Article
Cell Biology
Sheila Bandyopadhyay, Xiao Zhang, Andrea Ascura, Karen L. Edelblum, Edward M. Bonder, Nan Gao
Summary: In this study, a novel host regulator of enteric Salmonella infections, CDC42EP1, was identified. It was found that CDC42EP1 interacts with SEPTIN-7 and Villin, and its localization and engagement with bacteria depends on host CDC42 and S. Typhimurium's capability of activating CDC42. CDC42EP1 is not required for S. Typhimurium's initial cellular entry but contributes to the pathogen's intracellular growth and replication.
JOURNAL OF CELLULAR PHYSIOLOGY
(2023)
Article
Multidisciplinary Sciences
Lingyan Jiang, Peisheng Wang, Xiaorui Song, Huan Zhang, Shuangshuang Ma, Jingting Wang, Wanwu Li, Runxia Lv, Xiaoqian Liu, Shuai Ma, Jiaqi Yan, Haiyan Zhou, Di Huang, Zhihui Cheng, Chen Yang, Lu Feng, Lei Wang
Summary: S. Typhimurium infection induces upregulated glycolysis and decreased serine synthesis in host macrophages, leading to accumulation of glycolytic intermediates that promote bacterial replication and virulence.
NATURE COMMUNICATIONS
(2021)
Review
Environmental Sciences
Sandip Ghosh, Jui-Yang Lai
Summary: Intracellular pH (pHi) measurement is crucial for understanding physiological activities and diagnosing diseases. Non-invasive nanoprobes based on nanotechnology have been developed for accurate and repeatable pHi sensing using Raman spectroscopy and fluorescence imaging. However, there are still limitations and challenges that need to be addressed. Future advancements can be made by exploring alternative nanomaterials and technologies to enhance sensitivity and reliability of pHi measurement.
ENVIRONMENTAL RESEARCH
(2023)
Article
Ecology
Bingxin Wang, Zeyu Zhang, Fugui Xu, Zixiang Yang, Zihan Li, Danyu Shen, Limin Wang, Huijun Wu, Tao Li, Qing Yan, Qi Wei, Xiaolong Shao, Guoliang Qian
Summary: This study discovered a mechanism in which a soil bacterium triggers another bacterium to produce antifungal antibiotics, leading to protection of soybean seedlings from fungal infection. This interaction highlights the diversity of microbial interactions in soil and provides insights into the role of antibacterial weapons in biotrophic microbial interactions.
Review
Chemistry, Analytical
Ying Wen, Ning Jing, Fangjun Huo, Caixia Yin
Summary: Fluorescent probes and fluorescence microscopy are indispensable tools in biomedical research, enabling the exploration of cellular chemical factors and their physiological functions and pathological changes. Intracellular pH value is a critical factor, while different organelles possess unique acid-base homeostasis involved in important physiological processes.
Article
Multidisciplinary Sciences
Qi Yan, Wenqing Zhang, Mingqun Lin, Omid Teymournejad, Khemraj Budachetri, Jeffrey Lakritz, Yasuko Rikihisa
Summary: Etf-3 is a bacterial protein that induces ferritinophagy to facilitate pathogen iron capture, allowing the bacterium to acquire iron for survival and proliferation in the host cell.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Spectroscopy
Yutao Jia, Yanting Shen, Yanyan Zhu, Jing Wang
Summary: This study reports a novel covalent organic framework as a pH-responsive fluorescent probe for lysosomal pH imaging in cells. The probe exhibits good pH-responsive properties and can effectively monitor pH fluctuations and changes in lysosomes.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2022)
Article
Biochemical Research Methods
Hui Chen, Caixia Luo, Shangtao Zhang
Summary: Exosomes have been highly studied for their potential as cancer diagnostic targets and therapeutic agents. A new method using SERS technology has been demonstrated for imaging exosomes intracellularly and sensing pH simultaneously. This technique allows for tracking exosomal distribution within cells and quantitative pH sensing, providing insights into interactions between exosomes and cells.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Analytical
Jorge Espina-Casado, Tania Fontanil, Alfonso Fernandez-Gonzalez, Santiago Cal, Alvaro J. Obaya, Marta Elena Diaz-Garcia, Rosana Badia-Laino
Summary: Carbon dots were synthesized from glutathione and surface-modified with beta-Cyclodextrin and FP, showing potential applications as drug carriers. The fluorescence changes allowed distinction of cancer cells with no alterations in cell morphology, enabling bioimaging and cancer diagnosis.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Review
Microbiology
Tian Xie, Guifeng Liu, Jiayi Ma, Yaonan Wang, Ran Gao, Shizhong Geng, Xinan Jiao, Paul Barrow
Summary: By screening a library of FDA-approved drugs, the study found that Nifuratel is an effective and least toxic drug for combating Salmonella infection in macrophages. In mice, it provided 60% protection against lethal Salmonella Enteritidis infection and reduced bacterial numbers in tissues. It also showed efficacy against different serovars of Salmonella, multidrug-resistant strains, and other bacterial pathogens like Listeria and Shigella. Additionally, it reduced the production of certain proteins in infected macrophages, which could enhance the inflammatory response against Salmonella.
MICROBIOLOGY SPECTRUM
(2023)
Article
Microbiology
Tian Xie, Guifeng Liu, Jiayi Ma, Yaonan Wang, Ran Gao, Shizhong Geng, Xinan Jiao, Paul Barrow
Summary: This study evaluated the antibacterial effect of a range of FDA-approved drugs against macrophages infected with Salmonella. The nitrofuran antibiotic Nifuratel showed the highest antibacterial activity with minimal toxicity to macrophages, and was effective against different strains of Salmonella and other pathogens.
MICROBIOLOGY SPECTRUM
(2023)
Review
Immunology
Debipreeta Bhowmik, Fanxiu Zhu
Summary: Sensing viral constituents is crucial for host immune defense against viruses. Host DNA sensors have been discovered and their mechanisms elucidated, but herpesviruses employ diverse strategies to evade host DNA sensing.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Veronica Loyo-Celis, Devendra Patel, Shridhar Sanghvi, Kamalpreet Kaur, Devasena Ponnalagu, Yang Zheng, Sahej Bindra, Harmeet Rireika Bhachu, Isabelle Deschenes, Shubha Gururaja Rao, Harpreet Singh
Summary: CLIC6 is a protein implicated in various cancers and known to interact with dopamine receptors. This study characterized the biophysical properties of CLIC6 and found it to localize to the plasma membrane, exhibit high permeability to chloride ions, and be regulated by pH and redox potential.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2023)
Article
Multidisciplinary Sciences
Bram Van den Bergh, Hannah Schramke, Joran Elie Michiels, Tom E. P. Kimkes, Jakub Leszek Radzikowski, Johannes Schimpf, Silke R. Vedelaar, Sabrina Burschel, Liselot Dewachter, Nikola Loncar, Alexander Schmidt, Tim Meijer, Maarten Fauvart, Thorsten Friedrich, Jan Michiels, Matthias Heinemann
Summary: Antibiotic persisters are phenotypic variants within an isogenic bacterial population that are transiently tolerant to antibiotic treatment. This study provides evidence that cytoplasmic acidification, amplified by a compromised respiratory complex I, can act as a signaling hub for perturbed metabolic homeostasis in antibiotic persisters.
NATURE COMMUNICATIONS
(2022)
Article
Immunology
Shuangshuang Ma, Lingyan Jiang, Jingting Wang, Xiaoqian Liu, Wanwu Li, Shuai Ma, Lu Feng
Summary: Salmonella enterica serovar Typhimurium utilizes host macrophages as a niche for survival and replication, controlling the expression of flagellar genes to minimize host immune response. The transcriptional regulator AsiR positively regulates flagellar gene expression and its downregulation contributes to S. Typhimurium replication and systemic infection. Acidic pH in macrophages serves as a signal for AsiR and flagellar gene downregulation.
Article
Microbiology
Ethel Bayer-Santos, Charlotte H. Durkin, Luciano A. Rigano, Andreas Kupz, Eric Alix, Ondrej Cerny, Elliott Jennings, Mei Liu, Aindrias S. Ryan, Nicolas Lapaque, Stefan H. E. Kaufmann, David W. Holden
CELL HOST & MICROBE
(2016)
Article
Microbiology
Xiu-Jun Yu, Mei Liu, David W. Holden
Article
Microbiology
Nathalie Rolhion, R. Christopher D. Furniss, Grzegorz Grabe, Aindrias Ryan, Mei Liu, Sophie A. Matthews, David W. Holden
Article
Microbiology
Charlotte Odendall, Nathalie Rolhion, Andreas Foerster, John Poh, Douglas J. Lamont, Mei Liu, Paul S. Freemont, Andrew D. Catling, David W. Holden
CELL HOST & MICROBE
(2012)
Article
Cell Biology
Luis Jaime Mota, Amy E. Ramsden, Mei Liu, J. David Castle, David W. Holden
CELLULAR MICROBIOLOGY
(2009)
Article
Biochemistry & Molecular Biology
Xiu-Jun Yu, Mei Liu, Steve Matthews, David W. Holden
JOURNAL OF BIOLOGICAL CHEMISTRY
(2011)
Article
Microbiology
Jessica A. Thompson, Mei Liu, Sophie Helainet, David W. Holden
Article
Biochemistry & Molecular Biology
Piotr Mazurkiewicz, Jerry Thomas, Jessica A. Thompson, Mei Liu, Laurence Arbibe, Philippe Sansonetti, David W. Holden
MOLECULAR MICROBIOLOGY
(2008)
Article
Multidisciplinary Sciences
Sophie Helaine, Jessica A. Thompson, Kathryn G. Watson, Mei Liu, Cliona Boyle, David W. Holden
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2010)
Article
Microbiology
Xiu-Jun Yu, Grzegorz J. Grabe, Mei Liu, Luis Jaime Mota, David W. Holden
Article
Microbiology
Eric Alix, Camilla Godlee, Ondrej Cerny, Samkeliso Blundell, Romina Tocci, Sophie Matthews, Mei Liu, Jonathan N. Pruneda, Kirby N. Swatek, David Komander, Tabitha Sleap, David W. Holden
CELL HOST & MICROBE
(2020)
Article
Multidisciplinary Sciences
Anne Rytkonen, John Poh, Junkal Garmendia, Cliona Boyle, Arthur Thompson, Mei Liu, Paul Freemont, Jay C. D. Hinton, David W. Holden
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2007)
Article
Immunology
SA Linehan, A Rytkönen, XJ Yu, M Liu, DW Holden
INFECTION AND IMMUNITY
(2005)
Article
Cell Biology
John Poh, Charlotte Odendall, Ad Spanos, Cliona Boyle, Mei Liu, Paul Freemont, David W. Holden
CELLULAR MICROBIOLOGY
(2008)