Review
Biochemistry & Molecular Biology
Jongmin Kim, Yeo Jin Lee, Jae Yon Won
Summary: This review highlights the roles and pathogenesis of retinal pigment epithelium (RPE) in AMD, emphasizing the importance of understanding the etiology of AMD and discussing potential treatment strategies targeting RPE.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Multidisciplinary Sciences
Masahiro Miura, Shuichi Makita, Yoshiaki Yasuno, Takuya Iwasaki, Shinnosuke Azuma, Toshihiro Mino, Tatsuo Yamaguchi
Summary: The study quantitatively evaluated retinal pigment epithelium changes in patients with age-related macular degeneration using prototype multi-contrast optical coherence tomography. The results showed that multi-contrast OCT is useful for objective evaluation of changes in the retinal pigment epithelium.
SCIENTIFIC REPORTS
(2021)
Article
Biochemistry & Molecular Biology
Cody R. Fisher, Mara C. Ebeling, Zhaohui Geng, Rebecca J. Kapphahn, Heidi Roehrich, Sandra R. Montezuma, James R. Dutton, Deborah A. Ferrington
Summary: This study evaluated the treatment response of haRPE and iPSC-RPE to oxidative stress and potential therapeutics for mitochondrial defects. The results showed disease-specific responses in haRPE, while iPSC-RPE had similar responses regardless of disease status. Analysis of cells generated from the same donor showed a similar response for donors without AMD, but significant differences in treatment response between cell models from AMD donors.
Article
Chemistry, Physical
Zhimin Tang, Minfeng Huo, Yahan Ju, Xiaochan Dai, Ni Ni, Yan Liu, Huiqin Gao, Dandan Zhang, Hao Sun, Xianqun Fan, Yu Chen, Ping Gu
Summary: The iron-binding nanoscale Prussian blue analogue CaPB effectively prevents RPE degeneration and subsequent photoreceptor cell degeneration, showing superior therapeutic outcomes in mice upon a single intravitreal injection. The findings suggest that CaPB nanoparticles protect against RPE degradation by inhibiting ferroptotic cell fate, making them promising for future clinical treatment of retinal diseases involving iron-dependent ferroptosis.
Article
Cell Biology
Ha Young Jang, Chang Sik Cho, Young Mi Shin, Jina Kwak, Young Hoon Sung, Byeong-Cheol Kang, Jeong Hun Kim
Summary: This study investigated the mechanisms underlying retinal pigment epithelium dysfunction in aging and age-related macular degeneration (AMD) using primary marmoset cells. Comparisons were made between the effects of fetal bovine serum (FBS) and serum-free supplements on cell characteristics, and age-related morphological changes were assessed. The results suggest that primary marmoset RPE cells provide a reliable in vitro model for developing therapeutics for AMD.
Article
Biochemistry & Molecular Biology
Satoru Ando, Noriyasu Hashida, Daisuke Yamashita, Tsuyoshi Kawabata, Kazunobu Asao, Satoshi Kawasaki, Kazushi Sakurai, Tamotsu Yoshimori, Kohji Nishida
Summary: The study reveals that toxic fluorophore A2E impairs autophagy and upregulates Rubicon in RPE. Treatment with Rubicon-specific siRNA can reverse this impairment, and RPE-specific Rubicon deletion plays a positive role in alleviating retinal inflammation reactions.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2021)
Article
Biochemistry & Molecular Biology
Sofia Ranta-aho, Niina Piippo, Eveliina Korhonen, Kai Kaarniranta, Maria Hytti, Anu Kauppinen
Summary: The study found that TAS-116 could prevent the activation of caspase-1, subsequently reducing the release of mature IL-1 beta. TAS-116 has a better in vitro therapeutic index than geldanamycin. In summary, TAS-116 appears to be a well-tolerated Hsp90 inhibitor, with the capability to prevent the activation of the NLRP3 inflammasome in human RPE cells.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Editorial Material
Neurosciences
Roberto Pinelli, Michela Ferrucci, Francesca Biagioni, Violet Bumah, Elena Scaffidi, Stefano Puglisi-Allegra, Francesco Fornai
Summary: Defective autophagy in the RPE is involved in AMD, and curcumin can protect RPE cells from damage induced by the autophagy inhibitor 3-MA by activating autophagy.
CURRENT NEUROPHARMACOLOGY
(2023)
Article
Medicine, General & Internal
Junya Nagata, Satomi Shiose, Keijiro Ishikawa, Takuma Fukui, Kumiko Kano, Kenichiro Mori, Takahito Nakama, Shoji Notomi, Koh-Hei Sonoda, Tsutomu Yasukawa
Summary: This study retrospectively analyzed the clinical characteristics of RPE tears in nAMD patients and found that RPE tears were more common in typical AMD eyes with high or large PEDs and sub-RPE clefts. The visual prognosis depended on whether the RPE tear involved the fovea.
JOURNAL OF CLINICAL MEDICINE
(2023)
Article
Medicine, General & Internal
Hyunchae Sim, Wonhwa Lee, Samyeol Choo, Eui Kyun Park, Moon-Chang Baek, In-Kyu Lee, Dong Ho Park, Jong-Sup Bae
Summary: A study evaluated the protective effects of SFN from cruciferous vegetables against PM2.5-induced oxidative damage, showing that SFN effectively alleviates oxidative damage in human ARPE-19 cells via its antioxidant effects.
FRONTIERS IN MEDICINE
(2021)
Review
Biochemistry & Molecular Biology
Santosh Gupta, Lyubomyr Lytvynchuk, Taras Ardan, Hana Studenovska, Georgina Faura, Lars Eide, Ljubo Znaor, Slaven Erceg, Knut Stieger, Jan Motlik, Kapil Bharti, Goran Petrovski
Summary: The retinal pigment epithelium (RPE) plays a crucial role in maintaining the normal physiology of the eye, and damage to the RPE can lead to degenerative eye diseases. Currently, stem cell-derived RPE transplantation is being explored for treating early stages of age-related macular degeneration (AMD). This article focuses on developmental studies and stem cell-based research to understand the biology and derive functional RPEs.
Article
Cell Biology
Mara C. Ebeling, Cody R. Fisher, Rebecca J. Kapphahn, Madilyn R. Stahl, Shichen Shen, Jun Qu, Sandra R. Montezuma, Deborah A. Ferrington
Summary: This study found that the inflammasome is present and active in RPE, but it may not contribute to the pathology of early AMD.
Article
Cell Biology
Wenchang Xu, Xinqi Liu, Wenjuan Han, Keling Wu, Minglei Zhao, Tingfang Mei, Bizhi Shang, Jinwen Wu, Jingyi Luo, Yuhua Lai, Boyu Yang, Yehong Zhuo, Lin Lu, Yizhi Liu, Xiao-li Tian, Ling Zhao
Summary: The overexpression of HTRA1 promotes cellular senescence in RPE, damaging mitochondrial function and activating HIF-1 signaling, which contributes to the pathogenesis of AMD. Inhibition of HIF-1 signaling may serve as a potential therapeutic strategy for AMD.
CELL COMMUNICATION AND SIGNALING
(2023)
Article
Multidisciplinary Sciences
Brandi L. Williams, Nathan A. Seager, Jamie D. Gardiner, Chris M. Pappas, Monica C. Cronin, Cristina Amat di San Filippo, Robert A. Anstadt, Jin Liu, Marc A. Toso, Lisa Nichols, Timothy J. Parnell, Jacqueline R. Eve, Paul L. Bartel, Moussa A. Zouache, Burt T. Richards, Gregory S. Hageman
Summary: Research has shown that the HTRA1 gene in the 10q26 locus is downregulated in patients with age-related macular degeneration (AMD) and this is mediated by a noncoding, cis-regulatory element overlapping the ARMS2 intron, potentially leading to an increased risk of disease.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Multidisciplinary Sciences
Pei-Li Yao, Vipul M. Parmar, Mayur Choudhary, Goldis Malek
Summary: The expression and activity of NURR1 play critical roles in the physiological and pathological functions of human RPE cells. Targeting NURR1 may have therapeutic potential for AMD by improving EMT, inflammation, and lipid homeostasis in RPE cells.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Biochemistry & Molecular Biology
Anson Snow, Biehuoy Shieh, Kun-Che Chang, Arttatrana Pal, Patricia Lenhart, David Ammar, Philip Ruzycki, Suryanarayana Palla, G. Bhanuprakesh Reddy, J. Mark Petrash
CHEMICO-BIOLOGICAL INTERACTIONS
(2015)
Article
Multidisciplinary Sciences
Niklaus H. Mueller, Uma Fogueri, Michelle G. Pedler, Kameron Montana, J. Mark Petrash, David A. Ammar
Article
Ophthalmology
Jeffrey R. SooHoo, Leonard K. Seibold, David A. Ammar, Malik Y. Kahook
JOURNAL OF OPHTHALMOLOGY
(2015)
Review
Biochemistry & Molecular Biology
Ram H. Nagaraj, Rooban B. Nahomi, Niklaus H. Mueller, Cibin T. Raghavan, David A. Ammar, J. Mark Petrash
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS
(2016)
Article
Ophthalmology
Dinesh G. Goswami, Neera Tewari-Singh, Deepanshi Dhar, Dileep Kumar, Chapla Agarwal, David A. Ammar, Rama Kant, Robert W. Enzenauer, J. Mark Petrash, Rajesh Agarwal
Article
Ophthalmology
Neera Tewari-Singh, Claire R. Croutch, Richard Tuttle, Dinesh G. Goswami, Rama Kant, Eric Peters, Tara Culley, David A. Ammar, Robert W. Enzenauer, J. Mark Petrash, Robert P. Casillas, Rajesh Agarwal
CUTANEOUS AND OCULAR TOXICOLOGY
(2016)
Article
Toxicology
Neera Tewari-Singh, Dinesh G. Goswami, Rama Kant, David A. Ammar, Dileep Kumar, Robert W. Enzenauer, Robert P. Casillas, Claire R. Croutch, J. Mark Petrash, Rajesh Agarwal
TOXICOLOGICAL SCIENCES
(2017)
Article
Biochemistry & Molecular Biology
Karen L. Christopher, Michelle G. Pedler, Biehuoy Shieh, David A. Ammar, J. Mark Petrash, Niklaus H. Mueller
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2014)
Article
Veterinary Sciences
Alexis Dubin, Kate S. Freeman, Joseph Charles, David A. Ammar, Eugene J. Ehrhart
Summary: This study demonstrated the presence of lymphatics in the canine anterior uvea, potentially indicating a novel uveolymphatic outflow pathway that may play a role in aqueous humor outflow. Further research is needed to confirm the existence and elucidate the role of this proposed uveolymphatic outflow pathway and potentially develop novel treatment options for managing glaucoma.
VETERINARY OPHTHALMOLOGY
(2021)
Article
Toxicology
Satyendra K. Singh, Dinesh G. Goswami, Holly N. Wright, Rama Kant, Izza A. Ali, Leah N. Braucher, Joshua A. Klein, Madeline G. Godziela, David A. Ammar, Kathryn M. Pate, Neera Tewari-Singh
Summary: With the potential use of chemical weapons in warfare or terrorist activities, effective therapies for ocular injuries caused by exposure are critical. Oxygen plays a key role in preserving ocular tissue and promoting wound repair. The study evaluated the efficacy of supersaturated oxygen emulsion (SSOE) in reducing corneal and keratocyte injury from chloropicrin (CP), a chemical threat agent. The results showed that SSOE treatment had a healing effect on corneal epithelial layer and reduced CP-induced cell death and DNA damage. Further research on SSOE's treatment efficacy in in vivo corneal injury models for CP and vesicants is warranted.
TOXICOLOGY LETTERS
(2021)
Article
Ophthalmology
Enrico Brambilla, Tanner J. Ferguson, Nathan Chu, David Ammar, Paul Yoo
Summary: This study investigates the agreement between pneumatonometry and direct pressure transducer intraocular pressure (IOP) measurements. The results show that IOP measured using excursion tonometry is within the accuracy range of the pneumatonometry device and is minimally impacted by negative pressure (NP) application.
CLINICAL OPHTHALMOLOGY
(2022)
Article
Ophthalmology
Leonid M. Zukin, Michelle G. Pedler, Sergio Groman-Lupa, Mina Pantcheva, David A. Ammar, J. Mark Petrash
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
(2018)
Article
Ophthalmology
Xianzeng Zhang, Nenrong Liu, Peng Un Mak, Sio Hang Pun, Mang I. Vai, Omid Masihzadeh, Malik Y. Kahook, Tim C. Lei, David A. Ammar
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
(2016)
Article
Ophthalmology
Tim C. Lei, Omid Masihzadeh, Malik Y. Kahook, David A. Ammar
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
(2013)
Article
Ophthalmology
David A. Ammar, Leonard K. Seibold, Malik Y. Kahook
CLINICAL OPHTHALMOLOGY
(2020)