4.8 Article

Recent advances in biosensors based on enzyme inhibition

期刊

BIOSENSORS & BIOELECTRONICS
卷 76, 期 -, 页码 180-194

出版社

ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2015.07.010

关键词

Biosensor; Enzyme inhibition; Nanomaterials; Analytical applications

资金

  1. European Commission [613844]
  2. Marie Curie Actions People IRSES [294901]
  3. NATO Science for Peace project [SFP 984173]
  4. Italian Minister of Defence

向作者/读者索取更多资源

Enzyme inhibitors like drugs and pollutants are closely correlated to human and environmental health, thus their monitoring is of paramount importance in analytical chemistry. Enzymatic biosensors represent cost-effective, miniaturized and easy to use devices; particularly biosensors based on enzyme inhibition are useful analytical tools for fast screening and monitoring of inhibitors. The present review will highlight the research carried out in the last 9 years (2006-2014) on biosensors based on enzyme inhibition. We underpin the recent advances focused on the investigation in new theoretical approachs and in the evaluation of biosensor performances for reversible and irreversible inhibitors. The use of nanomaterials and microfluidic systems as well as the applications of the various biosensors in real samples is critically reviewed, demonstrating that such biosensors allow the development of useful devices for a fast and reliable alarm system. (C) 2015 Elsevier B.V. All rights reserved.

作者

我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。

评论

主要评分

4.8
评分不足

次要评分

新颖性
-
重要性
-
科学严谨性
-
评价这篇论文

推荐

Article Chemistry, Analytical

An origami paper-based electrochemical biosensing platform for quality control of agri-food waste in the valorization strategy

Noemi Colozza, Erika Di Meo, Angelica Mucaria, Danila Moscone, Fabiana Arduini

Summary: The development of a novel paper-based origami device for assessing the amount of glucosinolate and glucose in food waste samples has been presented. The device consists of two paper-based biosensors that work synergistically in a multiplexed detection. It offers a sustainable approach for evaluating the quality of waste samples and ensuring the correct management of re-used food product waste.

MICROCHIMICA ACTA (2022)

Article Chemistry, Analytical

A Proof-of-Concept Electrochemical Cytosensor Based on Chlamydomonas reinhardtii Functionalized Carbon Black Screen-Printed Electrodes: Detection of Escherichia coli in Wastewater as a Case Study

Amina Antonacci, Fabiana Arduini, Raouia Attaallah, Aziz Amine, Maria Teresa Giardi, Viviana Scognamiglio

Summary: In this study, a proof-of-concept algal cytosensor utilizing immobilized Chlamydomonas reinhardtii on carbon black nanomodified electrodes was developed for electrochemical quantification of bacteria in wastewater. The sensor demonstrated the ability to sense the oxygen produced by the algae, resulting in increased current when exposed to higher concentrations of bacteria. The sensor showed good detection capability for Escherichia coli in both standard solutions and real wastewater samples, with a linear range of response and a low limit of detection. The research also indicated the potential use of the cytosensor as an early warning analytical system for heavy loads of pathogens in wastewater, highlighting its importance in water quality monitoring.

BIOSENSORS-BASEL (2022)

Review Biophysics

Multiplexed sensing techniques for cardiovascular disease biomarkers- A review

Veerappan Mani, Ceren Durmus, Walaa Khushaim, Daisy Camargo Ferreira, Suna Timur, Fabiana Arduini, Khaled Nabil Salama

Summary: Cardiovascular diseases are a leading cause of death worldwide, and timely diagnosis relies on accurate determination of cardiac biomarkers. Multiplexed biosensors targeting multiple markers have emerged as a promising alternative to single-marker analysis. This study critically reviewed various multiplexed biosensing approaches, categorized them by signal readouts, and discussed their advantages, limitations, and future prospects.

BIOSENSORS & BIOELECTRONICS (2022)

Article Electrochemistry

Carbon-black combined with TiO2 and KuQ as sustainable photosystem for a reliable self-powered photoelectrochemical biosensor

Vincenzo Mazzaracchio, Roberta Marrone, Mattia Forchetta, Federica Sabuzi, Pierluca Galloni, Mingqing Wang, Ahmet Nazligul, Kwang-Leong Choy, Fabiana Arduini, Danila Moscone

Summary: This article reports a photoelectrochemical biosensor based on a printed electrode modified with carbon black (CB). The sensor is designed using CB, TiO2, and KuQ dye, and shows the advantage of portable laser configuration under visible light. After optimization of fabrication and working conditions, the sensor demonstrates good performance in NADH measurement and successfully detects ethanol in white wine samples.

ELECTROCHIMICA ACTA (2022)

Article Chemistry, Analytical

Mask assistance to colorimetric sniffers for detection of Covid-19 disease using exhaled breath metabolites

Mohammad Mahdi Bordbar, Hosein Samadinia, Ali Hajian, Azarmidokht Sheini, Elham Safaei, Jasem Aboonajmi, Fabiana Arduini, Hashem Sharghi, Pegah Hashemi, Hosein Khoshsafar, Mostafa Ghanei, Hasan Bagheri

Summary: According to the World Health Organization, a large number of people have been infected or died from Covid-19. Researchers have introduced an optical sniffer that can differentiate between Covid-19 patients, healthy individuals, and those who have recovered by detecting exhaled breath metabolites. The method has an accuracy rate of 80%-84% and shows a linear correlation with disease severity and viral load.

SENSORS AND ACTUATORS B-CHEMICAL (2022)

Review Chemistry, Analytical

New frontiers in microfluidics devices for miRNA analysis

Patricia Khashayar, Sallam Al-Madhagi, Mostafa Azimzadeh, Viviana Scognamiglio, Fabiana Arduini

Summary: miRNA detection using microfluidics-based devices is an important innovation in biology and modern medicine. Microfluidics-based biosensors are rapid and affordable analytical devices for high-throughput quantification of miRNAs, overcoming the limitations of conventional methodologies. This review summarizes recent advancements in microfluidic devices for miRNA sample handling, manipulation, and measurement, and discusses their advantages, limitations, and future trends in the field.

TRAC-TRENDS IN ANALYTICAL CHEMISTRY (2022)

Review Chemistry, Physical

Electrochemical paper-based devices: When the simple replacement of the support to print ecodesigned electrodes radically improves the features of the electrochemical devices

Fabiana Arduini

Summary: Paper-based electrochemical (bio)sensors have sustainable features and versatile advantages, such as the combination with vertical microfluidics, acting as a reservoir to deliver smart electrochemical (bio)sensors, and detecting target analytes in liquid, aerosol, and solid samples.

CURRENT OPINION IN ELECTROCHEMISTRY (2022)

Article Biochemical Research Methods

A smart paper-based electrochemical sensor for reliable detection of iron ions in serum

Vincenzo Mazzaracchio, Neda Bagheri, Francesco Chiara, Luca Fiore, Danila Moscone, Simona Roggero, Fabiana Arduini

Summary: The fast-growing demand for user-friendly and affordable analytical tools in healthcare is driving the development of reliable platforms for customized therapy based on individual health conditions. We developed a cost-effective paper-based electrochemical sensor for quantifying iron ions in serum to ensure correct supplement administration. The sensor, modified with a nanocomposite of carbon black and gold nanoparticles, showed high sensitivity and accuracy in iron detection. This paper-based sensor has great potential for future applications in precision medicine for iron-deficiency diseases.

ANALYTICAL AND BIOANALYTICAL CHEMISTRY (2023)

Article Biochemistry & Molecular Biology

Study on Molecularly Imprinted Polymers Obtained Sonochemically for the Determination of Aflatoxins in Food

Sara Palmieri, Dounia Elfadil, Federico Fanti, Flavio Della Pelle, Manuel Sergi, Aziz Amine, Dario Compagnone

Summary: Researchers proposed an alternative strategy to extract aflatoxins AFB1, AFB2, AFG1, and AFG2 from complex food samples using molecularly imprinted polymers (MIPs) synthesized via a low-cost and rapid sonochemical polymerization. The MIPs-based solid phase extraction showed appreciable recovery rates (65-90%) and good reproducibility (RSD <= 6%, n = 3) on 17 dietary supplements, and exhibited selectivity towards other mycotoxins compared to commercial immunoaffinity columns. This strategy offers a more affordable and better-performing alternative to traditional immunoaffinity columns.

MOLECULES (2023)

Article Chemistry, Analytical

Paper-Based Colorimetric Detection of miRNA-21 Using Pre-Activated Nylon Membrane and Peroxidase-Mimetic Activity of Cysteamine-Capped Gold Nanoparticles

Maliana El Aamri, Hasna Mohammadi, Aziz Amine

Summary: A new genosensor based on paper and nanozyme activity of Cys/AuNPs was developed to detect picomolar concentrations of miRNA-21. The Cys/AuNPs catalyze the colorimetric reaction of H2O2 and TMB, producing a measurable blue color. The method showed a good linear range between 1 pM and 1 nM with a detection limit of 0.5 pM, and it was effective, selective, and sensitive for miRNA-21 detection. The proposed method also demonstrated acceptable accuracy in the analysis of a human serum sample.

BIOSENSORS-BASEL (2023)

Article Chemistry, Analytical

Selective Sensing in Microbial Fuel Cell Biosensors: Insights from Toxicity-Adapted and Non-Adapted Biofilms for Pb(II) and Neomycin Sulfate Detection

Abdelghani Ghanam, Sebastien Cecillon, Hasna Mohammadi, Aziz Amine, Francois Buret, Naoufel Haddour

Summary: This study introduces the utilization of self-powered microbial fuel cell (MFC)-based biosensors for the detection of biotoxicity in wastewater. A novel approach is introduced, capitalizing on the adaptive capabilities of anodic biofilms to enhance the selectivity of MFC biosensors.

MICROMACHINES (2023)

Article Chemistry, Analytical

Magnetic Molecularly Imprinted Chitosan Combined with a Paper-Based Analytical Device for the Smartphone Discrimination of Tryptophan Enantiomers

Abdelhafid Karrat, Juan Jose Garcia-Guzman, Jose Maria Palacios-Santander, Aziz Amine, Laura Cubillana-Aguilera

Summary: The separation of enantiomers is critical in pharmaceutical development. In this study, a simple and effective chiral detection strategy was developed for distinguishing tryptophan (TRP) enantiomers. The method involved the use of a magnetic molecularly imprinted chitosan (MMIC) and a nitrocellulose membrane integrated with D-TRP, which induced a noticeable color change in gold nanoparticles (AuNPs) for chiral analysis. The method exhibited a detection limit of 3.3 μM and showed good recovery rates in serum samples.

BIOSENSORS-BASEL (2023)

Article Chemistry, Analytical

Development of an Innovative Colorimetric DNA Biosensor Based on Sugar Measurement

Maliana El Aamri, Yasmine Khalki, Hasna Mohammadi, Aziz Amine

Summary: The development of a colorimetric genosensor for the detection of DNA, RNA, and DNA intercalator molecules is presented in this work. The biosensor demonstrates high sensitivity, good selectivity, and linear response over a wide concentration range. It offers a potential tool for sensitive and selective detection in various applications, including virus diagnosis, genetic analysis, pathogenic bacteria monitoring, and drug discovery.

BIOSENSORS-BASEL (2023)

Article Chemistry, Multidisciplinary

Paper card-like electrochemical platform as a smart point-of-care device for reagent-free glucose measurement in tears

Luca Fiore, Ankita Sinha, Narjiss Seddaoui, Jessica di Biasio, Federico Ricci, Goran M. Stojanovic, Fabiana Arduini

Summary: This communication presents the development of a polyvinyl chloride electrochemical system with a paper layer loaded with reagents, introducing a novel concept of a paper card-like pad for reagent-free measurement of target analytes in solution. The device successfully detects glucose in artificial tears within the range of 0.2-2 mM, with a detection limit of 50 mu M by simply adding the tears to the paper card-like pad. This innovative configuration surpasses the current state of the art, expanding the application range of paper in smart analytical device design.

CHEMICAL COMMUNICATIONS (2023)

Article Chemistry, Multidisciplinary

Ultrasound-Assisted Synthesis of an Advanced Sorbent Based on Amphiphilic Poly(Methacrylic Acid-co-Styrene) Modified Magnetic Nanoparticles for Removal and Analytical Determination of Dicofol

Abdelhafid Karrat, Abderrahman Lamaoui, Fabiana Arduini, Aziz Amine

Summary: In this study, a magnetic amphiphilic poly(methacrylic acid-co-styrene) material was synthesized for the removal or analytical determination of dicofol in water. The material showed fast and high adsorption capacity, and high selectivity towards dicofol.

CHEMISTRY AFRICA-A JOURNAL OF THE TUNISIAN CHEMICAL SOCIETY (2023)

Article Biophysics

An integrated centrifugal microfluidic strategy for point-of-care complete blood counting

Reza Khodadadi, Manouchehr Eghbal, Hamideh Ofoghi, Alireza Balaei, Ali Tamayol, Karen Abrinia, Amir Sanati-Nezhad, Mohamadmahdi Samandari

Summary: This paper introduces an integrated portable centrifugal microfluidic system that automates cell and fluid manipulation for complete blood counting (CBC) analysis at the point-of-care (POC). The system utilizes a specially designed microfluidic disc for cell separation, solution metering and mixing, and cell counting, and is equipped with a custom script for automated quantification of cells. The proposed method shows a strong correlation with the gold standard hematology analyzer for various blood parameters. The portable system offers simplicity, affordability, and low power consumption, making it a potential solution for improving healthcare delivery in resource-limited settings and remote areas.

BIOSENSORS & BIOELECTRONICS (2024)

Article Biophysics

Immunity testing against COVID-19 from blood by an IoT-enabled and AI-controlled multiplexed microfluidic platform

Nabil H. Bhuiyan, Joon S. Shim

Summary: Developing herd immunity is crucial for changing the course of the COVID-19 pandemic. An AI-driven point-of-care testing platform has been proposed for analyzing the body's response to SARS-CoV-2, and it has been successfully used for quantifying viral proteins and antibodies in blood samples. A data-receptive web-based dashboard system has also been developed for real-time analysis of herd immunity progress.

BIOSENSORS & BIOELECTRONICS (2024)

Article Biophysics

Influence of shear stress on electroactive biofilm characteristics and performance in microbial fuel cells

Alexiane Godain, Timothy M. Vogel, Pascal Fongarland, Naoufel Haddour

Summary: This study provides comprehensive insights into the intricate relationship between shear stress and electroactive biofilms in microbial fuel cells, highlighting the pivotal role of shear stress in influencing the growth kinetics, electrical performance, and physical structure of the biofilms. The study also emphasizes the complexities of extracellular electron transfer mechanisms and the need for complementary metaproteomic analyses.

BIOSENSORS & BIOELECTRONICS (2024)

Article Biophysics

Cheap and portable paper chip with terrific oxidase-like activity and SERS enhancement performance for SERS-colorimetric bimodal detection of intracellular glutathione

Linjie Wang, Yixin Chen, Yang Ji, Shujun Zheng, Fei Wang, Caolong Li

Summary: A paper-based biosensor incorporating surface-enhanced Raman spectroscopy (SERS) and colorimetric detection has been developed for efficient quantification of intracellular glutathione (GSH). The biosensor exhibits excellent selectivity, stability, and precision, with low detection limits in both SERS and colorimetric modes. It has been successfully utilized for intracellular GSH detection and validated against a commercial GSH assay kit.

BIOSENSORS & BIOELECTRONICS (2024)

Article Biophysics

Tracking the EMT-like phenotype switching during targeted therapy in melanoma by analyzing extracellular vesicle phenotypes

Quan Zhou, Jing Wang, Zhen Zhang, Alain Wuethrich, Richard J. Lobb, Matt Trau

Summary: This study presents a biosensor for sensitive detection of EMT-associated biomarkers on extracellular vesicles (EVs) surfaces during targeted therapy. Through longitudinal monitoring of patients, the biosensor shows its ability to identify the EMT-like phenotype switching on circulating EVs during the development of resistance.

BIOSENSORS & BIOELECTRONICS (2024)

Review Biophysics

Protease detection in the biosensor era: A review

Pratika Rai, Sabrina N. Hoba, Celine Buchmann, Robert J. Subirana-Slotos, Christian Kersten, Tanja Schirmeister, Kristina Endres, Bernd Bufe, Alexey Tarasov

Summary: Proteases have been proposed as potential biomarkers for various pathological conditions. The development of protease biosensors offers a more efficient way to investigate dysregulated proteases. This review article presents different optical and electrochemical detection methods for designing biosensors for all major protease families.

BIOSENSORS & BIOELECTRONICS (2024)

Article Biophysics

Controllable self-assembled DNA nanomachine enable homogeneous rapid electrochemical one-pot assay of lung cancer circulating tumor cells

Chengxin Liu, Xu Shen, Li Yan, Runlian Qu, Yue Wang, Yaqin He, Zixuan Zhan, Piaopiao Chen, Feng Lin

Summary: In this study, a homogeneous rapid electrochemical aptasensor was developed to quantitatively detect CTCs in lung cancer patients. The aptasensor utilized a DNA nanosphere structure and a complementary aptamer to specifically detect mucin 1 as a marker for CTCs. The assay exhibited high specificity and sensitivity, and the results were consistent with other detection methods.

BIOSENSORS & BIOELECTRONICS (2024)

Article Biophysics

Therapeutic drug monitoring mediated by the cooperative chemical and electromagnetic effects of Ti3C2TX modified with Ag nanocubes

Danni Xue, Xing Dai, Jialong Zhao, Jiayao Zhang, Huan Liu, Kui Liu, Tao Xu, Chenjie Gu, Xingfei Zhou, Tao Jiang

Summary: In this study, a dual-enhancement SERS substrate based on Ti3C2TX and Ag nanocubes was fabricated for precise quantification of ritonavir and ibrutinib in serum. The formation of numerous electromagnetic hotspots between Ag nanocubes facilitated effective photo-induced charge transfer. The composite substrate showed excellent sensitivity, achieving low detection limits and high recoveries, making it promising for monitoring and identification of clinical blood drug concentration.

BIOSENSORS & BIOELECTRONICS (2024)

Article Biophysics

CRISPR-Cas12a powered hybrid nanoparticle for extracellular vesicle aggregation and in-situ microRNA detection

Tenghua Zhang, Zihui Xie, Xiaohe Zheng, Yuxin Liang, Yao Lu, Hankang Zhong, Feiyang Qian, Yuqing Zhu, Ruiting Sun, Yan Sheng, Jiaming Hu

Summary: This study reports a technology based on cationic lipid-polymer hybrid nanoparticles for efficient extracellular vesicle (EV) enrichment and in-situ detection of internal microRNAs. The technology demonstrates high EV enrichment efficiency and sensitive internal RNA detection, making it potentially useful for early pancreatic cancer diagnosis.

BIOSENSORS & BIOELECTRONICS (2024)

Article Biophysics

Facile synthesis of dual-ligand europium-metal organic gels for ratiometric electrochemiluminescence detecting I27L gene

Wenjie Dai, Gaoxu Chen, Xiaoyan Wang, Shujun Zhen, Chengzhi Huang, Lei Zhan, Yuanfang Li

Summary: In this study, a novel metal organic gel (MOG) with dual electrochemiluminescence (ECL) properties was prepared by simple mixing. The MOG exhibited strong and stable anodic and cathodic ECL signals. By utilizing this MOG, an ECL resonance energy transfer (ECL-RET) biosensor was constructed for ultrasensitive detection of a specific gene. The study developed a straightforward technique for obtaining a single luminescent material with dual signals and expanded the analytical application of MOGs in the realm of ECL.

BIOSENSORS & BIOELECTRONICS (2024)

Article Biophysics

The use of biological fluids in microfluidic paper-based analytical devices (μPADs): Recent advances, challenges and future perspectives

Lais Canniatti Brazaca, Amanda Hikari Imamura, Rodrigo Vieira Blasques, Jessica Rocha Camargo, Bruno Campos Janegitz, Emanuel Carrilho

Summary: The use of microfluidic paper-based analytical devices (muPADs) for medical diagnosis is a growing trend due to their low cost, easy use, simple manufacturing, and potential for application in low-resource settings. This review focuses on the advances in muPADs for medical diagnostics, discussing their use in detecting various biomarkers in common human biofluids. The challenges of biomarker detection in each sample are examined, along with innovative techniques to overcome these limitations. The commercialization difficulties of muPADs are also considered, along with future trends such as wearable devices and integrated platforms.

BIOSENSORS & BIOELECTRONICS (2024)

Article Biophysics

Development of a peptide microarray-based metal-enhanced fluorescence assay for ultrasensitive detection of multiple matrix metalloproteinase activities by using a gold nanorod-polymer substrate

Minghong Jian, Xudong Sun, Hua Zhang, Xiaotong Li, Shasha Li, Zhenxin Wang

Summary: Matrix metalloproteinases (MMPs) are attractive biomarkers for cancer diagnosis and treatment, but their low abundance in biological samples, especially in the early stages of tumors, makes it challenging to precisely analyze MMP activities. In this study, a peptide microarray-based metal-enhanced fluorescence assay (PMMEFA) is proposed as a sensitive and specific method to simultaneously detect MMP-1, -2, -3, -7, -9, and -13 activities. The PMMEFA showed excellent sensitivity and was capable of detecting MMP activities in various matrices.

BIOSENSORS & BIOELECTRONICS (2024)

Article Biophysics

Plasmonic digital PCR for discriminative detection of SARS-CoV-2 variants

Kyung Ho Kim, Eunsu Ryu, Zinah Hilal Khaleel, Sung Eun Seo, Lina Kim, Yong Ho Kim, Hyun Gyu Park, Oh Seok Kwon

Summary: We have developed a novel strategy for discriminative detection of SARS-CoV-2 variants using the plasmonic photothermal effect of gold nanofilms and digital polymerase chain reaction (dPCR) technology. With this method, we were able to detect the delta and omicron variants with high sensitivity within 25 minutes from COVID-19 patients' clinical samples, making it a rapid and accurate point-of-care testing tool.

BIOSENSORS & BIOELECTRONICS (2024)

Article Biophysics

A wearable and flexible lactic-acid/O2 biofuel cell with an enhanced air-breathing biocathode

Zepeng Kang, Yuanming Wang, Haiyan Song, Xueli Wang, Job Zhang, Zhiguang Zhu

Summary: By designing a wearable and flexible lactic-acid/O2 EBFC with an air-breathing biocathode, the limitations of biocathode are effectively solved. The optimal performance conditions are determined through experiments, and the EBFC is successfully applied to power a low-power device.

BIOSENSORS & BIOELECTRONICS (2024)

Article Biophysics

Gas-responsive two-dimensional metal-organic framework composites for trace visualization of volatile organic compounds

Huayun Chen, Zhiheng You, Yuhui Hong, Xiao Wang, Mingming Zhao, Yushi Luan, Yibin Ying, Yixian Wang

Summary: This study developed a colorimetric sensor array using gas-responsive two-dimensional metal-organic framework (MOF) composites for highly sensitive detection of volatile organic compounds (VOCs). The dye/Zn-2(bim)(4) composites-based sensor arrays showed enhanced sensitivity and anti-interference properties. The findings demonstrate the potential use of dye/Zn-2(bim)(4) sensor arrays for early perception of plant diseases.

BIOSENSORS & BIOELECTRONICS (2024)