Article
Biology
Jonathan D. Hibshman, Serena Carra, Bob Goldstein
Summary: Small heat shock proteins (sHSPs) play important roles in heat tolerance, but their potential roles in desiccation tolerance have not been thoroughly explored. This study identified specific sHSPs from the tardigrade Hypsibius exemplaris that can improve desiccation tolerance in E. coli, suggesting a conserved mechanism for desiccation protection. Furthermore, the study found that two tardigrade sHSPs, HSP21 and HSP24.6, can oligomerize and prevent protein aggregation, promoting heat and desiccation tolerance.
COMMUNICATIONS BIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Jinhee Park, Samantha MacGavin, Laurie Niederbrach, Hassane S. Mchaourab
Summary: A coordinated oxidative stress response, partly triggered by the transcription factor Nrf2, protects cells from harmful reactive oxygen species. The interplay between oxidative stress and chaperone responses in the lens and heart remains unexplored.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2023)
Article
Cell Biology
Yuan Xiao, Jia-Wen Xiang, Qian Gao, Yue-Yue Bai, Zhao-Xia Huang, Xiao-Hui Hu, Ling Wang, David Wan-Cheng Li
Summary: Research has shown that Mab21L1 promotes survival of lens epithelial cells by regulating the expression of αB-crystallin and suppressing the ATR/CHK1/p53 pathway. This finding contributes to a better understanding of the functional mechanism of Mab21L1.
Review
Biochemistry & Molecular Biology
Roy A. Quinlan, John I. Clark
Summary: In this review, the authors highlight the potential protective effects of post-translational modifications (PTMs) on lens proteins during normal development and propose a forward-looking hypothesis that aims to prevent age-related cataract (ARC) by protecting the biochemical and biophysical properties of lens proteins.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2022)
Article
Chemistry, Multidisciplinary
Giovanni Palomino-Vizcaino, Nils Schuth, Jose A. Dominguez-Calva, Oscar Rodriguez-Meza, Eduardo Martinez-Jurado, Eugene Serebryany, Jonathan A. King, Thomas Kroll, Miguel Costas, Liliana Quintanar
Summary: Cataracts are caused by the aggregation of high-molecular-weight proteins in the human eye lens, which scatter light and cause lens opacity. Metal ions play an important role in the development of cataract disease, as human lens gamma-crystallins are susceptible to metal-induced aggregation.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2023)
Article
Biology
Manju Nidagodu Jayakumar, Jibran Sualeh Muhammad, Mainak Dutta, Sainitin Donakonda
Summary: This study aims to identify and understand the involvement of chaperones in the pathogenesis of cholangiocarcinoma (CCA), a subtype of liver cancer. Through literature mining, expression analysis, and pathway analysis, the researchers identified novel therapeutic targets (CRYAB and P4HA2) and potential inhibitors (tucatinib and regorafenib) for CCA. The study provides new insights into the pan-chaperone expression in CCA and offers potential alternative approaches to its treatment.
COMPUTERS IN BIOLOGY AND MEDICINE
(2023)
Article
Multidisciplinary Sciences
Klaus Hojgaard Jensen, Anna Katharina Stalder, Rasmus Wernersson, Tim-Christoph Roloff-Handschin, Daniel Hvidberg Hansen, Peter M. A. Groenen
Summary: This study investigated the molecular biology of Amyotrophic Lateral Sclerosis (ALS) using protein-protein interaction networks and identified a set of core modules enriched in ALS-associated proteins. These core modules not only captured most of the current knowledge about ALS, but also revealed biological interdependencies and associations with other neurodegenerative diseases. The presence of recently identified ALS-associated proteins in the core modules suggests their potential for identifying novel ALS disease mechanisms.
Article
Physiology
Jiayue Zhou, Jing Wu, Sifan Zheng, Xiangjun Chen, Daizhan Zhou, Xingchao Shentu
Summary: Cold cataract refers to the reversible opacification of the lens as temperature decreases, but prolonged exposure to low temperature can lead to irreversible opacification. Transcriptomic analysis showed up-regulation of genes related to lens structure and development, with small heat shock proteins identified as hub genes. Proteomic analysis revealed up-regulation of the apoptotic process, and integrated analysis suggested down-regulation of specific signaling pathways as part of the regulatory mechanism in the irreversible opacification process.
FRONTIERS IN PHYSIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Junna Hayashi, Jennifer Ton, Sparsh Negi, Daniel E. K. M. Stephens, Dean L. Pountney, Thomas Preiss, John A. Carver
Summary: Oxidation of dopamine is a pathological feature of Parkinson's disease, leading to the formation of adducts with proteins. The small heat-shock molecular chaperone proteins, alpha B-crystallin and Hsp27, are involved in preventing protein aggregation under cellular stress. When oxidized by dopamine, these proteins showed increased oligomeric size without significant alteration in secondary structure, maintaining chaperone functionality. Higher levels of oxidation reduced chaperone effectiveness in preventing protein aggregation associated with PD.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Binyou Wang, Stuart P. Moon, Giuliano Cutolo, Afraah Javed, Benjamin S. Ahn, Andrew H. Ryu, Matthew R. Pratt
Summary: O-GlcNAc modification protects cells against stress-induced cell death by directly inhibiting the apoptotic pathway. The modified chaperone, HSP27, exhibits enhanced antiapoptotic function in an in vitro context. These findings provide molecular insights into how O-GlcNAc serves as a mediator of cellular stress and have important implications for human diseases like cancer and neurodegeneration.
ACS CHEMICAL BIOLOGY
(2023)
Article
Oncology
Shenghao Wu, Yuemiao Chen, Xueshuang Wang, Shanshan Weng, Wenjin Zhou, Zhen Liu
Summary: This study found that EPA can inhibit cell proliferation, induce apoptosis, and regulate Hsp90 and GR alpha protein expression, potentially offering a new effective treatment for reversal of glucocorticoid-resistance in multiple myeloma.
Article
Biochemistry & Molecular Biology
Saad M. Ahsan, Raman Bakthisaran, Ramakrishna Tangirala, Ch Mohan Rao
Summary: This study reveals that alterations in the interactome and nucleosomal association, rather than loss of chaperone-like activity, are the molecular basis of cataract caused by the R54C mutation in alpha A-crystallin. Furthermore, it provides a novel mechanism of cataract caused by this mutant and sheds light on the possible mechanisms of stress and cell death induced by nuclear inclusions.
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS
(2021)
Review
Chemistry, Physical
Marc A. Sprague-Piercy, Megan A. Rocha, Ashley O. Kwok, Rachel W. Martin
Summary: Alpha-crystallins are small heat-shock proteins acting as holdase chaperones with the ability to prevent aggregation of damaged crystallins. They form dynamic, heterogeneous oligomers with central domains flanked by flexible extensions, and control oligomerization through domain swapping. Mutations or modifications can compromise their chaperone activity, leading to protein aggregation and cataract formation.
ANNUAL REVIEW OF PHYSICAL CHEMISTRY, VOL 72
(2021)
Review
Cell Biology
Tong Lei, Zhuangzhuang Xiao, Wangyu Bi, Shanglin Cai, Yanjie Yang, Hongwu Du
Summary: Neurodegenerative diseases are aging-related diseases involving neuron death. Small heat shock proteins play a crucial role in maintaining cellular homeostasis and resisting toxic protein aggregation. Research suggests that enhancing HSPB expression and delivery can improve manifestations of neurodegenerative diseases.
AGEING RESEARCH REVIEWS
(2022)
Article
Multidisciplinary Sciences
Tsukumi Miwa, Hideki Taguchi
Summary: Research has shown that IbpA down-regulates the expression of multiple heat shock proteins (Hsps) by repressing the translation of the heat shock transcription factor sigma(32), and plays a crucial role in shutting down the heat shock response. These findings reveal an unknown role of IbpA in regulating the heat shock response at the translational level.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Ophthalmology
D. Claire Miller, Karen L. Christopher, Jennifer L. Patnaik, Anne M. Lynch, Leonard K. Seibold, Naresh Mandava, Michael J. Taravella
Summary: The study found that patients who received intravitreal anti-vascular endothelial growth factor injections prior to cataract surgery were at a higher risk of posterior capsule rupture. This risk was particularly high for males and diabetic patients. Additionally, the presence of mature cataracts and having a resident as the primary surgeon were also associated with an increased risk of PCR during surgery.
CURRENT EYE RESEARCH
(2021)
Article
Ophthalmology
D. Claire Miller, Jennifer L. Patnaik, Alan G. Palestine, Anne M. Lynch, Karen L. Christopher
Summary: This study compared cataract surgery complications and visual outcomes in patients with and without HIV, finding that HIV-positive patients were at greater risk of developing persistent anterior uveitis and clinically significant cystoid macular edema after surgery. However, HIV-positive patients had similar best corrected distance visual acuity after cataract surgery compared to HIV-negative patients.
OPHTHALMIC EPIDEMIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Justin E. Fichtner, Jennifer Patnaik, Karen L. Christopher, J. Mark Petrash
Summary: The study surveyed cataract surgeons' perspectives on preventative PCO therapy, finding that the majority believe there is a need for such treatment and are interested in incorporating it into surgical protocols. They perceive benefits including optimizing visual outcomes, eliminating complications, and providing solutions for specific patient groups.
CHEMICO-BIOLOGICAL INTERACTIONS
(2021)
Letter
Ophthalmology
Karen L. Christopher, Cristos Ifantides, Anne M. Lynch, Jennifer L. Patnaik
JOURNAL OF CATARACT AND REFRACTIVE SURGERY
(2022)
Article
Multidisciplinary Sciences
Michelle G. Pedler, J. Mark Petrash, Prem S. Subramanian
Summary: Brain cerebrospinal fluid outflow can be increased by nasal inhalation of ophthalmic prostaglandin F2alpha analogs, with the greatest effect observed with latanoprost. Further studies are required to assess the efficacy of these agents in reducing intracranial pressure in both short and long-term applications.
Article
Ophthalmology
Ashton Kalhorn, Jennifer L. Patnaik, Cristos Ifantides, Cara E. Capitena Young, Anne M. Lynch, Karen L. Christopher
Summary: The study aimed to investigate the relationship between surgical duration and post-operative outcomes in patients undergoing simple cataract surgery. The results showed that long surgeries were associated with an increased need for Nd:YAG capsulotomy and poorer post-operative visual acuity. However, there was no association between prolonged postoperative inflammation and long surgeries.
OPHTHALMIC EPIDEMIOLOGY
(2023)
Article
Ophthalmology
Enrico Brambilla, Tanner J. Ferguson, Nathan Chu, David Ammar, Paul Yoo
Summary: This study investigates the agreement between pneumatonometry and direct pressure transducer intraocular pressure (IOP) measurements. The results show that IOP measured using excursion tonometry is within the accuracy range of the pneumatonometry device and is minimally impacted by negative pressure (NP) application.
CLINICAL OPHTHALMOLOGY
(2022)
Article
Ophthalmology
Karen L. Christopher, Jennifer L. Patnaik, Kylie J. Penland, Mina B. Pantcheva, Anne M. Lynch, Cristos Ifantides
Summary: This study investigated the outcomes of cataract surgery in patients with HCV seropositivity and explored risk factors for surgical complications. The results showed that HCV positive patients were more likely to experience complications during cataract surgery. However, regardless of HCV status, the final best corrected visual acuity was excellent.
OPHTHALMIC EPIDEMIOLOGY
(2023)
Article
Ophthalmology
Karen L. Christopher, Staci Terrin, John Lohmeier, Matthew Hartman, Richard S. Davidson, Michael J. Taravella
Summary: The aim of this study was to compare endothelial cell loss for DMEK tissue preparation techniques using the modified Jones tube and the DMEK EndoGlide with and without viscoelastic material to protect the endothelium. The results showed that both the Jones tube and DMEK EndoGlide resulted in similar low rates of endothelial cell loss after tissue preparation, transport, and deployment. However, the use of viscoelastic material to protect the endothelium using the DMEK technique actually resulted in increased cell loss in the area of its application, resulting in overall higher rates of cell loss across the DMEK tissue.
Article
Ophthalmology
Julia L. Xia, Jennifer L. Patnaik, Anne M. Lynch, Karen L. Christopher
Summary: This study compared outcomes of cataract surgery in patients with type 1 diabetes mellitus (T1DM), type 2 diabetes mellitus (T2DM), and without diabetes mellitus (DM). The results showed that T1DM patients had worse visual outcomes after surgery, which were associated with preoperative diabetic retinopathy and retinal detachment history, rather than the type of diabetes.
JOURNAL OF CATARACT AND REFRACTIVE SURGERY
(2023)
Letter
Ophthalmology
Karen L. Christopher, Jennifer L. Patnaik, Anne M. Lynch, Nathan Grove
CLINICAL AND EXPERIMENTAL OPHTHALMOLOGY
(2023)
Article
Ophthalmology
Sophie E. Smith, Anne M. Lynch, Emily A. Auer, Kirk A. Bol, Karen L. Christopher, Naresh Mandava, Jennifer L. Patnaik
Summary: This study aimed to investigate the relationship between visual functioning as measured by the National Eye Institute 25-Item Visual Function Questionnaire (VFQ-25) and mortality in patients with age-related macular degeneration (AMD). The results showed that better general health and ability to drive were each separately associated with significantly lower risk of death among individuals with AMD.
OPHTHALMOLOGY RETINA
(2023)
Article
Ophthalmology
Cristos Ifantides, Steven M. Naids, Danson Muttuvelu, Shahzad Mian, Karen L. Christopher
Summary: The Yamane intrascleral flanged haptic fixation technique eliminates the need for resources like suture or glue, but externalizing the haptics can be challenging for eye surgeons, especially when visualization through the cornea is poor. The modified technique described in the study docks the second haptic externally at the main corneal incision, avoiding the use of microforceps and potentially offering increased safety and ease for surgeons.
CLINICAL OPHTHALMOLOGY
(2021)
Article
Ophthalmology
Karen L. Christopher, Jennifer L. Patnaik, Cristos Ifantides, D. Claire Miller, Richard S. Davidson, Michael J. Taravella, Anne Lynch, Brandie Wagner
Summary: This retrospective study evaluated the time cost and prediction error of intraoperative aberrometry (IA) compared to conventional preoperative calculations. Results showed that for eyes with extremes in axial length, prior myopic corneal refractive surgery, or implanting lenses with certain properties, IA provided a clinically meaningful improvement in outcomes despite added surgical time. Additionally, the study identified strong predictors for eyes that benefited most from IA, such as prior myopic corneal refractive surgery and specific lens implantations.
CLINICAL OPHTHALMOLOGY
(2021)
Article
Biochemistry & Molecular Biology
G. F. Senguel, R. Mishra, E. Candiello, P. Schu
Summary: AP2 forms AP2 CCV with clathrin and other coat proteins, and synapses contain different types of CCV. The stability and composition of CCV are regulated by various factors, including Hsc70 and phosphorylation patterns. The knockout of the AP1/O1B complex disrupts synaptic vesicle recycling and endosomal protein sorting, leading to upregulation of endocytosis. Stable CCV, termed stCCV, have distinct characteristics and specialized functions in synaptic plasticity. The phosphorylation of Hsc70 and the levels of kinases play a crucial role in regulating the stability and disassembly of clathrin in CCV.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Martin Fluck, Colline Sanchez, Vincent Jacquemond, Christine Berthier, Marie-Noelle Giraud, Daniel Jacko, Kathe Bersiner, Sebastian Gehlert, Guus Baan, Richard T. Jaspers
Summary: Enhancing CaMKII signaling improves fatigue resistance and contractile characteristics of skeletal muscle by enhancing calcium release.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Letter
Biochemistry & Molecular Biology
Federica Coppola, Sara Monaci, Alessandro Falsini, Carlo Aldinucci, Irene Filippi, Daniela Rossi, Fabio Carraro, Antonella Naldini
Summary: The adaptor protein p62 plays a crucial role in maintaining the survival of dendritic cells (DCs) under hypoxic conditions by preserving Erk1/2 phosphorylation and reducing AMPK activation, thus extending their lifespan to ensure their functions in hypoxic microenvironments.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Jenifer Pendiuk Goncalves, Jorvani Cruz Villarreal, Sierra A. Walker, Xuan Ning Sharon Tan, Chad Borges, Joy Wolfram
Summary: This study used a mass spectrometry-based approach to assess the differences in glycan features between extracellular vesicles (EVs) and originating cells. The results showed that EVs selectively enriched specific glycan features, particularly those associated with binding to the extracellular matrix. The study also found differences in EV glycan sorting between different metastatic cell lines and mouse models, indicating a potential role of glycan diversity in the metastatic process.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
De-ao Gong, Peng Zhou, Wen-yi Chang, Jia-yao Yang, Yan-lai Zhang, Ai-long Huang, Ni Tang, Kai Wang
Summary: Liver cancer, ranked sixth globally, is a major contributor to cancer-related mortality. Metastasis is the main cause of treatment failure and deaths in liver cancer. The SPOP-CREB5-MET axis plays a significant role in liver cancer metastasis.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Ning Huang, Jun Tang, Xiaoyao Yi, Maoxin Zhang, Bin Li, Yuan Cheng, Jin Chen
Summary: This study reveals that glioma-derived S100A9 can induce microglial M2 polarization, inhibit CD8+ T lymphocytes, and promote immunosuppression. The mechanism is related to the interaction with alpha v133 integrin and subsequent activation of AKT1 in microglia. The expression of S100A9 is positively associated with CD206 expression and negatively correlated with CD8+ T lymphocyte accumulation in the TME, suggesting a potential role of S100A9 in regulating the tumor microenvironment and immune evasion in glioma.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Yomna S. Abd El-Aziz, Matthew J. McKay, Mark P. Molloy, Betty McDowell, Elizabeth Moon, Loretta Sioson, Amy Sheen, Angela Chou, Anthony J. Gill, Patric J. Jansson, Sumit Sahni
Summary: This study identified a novel combination of autophagy inhibitors that can effectively inhibit the proliferation of oral squamous cell carcinoma (OSCC) cells, including both chemosensitive and chemoresistant cells. This research is important for the development of new therapies for advanced OSCC tumors.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Luojia Liu, Xiaoqiang Liu, Ying Chen, Meng Kong, Jinghong Zhang, Min Jiang, Hongling Zhou, Jinrui Yang, Xu Chen, Ze Zhang, Chao Wu, Xupin Jiang, Jiaping Zhang
Summary: Our study revealed that the Paxillin/HDAC6 signaling pathway regulates microtubule acetylation in electric field-guided keratinocyte migration.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Julia Weikum, Jeroen F. van Dyck, Saranya Subramani, David P. Klebl, Merete Storflor, Stephen P. Muench, Soren Abel, Frank Sobott, J. Preben Morth
Summary: The study reveals the complex interaction between bacterial magnesium transporter A (MgtA) and cardiolipin 18:1 and cardiolipin 16:0, highlighting the importance of lipid environment in protein activity and stability. Further understanding of Mg2+ homeostasis in bacteria will provide insights into bacterial infections.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Sumit Kinger, Yuvraj Anandrao Jagtap, Ankur Rakesh Dubey, Prashant Kumar, Akash Choudhary, Rohan Dhiman, Vijay Kumar Prajapati, Deepak Chitkara, Krishna Mohan Poluri, Amit Mishra
Summary: Efficient protein synthesis and quality control mechanisms are crucial for maintaining proteostasis and preventing neurodegeneration. This study demonstrates that treating cells with Lanosterol can enhance the proteolytic activity of Proteasome and promote the removal of misfolded proteins, suggesting a potential therapeutic approach for abnormal protein accumulation.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Karolina Stepien, Adrianna Skoneczna, Monika Kula-Maximenko, Lukasz Jurczyk, Mateusz Molon
Summary: The replication of DNA requires a complex machinery called the replisome, which is highly conserved across species. One crucial component of the replisome is the CMG helicase complex, which unwinds DNA and coordinates the assembly and function of other replisome components. In this study, the impact of the absence of one copy of the CMG complex genes on the physiology and aging of yeast cells was investigated. The findings showed disruptions in the cell cycle, extended doubling times, and alterations in the biochemical profile of these cells. Importantly, it was found that heterozygous cells for CMG helicase genes exhibited increased reproductive potential and delayed aging. The study also highlighted potential therapeutic targets for cancer treatment using yeast.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Nishadh Rathod, Guadalupe Guerrero-Serna, Howard S. Young, L. Michel Espinoza-Fonseca
Summary: This study reveals that replacing Lys27 with Asn enhances the inhibitory potency of MLN without affecting SERCA's affinity for Ca2+. The findings suggest that the SERCA site modulating Ca2+ affinity also functions as a catalytic activity switch.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Can Jiang, Chunyang Zhang, Min Dai, Fuyan Wang, Sa Xu, Dan Han, Yanyan Wang, Yajie Cao, Yanyan Liang, Ziyu Zhang, Lina Yan, Yujun Shen, Kewu He, Yuxian Shen, Jun Liu
Summary: The phosphorylation of p65 and the expression of SUMO1 are increased in cancer tissues of HCC patients, and there is a positive correlation between SUMO1 and phosphorylated p65. SUMOylation of p65 by SUMO1 promotes p65 nuclear import and enhances NF-xB activity. Both SUMOylation and phosphorylation of p65 increase the viability and invasion of hepatoma cells, and decrease cell apoptosis.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Ming-Fo Hsu, Yoshihiro Ito, Jai Prakash Singh, Shu-Fang Hsu, Alan Wells, Kuang-Yu Jen, Tzu-Ching Meng, Fawaz G. Haj
Summary: This study identified alpha-actinin4 as a novel substrate of PTP1B in podocytes and demonstrated their interaction in regulating podocyte function. Targeting PTP1B and alpha-actinin4 could be a potential therapeutic approach for podocyte injury.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Paulo F. V. Bizerra, Eduardo H. Gilglioni, Hang Lam Li, Simei Go, Ronald P. J. Oude Elferink, Arthur J. Verhoeven, Jung -Chin Chang
Summary: This study investigates the role of cyclic AMP (cAMP) in glycogen metabolism and reveals that cAMP regulates glycogenolysis in opposite directions depending on its site of synthesis within cells and downstream effectors. The canonical tmAC-cAMP-PKA signaling promotes glycogenolysis, while the non-canonical sAC-cAMP-Epac1 signaling suppresses glycogenolysis. This highlights the importance of cAMP microdomain organization for distinct metabolic regulation.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)