Article
Multidisciplinary Sciences
Etienne de Coulon, Christian Dellenbach, Stephan Rohr
Summary: The LSSE system allows for comprehensive intracellular electrical recordings on cells undergoing strain events, providing stable conditions for studying mechanically activated ion currents. This system offers advanced insights into mechanosensitive ion channel function and allows for quantitative comparisons among different types of channels and cells.
Article
Neurosciences
Zijing Chen, Maggie Kerwin, Orlaith Keenan, Craig Montell
Summary: This study identified the structural requirements for TRP channel function in Drosophila photoreceptor cells, including the roles of the S4-S5 linker and the TRP domain, as well as the differential requirements for two highly conserved motifs in the TRP domain for activation and protein stability. Genetic complementation experiments revealed an intrasubunit interaction between the S4-S5 linker and the S5 segment in Drosophila photoreceptor cells, which contributes to channel activation.
JOURNAL OF NEUROSCIENCE
(2021)
Review
Biochemistry & Molecular Biology
Thomas K. Smylla, Krystina Wagner, Armin Huber
Summary: Phosphorylation of rhodopsin plays a diverse role in vertebrate and fly visual systems, contributing to the inactivation of activated receptors and facilitating endocytosis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Cell Biology
Suhasini Rajan, Christian Schremmer, Jonas Weber, Philipp Alt, Fabienne Geiger, Alexander Dietrich
Summary: Members of the TRP superfamily, particularly TRPV4, play crucial roles in respiratory system physiology and pathology, serving as potential therapeutic targets for diseases like asthma, lung fibrosis, edema, and infections.
Review
Physiology
Mengning Zheng, Niyati A. Borkar, Yang Yao, Xianwei Ye, Elizabeth R. Vogel, Christina M. Pabelick, Y. S. Prakash
Summary: Mechanosensitive channels play a crucial role in cell sensing and response, and the mechanical activation of ion channels and downstream signaling pathways is important for maintaining physiological homeostasis and the development of diseases. Understanding TRP and Piezo channels in the lung can provide new insights for pulmonary disease treatment.
FRONTIERS IN PHYSIOLOGY
(2023)
Review
Physiology
Rebekah George, Ralf Stanewsky
Summary: Circadian clocks are 24-hour oscillators regulated by cell-autonomous clock gene expression, which communicate with the environment through light and temperature cues to synchronize internal time with external time. In fruit flies, both light and temperature fluctuations can synchronize circadian clocks independently, with temperature input relying on sensory cells in the peripheral nervous system.
FRONTIERS IN PHYSIOLOGY
(2021)
Article
Developmental Biology
Vishakha Vishwakarma, Thao Phuong Le, Se Yeon Chung
Summary: During the invagination of Drosophila embryonic salivary gland, the GPCR Smog transduces the Fog signal to regulate Rho kinase accumulation and myosin activation, thereby controlling apical constriction. Smog also plays unexpected roles in maintaining epithelial integrity and organizing cortical actin.
Article
Multidisciplinary Sciences
Mitsumasa Koyanagi, Baoguo Shen, Takashi Nagata, Lanfang Sun, Seiji Wada, Satomi Kamimura, Eriko Kage-Nakadai, Akihisa Terakita
Summary: Optical control of GPCR signaling is a valuable approach for understanding and controlling GPCR-based physiologies. In this study, the performance of two bistable opsins, MosOpn3 and LamPP, in optical control in vivo was systematically investigated using Caenorhabditis elegans. The results demonstrated the advantages of MosOpn3 and LamPP in light-induced behavior and color-dependent control of behavior, respectively. Molecular engineering was also applied to enhance the usability of these opsins. These findings provide numerous strategies for optical control of GPCR-based physiologies and GPCR signaling.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Neurosciences
Chia-Ling Hsieh, Yun Yao, Vsevolod V. Gurevich, Jeannie Chen
Summary: Arr1 plays a key role in facilitating the dephosphorylation of rhodopsin in vivo. Mice lacking Arr1 show delayed dephosphorylation of rhodopsin, which cannot be explained by cell stress or downregulation of protein phosphatase 2A.
JOURNAL OF NEUROSCIENCE
(2022)
Article
Multidisciplinary Sciences
Rita Gutorov, Ben Katz, Baruch Minke
Summary: This research focuses on studying the bi-stable photopigment in Drosophila using random mutagenesis and proposes simple and robust methods for screening mutant flies. Electrophysiological methods, such as measuring early receptor potential and prolonged depolarizing afterpotential, allow direct measurement of functional photopigment levels and the discovery of novel signaling proteins related to phototransduction.
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
(2022)
Article
Multidisciplinary Sciences
Rong-Chang Li, Laurie L. Molday, Chih-Chun Lin, Xiaozhi Ren, Alexander Fleischmann, Robert S. Molday, King-Wai Yau
Summary: This study provides evidence that the ligand-triggered GPCR pathway in mammalian olfactory transduction produces less than one downstream effector when an odorant-receptor molecule is complexed with its most effective odorants. This finding suggests a potential paradigm shift in our understanding of GPCR signaling.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Neurosciences
Beiying Liu, Feng Qin
Summary: The study unveils the molecular basis underlying the high temperature dependence of the heat-sensitive TRPV2 channel, revealing a key molecular region in the proximal N-terminus that dictates the heat activation profile of the channel.
JOURNAL OF PHYSIOLOGY-LONDON
(2021)
Review
Cell Biology
Akito Nakao, Yoshihiro Matsunaga, Katsumi Hayashida, Nobuaki Takahashi
Summary: Psychiatric disorders are believed to be caused by complex and diverse factors, with oxidative stress playing a crucial role in the pathogenesis of major disorders such as bipolar disorder, depression, anxiety disorder, and schizophrenia. Calcium channels are essential for neuronal functions, and the investigation of redox-sensitive TRP channels could lead to the development of new therapeutic strategies for psychiatric disorders.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Review
Pharmacology & Pharmacy
Nathalie Fuentes, Morgan McCullough, Reynold A. Panettieri, Kirk M. Druey
Summary: Asthma is a prevalent disorder characterized by chronic lung inflammation and reversible airways obstruction, with features including increased airway narrowing, excessive mucus secretion, airway remodeling, and airways hyperresponsiveness. Environmental factors such as allergens, viruses, and air pollution can promote asthma, leading to symptoms like cough, wheezing, and shortness of breath. G-protein-coupled receptors play a central role in asthma, with various treatments targeting these receptors to regulate airway smooth muscle tone and lung inflammation.
PHARMACOLOGY & THERAPEUTICS
(2021)
Article
Multidisciplinary Sciences
Bingxue Li, Songling Li, Honglan Zheng, Zhiqiang Yan
Summary: Research shows that in Drosophila larvae, the MET currents in Cho neurons are mediated by two TRPV channels, NAN and IAV, with the C-terminal residues 857 through 990 of IAV regulating the kinetics of MET currents. Additionally, the NompC channel contributes to the adaptation of auditory transduction currents.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Multidisciplinary Sciences
Huawei Tong, Qian Li, Zi Chao Zhang, Yi Li, Junhai Han
SCIENTIFIC REPORTS
(2016)
Article
Neurosciences
Yi Li, Zikai Zhou, Xinwang Zhang, Huawei Tong, Peipei Li, Zi Chao Zhang, Zhengping Jia, Wei Xie, Junhai Han
JOURNAL OF NEUROSCIENCE
(2013)
Article
Biotechnology & Applied Microbiology
Huawei Tong, Jia Huang, Qingquan Xiao, Bingbing He, Xue Dong, Yuanhua Liu, Xiali Yang, Dingyi Han, Zikang Wang, Wenqin Ying, Runze Zhang, Yu Wei, Xuchen Wang, Chunlong Xu, Yingsi Zhou, Yanfei Li, Minqing Cai, Qifang Wang, Mingxing Xue, Guoling Li, Kailun Fang, Hainan Zhang, Hui Yang
Summary: Several engineered Cas13 variants have been found to have efficient targeted degradation activity with reduced collateral effects in mammalian cells. These variants show no off-target effects and cell growth arrest, making them suitable for targeted RNA degradation in basic research and therapeutic applications.
NATURE BIOTECHNOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Huawei Tong, Xuchen Wang, Yuanhua Liu, Nana Liu, Yun Li, Jiamin Luo, Qian Ma, Danni Wu, Jiyong Li, Chunlong Xu, Hui Yang
Summary: We developed an A-to-C and A-to-T transversion base editor called AYBE by fusing ABE with MPG. We also engineered AYBE variants that have higher transversion editing activity (up to 72%) at specific genomic loci.
NATURE BIOTECHNOLOGY
(2023)
Article
Multidisciplinary Sciences
Huawei Tong, Nana Liu, Yinghui Wei, Yingsi Zhou, Yun Li, Danni Wu, Ming Jin, Shuna Cui, Hengbin Li, Guoling Li, Jingxing Zhou, Yuan Yuan, Hainan Zhang, Linyu Shi, Xuan Yao, Hui Yang
Summary: Current base editors can only deaminate cytosine (C) or adenine (A), lacking the ability to edit guanine (G) or thymine (T). In this study, a deaminase-free glycosylase-based guanine base editor (gGBE) was developed by fusing Cas9 nickase with engineered N-methylpurine DNA glycosylase protein (MPG). Through mutagenesis of MPG and screening using an intron-split EGFP reporter, gGBE with engineered MPG showed over 1500-fold increase in G editing efficiency. Furthermore, this gGBE demonstrated high base editing efficiency and G-to-T or G-to-C conversion ratio in both cultured human cells and mouse embryos.
NATIONAL SCIENCE REVIEW
(2023)
Article
Biochemistry & Molecular Biology
Qian Li, Yi Li, Xiao Wang, Junxia Qi, Xi Jin, Huawei Tong, Zikai Zhou, Zi Chao Zhang, Junhai Han
