Article
Biotechnology & Applied Microbiology
Nicolas Barraud, Sylvie Letoffe, Christophe Beloin, Joelle Vinh, Giovanni Chiappetta, Jean-Marc Ghigo
Summary: The study compared cysteine S-nitrosylation in aerobic and anaerobic biofilm and planktonic Escherichia coli cultures, identifying proteins with biofilm-specific S-nitrosylation status. Impairing S-nitrosylation in proteins related to redox homeostasis and amino acid synthesis altered important biofilm properties, such as motility, biofilm maturation, and resistance to oxidative stress, indicating the physiological basis of E. coli adaptation to the biofilm environment.
NPJ BIOFILMS AND MICROBIOMES
(2021)
Article
Biochemistry & Molecular Biology
Federico Rossi, Cristina Catto, Gianmarco Mugnai, Federica Villa, Fabio Forlani
Summary: The study demonstrates the important role of WrbA in biofilm development, with its absence affecting biofilm formation and rendering the cells sensitive to reactive oxygen species. Salicylic and cinnamic acids act as antibiofilm agents by inhibiting WrbA's enzyme activity for the first time.
Article
Multidisciplinary Sciences
Phan Nguyen Trang, Tong Thi Anh Ngoc, Yoshimitsu Masuda, Ken-ichi Hohjoh, Takahisa Miyamoto
Summary: This study investigated the occurrence, antibiotic resistance, and biofilm formation of Escherichia coli in a Vietnamese fish processing facility. The findings showed that E. coli was most frequently detected in the wash water samples and Pangasius fish, with high resistance to sulfamethoxazole/trimethoprim and tetracycline. Some isolates exhibited multidrug resistance and strong biofilm forming capability. Additionally, acidic hypochlorous water and sodium hypochlorite were effective in reducing biofilm mass and cells of the isolates.
Article
Ecology
Peng Zhang, Daqing Mao, Huihui Gao, Liyang Zheng, Zeyou Chen, Yuting Gao, Yitao Duan, Jianhua Guo, Yi Luo, Hongqiang Ren
Summary: The study revealed that evolutionary adaptation of plasmid-carrying bacteria in an antibiotic-influenced environment facilitated colonization of the murine gut by the bacteria and transfer of plasmids to gut bacteria. The evolved strains exhibited phenotypic alterations, enhanced bacterial growth, biofilm formation capability, and decreased plasmid fitness cost, which were attributed to chromosomal mutations and transcriptional modifications. Upregulated genes, such as type 1 fimbrial-protein pilus and surface adhesin genes, were responsible for the enhanced biofilm-forming capacity, while efflux pump genes and plasmid-harboring genes showed differential transcriptional regulation.
Article
Infectious Diseases
Lachlan Jake Munro, Douglas B. Kell
Summary: Antibiotic resistance is a major global healthcare issue, and modification of membrane transporters is a key mechanism driving this resistance. This study demonstrates that transporters play a role in modulating the efficacy of almost all antimicrobial compounds tested, potentially influencing the development of antimicrobial resistance.
Article
Chemistry, Multidisciplinary
Shuting Fang, Yiquan Huang, Zhangmin Xiang, Rong Zeng, Shulin Zeng, Shuqin Liu
Summary: This study investigated the impact of polystyrene (PS) nanoparticles on the bacteriological properties of Escherichia coli O157:H7. The results showed that PS nanoparticles promoted the growth of E. coli O157:H7 and induced metabolic adaptation and changes in enzymatic activity. The study also found an increase in the expression of the AcrAB-TolC efflux pump, possibly leading to heightened resistance of E. coli O157:H7.
ENVIRONMENTAL SCIENCE-NANO
(2023)
Article
Environmental Sciences
Maria Celeste Pellegrini, Elena Okada, Ramon Alejandro Gonzalez Pasayo, Alejandra Graciela Ponce
Summary: This study characterized E. coli strains isolated from 10 horticultural farms, revealing that 67% of the isolates were resistant to amoxicillin-clavulanic acid and/or ampicillin, and all isolates had biofilm forming capacity. This is the first environmental assessment of resistant E. coli occurrence in horticultural farms in South America.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Xian Ju, Xingxing Fang, Yunzhu Xiao, Bingyu Li, Ruoping Shi, Chaoliang Wei, Conghui You
Summary: Small regulatory RNAs control bacterial activities, with the small RNA GcvB playing roles beyond amino acid metabolism. A study compared transcriptomes of wild-type E. coli and a gcvB deletion strain in minimal medium, identifying potential targets including the auto-aggregation gene flu. Further molecular studies confirmed GcvB's role in regulating oxidative stress response and upregulating OxyR expression, leading to increased sensitivity to oxidative stress in the gcvB knockout strain.
Article
Multidisciplinary Sciences
Yoshikazu Kawai, Maki Kawai, Eilidh Sohini Mackenzie, Yousef Dashti, Bernhard Kepplinger, Kevin John Waldron, Jeff Errington
Summary: Inhibition of bacterial cell wall synthesis by β-lactam antibiotics results in loss of cell wall integrity, leading to cell death through metabolic perturbations and oxidative damage. Kawai et al. identify key enzymatic steps that stimulate the generation of reactive oxygen species and highlight the role of iron homeostasis in the lethal effects mediated by oxidative damage.
NATURE COMMUNICATIONS
(2023)
Article
Environmental Sciences
Hongrui Guo, Zhicai Zuo, Fengyuan Wang, Caixia Gao, Kejie Chen, Jing Fang, Hengmin Cui, Ping Ouyang, Yi Geng, Zhengli Chen, Chao Huang, Yanqiu Zhu, Huidan Deng
Summary: The study investigated the relationship between obesity and heart health during non-fatal bacterial infection, finding that while infection led to cardiac inflammation, oxidative stress, and excessive apoptosis, obesity had a mitigating effect on these negative impacts.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2021)
Article
Biochemistry & Molecular Biology
Cheng-Hai Yan, Fang-Hui Chen, Yu-Lu Yang, Yu-Fan Zhan, Richard A. Herman, Lu-Chan Gong, Sheng Sheng, Jun Wang
Summary: This study successfully constructed a csgD deletion mutant Delta csgD using the CRISPR/Cas9 gene-editing system, and found that Delta csgD exhibited decreased biofilm formation, inhibited mobility and adhesion, and significantly decreased resistance to acid, alkali, and osmotic stress conditions.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Infectious Diseases
Anamaria Babosan, Margaux Gaschet, Anaelle Muggeo, Thomas Jove, David Skurnik, Marie-Cecile Ploy, Christophe de Champs, Fany Reffuveille, Thomas Guillard
Summary: Bacteria within biofilms exposed to sub-MIC concentrations of antibiotics can develop antibiotic resistance. This study shows that sub-MIC levels of aminoglycosides induce the SOS response in E. coli carrying the qnrD gene, leading to genetic rearrangements and increased biofilm formation. The presence of the qnrD-plasmid promotes biofilm production and the acquisition and spread of resistance determinants for other antibiotics.
Article
Biochemistry & Molecular Biology
Chong Li, Yang Li, Shuzhen Li, Si Chen, Guohua Liu, Xuejuan Deng, Wenhuan Chang, Huiyi Cai
Summary: Bacillus subtilis can improve the performance and health status of infected ducks, alleviate serum abnormalities caused by Escherichia coli infection, protect the intestine from damage, and regulate antioxidant and energy metabolism by activating ribosome and oxidative phosphorylation signaling.
Article
Chemistry, Organic
Jian Li, Hans Renata
Summary: A novel chemoenzymatic approach was developed for the synthesis of fasamycin A, a halogenated naphthacenoid with activities against methicillin-resistant Staphylococcus aureus and vancomycin-resistant Enterococcus faecalis. The synthesis involved combining two fragments through a Sammes annulation to form a dimethylnaphthacenone system, followed by enzymatic halogenation to introduce the necessary chlorine substituent at a later stage.
JOURNAL OF ORGANIC CHEMISTRY
(2021)
Article
Agriculture, Dairy & Animal Science
Mei Xue, Dandan Fu, Jiangang Hu, Ying Shao, Jian Tu, Xiangjun Song, Kezong Qi
Summary: This study investigated the effects of ygeK on biofilm formation and environmental stress resistance in Avian pathogenic Escherichia coli (APEC). The multi-level regulation of ygeK in APEC and associations between differentially expressed proteins and key ygeK targets were also analyzed. This research provides new insights into the pathogenicity of APEC and can contribute to the development of disease prevention and treatment strategies.
Article
Biotechnology & Applied Microbiology
Dorota Kuczynska-Wisnik, Maria Moruno-Algara, Karolina Stojowska-Swedrzynska, Ewa Laskowska
MICROBIAL CELL FACTORIES
(2016)
Article
Microbiology
Bozena Bruhn-Olszewska, Pawel Szczepaniak, Ewelina Matuszewska, Dorota Kuczynska-Wisnik, Karolina Stojowska-Swedrzynska, Maria Moruno Algara, Ewa Laskowska
MICROBIOLOGICAL RESEARCH
(2018)
Article
Biochemical Research Methods
Ewa Laskowska, Dorota Kuczynska-Wisnik, Barbara Lipinska
JOURNAL OF PROTEOMICS
(2019)
Article
Biochemistry & Molecular Biology
Maria Moruno Algara, Dorota Kuczynska-Wisnik, Janusz Debski, Karolina Stojowska-Swedrzynska, Hanna Sominka, Malgorzata Bukrejewska, Ewa Laskowska
MOLECULAR MICROBIOLOGY
(2019)
Review
Genetics & Heredity
Ewa Laskowska, Dorota Kuczynska-Wisnik
Article
Genetics & Heredity
Karolina Wilkowska, Iwona Mruk, Beata Furmanek-Blaszk, Marian Sektas
Review
Biochemistry & Molecular Biology
Soroosh Monem, Beata Furmanek-Blaszk, Adrianna Lupkowska, Dorota Kuczynska-Wisnik, Karolina Stojowska-Swedrzynska, Ewa Laskowska
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2020)
Article
Microbiology
Dorota Kuczynska-Wisnik, Chuanmin Cheng, Roman R. Ganta, Michal Zolkiewski
FEMS MICROBIOLOGY LETTERS
(2017)