Article
Microbiology
Andrew Cameron, Rupinder Mangat, Heba H. Mostafa, Samantha Taffner, Jun Wang, Ghinwa Dumyati, Richard A. Stanton, Jonathan B. Daniels, Davina Campbell, Joseph D. Lutgring, Nicole D. Pecora
Summary: Infections caused by ESBL-producing Escherichia coli are a significant health issue globally, with ST131 being the predominant clonal type. Besides bla(CTX-M-15), other ESBL variants may also be prevalent in certain regions. The identification of ST38 ESBL-producing E. coli in different regions of the United States, carrying bla(CTX-M-27) on distinct plasmid types, suggests the potential emergence of new ESBL lineages.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2021)
Article
Multidisciplinary Sciences
Hasan Ejaz, Sonia Younas, Khalid O. A. Abosalif, Kashaf Junaid, Badr Alzahrani, Abdullah Alsrhani, Abualgasim Elgaili Abdalla, Muhammad Ikram Ullah, Muhammad Usman Qamar, Sanaa S. M. Hamam
Summary: The colonization of ESBL-producing Enterobacteriaceae in animal gut microbiota poses a significant global threat, with a high prevalence of bla(CTX-M), bla(TEM), and bla(SHV) variants among enterobacteria. These genes, along with integrons, contribute to extensive drug resistance in human strains, highlighting the limited therapeutic options available.
Article
Infectious Diseases
Mohamed Belmahdi, Nadia Safia Chenouf, Abdelkrim Ait Belkacem, Sandra Martinez-Alvarez, Mario Sergio Pino-Hurtado, Zahra Benkhechiba, Samiha Lahrech, Ahcene Hakem, Carmen Torres
Summary: This study investigates the antimicrobial resistance and virulence threats of Escherichia coli isolates recovered from intestinal and fecal samples of chickens, turkeys, and sparrows. The study finds that sparrows could be a vector in the dissemination of ESBL-producing E. coli isolates to other environments. Additionally, this study reports the first detection of the bla(CTX-M-14) gene from sparrows at a global level and in turkeys in Algeria.
Article
Infectious Diseases
Selene Rebecca Boncompagni, Maria Micieli, Tiziana Di Maggio, Antonia Mantella, Anna Liz Villagran, Tatiana Briggesth Miranda, Carmen Revollo, Veronica Poma, Herlan Gamboa, Michele Spinicci, Marianne Strohmeyer, Alessandro Bartoloni, Gian Maria Rossolini, Lucia Pallecchi
Summary: This study examined the fecal carriage of CTX-M-producing Enterobacterales in school-aged children from rural areas of the Bolivian Chaco. The results showed a significant increase in the fecal carriage of CTX-M-producing Enterobacterales from 2016 to 2019. The study highlights the importance of understanding the dissemination dynamics of this resistance determinant.
INTERNATIONAL JOURNAL OF INFECTIOUS DISEASES
(2022)
Article
Microbiology
Mingxing Yang, Dong Liu, Xiaoquan Li, Chuting Xiao, Yingge Mao, Jiaqi He, Jiao Feng, Li Wang
Summary: Gram-negative bacteria producing extended-spectrum beta-lactamase (ESBL) are common and pose a significant threat in both community and hospital settings. This study examined a strain of Escherichia coli isolated from a 7-day-old infant's blood in China, which carried multiple ESBL genes and drug resistance plasmids. The findings suggest that these bacteria are highly resistant to antibiotics and capable of maintaining resistance through chromosomal location of the ESBL genes.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Microbiology
Francesca Leoni, Luca Sacchini, Silvia Pieralisi, Gabriele Angelico, Chiara Francesca Magistrali, Lucilla Cucco, Francesca Romana Massacci, Elisa Albini, Anna Duranti, Cesare Camma, Barbara Secondini, Antonio Rinaldi, Francesca Barchiesi
Summary: This study investigated the occurrence of ESBL-producing E. coli in clams collected from the Central Adriatic Sea. The results showed that approximately 3% of the samples contained ESBL-producing E. coli, with the majority of the isolates being multidrug-resistant. These findings suggest that E. coli could serve as an indicator organism for ESBL-producing E. coli in bivalves.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Microbiology
Severine Rangama, Ian D. E. A. Lidbury, Jennifer M. Holden, Chiara Borsetto, Andrew R. J. Murphy, Peter M. Hawkey, Elizabeth M. H. Wellington
Summary: In this study, strains of E. coli isolated downstream of a wastewater treatment plant in a river were found to carry multiple resistance genes, including bla(CTX-M-15) encoding an ESBL. E. coli strain 48 was shown to protect susceptible E. coli strain 33 from cefotaxime through secretion of a stable ESBL for at least 24 hours. CTX-M-15 was identified as the major secreted ESBL responsible for this protective effect, suggesting a public goods service for the survival of susceptible bacteria in the presence of cefotaxime.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2021)
Article
Microbiology
Hanshu Fang, Chung-Ho Lee, Huiluo Cao, Shuo Jiang, Simon Yung-Chun So, Cindy Wing-Sze Tse, Vincent Chi-Chung Cheng, Pak-Leung Ho
Summary: The study evaluated the NG-Test(R) CTX-M MULTI lateral flow assay (CTX-M LFA) for rapid detection of CTX-M producers in spiked and clinical blood cultures. The results showed that the CTX-M LFA could accurately detect all CTX-M producers in spiked blood cultures, but false-positive results were observed in clinical blood cultures. Therefore, the CTX-M LFA can provide a reliable and rapid detection method for CTX-M producers in blood cultures.
Article
Infectious Diseases
Amel Mhaya, Rahma Trabelsi, Sabine Aillerie, Fatima M'Zali, Dominique Begu, Slim Tounsi, Radhouane Gdoura, Corinne Arpin
Summary: Multiple ESBL-producing strains were identified in Tunisian patients, including two emerging virulent clones, ST131-C2 and ST131-C1-M27, which were found to be prevalent in both community and hospital settings.
Article
Infectious Diseases
Christopher F. Schuster, Robert E. Weber, Michael Weig, Guido Werner, Yvonne Pfeifer
Summary: This study identified a novel CTX-M ESBL gene and revealed a dynamic system of varying CTX-M gene copy numbers. The results highlight the constant emergence of new CTX-M family enzymes and demonstrate a potential evolutionary platform to generate novel ESBL variants and possibly other antibiotic resistance genes.
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2022)
Article
Microbiology
Ke Ma, Zhiyong Zong
Summary: This study investigated the mechanism of aztreonam-avibactam resistance in a carbapenem-resistant clinical strain of Escherichia coli. The results showed that E. coli strains with the YRIK insertion in PBP3, and producing CTX-M-15 or CTX-M-199 enzymes, were resistant to aztreonam-avibactam.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Infectious Diseases
Matteo Boattini, Gabriele Bianco, Davide Ghibaudo, Sara Comini, Silvia Corcione, Rossana Cavallo, Francesco Giuseppe De Rosa, Cristina Costa
Summary: Rapid detection of CTX-M ESBL-producing E. coli results in more antibiotic therapy modifications, mainly towards carbapenem-containing regimens. However, detection of CTX-M negative results leads to few therapeutic changes.
Article
Microbiology
Kinga Toth, Akos Toth, Katalin Kamotsay, Viktoria Nemeth, Dora Szabo
Summary: This study reveals that ST131 clone, particularly C2/H30Rx and C1-M27 subclades, is the dominant clone of ESBL-producing E. coli collected in a hospital in Hungary.
ANNALS OF CLINICAL MICROBIOLOGY AND ANTIMICROBIALS
(2022)
Article
Agriculture, Dairy & Animal Science
Shaqiu Zhang, Xiangyuan Guo, Yuwei Wang, Zhijun Zhong, Mingshu Wang, Renyong Jia, Shun Chen, Mafeng Liu, Dekang Zhu, Xinxin Zhao, Ying Wu, Qiao Yang, Juan Huang, Xumin Ou, Sai Mao, Qun Gao, Di Sun, Bin Tian, Anchun Cheng
Summary: We investigated the cephalosporin resistance of Escherichia coli from waterfowl in different breeding mode farms. In 2021, we isolated 200 strains of E. coli from waterfowl feces samples collected from Sichuan, Heilongjiang, and Anhui provinces. The key findings are that the cephalosporin resistance was significantly higher in intensive breeding mode farms compared to courtyard and outdoor mode farms. The blaCTX-M-55 gene was the prevalent ARGs and can be horizontally transferred through plasmids, playing a key role in the spread of cephalosporin drug resistance.
Article
Immunology
Zhen-Yu Wang, Yue Jiang, Yi-Qiao Shao, Heng-Fan Lu, Meng-Jun Lu, Xinan Jiao, Jing Wang, Qiu-Chun Li
Summary: This study investigates the prevalence and dissemination mechanism of ESBL-producing E. coli from healthy individuals in a community in Yangzhou, China. The study found nasal colonization of CTX-M-55-producing E. coli strains among healthy individuals in the community, indicating a potential risk of antimicrobial resistance dissemination through close contact or environmental transmission.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Darja Ivanova, Toni Taylor, Sarah L. Smith, Juachi U. Dimude, Amy L. Upton, Mana M. Mehrjouy, Ole Skovgaard, David J. Sherratt, Renata Retkute, Christian J. Rudolph
NUCLEIC ACIDS RESEARCH
(2015)
Article
Microbiology
Juachi U. Dimude, Anna Stockum, Sarah L. Midgley-Smith, Amy L. Upton, Helen A. Foster, Arshad Khan, Nigel J. Saunders, Renata Retkute, Christian J. Rudolph
Review
Genetics & Heredity
Juachi U. Dimude, Sarah L. Midgley-Smith, Monja Stein, Christian J. Rudolph
Article
Biochemistry & Molecular Biology
Sarah L. Midgley-Smith, Juachi U. Dimude, Toni Taylor, Nicole M. Forrester, Amy L. Upton, Robert G. Lloyd, Christian J. Rudolph
NUCLEIC ACIDS RESEARCH
(2018)
Article
Genetics & Heredity
Juachi U. Dimude, Sarah L. Midgley-Smith, Christian J. Rudolph
Article
Infectious Diseases
J. U. Dimude, S. G. B. Amyes
INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS
(2013)
Article
Genetics & Heredity
Juachi U. Dimude, Monja Stein, Ewa E. Andrzejewska, Mohammad S. Khalifa, Alexandra Gajdosova, Renata Retkute, Ole Skovgaard, Christian J. Rudolph
Article
Biochemistry & Molecular Biology
Sarah L. Midgley-Smith, Juachi U. Dimude, Christian J. Rudolph
NUCLEIC ACIDS RESEARCH
(2019)
Article
Biochemistry & Molecular Biology
Michelle Hawkins, Juachi U. Dimude, Jamieson A. L. Howard, Abigail J. Smith, Mark S. Dillingham, Nigel J. Savery, Christian J. Rudolph, Peter McGlynn
NUCLEIC ACIDS RESEARCH
(2019)
Review
Microbiology
Aisha H. Syeda, Juachi U. Dimude, Ole Skovgaard, Christian J. Rudolph
FRONTIERS IN MICROBIOLOGY
(2020)
Article
Biochemistry & Molecular Biology
Tom Killelea, Juachi U. Dimude, Liu He, Alison L. Stewart, Fiona E. Kemm, Marin Radovcic, Ivana Ivancic-Bace, Christian J. Rudolph, Edward L. Bolt
Summary: Prokaryotic Cas1-Cas2 protein complexes generate adaptive immunity by capturing and integrating MGE DNA into CRISPR sites. The chaperone DnaK inhibits DNA binding and integration by Cas1-Cas2, as well as naive adaptation resulting from chromosomal self-targeting. Removal of DnaK, mutation of its substrate binding domain, or expression of an MGE protein reverse the inhibition of naive adaptation. Additionally, Cas1 foci in living cells depend on active DNA replication and are increased in frequency in cells lacking DnaK.
NUCLEIC ACIDS RESEARCH
(2023)