Review
Biochemistry & Molecular Biology
Jamie Richards, Joel G. Belasco
Summary: Riboswitches can not only regulate translation and transcription, but also control bacterial RNA lifetimes by accelerating or impeding RNA degradation. Binding of ligand can trigger endocleavage, self-cleavage, or protect RNA from degradation by blocking access to vulnerable regions, which in turn affects translation and transcription.
MOLECULAR MICROBIOLOGY
(2021)
Article
Chemistry, Physical
Baofu Xu, Zining Li, Tyler A. Alsup, Michelle A. Ehrenberger, Jeffrey D. Rudolf
Summary: This study reveals the ability of terpene synthases to catalyze prenylation on small molecules, supports its physiological relevance in vivo, and proposes a substrate decoy mechanism.
Article
Biochemistry & Molecular Biology
Sachin Dhawale, Madhuri Pandit, Kanchan Thete, Dnyaneshwari Ighe, Sachin Gawale, Pallavi Bhosle, Deepak K. Lokwani
Summary: Candida albicans is a common fungus that can cause life-threatening infections and high mortality rates. In this study, an in silico approach was used to analyze the inhibitory potential of polyphenols from plants against a crucial enzyme in the cell wall of C. albicans.
JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS
(2023)
Review
Biochemistry & Molecular Biology
Joanna Stefaniak, Michal G. Nowak, Marek Wojciechowski, Slawomir Milewski, Andrzej S. Skwarecki
Summary: Glucosamine-6-phosphate synthase (GlcN-6-P synthase) is an important target for the development of antimicrobial agents and antidiabetic drugs. Various compounds have been identified as inhibitors of this enzyme, but their antimicrobial activity is relatively low and they have poor penetration ability. The pro-drug approach has been used to improve the antimicrobial activity of these inhibitors. Additionally, heterocyclic and polycyclic compounds have shown antimicrobial activity and potential as inhibitors of the enzyme, pending confirmation of their selective toxicity.
JOURNAL OF ENZYME INHIBITION AND MEDICINAL CHEMISTRY
(2022)
Review
Immunology
Jorge A. Ortiz-Ramirez, Mayra Cuellar-Cruz, Everardo Lopez-Romero
Summary: The cell wall of fungi is complex and consists of various crystalline and amorphous polysaccharides. Many enzymes are involved in the synthesis and degradation of these polysaccharides, and they play important roles in cell structure and function. The cell wall is a potential target for antifungal drugs, as its constituents are different from those in humans. Chitin, beta-glucans, and cellulose are the most common polysaccharides in the fungal cell wall. The hexosamine biosynthesis pathway is critical for cell wall formation, and GlcN-6-P synthase is an enzyme that has been considered as a potential target for antifungals.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Tarequl Islam, Muhammad Fazle Rabbee, Jinhee Choi, Kwang-Hyun Baek
Summary: This review discusses the biosynthesis, regulatory factors, importance in the host, and abiotic factors affecting the production of bacilysin in Bacillus species.
Article
Microbiology
Xun Wang, N. Monford Paul Abishek, Heung Jin Jeon, Jin He, Heon M. M. Lim
Summary: sysRNA, as a regulatory tool, has lost popularity due to its unstable inhibition effect and low efficiency. However, it remains interesting for its easy design and low metabolic burden. In this study, we discovered a new method to identify RDT regions in vivo using sysRNA, which is important for addressing the challenge of rapid RNA degradation.
MICROBIOLOGY SPECTRUM
(2023)
Article
Biology
Hanqing Gu, Bi Lian, Yuxiang Yuan, Ci Kong, Yan Li, Chang Liu, Yijun Qi
Summary: This study identified the characteristics of 5' tsRNAs and 5' tRNA halves in Arabidopsis, revealing an important role of a 5' tsRNA in negatively regulating anti-fungal defense through association with AGO1.
SCIENCE CHINA-LIFE SCIENCES
(2022)
Article
Chemistry, Multidisciplinary
Isadora de Araujo Oliveira, Daniela Maria dos Santos Lucena, Bruno da Costa Rodrigues, Victoria Trindade Maller, Rodrigo Nunes da Fonseca, Diego Allonso, Adriane Regina Todeschini
Summary: Glutamine:fructose-6-phosphate amidotransferase (GFAT) is a key enzyme in the hexosamine biosynthetic pathway (HBP), which is important for the synthesis of amino sugars. GFAT also plays a significant role in cancer and diabetes. Over the past 60 years, there has been a significant increase in understanding the mechanisms and biological relevance of GFAT. This article explores the role of GFAT in health and disease and its potential as a target for antimicrobial and anticancer chemotherapy.
PURE AND APPLIED CHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Meetu Agarwal, Vijay Soni, Suresh Kumar, Biplab Singha, Vinay Kumar Nandicoori
Summary: The study demonstrates that the absence of the C-terminal extension region in Mycobacterium tuberculosis GlmU (GlmU(Mt)) abolishes its ability to complement its function, and orthologs from other bacteria also cannot compensate for its function. Point mutants of GlmU(Mt) can successfully restore its biological function by forming heterotrimers.
BIOCHEMICAL JOURNAL
(2021)
Article
Biochemistry & Molecular Biology
Chen Dong, Mei Zhang, Shanshan Song, Fang Wei, Lili Qin, Puqing Fan, Yongchun Shi, Xiaoran Wang, Ran Wang
Summary: As an essential antioxidant, carotenoids play a crucial role in the light harvesting for photosynthesis and defense against high light stress in plants. In this study, researchers identified a small subunit (SSU) of geranylgeranyl diphosphate synthase (GGPPS), NtSSU II, in Nicotiana tabacum. NtSSU II forms enzymatic components with NtGGPPS1 and NtPSY1 to regulate carotenoid biosynthesis. The study also found that NtSSU II is distributed in various plant tissues and is influenced by light conditions.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Entomology
Jian-Chun Song, Zhan-Jun Lu, Long Yi, Hai-Zhong Yu
Summary: Trehalose-6-phosphate synthase (TPS) is a key enzyme in regulating trehalose content in insects. In this study, a TPS gene named DcTPS1 was identified in Diaphorina citri, and its knockdown led to abnormal phenotype, increased mortality and malformation rate with decreased molting rate. Silencing of DcTPS1 significantly inhibited chitin metabolism and fatty acid metabolism in D. citri, with upregulated DEGs mainly involved in oxidative phosphorylation and downregulated DEGs mainly attributed to lysosome and ribosome processes. These results suggest that DcTPS1 plays a crucial role in the growth and development of D. citri.
Article
Chemistry, Medicinal
Aditya Shimoga Janakirama Rao, Venugopal Thegur Mudduraj Urs, Jayanna Nayakarahatty Devanna, Paramesha Mahadevappa, Ramesh Chapeyil Kumaran
Summary: The study evaluated the antibacterial effects of three bioactive compounds isolated from the leaves of two species of the genus Morus against selective pathogens, with cathafuran-B showing significant bacteriostatic and bactericidal effects. In-silico analysis suggested that cathafuran-B could potentially serve as a glucosamine-6-phosphate synthase inhibitor, opening up new prospects for further research in phytomedicine.
LETTERS IN DRUG DESIGN & DISCOVERY
(2021)
Article
Multidisciplinary Sciences
Muhammad Shakeel, Juan Du, Shang-Wei Li, Yuan-Jin Zhou, Naeem Sarwar, Xiaolan Guo
Summary: Parental RNAi (pRNAi) was studied in Cnaphalocrocis medinalis to silence the CmGNA gene. The results showed that pRNAi effectively reduced ovulation and hatched eggs per female, altered sex ratios, increased larval and pupal mortality, and decreased mRNA expression levels. These findings confirmed the potential role of pRNAi in insect pest resistance management strategies.
SCIENTIFIC REPORTS
(2022)
Article
Oncology
Yong Feng, Na Li, Yi Ren
Summary: The study revealed that GNPNAT1 is upregulated in non-small cell lung cancer (NSCLC) and is associated with poor prognosis. Overexpression of GNPNAT1 promotes the growth and metastasis of lung cancer cells, while knockdown of GNPNAT1 has the opposite effect. GNPNAT1 levels are positively correlated with histological type and pathological stage, and its upregulation is partly due to aberrant RNF2 expression.
CANCER MANAGEMENT AND RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Markus Moerk-Moerkenstein, Ralf Heermann, Yvonne Goepel, Kirsten Jung, Boris Goerke
MOLECULAR MICROBIOLOGY
(2017)
Article
Biochemistry & Molecular Biology
Grecia M. Gonzalez, Svetlana Durica-Mitic, Steven W. Hardwick, Martin C. Moncrieffe, Marcus Resch, Piotr Neumann, Ralf Ficner, Boris Goerke, Ben F. Luisi
NUCLEIC ACIDS RESEARCH
(2017)
Review
Microbiology
Petra Dersch, Muna A. Khan, Sabrina Muehlen, Boris Goerke
FRONTIERS IN MICROBIOLOGY
(2017)
Article
Biochemistry & Molecular Biology
Achim Dickmanns, Christopher P. Zschiedrich, Johannes Arens, Iwan Parfentev, Jan Gundlach, Romina Hofele, Piotr Neumann, Henning Urlaub, Boris Goerke, Ralf Ficner, Joerg Stuelke
JOURNAL OF BIOLOGICAL CHEMISTRY
(2018)
Article
Genetics & Heredity
Yvonne Goepel, Boris Goerke
Article
Biochemistry & Molecular Biology
Svetlana Durica-Mitic, Boris Goerke
Article
Biochemistry & Molecular Biology
Muna A. Khan, Svetlana Durica-Mitic, Yvonne Goepel, Ralf Heermann, Boris Goerke
Article
Biochemistry & Molecular Biology
Svetlana Durica-Mitic, Yvonne Goepel, Fabian Amman, Boris Goerke
Article
Biochemistry & Molecular Biology
Md Saiful Islam, Steven W. Hardwick, Laura Quell, Svetlana Durica-Mitic, Dimitri Y. Chirgadze, Boris Goerke, Ben F. Luisi
Summary: This article reveals the intricate post-transcriptional networks mediated by GlmZ in the biogenesis of GlcN6P, an essential precursor of the bacterial cell envelope. The cryoEM structure of the RapZ:GlmZ complex is presented, showing a complementary match between the RapZ tetrameric quaternary structure and the structural repeats in the sRNA. The structure of a precleavage intermediate formed between the binary RapZ:GlmZ complex and RNase E is also presented, illustrating how GlmZ is recognized by the enzyme.
Article
Biochemistry & Molecular Biology
Alexandra Schilder, Boris Goerke
Summary: In enteric bacteria, small RNAs (sRNAs) such as MicC stimulate target RNA decay using RNase E. The interaction between the sRNA 5' monophosphate (5 ' P) and RNase E activates cleavage of the target. However, experimental evidence supporting this model is limited. To address this, researchers developed a genetic tool to generate controllable 5' monophosphorylated sRNAs. The results showed that while the 5' phosphorylation state determines stability of naturally triphosphorylated sRNAs, it does not play a major role in target RNA destabilization in vivo. Additionally, the C-terminal half of RNase E is crucial for MicC-mediated decay, suggesting the mandatory interaction with Hfq.
NUCLEIC ACIDS RESEARCH
(2023)
Article
Biochemistry & Molecular Biology
Kornelia Skarbek, Iwona Gabriel, Piotr Szweda, Marek Wojciechowski, Muna A. Khan, Boris Goerke, Slawomir Milewski, Maria J. Milewska
CARBOHYDRATE RESEARCH
(2017)
Article
Microbiology
Svetlana Durica-Mitic, Yvonne Goepel, Boris Goerke
MICROBIOLOGY SPECTRUM
(2018)