期刊
SCIENTIA HORTICULTURAE
卷 130, 期 4, 页码 708-714出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.scienta.2011.07.029
关键词
EF-hand calcium-binding protein; Embryo abortion; Seedlessness; Tobacco rattle virus (TRV); Vitis vinifera; VvCBP1
类别
资金
- National Public Benefit (Agricultural) Research Foundation of China [200903044]
- earmarked fund for Modern Agro-industry Technology Research System [Nycytx-30-zp-06]
EF-hand calcium-binding protein (CBP) gene plays a critical role during embryonic development in animals, but it remains unclear whether this gene possesses similar function in plants. In this study, a novel CBP gene was isolated from Vitis vinifera cv. Thompson Seedless and designated as VvCBP1. Using a combination of sequence structural investigation, expression pattern analysis of different embryo development in grapevine, and silencing assay of VvCBP1 homolog in tomato, the functional role of VvCBP gene in embryo development of seedless grapevine was studied. Structural investigations showed that VvCBP1 is 907 bp in length, and contains two EF-hand motifs. Expression pattern of this gene is different in embryo between seedless and seeded grapevine species. Moreover, the silencing of homology gene led to the seeds number significantly decreased in tomato. These data demonstrate clearly that VvCBP1 plays an important role in embryo development of seedless grapevine. (C) 2011 Elsevier B.V. All rights reserved.
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