Article
Biochemical Research Methods
Edyta Budzynska, Izabela Wolanska, Jaroslaw Puton
Summary: The presented work focuses on the impact of ionization mechanisms on detection sensitivity in negative-mode ion mobility spectrometry. The research was conducted on selected organic analytes using DT IMS in the negative mode of operation. Two ionization mechanisms, electron capture and ion-molecule reactions, can be used in the negative mode of detection depending on the carrier gas. The experiments were carried out using air and nitrogen as carrier gases, allowing for a comparison of the ionization mechanisms. The study also investigated the effect of oxygen and water admixtures in carrier gases on the detection efficiency of selected organic compounds.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2022)
Article
Biochemical Research Methods
Christian Ieritano, Daniel Rickert, Joshua Featherstone, John F. Honek, J. Larry Campbell, J. C. Yves Le Blanc, Bradley B. Schneider, W. Scott Hopkins
Summary: The study demonstrates that adding solvent vapor to DMS can stabilize protonated peptides, reduce collision-induced fragmentation, and lower temperatures. Additionally, the solvation propensity of solvents aids in stabilizing charge density.
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
(2021)
Article
Chemistry, Analytical
Junhui Li, Lei Li, Wenqing Gao, Shoudong Shi, Jiancheng Yu, Keqi Tang
Summary: A high-performance FAIMS-IMS-MS platform was developed for exploring the conformational diversity of bradykinin. The FAIMS-IMS technique can resolve multiple conformers of (BK + H+) ions, and the separations in FAIMS and IMS dimensions are largely orthogonal, resulting in a higher overall resolving power.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Analytical
Kelly L. Wormwood Moser, Gregory Van Aken, Daniel DeBord, Nathan Galen Hatcher, Laura Maxon, Melissa Sherman, Lihang Yao, Kim Ekroos
Summary: Defects in sphingolipid metabolism serve as a common link in various neurodegenerative disorders, and a better understanding of lipid content presents opportunities for the development of new therapeutic targets and biomarkers.
ANALYTICA CHIMICA ACTA
(2021)
Article
Biochemical Research Methods
Melanie Cheung See Kit, Samantha O. Shepherd, James S. Prell, Ian K. Webb
Summary: By utilizing Monte Carlo simulations of ion temperatures within ion guides, this study characterizes the energetic properties of long-lived complexes formed through ion/ion reactions. Covalent bond formation is found to be more favorable than unfolding or bond cleavage, with activation energies between 55 to 75 kJ/mol accessible through moderate collisional activation. Bond formation is favored at lower energies, while reagent loss becomes competitive at higher collision energies.
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
(2021)
Article
Chemistry, Analytical
Aurelie Delvaux, Estelle Rathahao-Paris, Blanche Guillon, Sophie Cholet, Karine Adel-Patient, Francois Fenaille, Christophe Junot, Sandra Alves
Summary: The study introduces a high throughput and high resolution method for characterizing main oligosaccharides in breastmilk using ion mobility spectrometry, offering high identification level. Standard HMO isomers were investigated and could be distinguished effectively. Multiple mobility peaks were observed for some HMO species, but reduction at the reducing-end did not significantly improve isomer distinction.
ANALYTICA CHIMICA ACTA
(2021)
Article
Biochemical Research Methods
Robert P. Pellegrinelli, Lei Yue, Eduardo Carrascosa, Ahmed Ben Faleh, Stephan Warnke, Priyanka Bansal, Thomas R. Rizzo
Summary: This article presents a systematic method of identifying reducing-end anomers of glycans using ultrahigh-resolution ion mobility, collision-induced dissociation, and cryogenic infrared spectroscopy. The results confirm that the infrared spectra can rapidly identify precursor glycans with a single anomer.
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
(2022)
Article
Multidisciplinary Sciences
Xiaoyu Zhou, Zhuofan Wang, Jingjin Fan, Zheng Ouyang
Summary: Ion mobility is utilized in mass spectrometers for analyzing the structure of biomolecules. Researchers demonstrate that ion mobility analysis in an ion trap with ultra-high fields can achieve resolutions over 10,000 for isomer separation, as shown for disaccharide, phospholipid, and peptide isomers. Elucidating complex structures of biomolecules is crucial in chemistry and life sciences. In the last decade, ion mobility coupled with mass spectrometry has become a unique tool for distinguishing isomers and isoforms of biomolecules. This study presents a concept for ion mobility analysis using an ion trap, enabling ultra-high field isomer separation and achieving resolutions over 10,000. The potential of this technology is demonstrated for analyzing isomers of disaccharides, phospholipids, and peptides with post-translational modifications.
NATURE COMMUNICATIONS
(2023)
Article
Chemistry, Multidisciplinary
Karen E. Butler, Yoshihiko Takinami, Adam Rainczuk, Erin S. Baker, Blaine R. Roberts
Summary: Native mass spectrometry combined with IMS-MS technology can be used to study the tertiary and quaternary structure of proteins, validate other structural determination methods, and provide more comprehensive structural information.
FRONTIERS IN CHEMISTRY
(2021)
Review
Chemistry, Analytical
Daniel G. Delafield, Gaoyuan Lu, Cameron J. Kaminsky, Lingjun Li
Summary: Mass spectrometry-based biomolecular analyses have become popular in research settings. Gas phase ion separation techniques provide a fourth dimension of analysis, enabling rapid characterization and high throughput profiling. This review highlights the latest developments and applications of high-end ion-mobility enabled instruments and their advantages for biomolecular research.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Multidisciplinary
David S. Roberts, Morgan Mann, Jake A. Melby, Eli J. Larson, Yanlong Zhu, Allan R. Brasier, Song Jin, Ying Ge
Summary: Researchers have successfully elucidated the complete structure of newly discovered O-glycans on the S protein regional-binding domain (SR-BD) of the SARS-CoV-2 virus through a hybrid mass spectrometry approach. This method can provide insights into the interactions between the virus and host cells, laying a foundation for understanding the functional roles of O-glycans in SARS-CoV-2 and other glycoproteins.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2021)
Review
Spectroscopy
Theresa A. Gozzo, Matthew F. Bush
Summary: Collision cross-section values of protein ions, which can be determined by ion mobility experiments, are sensitive to the ions' structures and have applications in structural biology and biophysics. An extensive understanding of the relationship between charge state and collision cross-section values is still lacking. Here, we review cation-to-anion, proton-transfer reactions (CAPTR), a method that generates a series of charge-reduced protein cations by reacting quadrupole-selected cations with even-electron monoanions. The resulting CAPTR products are analyzed using ion mobility, mass spectrometry, and collisional activation, providing insights into the relationship between protein ion structure and charge state.
MASS SPECTROMETRY REVIEWS
(2023)
Article
Biochemistry & Molecular Biology
Wojciech Fabianowski, Miroslaw Maziejuk, Monika Szyposzynska, Monika Wisnik-Sawka
Summary: The article presents a technique of differential ion mobility spectrometry (DMS) for the detection and identification of volatile organic compounds (VOCs) from various categories. It analyzes the possibility of mixture identification using only the DMS spectrometer and provides several examples. The article also discusses the analysis of different compounds, their mechanisms of fragmentation, influence on effective ion temperature, and high electric field intensity.
Article
Agriculture, Multidisciplinary
Xue-Chao Song, Elena Canellas, Nicola Dreolin, Cristina Nerin, Jeff Goshawk
Summary: The study characterized and quantified phenolic compounds in bearberry leaves using IMS and a mass spectrometer, identifying 88 compounds including arbutin, tannins, flavanols, and flavonols. Arbutin, 1,2,3,4,6-pentagalloylglucose, and quercetin were the most abundant phenolic components in the leaves.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2021)
Article
Biochemistry & Molecular Biology
Izabela Wolanska, Edyta Budzynska, Jaroslaw Puton
Summary: This study investigates the ionization process of benzyl chloride in a drift tube ion mobility spectrometer using nitrogen as the carrier gas, revealing that ionization follows the dissociative electron capture mechanism and leads to the formation of cluster ions. By analyzing drift time spectra, the electron attachment rate for BzCl and the equilibrium constant for cluster ion formation were estimated. Theoretical interpretation of the results is based on ion transport mathematically, considering diffusion, formation, and dissociation of ionic clusters during ion movement in the drift section.
Article
Engineering, Environmental
Sergio Montoya-Suarez, Fredy Colpas-Castillo, Edgardo Meza-Fuentes, Johana Rodriguez-Ruiz, Roberto Fernandez-Maestre
WATER SCIENCE AND TECHNOLOGY
(2016)
Article
Physics, Atomic, Molecular & Chemical
Roberto Fernandez-Maestre, Dairo Meza-Morelos, Ching Wu
INTERNATIONAL JOURNAL OF MASS SPECTROMETRY
(2016)
Article
Biochemical Research Methods
Roberto Fernandez-Maestre, Dairo Meza-Morelos, Ching Wu
RAPID COMMUNICATIONS IN MASS SPECTROMETRY
(2016)
Article
Physics, Atomic, Molecular & Chemical
Roberto Fernandez-Maestre
INTERNATIONAL JOURNAL OF MASS SPECTROMETRY
(2017)
Article
Biochemical Research Methods
Dairo Meza-Morelos, Roberto Fernandez-Maestre
JOURNAL OF MASS SPECTROMETRY
(2017)
Article
Instruments & Instrumentation
R. Fernandez-Maestre
REVIEW OF SCIENTIFIC INSTRUMENTS
(2017)
Article
Environmental Sciences
Roberto Fernandez-Maestre, Boris Johnson-Restrepo, Jesus Olivero-Verbel
INTERNATIONAL JOURNAL OF ENVIRONMENTAL RESEARCH
(2018)
Review
Biochemical Research Methods
Roberto Fernandez-Maestre
JOURNAL OF MASS SPECTROMETRY
(2018)
Article
Biochemical Research Methods
Roberto Fernandez-Maestre, Mahmoud Tabrizchi, Dairo Meza-Morelos
Summary: This study investigated the effects of interaction energy, intermolecular bonds, and analyte size on ion mobility shifts by introducing different shift reagents and using electrospray ionization-IMS-mass spectrometry. The results showed that shift reagents can effectively discriminate false positives caused by overlapping peaks, which is important for resolving interferences and improving the accuracy of detection methods.
RAPID COMMUNICATIONS IN MASS SPECTROMETRY
(2022)
Article
Chemistry, Analytical
Roberto Fernandez-Maestre, Markus Doerr
Summary: Drift tube ion mobility spectrometry (IMS)-quadrupole mass spectrometry (QMS) was used to test the separation of enantiomers in racemic mixtures of twelve alpha-amino acids and three chiral drugs. Chiral selectors were introduced into the buffer gas of the IMS instrument. However, the racemic mixtures could not be separated, and several explanations were analyzed. Recommendations for potential enantiomer separations by IMS were proposed.
ANALYTICAL METHODS
(2022)
Article
Chemistry, Analytical
Roberto Fernandez-Maestre, Martha C. Daza
Summary: The study investigated the effect of 2-butanol as a contaminant in ion mobility spectrometry (IMS) and found that at different temperatures, the addition of 2-butanol influenced the ion mobilities of amino acid substances, with the most significant impact occurring at lower temperatures. Therefore, for accurate reduced mobilities, it is recommended to conduct drift-tube ion mobility measurements at high temperatures.
ANALYTICAL METHODS
(2021)
Article
Chemistry, Analytical
Roberto Fernandez Maestre
ANALYTICAL METHODS
(2017)
Article
Spectroscopy
Roberto Fernandez-Maestre
INTERNATIONAL JOURNAL FOR ION MOBILITY SPECTROMETRY
(2017)
Article
Spectroscopy
Dairo Meza-Morelos, Roberto Fernandez-Maestre, Herbert H. Hill
INTERNATIONAL JOURNAL FOR ION MOBILITY SPECTROMETRY
(2016)
Review
Medicine, General & Internal
Katiuska I. Romero, Roberto Fernandez-Maestre
REVISTA DA ASSOCIACAO MEDICA BRASILEIRA
(2018)