期刊
SCIENTIA HORTICULTURAE
卷 145, 期 -, 页码 94-101出版社
ELSEVIER
DOI: 10.1016/j.scienta.2012.08.010
关键词
Apple; V-ATPase gene MdVHA-B; Transgenic tomato; ABA; Drought stress
类别
资金
- National Public Benefit (Agricultural) Research Foundation of China [200903044]
- National High Technology Research and Development Program of China [2011AA100204]
- Changjiang Scholars and Innovative Research Team in University [IRT1155]
The survival of plant cells strongly depends on maintaining or adjusting the activity of the V-ATPase under drought conditions. In this study, a V-ATPase gene, VHA-B, was identified in an apple cDNA library, and an analysis of the deduced MdVHA-B protein sequence showed that it clusters with VHA-B proteins of other species. The expression of MdVHA-B was induced by abiotic stress, including abscisic acid (ABA), salinity, drought and low and high temperatures. Furthermore, the over-expression of MdVHA-B in transgenic tomato plants resulted in a high tolerance to drought stress, reduced relative water loss and malondialdehyde contents and increased levels of free proline and H+-ATPase activity compared to the wild-type plants (WT). Additionally, it has similar sensibility to exogenous ABA in transgenic MdVHA-B tomato seeds and seedlings, while more sensitive to polyethylene glycol in transgenic tomato compared with WT. This study indicates that MdVHA-B plays an important role in drought stress, yet may act independently of the ABA signaling pathway. (C) 2012 Elsevier B.V. All rights reserved.
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