Article
Biotechnology & Applied Microbiology
Julie Ming Liang, Lisa Ann Burdette, Han Teng Wong, Danielle Tullman-Ercek
Summary: Protein production using bacteria is costly and inefficient. Researchers have engineered a bacterial secretion system to improve the production process by directly secreting soluble proteins into the culture media. They explored different activation methods and created strains capable of efficiently producing heterologous proteins. They also discovered a genetic relationship between T3SS regulators, hilE and hilD, further enhancing the industrial competitiveness of protein production strains.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2023)
Article
Multidisciplinary Sciences
Eduardo Soto, Jorge E. Galan, Marfa Lara-Tejero
Summary: In this study, an extensive in vivo cross-linking strategy aided by structure modeling was used to investigate the sorting platform complex of type III secretion systems. The assembly process of this bacterial structure was mapped using identified cross-links as signatures for pairwise intersubunit interactions and systematic genetic deletions. Insights generated by this study could be used for the rational development of antivirulence strategies against medically important bacterial pathogens.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Biotechnology & Applied Microbiology
Lisa Ann Burdette, Han Teng Wong, Danielle Tullman-Ercek
Summary: In this study, the effects of different components in the growth medium on protein secretion titer were investigated. It was found that the optimal combination of carbon sources, buffers, and salts significantly increased secretion titer. This work demonstrates the importance of optimizing medium composition to enhance protein secretion titer in bacteria.
MICROBIAL CELL FACTORIES
(2021)
Article
Biochemistry & Molecular Biology
Nidhi Singh, Thales Kronenberger, Andrea Eipper, Felix Weichel, Mirita Franz-Wachtel, Boris Macek, Samuel Wagner
Summary: The study found that the salt bridges in the assembly of virulence-associated T3SS are not critical, but facilitate the incorporation of the next subunit. The conserved charged residues are crucial for type III-dependent secretion and play a key role in the function of T3SS.
JOURNAL OF MOLECULAR BIOLOGY
(2021)
Article
Biology
Owain J. Bryant, Paraminder Dhillon, Colin Hughes, Gillian M. Fraser
Summary: In addition to the gate recognition motif (GRM), a hydrophobic N-terminal signal is identified on flagellar rod and hook subunits, which are separately and sequentially recognized by the flagellar export machinery. The hydrophobic N-terminal signal may trigger the opening of the flagellar export gate.
Article
Biochemistry & Molecular Biology
Jyoti M. Gurung, Ayad A. A. Amer, Shiyun Chen, Andreas Diepold, Matthew S. Francis
Summary: YscX, an important component of the Yersinia type III secretion system, remains poorly understood in terms of its function and mode of action. This study identified an N-terminal secretion domain in YscX that promotes its own secretion and contains essential information for its function. The secretion permissiveness of YscX is closely related to the assembly of an operational secretion system.
MOLECULAR MICROBIOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Amit Meir, Kevin Mace, Yogesh Vegunta, Sunanda M. Williams, Gabriel Waksman
Summary: This review provides an update on recent advances in substrate recruitment and delivery by recruitment platforms associated with Type III, IV, and VI secretion systems in gram-negative bacteria.
TRENDS IN MICROBIOLOGY
(2023)
Article
Multidisciplinary Sciences
Stephan Wimmi, Alexander Balinovic, Hannah Jeckel, Lisa Selinger, Dimitrios Lampaki, Emma Eisemann, Ina Meuskens, Dirk Linke, Knut Drescher, Ulrike Endesfelder, Andreas Diepold
Summary: Current research shows that in gastrointestinal pathogens, the external pH plays a crucial role in regulating the assembly of T3SS cytosolic components, preventing T3SS activity in the stomach and facilitating its reactivation in the intestine.
NATURE COMMUNICATIONS
(2021)
Article
Multidisciplinary Sciences
Marc-Andre Leblanc, Morgan R. Fink, Thomas T. Perkins, Marcelo C. Sousa
Summary: The study shows that effector proteins from certain bacteria can be injected into host cells through a type III secretion system. These proteins need to be partially unfolded to pass through the narrow channel. Mechanical unfolding of the proteins revealed that they exhibit low force and high mechanical compliance, facilitating efficient secretion.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Review
Biochemistry & Molecular Biology
Julia Sanchez-Garrido, David Ruano-Gallego, Jyoti S. Choudhary, Gad Frankel
Summary: Recent studies have shown that T3SS effectors interact with each other in the host, revealing their interdependency and context-dependent essentiality, which is of great significance for understanding the pathogenesis of the pathogens.
TRENDS IN MICROBIOLOGY
(2022)
Article
Microbiology
Hanh N. Lam, Tannia Lau, Adam Lentz, Jessica Sherry, Alejandro Cabrera-Cortez, Karen Hug, Annalyse Lalljie, Joanne Engel, R. Scott Lokey, Victoria Auerbuch
Summary: The development of new antibiotics is urgently needed due to the emerging threat of antibiotic-resistant bacteria. A newly discovered isomer, 4EpDN, shows potent inhibitory effects on multiple Gram-negative bacteria's injectisome type III secretion system (T3SS), possibly by preventing complete T3SS assembly.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2021)
Article
Microbiology
Jingyan Shu, Hongtao Liu, Yang Liu, Xindi Chen, Yu Yu, Qianghua Lv, Jianfeng Wang, Xuming Deng, Zhimin Guo, Jiazhang Qiu
Summary: Tannic acid (TA) has been shown to improve survival rates and alleviate cecal pathological lesions in S. Typhimurium-infected mice, while inhibiting bacterial invasion of HeLa cells. TA may exert its anti-infection effects by controlling the expression of key genes in the T3SS.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Qingjie Li, Lianping Wang, Jingwen Xu, Shuang Liu, Zeyu Song, Tingting Chen, Xuming Deng, Jianfeng Wang, Qianghua Lv
Summary: In this study, the compound quercitrin was found to inhibit the invasion of S. Typhimurium into host cells and reduce host cell damage. It directly inhibited the function of T3SS, providing a potential antibiotic alternative against S. Typhimurium infection.
Article
Infectious Diseases
Raphael Sierocki, Bakhos Jneid, Maria Lucia Orsini Delgado, Marc Plaisance, Bernard Maillere, Herve Nozach, Stephanie Simon
Summary: Salmonella and Shigella are significant pathogens causing enteric infections, particularly in developing countries, and the development of broadly protective therapies is essential due to the emergence of antibiotic-resistant strains. By targeting needle tip proteins SipD for Salmonella and IpaD for Shigella, a monoclonal antibody was identified to have good cross-protection prophylactic efficacy, providing potential for the development of cross-protective therapeutic agents.
PLOS NEGLECTED TROPICAL DISEASES
(2021)
Article
Chemistry, Analytical
Yaron Hillman, Jenia Gershberg, Dan Lustiger, Dan Even, Dor Braverman, Yael Dror, Idan Ashur, Sefi Vernick, Neta Sal-Man, Yariv Wine
Summary: The antibiotic resistance crisis is predicted to lead to 10 million deaths annually by 2050. To address this impending challenge, the development of novel and rapid diagnostic tools is urgently needed. Through the creation of the monoclonal antibody mAb-EspB-B7, effective and stable differentiation of pathogenic bacteria is possible, showing promise as a key component in future rapid diagnostic tools.
ANALYTICAL CHEMISTRY
(2021)
Article
Microbiology
Xiu-Jun Yu, Grzegorz J. Grabe, Mei Liu, Luis Jaime Mota, David W. Holden
Article
Multidisciplinary Sciences
Xeni Miliara, Takashi Tatsuta, Jamie-Lee Berry, Sarah L. Rouse, Kuebra Solak, Dror S. Chorev, Di Wu, Carol V. Robinson, Stephen Matthews, Thomas Langer
NATURE COMMUNICATIONS
(2019)
Article
Biochemistry & Molecular Biology
Devon Sheppard, Jamie-Lee Berry, Remi Denise, Eduardo P. C. Rocha, Steve Matthews, Vladimir Pelicic
JOURNAL OF BIOLOGICAL CHEMISTRY
(2020)
Article
Microbiology
Eric Alix, Camilla Godlee, Ondrej Cerny, Samkeliso Blundell, Romina Tocci, Sophie Matthews, Mei Liu, Jonathan N. Pruneda, Kirby N. Swatek, David Komander, Tabitha Sleap, David W. Holden
CELL HOST & MICROBE
(2020)
Article
Multidisciplinary Sciences
Paul Murphy, Yingqi Xu, Sarah L. Rouse, Ellis G. Jaffray, Anna Plechanovova, Steve J. Matthews, J. Carlos Penedo, Ronald T. Hay
NATURE COMMUNICATIONS
(2020)
Article
Biology
Malgorzata Broncel, Caia Dominicus, Luis Vigetti, Stephanie D. Nofal, Edward J. Bartlett, Bastien Touquet, Alex Hunt, Bethan A. Wallbank, Stefania Federico, Stephen Matthews, Joanna C. Young, Edward W. Tate, Isabelle Tardieux, Moritz Treeck
Article
Multidisciplinary Sciences
John F. Darby, Lewis R. Vidler, Peter J. Simpson, Bissan Al-Lazikani, Stephen J. Matthews, Swee Y. Sharp, Laurence H. Pearl, Swen Hoelder, Paul Workman
SCIENTIFIC REPORTS
(2020)
Article
Microbiology
Zhihao Wang, Hongliang Wang, Nancy Mulvenna, Maximo Sanz-Hernandez, Peipei Zhang, Yanqing Li, Jia Ma, Yawen Wang, Steve Matthews, Sivaramesh Wigneshweraraj, Bing Liu
Summary: Proteins mimic DNA to occupy DNA binding sites, preventing further access. The phage protein Gp44 employs this strategy to inhibit host RNA polymerase, leading to bacterial growth inhibition. This non-specific strategy may have potential applications in developing genetically engineered phages for phage therapy targeting a range of bacterial hosts.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Chemistry, Multidisciplinary
Madhu Nagaraj, Zahra Najarzadeh, Jonathan Pansieri, Henrik Biverstal, Greta Musteikyte, Vytautas Smirnovas, Steve Matthews, Cecilia Emanuelsson, Janne Johansson, Joel N. Buxbaum, Ludmilla Morozova-Roche, Daniel E. Otzen
Summary: The study analyzes the impact of four different human chaperones on the aggregation process of functional amyloids in bacterial biofilms, showing that they primarily target primary nucleation during fibrillation rather than elongation or secondary nucleation, and the inhibition efficiency is correlated with their affinity for monomeric CsgA and FapC.
Article
Crystallography
Amit Kumar, Xu Zhang, Oscar Vadas, Fisentzos A. Stylianou, Nicolas Dos Santos Pacheco, Sarah L. Rouse, Marc L. Morgan, Dominique Soldati-Favre, Steve Matthews
Summary: A model for parasitic motility has been proposed, suggesting that parasite actin is connected to surface adhesins through a glideosome-associated connector protein (GAC). GAC is responsible for transmitting force to surface adhesins, rather than generating force. Proper connection between actin and adhesins is crucial for motility and invasion.
Article
Biochemistry & Molecular Biology
Ian R. Kelsall, Elisha H. McCrory, Yingqi Xu, Cheryl L. Scudamore, Sambit K. Nanda, Paula Mancebo-Gamella, Nicola T. Wood, Axel Knebel, Stephen J. Matthews, Philip Cohen
Summary: The E3 ligase activity of HOIL-1 is essential for preventing the accumulation of toxic polysaccharide deposits, as it ubiquitylates serine and threonine residues in proteins. HOIL-1 can monoubiquitylate glycogen and alpha 1:4-linked maltoheptaose, facilitating their removal from cells and preventing the formation of polyglucosan.
Article
Multidisciplinary Sciences
Peipei Zhang, Xiaohui Zhao, Yawen Wang, Ke Du, Zhihao Wang, Jianfeng Yu, Gang Chang, Steve Matthews, Hongliang Wang, Bing Liu
Summary: The HU protein is crucial for bacterial cell viability and serves as an antibiotic target. This study reveals that Gp46 from bacteriophage SPO1 can inhibit HU, leading to growth defects in bacteria. Through structural analysis, it is found that Gp46 occupies the DNA binding site of HU, thus preventing DNA binding. This discovery provides valuable insights into developing antibacterial and anti-malaria drugs.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Cell Biology
Kaining Zhang, Shanshan Li, Yawen Wang, Zhihao Wang, Nancy Mulvenna, Hang Yang, Peipei Zhang, Huan Chen, Yan Li, Hongliang Wang, Yongxiang Gao, Sivaramesh Wigneshweraraj, Steve Matthews, Kaiming Zhang, Bing Liu
Summary: In this study, we report a phage-encoded enolase inhibitor protein, Gp60, which can inhibit bacterial growth and peptidoglycan synthesis by disrupting the structure of enolase. This phage-inspired disassembly of enolase represents an alternative strategy for the development of antimicrobial drugs.
Article
Multidisciplinary Sciences
Georg Meisl, Catherine K. Xu, Jonathan D. Taylor, Thomas C. T. Michaels, Aviad Levin, Daniel Otzen, David Klenerman, Steve Matthews, Sara Linse, Maria Andreasen, Tuomas P. J. Knowles
Summary: The study reveals that both disease-related proteins and proteins with functional roles in biology are capable of self-replication. However, the rate of self-replication is insignificant on the biologically relevant time scale for proteins fulfilling structural roles, while disease-related proteins can self-replicate quickly within the associated disease's time scale.
Article
Biochemistry & Molecular Biology
Xeni Miliara, Takashi Tatsuta, Akinori Eiyama, Thomas Langer, Sarah L. Rouse, Steve Matthews
Summary: The PRELID-TRIAP1 family is responsible for lipid transfer in mitochondria. The lipid binding is mediated by an extended, water-mediated hydrogen bonding network. Disruption of this network by mutation R53E results in lipid release from the protein.
BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS
(2023)