Article
Chemistry, Physical
Xinyi Chen, Junfeng Chen, Nawal M. Alghoraibi, Danielle A. Henckel, Ruixian Zhang, Uzoma O. Nwabara, Kenneth E. Madsen, Paul J. A. Kenis, Steven C. Zimmerman, Andrew A. Gewirth
Summary: A Cu-polyamine hybrid catalyst was developed to significantly enhance the selectivity for ethylene production in CO2 electrochemical conversion. Incorporating polyamine altered surface reactivity, leading to improved product selectivity at high current densities.
Article
Materials Science, Biomaterials
Li-Song Zhang, Yi-Lun Yin, Lei Wang, Ying Xia, SungJu Ryu, Zhen Xi, Lu-Yuan Li, Zhi-Song Zhang
Summary: A Nap-G/Biotin/ANA-FFpYGK-NTA probe has been designed for specific and rapid enrichment of target proteins in living cells by self-assembling into nanofibers, with functionalities like specific binding to 6His-tag and promotion of self-assembly. The probe demonstrates suitability for real-time tracking and isolation of His-tagged proteins.
JOURNAL OF MATERIALS CHEMISTRY B
(2021)
Article
Chemistry, Multidisciplinary
Angus S. Mackay, Joshua W. C. Maxwell, Max J. Bedding, Sameer S. Kulkarni, Stephen A. Byrne, Lucas Kambanis, Mihai V. Popescu, Robert S. Paton, Lara R. Malins, Anneliese S. Ashhurst, Leo Corcilius, Richard J. Payne
Summary: This study describes the development of an electrochemical selenoetherification (e-SE) platform for the selective and efficient functionalization of polypeptides under mild conditions. The unique reactivity of selenocysteine allows the formation of stable selenoether-linked conjugates through electrochemical reactions. The utility of this platform is demonstrated through the modification of a FDA-approved cancer drug and the assembly of complex anti-HER2 affibody conjugates. The functionalized affibodies show potential applications in in vitro imaging and targeting of HER2-positive tumor cells.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Article
Chemistry, Analytical
Xuerao Ning, Takanobu Yasuda, Tetsuya Kitaguchi, Hiroshi Ueda
Summary: This study developed an antibody-based His-tag sensor Quenchbody (Q-body) for quick detection and monitoring of C-terminally His-tagged recombinant protein. By introducing Fab-based Q-body and mutations of tyrosine to tryptophan in the heavy chain CDR region, the fluorescence response and detection sensitivity were improved. This technology can effectively help speed up the monitoring of production processes and screening of higher-yield target protein variants.
Article
Chemistry, Analytical
Ying Chen, Bin Liu, Zhengbo Chen, Xia Zuo
Summary: Enrichment techniques for phosphopeptides often involve interactions between phosphate groups and metal ions or metal oxides. In this study, an electrochemical sensor based on TiO2 NPs was designed to detect phosphopeptides in protein samples pretreated with AuNPs, showing high sensitivity and a wide linear concentration range. Additionally, TiO2 and GO composite materials were synthesized to enhance the detection capability, with the uniformly dispersed anatase crystal TiO2 and GO composite-modified electrode exhibiting a lower detection limit.
ANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Multidisciplinary
Wancun Zhang, Pin Zhang, Ying Liang, Weyland Cheng, Lifeng Li, Huanmin Wang, Zhidan Yu, Yan Liu, Xianwei Zhang
Summary: In this study, a ratiometric hairpin DNA probe based electrochemical biosensor was developed for rapid and sensitive detection of Hg2+. The biosensor showed high ultrasensitivity, specificity, repeatability, and reproducibility in quantifying trace Hg2+. It exhibited a broad linear dynamic range and high sensitivity, and demonstrated excellent selectivity towards Hg2+ ions in the presence of other metal ions. The biosensor has been successfully applied in the detection of Hg2+ in various samples with good recovery rate and small relative standard deviations.
Article
Biotechnology & Applied Microbiology
Nan Wang, Guiying Pan, Shimin Guan, Shaofeng Rong, Dapeng Wang, Zhiyong Gao, Peng Tian, Qianqian Li
Summary: In this study, an electrochemical biosensor based on a screen-printed carbon electrode was developed for the rapid detection of human noroviruses (HuNoVs). The biosensor showed high sensitivity and specificity, and could detect different genogroups and genotypes of HuNoVs. The detection limit of HuNoVs in clinical fecal samples was 10(4) genomic copies/mL. The results could be obtained within 1 hour.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Engineering, Chemical
Ana Freitas, Lucilia Domingues, Tatiana Q. Aguiar
Summary: This study evaluated the feasibility of using bare silica as a matrix for protein purification/immobilization, and the results showed that unmodified silica matrices can effectively purify His-tagged proteins, with the combination of tags being advantageous for immobilization purposes.
SEPARATION AND PURIFICATION TECHNOLOGY
(2022)
Article
Chemistry, Physical
Shreya Pramanik, Jan Steinkuehler, Rumiana Dimova, Joachim Spatz, Reinhard Lipowsky
Summary: His-tagged molecules can be attached to lipid bilayers via anchor lipids for biofunctionalization. Two fluorescent His-tagged molecules, GFP and FITC, were studied in this research. The brightness of membrane-bound 6H-GFP was found to exceed that of membrane-bound 6H-FITC, contrary to their quantum yields in solution. The membrane fluorescence was also measured as a function of the molar concentration of fluorophores, leading to the determination of equilibrium dissociation constants and a strong pH-dependence of membrane fluorescence.
Article
Chemistry, Analytical
Anna C. R. Moco, Joao A. S. Neto, Dayane D. de Moraes, Pedro H. Guedes, Jessica G. Brussasco, Jose M. R. Flauzino, Luiz F. G. Luz, Marcia M. C. N. Soares, Joao M. Madurro, Ana G. Brito-Madurro
Summary: This study presents a new methodology for constructing screen-printed electrodes for the detection of HIV RNA, utilizing carbon ink and modified gold nanoparticles. The biosensor demonstrated sensitivity in detecting HIV RNA within the clinically relevant concentration range, allowing for quantification of viral load.
MICROCHEMICAL JOURNAL
(2021)
Article
Materials Science, Biomaterials
Muhammad Rezki, Ni Luh Wulan Septiani, Muhammad Iqbal, Suksmandhira Harimurti, Poetro Sambegoro, Damar Rastri Adhika, Brian Yuliarto
Summary: Amine-functionalized Cu-MOF nanospheres were successfully synthesized and optimized for HBsAg detection. The immobilization of a bioreceptor allowed for accurate detection of the hepatitis B infection biomarker.
JOURNAL OF MATERIALS CHEMISTRY B
(2021)
Article
Polymer Science
Unchalee Kongintr, Benchaporn Lertanantawong, Chamras Promptmas
Summary: In this study, a label-free electrochemical biosensor for reliable detection of serum homocysteine (Hcy) was developed using a molecularly imprinted polymer (MIP) and nanocomposite. The Hcy-MIP biosensor showed high sensitivity, with a linear response range of 5.0 to 150 μM and a limit of detection of 1.2 μM. It demonstrated low cross-reactivity with other compounds and achieved good recoveries and repeatability. This novel biosensor offers a new and effective method for Hcy assay compared to existing techniques.
Article
Chemistry, Analytical
Yuanyuan Chang, Sai Xu, Yunrui Li, Wenxi Hu, Hang Li, Ruo Yuan, Yaqin Chai
Summary: In this study, a DNA three-way junction with multiple recognition regions was engineered to act as an efficient and fast DNA walker for electrochemical detection of miRNA-182-5p. This walker could walk on the electrode surface without confinement, significantly improving detection efficiency and sensitivity.
ANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Analytical
Anabel Villalonga, Concepcion Parrado, Raul Diaz, Alfredo Sanchez, Beatriz Mayol, Paloma Martinez-Ruiz, Diana Vilela, Reynaldo Villalonga
Summary: We have developed a novel labeling strategy for electrochemical aptasensors based on enzymatic marking via supramolecular host-guest interactions. This approach utilizes a modified DNA aptamer and a covalently attached neoglycoconjugate to achieve highly selective detection of specific biomarkers.
Article
Chemistry, Analytical
M. S. Kumar, Ruchira Nandeshwar, Shailesh B. Lad, Kirti Megha, Maheshwar Mangat, Adrian Butterworth, Charles W. Knapp, Mara Knapp, Paul A. Hoskisson, Damion K. Corrigan, Andrew C. Ward, Kiran Kondabagil, Siddharth Tallur
Summary: A low-cost electrochemical DNA biosensor based on PCB electrodes is presented for monitoring virus amplicons in wastewater, offering advantages such as high sensitivity, reusability, and ease of cleaning. It is capable of detecting SARSCoV-2 and other pathogens, providing valuable insights for researchers in the field.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Review
Chemistry, Analytical
Kamila Malecka, Edyta Mikula, Elena E. Ferapontova
Summary: The improvement in outcomes for various types of cancer in recent years is attributed to early tumor detection and widespread use of screening tests, with non-invasive, fast, and accurate diagnostic devices playing a crucial role. Biosensors utilizing aptamers in cancer diagnosis offer advantages such as in-vitro aptamer production, cost-effective chemical synthesis, and thermal stability, while electrochemical biosensing approaches provide sensitive, accurate, and affordable sensing methods. This review evaluates the design strategies and analytical performance of electrochemical aptamer-based biosensors for cancer diagnosis, highlighting their future prospects in cancer diagnostics.
Article
Biochemistry & Molecular Biology
Gopal Palla, Kamila Malecka, Wim Dehaen, Jerzy Radecki, Hanna Radecka
Summary: This report presents an electrochemical immunosensor for monitoring A beta(1-42) fibrils, fabricated with stepwise assembly of the sensing platform and successfully applied in different media. The immunosensor shows better sensitivity in the presence of APP and moderate response towards A beta(1-42) oligomers, demonstrating good selectivity.
BIOELECTROCHEMISTRY
(2021)
Article
Biochemistry & Molecular Biology
Kamila Malecka, Balwinder Kaur, D. Andrea Cristaldi, Clarissa S. Chay, Iwona Mames, Hanna Radecka, Jerzy Radecki, Eugen Stulz
Summary: A cobalt-porphyrin modified DNA was used as an electrochemical marker to detect specific DNA sequences, comparing the performance of gold and silver nanoparticles in oligonucleotide sensors. Surprisingly, both types of sensors showed similar overall performance, with direct electron transfer between the porphyrin and the electrode. The silver system displayed better DNA economy and cost-effectiveness compared to gold, making it more suitable for mass production of highly sensitive DNA sensors.
BIOELECTROCHEMISTRY
(2021)
Article
Chemistry, Multidisciplinary
Ji Hyeon Kim, Peter Verwilst, Miae Won, Junhyoung Lee, Jonathan L. Sessler, Jiyou Han, Jong Seung Kim
Summary: Breast cancer stem cells (BCSCs) are a major contributor to the unfavorable outcomes seen in many breast cancer patients, as they are more prevalent in hypoxic environments and refractory to traditional chemotherapy. A small molecule construct named AzCDF has been developed to target BCSCs effectively in the hypoxic tumor environment, reducing tumor growth and migration. This is the first time a CSC-targeting small molecule has been shown to prevent tumorigenesis in an animal model.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2021)
Article
Chemistry, Applied
Na Hee Kim, Rae Hyung Kang, Peter Verwilst, Dokyoung Kim
Summary: The fluorescent nanoprobe AgP-1 has been developed for detecting silver ions in biological media, showing high selectivity, sensitivity, and significant fluorescence enhancement upon forming complex with silver ions. The probe also demonstrates fast response time and superior biocompatibility, with successful visualization of silver ions in cancer cells and bacteria. This indicates the potential of AgP-1 as a practical sensor for silver ions detection in biomedical applications.
Review
Biochemistry & Molecular Biology
Edyta Mikula, Jaroslav Katrlik, Ligia R. Rodrigues
Summary: This review discusses the important clinical biomarkers for Parkinson's Disease and their physiological role, and introduces the application of innovative aptasensors based on electrochemical techniques. The future directions and analytical platforms are also discussed.
CURRENT MEDICINAL CHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Monika Zareba-Koziol, Michal Burdukiewicz, Aleksandra Wyslouch-Cieszynska
Summary: Human S100B is a small multifunctional protein that plays a role in the biology of various tissues and is associated with several diseases. This study focuses on its extracellular activity and its interaction with the RAGE receptor, and shows that the presence of extracellular S100B leads to changes in intracellular S-nitrosylation. These findings highlight the importance of considering S-nitrosylation in future research and therapy development for S100B and RAGE receptor-related diseases.
Article
Engineering, Biomedical
Jusung An, Peter Verwilst, Hira Aziz, Jinwoo Shin, Sungsu Lim, Ilwha Kim, Yun Kyung Kim, Jong Seung Kim
Summary: The pathological origin of Alzheimer's disease is still unknown, but the selective visualization of beta-amyloid is crucial for understanding the disease. In this study, two new fluorescent compounds were designed to have high affinity for beta-amyloid and were successfully used for fluorescent staining of amyloid plaques in cortical brain tissue. These compounds expand the family of beta-amyloid-sensitive fluorophores.
BIOACTIVE MATERIALS
(2022)
Editorial Material
Biochemistry & Molecular Biology
Edyta Mikula, Halina Kaczmarek
CURRENT MEDICINAL CHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Magdalena Polakowska, Kamil Steczkiewicz, Roman H. Szczepanowski, Aleksandra Wysouch-Cieszynska
Summary: S100A8 and S100A9 are small calcium-binding proteins with important roles in signaling, regulation, and defense. Through structural mass spectrometry methods and simulations, this study compares the conformational properties of wildtype full-length S100A8 and S100A9 subunits in biologically relevant homo-and heterodimers and higher oligomers. It provides insights into the functional responses of these proteins to changing environmental conditions and identifies key factors contributing to the stability of the oligomers. These findings lay the foundation for further research on the functions and modulation of S100A8 and S100A9 complexes for basic science and therapies.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2023)
Review
Materials Science, Multidisciplinary
Edyta Mikula, Kamila Malecka-Baturo
Summary: Neurodegenerative diseases (NDs) are a significant global health problem, and there is a high demand for methods to detect ND biomarkers early. The complexity of neuronal degeneration and the heterogeneity of patient populations present challenges for the development of early diagnostic tools. Various analytical technologies, including biosensors, have been developed to meet this challenge. Electrochemical aptasensors, in particular, have emerged as a powerful tool for detecting ND biomarkers due to their low cost, simple design, and advances in nanomaterials. This review summarizes and compares the design of electrochemical aptasensors for detecting ND biomarkers like alpha-synuclein, amyloid beta peptide, tau protein, and human cellular prion protein, and highlights innovative strategies for enhancing sensitivity and selectivity. There is still a need for low-cost, fast, and user-friendly systems for early ND detection.
Editorial Material
Biochemistry & Molecular Biology
Edyta Mikula, Halina Kaczmarek
CURRENT MEDICINAL CHEMISTRY
(2023)
Article
Chemistry, Multidisciplinary
Margaux Billen, Dominique Schols, Peter Verwilst
Summary: Since the discovery of the first biologically active chemokines in the late 1980s, these messenger proteins and their receptors have become targets for drug discovery efforts. Recent research has focused on a highly druggable, intracellular, allosteric binding site that partially overlaps with the G protein binding site, and the development of antagonists.
CHEMICAL COMMUNICATIONS
(2022)
Article
Chemistry, Multidisciplinary
Jihee Cho, Jinwoo Shin, Minjung Kang, Peter Verwilst, Changhyun Lim, Hanbin Yoo, Jeung Gon Kim, Xingcai Zhang, Chang Seop Hong, Jong Seung Kim, Sanghee Kim
Summary: A novel calix[n]triazolium was synthesized, which showed excellent selectivity for detecting AMP through turn-on fluorescence. This macrocyclic system is the first to discriminate AMP from ADP and ATP based on fluorescence changes.
CHEMICAL COMMUNICATIONS
(2021)