期刊
REVIEWS IN THE NEUROSCIENCES
卷 22, 期 1, 页码 121-130出版社
WALTER DE GRUYTER GMBH
DOI: 10.1515/RNS.2011.013
关键词
neurobiology; neuronal circuits; optogenetics; zebrafish
资金
- CNRS (ATIP: Action Thematique et Incitative sur Programme)/INSERM
- Marie Curie Outgoing International Fellowship (with the laboratory Institut Curie CNRS - UMR3215 'Unite de Genetique et Biologie du Developpement', Paris, France)
Optogenetics has revolutionized the toolbox arsenal that neuroscientists now possess to investigate neuronal circuit function in intact and living animals. With a combination of light emitting 'sensors' and light activated 'actuators', we can monitor and control neuronal activity with minimal perturbation and unprecedented spatiotemporal resolution. Zebrafish neuronal circuits represent an ideal system to apply an optogenetic based analysis owing to its transparency, relatively small size and amenability to genetic manipulation. In this review, we describe some of the most recent advances in the development and applications of optogenetic sensors (i.e., genetically encoded calcium indicators and voltage sensors) and actuators (i.e., light activated ion channels and ion pumps). We focus mostly on the tools that have already been successfully applied in zebrafish and on those that show the greatest potential for the future. We also describe crucial technical aspects to implement optogenetics in zebrafish including strategies to drive a high level of transgene expression in defined neuronal populations, and recent optical advances that allow the precise spatiotemporal control of sample illumination.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据