights the advancements in proteomics that may lead to potential therapeutic strategies for pCAVS.
FRONTIERS IN CARDIOVASCULAR MEDICINE
(2022)
Article
Biochemistry & Molecular Biology
Christian M. Beusch, Oscar E. Simonson, Johan O. Wedin, Pierre Sabatier, Ulrika Felldin, Sandeep Kadekar, Cecilia Osterholm, Akos Vegvari, Roman A. Zubarev, Karin Fromell, Bo Nilson, Stefan James, Elisabeth Stahle, Karl-Henrik Grinnemo, Sergey Rodin
Summary: By comparing the extracellular matrix proteins in valve tissues from patients with isolated aortic valve degeneration, the study identified differences in the molecular cues and aetiologies between patients with tricuspid and bicuspid aortic valves, suggesting the need for different treatments and providing insights into the molecular basis of the condition.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2023)
Article
Cardiac & Cardiovascular Systems
Ana Rubina Perestrelo, Ana Catarina Silva, Jorge Oliver-De La Cruz, Fabiana Martino, Vladimir Horvath, Guido Caluori, Ondrej Polansky, Vladimir Vinarsky, Giulia Azzato, Giuseppe de Marco, Vita Zampachova, Petr Skladal, Stefania Pagliari, Alberto Rainer, Perpetua Pinto-do-O, Alessio Caravella, Kamila Koci, Diana S. Nascimento, Giancarlo Forte
Summary: The study reveals that the remodeling of cardiac extracellular matrix (ECM) during heart failure (HF) leads to cardiac fibroblast activation and focal adhesion protein expression through hyperactivated YAP signaling, impacting cell homing.
CIRCULATION RESEARCH
(2021)
Article
Medicine, Research & Experimental
Isabel N. Schellinger, Markus Wagenhaeuser, Giriprakash Chodisetti, Karin Mattern, Angelika Dannert, Anne Petzold, Joanna Jakubizka-Smorag, Fabian Emrich, Josephina Haunschild, Andreas Schuster, Elisabeth Schwob, Kei Schulz, Lars Maegdefessel, Joshua M. Spin, Michael Stumvoll, Gerd Hasenfuss, Philip S. Tsao, Uwe Raaz
Summary: Patients with type 2 diabetes are at increased risk of cardiovascular morbidity and mortality, often linked to accelerated arterial stiffening. This study identifies miR-29b as a key regulator of diabetic aortic remodeling and stiffening, with its downregulation leading to fibrosis and elastin breakdown in the aorta. Targeting miR-29b may offer a potential therapeutic strategy for mitigating cardiovascular risks in type 2 diabetes.
MOLECULAR THERAPY-NUCLEIC ACIDS
(2021)
Article
Biochemistry & Molecular Biology
Xiaojing Zhou, Abdullah Md. Sheikh, Ken-ichi Matsumoto, Shingo Mitaki, Abu Zaffar Shibly, Yuchi Zhang, A. Garu, Shozo Yano, Atsushi Nagai
Summary: Through proteomic analysis of urine exosomes in AD model mice, we identified proteins related to lipid metabolism and A beta metabolism in the early stages of AD, providing new insights into the underlying pathological mechanism of early AD.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Environmental Sciences
Yue-Him Wong, Yu Zhang, Janice C. Y. Lun, Jian-Wen Qiu
Summary: By comparing the proteome of normal and GA affected tissues in the brain coral, it was found that 117 differentially expressed proteins were identified, some of which might be related to GA. The study provided novel insights into the molecular pathology of coral GA, paving the way for determining the causative agent(s) of this coral disease.
MARINE POLLUTION BULLETIN
(2021)
Article
Cardiac & Cardiovascular Systems
Jan M. Federspiel, Philipp A. Schnabel, Thomas Tschernig, Brittany Balint, Tanja Schwab, Matthias W. Laschke, Hans-Joachim Schaefers
Summary: The study revealed more apoptosis in the aortic walls of patients with unicuspid aortic valve (UAV), while more severe mucoid extracellular matrix accumulation (MEMA) was found in aortic aneurysms of patients with tricuspid aortic valve (TAV) compared to UAV. This suggests that UAV-associated aortic wall changes and resulting aneurysm may be less aggressive than those with TAV.
EUROPEAN JOURNAL OF CARDIO-THORACIC SURGERY
(2021)
Article
Cardiac & Cardiovascular Systems
Kaitlyn Thatcher, Carol R. Mattern, Daniel Chaparro, Veronica Goveas, Michael R. Mcdermott, Jessica Fulton, Joshua D. Hutcheson, Brian R. Hoffmann, Joy Lincoln
Summary: The organization of extracellular matrix (ECM) components is crucial for proper heart valve structure and function. Mutations in ECM genes can lead to connective tissue disorders and heart valve dysfunction. A study on mice with a specific mutation was conducted to understand the pathobiology of aortic valve disease and identified early abnormalities in ECM homeostasis that can be targeted for early treatment to prevent late-stage surgical intervention.
JOURNAL OF CARDIOVASCULAR DEVELOPMENT AND DISEASE
(2023)
Article
Cardiac & Cardiovascular Systems
Manon Meerman, Rob Driessen, Nicole C. A. van Engeland, Irith Bergsma, Jacco L. G. Steenhuijsen, David Kozono, Elena Aikawa, Jesper Hjortnaes, Carlijn V. C. Bouten
Summary: The study demonstrates that radiation exposure enhances the calcific response in VICs, contributing to the development of valvular disease. High radiation exposure induces the differentiation of VICs into terminally differentiated giant-cell fibroblasts. Further research is needed to uncover the underlying mechanisms of radiation-induced valvular changes.
FRONTIERS IN CARDIOVASCULAR MEDICINE
(2021)
Review
Cardiac & Cardiovascular Systems
Nikolaos Anousakis-Vlachochristou, Dimitra Athanasiadou, Karina M. M. Carneiro, Konstantinos Toutouzas
Summary: This study reviews the alterations of structural native extracellular matrix proteins involved in the development of calcific aortic valve stenosis (CAVS) and highlights its link to deregulated biomechanical function.
JACC-BASIC TO TRANSLATIONAL SCIENCE
(2023)
Article
Cell Biology
Theresa Holst, Johannes Petersen, Sabine Ameling, Lisa Mueller, Torsten Christ, Naomi Gedeon, Thomas Eschenhagen, Hermann Reichenspurner, Elke Hammer, Evaldas Girdauskas
Summary: This study identified specific left ventricular (LV) myocardial protein signatures that contribute to the differential disease progression in patients with chronic aortic regurgitation (AR) and aortic stenosis (AS) after aortic valve (AV) surgery.
Review
Biotechnology & Applied Microbiology
Victor Alfonso Solarte David, Viviana Raquel Guiza-Argueello, Martha L. Arango-Rodriguez, Claudia L. Sossa, Silvia M. Becerra-Bayona
Summary: The absence or damage of tissue is a major cause of diseases, and novel therapeutic alternatives aim to recover lost functions through tissue regeneration. Chronic cutaneous lesions are common wounds that require regenerative medicine and tissue engineering interventions to develop bioactive medical products for proper healing and prevention of complications. The coordination of cells, extracellular matrix (ECM), and biomolecules is crucial in tissue repair and regeneration, with ECM acting as a biological platform for their interactions. Tissue engineering utilizes synthetic polymers and decellularized tissues to mimic native ECM and provide necessary functionalities for cell differentiation.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Marina Marozzi, Arianna Parnigoni, Aide Negri, Manuela Viola, Davide Vigetti, Alberto Passi, Evgenia Karousou, Federica Rizzi
Summary: Cancer is a complex pathology characterized by uncontrolled cell proliferation and decreased apoptosis, often in an inflammatory environment. Changes in the extracellular matrix (ECM) and signaling pathways play crucial roles in tumor growth and metastasis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Cardiac & Cardiovascular Systems
Elena Rodriguez-Sanchez, Jose Alberto Navarro-Garcia, Jennifer Aceves-Ripoll, Laura Gonzalez-Lafuente, Montserrat Baldan-Martin, Fernando de la Cuesta, Gloria Alvarez-Llamas, Maria G. Barderas, Julian Segura, Luis M. Ruilope, Gema Ruiz-Hurtado
Summary: This study aimed to investigate whether arterial stiffness assessed with active matrix metalloproteinase (MMP)-9, pulse pressure (PP), and pulse wave velocity can predict the response to spironolactone in patients with resistant hypertension (RH). The results showed that plasma active MMP-9 was significantly higher in patients with RH and correlated with systolic blood pressure and pulse pressure. Active MMP-9, in combination with 24-hour pulse pressure and pulse wave velocity, improved the prediction of the response to spironolactone. Furthermore, the plasma of patients with uncontrolled RH induced MMP-9 expression pathway. Therefore, active MMP-9 can be a useful biomarker in identifying patients with RH who will not respond to spironolactone, and combining MMP-9 activity with classical arterial stiffness parameters can improve the prediction of the clinical response to spironolactone.
EUROPEAN HEART JOURNAL-CARDIOVASCULAR PHARMACOTHERAPY
(2022)
Article
Immunology
Silvia Lozano-Edo, Ignacio Sanchez-Lazaro, Manuel Portoles, Esther Rosello-Lleti, Estefania Tarazon, Miguel Angel Arnau-Vives, Meryem Ezzitouny, Raquel Lopez-Vilella, Luis Almenar-Bonet, Luis Martinez-Dolz
Summary: This study suggests that assessing plasma levels of SERCA2a may improve risk prediction for primary graft dysfunction (PGD) and could serve as a new noninvasive biomarker in patients undergoing heart transplantation (HT).
Article
Cardiac & Cardiovascular Systems
Lorena Perez-Carrillo, Ignacio Sanchez-Lazaro, Juan Carlos Trivino, Sandra Feijoo-Bandin, Francisca Lago, Jose Ramon Gonzalez-Juanatey, Luis Martinez-Dolz, Manuel Portoles, Estefania Tarazon, Esther Rosello-Lleti
Summary: Noninvasive approaches for early diagnosis of acute cellular rejection (ACR) in cardiac transplantation are important. This study identified serum miR-144-3p as a potential biomarker for ACR, with excellent diagnostic accuracy and predictive value for the presence of rejection episodes.
JOURNAL OF HEART AND LUNG TRANSPLANTATION
(2022)
Article
Pharmacology & Pharmacy
Alana Aragon-Herrera, Manuel Otero-Santiago, Laura Anido-Varela, Sandra Morana-Fernandez, Manuel Campos-Toimil, Tomas Garcia-Caballero, Luis Barral, Estefania Tarazon, Esther Rosello-Lleti, Manuel Portoles, Oreste Gualillo, Isabel Moscoso, Ricardo Lage, Jose Ramon Gonzalez-Juanatey, Sandra Feijoo-Bandin, Francisca Lago
Summary: This study found that empagliflozin can alter the hepatic lipid metabolism profile, showing a protective profile. Additionally, empagliflozin also decreases the levels of inflammatory markers in the liver. These results provide new evidence regarding the molecular pathways through which empagliflozin could exert hepatoprotective effects in patients with type 2 diabetes.
FRONTIERS IN PHARMACOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Ainara Gonzalez-Moro, Ines Valencia, Licia Shamoon, Carlos Felix Sanchez-Ferrer, Concepcion Peiro, Fernando de la Cuesta
Summary: Despite advancements in medicine, mortality from cardiovascular diseases continues to increase due to the pandemic prevalence of obesity and diabetes, as well as the aging global population. The discovery of the potential of anti-inflammatory therapies targeted to IL-1 beta, particularly through the use of the NLRP3 inflammasome blockade, is changing the therapeutic management of cardiovascular diseases. This review discusses the mechanisms by which the NLRP3 inflammasome contributes to vascular disease, with a focus on aging and chronic low-grade inflammation.
Article
Health Care Sciences & Services
Estefania Tarazon, Lorena Perez-Carrillo, Manuel Portoles, Esther Rosello-Lleti
Summary: This study focuses on analyzing new ultrastructural findings in cardiac biopsy rejection through the observation of perinuclear clustering of mitochondria, which could complement and improve the diagnosis of rejection.
JOURNAL OF PERSONALIZED MEDICINE
(2022)
Review
Biochemistry & Molecular Biology
Sandra Feijoo-Bandin, Alana Aragon-Herrera, Manuel Otero-Santiago, Laura Anido-Varela, Sandra Morana-Fernandez, Estefania Tarazon, Esther Rosello-Lleti, Manuel Portoles, Oreste Gualillo, Jose Ramon Gonzalez-Juanatey, Francisca Lago
Summary: Sodium-glucose co-transporter 2 inhibitors, known as gliflozins, not only regulate glucose homeostasis and show cardio-renal protective effects in patients with heart failure, but also have anti-inflammatory properties, which may be beneficial for treating other diseases associated with inflammation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Estefania Tarazon, Lorena Perez-Carrillo, Isaac Gimenez-Escamilla, Maria Garcia-Manzanares, Luis Martinez-Dolz, Manuel Portoles, Esther Rosello-Lleti
Summary: We conducted a study on the DNA methylation process in human cardiac tissue of ischemic cardiomyopathy patients and found dysregulation in this process. We observed genome-wide hypomethylation along with overexpression of genes involved in methyl group elimination and underexpression of molecules involved in methylation maintenance. Despite the upregulation of DNMT3B and downregulation of miR-133a-3p, we identified relevant alterations that counteracted the observed upregulation. We also found changes in molecules regulating Dnmts activity. Our findings reveal genome-wide hypomethylation and dysregulation in the DNA methylation machinery in ischemic cardiomyopathy.
Article
Health Care Sciences & Services
Silvia Lozano-Edo, Esther Rosello-Lleti, Ignacio Sanchez-Lazaro, Estefania Tarazon, Manuel Portoles, Maryem Ezzitouny, Raquel Lopez-Vilella, Miguel Angel Arnau, Luis Almenar, Luis Martinez-Dolz
Summary: This study found that serum RANGAP1 levels can serve as a non-invasive biomarker for predicting the risk of ACR during the first year after heart transplantation.
JOURNAL OF PERSONALIZED MEDICINE
(2022)
Editorial Material
Biochemistry & Molecular Biology
Maria G. G. Barderas, Fernando de la Cuesta
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Medical Laboratory Technology
Nerea Corbacho-Alonso, Tamara Sastre-Oliva, Luis F. Lopez-Almodovar, Jorge Solis, Luis R. Padial, Teresa Tejerina, Montserrat Carrascal, Laura Mourino-Alvarez, Maria G. Barderas
Summary: Diabetes mellitus (DM) increases the risk of severe calcific aortic stenosis (CAS), but the relationship between these two diseases and the impact of DM on CAS progression is unclear. In this study, we analyzed calcified and noncalcified valve tissue from patients with severe CAS, with or without DM, using a proteomic strategy. We found that DM induces changes in the proteome of aortic valves, affecting valve calcification. This finding provides valuable insights into the pathogenesis of CAS and the personalized treatment of patients with both DM and CAS.
TRANSLATIONAL RESEARCH
(2023)
Article
Biochemistry & Molecular Biology
Nerea Corbacho-Alonso, Elena Rodriguez-Sanchez, Tamara Sastre-Oliva, Elisa Mercado-Garcia, Ines Perales-Sanchez, Cristina Juarez-Alia, Luis F. Lopez-Almodovar, Luis R. Padial, Teresa Tejerina, Laura Mourino-Alvarez, Gema Ruiz-Hurtado, Maria G. Barderas
Summary: Calcific aortic stenosis (CAS) and type 2 diabetes mellitus (T2DM) often coexist and are related, accompanied by common comorbidities. Oxidative stress plays a role in CAS and vascular complications in T2DM. Metformin, a medication for T2DM, may reduce oxidative stress. This study evaluated the oxidative status in plasma from CAS patients, with or without T2DM and metformin treatment, suggesting that reducing oxidative stress or enhancing antioxidant capacity could be a strategy for managing CAS.
Article
Chemistry, Medicinal
Nuria Vilaboa, Juan Antonio Lopez, Marco de Mesa, Clara Escudero-Duch, Natalie Winfield, Melanie Bayford, Richard Voellmy
Summary: Exposure to celastrol, withaferin A, and synthetic compounds caused denaturation of luciferase reporter protein in cancer cells, leading to proteostasis disruption. Proteomic analysis revealed covalent modification of numerous cellular proteins targeted by the compounds. Important fractions of these proteins unfolded and formed aggregates. Multiple myeloma cells were particularly sensitive to these compounds. Development of a new proteostasis-disrupting therapy for multiple myeloma is suggested.
Article
Cell Biology
Susana Aguilar, Paula Garcia-Olloqui, Lidia Amigo-Moran, Jose Luis Toran, Juan Antonio Lopez, Guillermo Albericio, Gloria Abizanda, Diego Herrero, Africa Vales, Saray Rodriguez-Diaz, Marina Higuera, Ruben Garcia-Martin, Jesus Vazquez, Carmen Mora, Gloria Gonzalez-Aseguinolaza, Felipe Prosper, Beatriz Pelacho, Antonio Bernad
Summary: Oxidative stress-induced myocardial apoptosis and necrosis play a critical role in ischemic infarction. This study identified and validated miR-935 as a highly differentially expressed miRNA in exosomes derived from human cardiac progenitor cells (CPC). Reduction of miR-935 expression promotes oxidative stress-associated apoptosis. Together with other exosomal miRNA members, miR-935 may counteract oxidative stress-related apoptosis in the CPC microenvironment.