Article
Electrochemistry
Fakhriy Muhammad, Gamze Dik, Seda Kolak, Kubra Karadas Gedik, Busra Bakar, Ahmet Ulu, Burhan Ates
Summary: In this study, PCL/PEI nanofiber membranes were prepared and used for the immobilization of uricase, allowing for a simple, rapid, and sensitive detection method for UA in electrochemical applications.
ELECTROCHIMICA ACTA
(2023)
Article
Chemistry, Analytical
Baha Ondes, Cagdas Sunna, Ulviye Kilimci, Murat Uygun, Deniz Aktas Uygun
Summary: In this study, enzyme-modified boron nitride nanosheets were developed as a catalytic two-dimension material for sensor applications. The developed enzyme-based biosensor exhibited wide linear detection range, low limit of detection and quantitation values, good reproducibility, reusability, high selectivity, sensitivity, low Km value, and excellent shelf-life properties. It has also successfully identified uric acid in human serum with high recovery rate, promising rapid and high sensitivity applications in real serum.
MICROCHEMICAL JOURNAL
(2023)
Article
Chemistry, Analytical
David Majer, Matjaz Finsgar
Summary: This study demonstrated the analysis of epinephrine and uric acid using a novel method, validating the accuracy and effectiveness through data verification and comparison between OLS and WLR methods.
Article
Chemistry, Analytical
Sofiia Tvorynska, Jiri Barek, Bohdan Josypcuk
Summary: This study presents a novel strategy for uric acid determination using highly stable flow biosensors and a four-electron reduction of consumed oxygen. The design of the biosensors allows for spatial separation of detection and biorecognition parts, resulting in accurate measurements of uric acid concentration. The immobilization strategy of enzymes significantly affects the performance of the biosensors, with physical adsorption of uricase to glassy carbon showing good sensitivity, stability, and repeatability.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Article
Electrochemistry
Jessica Rocha Camargo, Tiago Almeida Silva, Gustavo A. Rivas, Bruno C. Janegitz
Summary: This paper presents a novel water-based conductive ink and successfully applies it in the screen-printing of disposable electrodes. The electrode shows good linear relationship and low detection limit in the quantification of uric acid and catechol. The new electrode demonstrates outstanding analytical characteristics and has the potential to be a disposable, low-cost, and environmentally friendly platform for electrochemical sensing and biosensing purposes.
ELECTROCHIMICA ACTA
(2022)
Article
Electrochemistry
Wulan Tri Wahyuni, Budi Riza Putra, Rudi Heryanto, Eti Rohaeti, Dede Heri Yuli Yanto, Achmad Fauzi
Summary: This work presents a simple technique for homemade SPCE fabrication and its application in uric acid detection. The homemade SPCE showed better performance in electrochemical characterization and uric acid measurements compared to commercially available SPCE, with higher signal, lower detection limit, good reproducibility, and sensitivity. It also demonstrated good selectivity in detecting uric acid in human urine, indicating its potential for further development as an electrochemical sensor.
INTERNATIONAL JOURNAL OF ELECTROCHEMICAL SCIENCE
(2021)
Review
Biotechnology & Applied Microbiology
Gang Liang, Zhaoying He, Jianhui Zhen, Hao Tian, Lianfeng Ai, Ligang Pan, Wenwen Gong
Summary: Periodically excessive use of pesticides in agricultural production has led to ecotoxicity and environmental pollution. Biosensing technique is a complementary tool to classical chromatographic methods for rapid, in situ analysis. Screen-printed electrode (SPE) is a widely used method for pesticides detection due to its low cost, easy integration, versatility, and portability. This review provides an overview of the screen-printing technique and SPE, including their definition, preparation procedure, advantages, and discusses the utilization of SPE in detecting pesticides using various bioreceptors and nanomaterials. The sensing principles, analytical performance, advantages, and shortcomings of SPE-based sensors are summarized, and the current challenges and future directions for SPE and SPE-based electrochemical sensors for pollutants are outlined.
ENVIRONMENTAL TECHNOLOGY & INNOVATION
(2022)
Article
Chemistry, Applied
A. Smart, A. Crew, O. Doran, J. P. Hart
Summary: This paper presents the development of a novel trilinolein biosensor using lipase and lipoxygenase immobilized on a cobalt phthalocyanine screen-printed carbon electrode. The biosensor demonstrates selective response to polyunsaturated fatty acid (PUFA) triglycerides, distinguishing them from monounsaturated (MUFA) and saturated fatty acid (SFA) triglycerides. The biosensor shows a linear range of 0.2-10 μM and a detection limit of 45.5 nM. It has been successfully applied to measure trilinolein in a pharmaceutical food supplement with a recovery rate of 86% and a coefficient of variation of 5.05%. This simple and cost-effective LOX-lipase biosensor shows great potential for various applications, including food and clinical samples.
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
(2023)
Article
Chemistry, Analytical
Mustafa Sen, Merve Oguz, Ipek Avci
Summary: A non-toxic, flexible, low-cost, and disposable multiwalled carbon nanotube (MWCNT)-based screen-printed electrode (SPE) was developed for non-invasive health monitoring applications. The electrode demonstrated excellent electrochemical behavior and is expected to contribute to wearable sensor technology.
Article
Chemistry, Multidisciplinary
Siti Fatimah Nur Abdul Aziz, Abu Bakar Salleh, Siti Efliza Ashari, Yahaya M. Normi, Nor Azah Yusof, Shahrul Ainliah Alang Ahmad
Summary: This study developed a nanozyme-based electrochemical biosensor using mp20@ZIF-8/rGO/SPCE as a bioreceptor for uric acid detection, showing high selectivity and practicality in monitoring uric acid in biological samples.
Article
Multidisciplinary Sciences
Michaela Pavlickova, Lenka Lorencova, Michal Hatala, Miroslav Kovac, Jan Tkac, Pavol Gemeiner
Summary: Molybdenum disulfide (MoS2) screen-printed working electrodes were developed for dopamine (DA) electrochemical sensing. The MoS2 ink was prepared with different concentrations and sizes of MoS2 particles and an ethylcellulose binder. The screen-printed MoS2 electrodes showed a linear detection range of dopamine from 1 mu M to 300 mu M with a sensitivity of 5.00 x 10(-8) A mu M-1. The low-cost and rapid preparation of MoS2 ink and screen-printed electrodes demonstrated its potential for dopamine detection.
SCIENTIFIC REPORTS
(2022)
Review
Chemistry, Analytical
Preeyaporn Reanpang, Pijika Mool-am-kha, Jantima Upan, Jaroon Jakmunee
Summary: A simple, rapid, and cost-effective flow injection amperometric sensor was developed for sensitive determination of uric acid, utilizing a new combination of carbon black and graphene oxide modified screen-printed carbon electrode. The modified electrode showed enhanced sensitivity, stability, and automation for uric acid detection. The system achieved good selectivity, repeatability, and stability, with a detection limit of 0.01 µM and a throughput of 25 injections per hour.
Article
Engineering, Electrical & Electronic
Mohammad Reza Baezzat, Nahid Tavakkoli, Hassan Zamani
Summary: This study developed a novel electrochemical sensor based on MoS2 nanosheets modified graphite screen-printed electrode for sensitive detection of isoproterenol in the presence of other compounds. The sensor showed linear response to isoproterenol concentration under optimized conditions with a low detection limit.
JOURNAL OF MATERIALS SCIENCE-MATERIALS IN ELECTRONICS
(2022)
Article
Biophysics
Xiuguang Xing, Bangben Yao, Qian Wu, Ru Zhang, Li Yao, Jianguo Xu, Guangheng Gao, Wei Chen
Summary: The portable electrochemical uricometer with uricase-packaged nanoflowers can continuously monitor uric acid in urine non-invasively and efficiently, requiring only a small amount of urine for each test. The method shows specificity and accuracy, with results from volunteers with different dietary choices being used as effective evidence for uric acid control.
BIOSENSORS & BIOELECTRONICS
(2022)
Article
Chemistry, Analytical
Pravanjan Malla, Hao-Ping Liao, Chi-Hsien Liu, Wei-Chi Wu
Summary: A highly sensitive magneto immunosensor for detecting parathyroid hormone (PTH) was successfully developed using MBs modified by APBA and a disposable electrode, allowing for rapid point-of-care testing for PTH. The sensor exhibited a linear detection range and low limits of detection by both voltammetry and impedance spectroscopy, showcasing its potential for practical diagnostic applications.
JOURNAL OF ELECTROANALYTICAL CHEMISTRY
(2021)
Article
Biophysics
A. Amine, F. Arduini, D. Moscone, G. Palleschi
BIOSENSORS & BIOELECTRONICS
(2016)
Article
Chemistry, Analytical
Laura Micheli, Claudia Mazzuca, Antonio Palleschi, Giuseppe Palleschi
MICROCHEMICAL JOURNAL
(2016)
Article
Chemistry, Analytical
C. Mazzuca, L. Micheli, R. Lettieri, E. Cervelli, T. Coviello, C. Cencetti, S. Sotgiu, S. Iannuccelli, G. Palleschi, A. Palleschi
MICROCHEMICAL JOURNAL
(2016)
Article
Chemistry, Analytical
G. Volpe, E. Delibato, L. Fabiani, E. Pucci, S. Piermarini, A. D'Angelo, F. Capuano, D. De Medici, G. Palleschi
Article
Chemistry, Analytical
Fabiana Arduini, Daniela Neagu, Valeria Pagliarini, Viviana Scognamiglio, Maria Antonietta Leonardis, Emanuela Gatto, Aziz Amine, Giuseppe Palleschi, Danila Moscone
Article
Chemistry, Analytical
Joost L. D. Nelis, Davide Migliorelli, Safiye Jafari, Silvia Generelli, Javier Lou-Franco, J. Pablo Salvador, M. Pilar Marco, Cuong Cao, Christopher T. Elliott, Katrina Campbell
Review
Chemistry, Analytical
A. S. Tsagkaris, J. L. D. Nelis, G. M. S. Ross, S. Jafari, J. Guercetti, K. Kopper, Y. Zhao, K. Rafferty, J. P. Salvador, D. Migliorelli, G. I. J. Salentijn, K. Campbell, M. P. Marco, C. T. Elliot, M. W. F. Nielen, J. Pulkrabova, J. Hajslova
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2019)
Article
Chemistry, Analytical
Ye Tang, Konstantinos Petropoulos, Felix Kurth, Hui Gao, Davide Migliorelli, Olivier Guenat, Silvia Generelli
Article
Chemistry, Analytical
A. S. Tsagkaris, D. Migliorelli, L. Uttl, D. Filippini, J. Pulkrabova, J. Hajslova
Summary: In this study, a microfluidic paper-based analytical device (μPAD) was developed to rapidly screen for the toxic chlorpyrifos metabolite, chlorpyrifos-oxon, in human serum. The μPAD demonstrated rapid results within 10 minutes, cost efficiency, and a low detection capability, making it potentially applicable for point-of-need use. Additionally, the μPAD was compared to well-established methods such as an in-house BChE microplate assay and liquid chromatography tandem mass spectrometry.
Article
Chemistry, Analytical
Joost L. D. Nelis, Davide Migliorelli, Lea Muehlebach, Silvia Generelli, Linda Stewart, Christopher T. Elliott, Katrina Campbell
Summary: A novel electrochemical immunosensor was developed for the detection of marine biotoxins DA and OA. The sensor showed excellent stability and sensitivity, achieving low LODs for both toxins in buffer and mussel extract.
Review
Food Science & Technology
Safiye Jafari, Julian Guercetti, Ariadni Geballa-Koukoula, Aristeidis S. Tsagkaris, Joost L. D. Nelis, M. -Pilar Marco, J. -Pablo Salvador, Arjen Gerssen, Jana Hajslova, Chris Elliott, Katrina Campbell, Davide Migliorelli, Loic Burr, Silvia Generelli, Michel W. F. Nielen, Shana J. Sturla
Summary: Standard methods for chemical food safety testing rely on mass spectrometry in official laboratories, but there is a demand for portable devices due to the need for sampling and expensive equipment. Recent technological advancements include smartphone-based, microfluidic chip-based, and paper-based devices, as well as the potential application of portable mass spectrometers in food testing.
Article
Biophysics
Ye Tang, Hui Gao, Felix Kurth, Loic Burr, Konstantinos Petropoulos, Davide Migliorelli, Olivier T. Guenat, Silvia Generelli
Summary: LFIA is a well-established technique that provides immediate results, but suffers from high limits of detection. Introduction of a novel cellulose nanofiber aerogel material into LFIA strips has been shown to improve the detection sensitivity by extending the sample flow time.
BIOSENSORS & BIOELECTRONICS
(2021)
Article
Chemistry, Analytical
Safiye Jafari, Loic Burr, Davide Migliorelli, Roger Galve, M. Pilar Marco, Katrina Campbell, Chris Elliott, Michele Suman, Shana J. Sturla, Silvia Generelli
Summary: Considering the complexities and speed of modern food chains, there is an increasing demand for point-of-need detection of food contaminants, particularly highly regulated chemicals and carcinogens such as aflatoxin B1. We report a user-friendly smartphone-based magneto-immunosensor on carbon black modified electrodes for point-of-need detection of aflatoxin B1 in cereals. The assay demonstrated a low limit of detection and high reproducibility, and its applicability was validated on the basis of EU guidelines. An open-source android application, AflaESense, was designed to provide a simple interface for data analysis.
ANALYTICA CHIMICA ACTA
(2022)
Article
Biochemical Research Methods
Wanda Cimmino, Davide Migliorelli, Sima Singh, Antonella Miglione, Silvia Generelli, Stefano Cinti
Summary: MicroRNAs (miRNAs) are important clinical biomarkers for cancer diagnosis and prognosis. In this study, an electrochemical detection platform based on a screen-printed gold electrode was developed for the detection of miRNA. The platform showed high selectivity and sensitivity in both standard solutions and urine samples.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Proceedings Paper
Engineering, Electrical & Electronic
L. Lombardo, S. Generelli, N. Tscharner, D. Migliorelli, N. Donato
2016 IEEE SENSORS APPLICATIONS SYMPOSIUM (SAS 2016) PROCEEDINGS
(2016)
Article
Chemistry, Analytical
Mengmeng Guo, Na Luo, Yueling Bai, Zhenggang Xue, Qingmin Hu, Jiaqiang Xu
Summary: A porous heterostructure WO3-C/In2O3 was designed and prepared for a miniature H2 sensor, which showed higher response value, lower operating temperature, fast response-recovery speed, and low limit of detection.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Feng Hu, Hui Hu, Yuting Li, Xiaohui Wang, Xiaowen Shi
Summary: Arsenic contamination in water bodies is a significant health risk. This study developed a chitosan-catechol modified electrode for rapid and accurate detection of trace amounts of arsenic. The modified electrode demonstrated good detection capability and resistance to ionic interference, making it suitable for in situ detection.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Yantao Zhang, Qian Liu, Tao Tian, Chunhua Xu, Pengli Yang, Lianju Ma, Yi Hou, Hui Zhou, Yongjun Gan
Summary: In this study, a lysosome-targeting buffering fluorogenic probe (Lyso-BFP) was designed and synthesized, demonstrating excellent photostability, pH specificity, and responsiveness to lysosomal acidification in living cells. The performance of Lyso-BFP in pH sensing was attributed to the inhibition of the photo-induced electron transfer process. Lyso-BFP allowed for wash-free imaging and long-term real-time monitoring of lysosome pH changes based on its off-on fluorescence behavior and buffer strategy.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Wei Cai, Wenbo Sun, Jiayue Wang, Xiaokui Huo, Xudong Cao, Xiangge Tian, Xiaochi Ma, Lei Feng
Summary: In this study, a near-infrared fluorescent probe HCBG was developed for imaging of alpha-GLC. HCBG exhibited excellent selectivity and sensitivity towards alpha-GLC in complex bio-samples, and showed good cell permeability for in situ real-time imaging. Through the high-throughput screening system established by HCBG, a natural alpha-GLC inhibitor was successfully isolated and identified. This study provides a novel fluorescence visualization tool for discovering and exploring the biological functions of diabetes-related gut microbiota, and a high-throughput screening approach for alpha-GLC inhibitor.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Trey W. Pittman, Xi Zhang, Chamindie Punyadeera, Charles S. Henry
Summary: Heart failure is a growing epidemic and a significant clinical and public health problem. Researchers have developed a portable and affordable diagnostic device for heart failure that can be used at the point-of-care, providing a valid alternative to current diagnostics approaches.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Anders O. Tjell, Barbara Jud, Roland Schaller-Ammann, Torsten Mayr
Summary: An optical hydrogen peroxide sensor based on catalytic degradation and the detection of produced oxygen is presented. The sensor offers higher resolution and better sensitivity at lower H2O2 concentrations. By removing O2 from the sample solution, a more sensitive O2 sensor can be used for measurement. The sensor has been successfully applied in a flow-through cell to measure H2O2 concentration in different flow rates.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Seong Jae Kim, Ji-hun Jeong, Gaabhin Ryu, Yoon Sick Eom, Sanha Kim
Summary: Surface-enhanced Raman spectroscopy (SERS) is a high-sensitivity, label-free detection method with various analytical applications. Researchers have developed a hydrophobic SERS substrate based on engineered carbon nanotube arrays (CNT-SERS) and studied the role of structural design at both micro and nanoscales. The substrate demonstrated controlled self-enrichment capability and enhanced sensitivity, with a significant increase in the SERS signal. The study also proposed a theoretical model and a concentration strategy inspired by plants for analyte deposition on microarrays.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Dan Zhao, Renjun Jiang, Xiaoqiang Liu, Subbiah Alwarappan
Summary: In this study, a novel ternary composite material was constructed by assembling cerium vanadate nanorods on reduced graphene oxide-microcrystalline cellulose nanosheets, and it was used for real-time monitoring of the concentration of superoxide anions in vivo. The ternary composite showed excellent conductivity, large surface area, and abundant active sites, leading to a wider linear range, high sensitivity, low detection limit, and fast response time for superoxide anion detection.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Tengfei Wang, Liwen Wang, Guang Wu, Dating Tian
Summary: In this study, a covalent organic framework material TaTp-COF with porous and uniform spheres was successfully prepared via hydrothermal reaction, and it was found to significantly enhance the aggregation-induced emission (AIE) of berberine. The unique emission properties of berberine on TaTp-COF were studied and utilized for the sensitive detection of berberine.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Lin Li, Yilei Ding, Lei Xu, Shuoran Chen, Guoliang Dai, Pengju Han, Lixin Lu, Changqing Ye, Yanlin Song
Summary: In this study, a novel TTI based on a ratiometric fluorescent nanosensor is designed, which has the advantages of high accuracy and low cost. Experimental and theoretical investigations confirm its pH responsiveness and demonstrate its good sensitivity and reliability. By monitoring the total volatile basic nitrogen, this TTI can accurately predict food spoilage and can be adaptively modified for different types of food. The TTI based on this nanosensor enables visual monitoring of food quality.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Fangju Chen, Xueting Wang, Wei Chen, Chenwen Shao, Yong Qian
Summary: Lung cancer is the second most common malignant tumor worldwide. Drug resistance in lung cancer leads to treatment failure and recurrence in majority of patients. This study developed a fluorescent prodrug that can be activated in cancer cells to release drugs, and its signal can be tracked by imaging. It shows a unique autophagy-driven ferroptosis effect, indicating its potential for targeting drug-resistant cancer cells.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Weichao Li, Qiming Yuan, Zhangcheng Xia, Xiaoxue Ma, Lifang He, Ling Jin, Xiangfeng Chu, Kui Zhang
Summary: This study developed a high-performance gas sensor for formaldehyde detection by modifying ZnSnO3 with ZnO QDs and SnO2 QDs. The modified sensor showed improved sensing response and lower working temperature. The presence of ZnO QDs formed rich heterojunctions, increased surface area, and provided oxygen deficiency for formaldehyde sensing reaction, thus enhancing the sensor performance. This research provides an alternative method to enhance the sensing properties of MOS by QDs modification.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Joung-Il Moon, Eun Jung Choi, Younju Joung, Jin-Woo Oh, Sang-Woo Joo, Jaebum Choo
Summary: A novel nanoplasmonic substrate was developed for biomedical applications, which showed strong hot spots for detecting biomarkers at low concentrations. The substrate, called AuNPs@M13, was made by immobilizing 60 nm gold nanoparticles onto the surface of an M13 bacteriophage scaffold. It demonstrated higher sensitivity and lower limit of detection compared to commercially available assays.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Ning Li, Ya Zhang, Ying Xu, Xiaofang Liu, Jian Chen, Mei Yang, Changjun Hou, Danqun Huo
Summary: The molecular subtype of breast cancer guides treatment and drug selection. Invasive tests can promote cancer cell metastasis, so the development of high-performance, low-cost diagnostic tools for cancer prognosis is crucial. Liquid biopsy techniques enable noninvasive, real-time, dynamic, multicomponent, quantitative, and long-term observations at the cellular, genetic, and molecular levels. A Cu-Zr metal-organic framework (MOF) nanoenzyme with monatomic Cu attachment has been synthesized and proven to have high catalytic performance. The sensor constructed using this nanoenzyme shows potential for accurate classification of breast cancer serum samples.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Jeongmin Kim, Hyemin Kim, Seunghyun Park, Hyeonaug Hong, Yong Jae Kim, Jiyong Lee, Jaeho Kim, Seung-Woo Cho, Wonhyoung Ryu
Summary: This study presents a method to fabricate independently functioning microneedle (MN) electrodes with narrow intervals for high precision electrochemical sensing. The optimized mixture of photocurable polymer and single-wall carbon nanotubes was used to mold single composite MNs, which were then attached to pre-patterned electrodes. Plasma etching and electropolymerization were performed to enhance the electrochemical activity, and Prussian blue and glucose oxidase were electrodeposited on the MNs for glucose detection. The MN electrodes showed good sensitivity and linearity, and the feasibility of glucose detection was demonstrated in an in vivo mouse study.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
