Review
Plant Sciences
Mattia Terzaghi, Mario C. De Tullio
Summary: This article summarizes the various attempts made to improve AsA production/content in plants, highlighting the most promising ones, and providing information about the possible unexpected consequences of a purely biotechnological approach.
FRONTIERS IN PLANT SCIENCE
(2022)
Review
Biochemistry & Molecular Biology
Sabrina L. Slater, Despoina A. I. Mavridou
Summary: Protein folding is crucial for biological function and recombinant protein production, and the correct formation of disulfide bonds is essential for protein stability. In bacterial expression systems, the limitations of endogenous posttranslational modification systems can hinder the production of proteins with their native folds.
MOLECULAR MICROBIOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Laura Navone, Thomas Vogl, Pawarisa Luangthongkam, Jo-Anne Blinco, Carlos H. Luna-Flores, Xiaojing Chen, Juhani von Hellens, Stephen Mahler, Robert Speight
Summary: This study presents a thermostable variant of the E. coli AppA phytase, ApV1, with an extra non-consecutive disulfide bond, which shows promising thermostability but leads to decreased production in Pichia pastoris. Co-expression of folding chaperones like protein disulfide bond isomerase (Pdi) significantly increased the production of ApV1 and restored yields to levels comparable with the wild-type enzyme. Engineering for enhanced enzymatic properties may result in folding complexity and reduced production in microbial systems, highlighting the importance of developing improved production strains in parallel to achieve desirable levels of recombinant protein for industrial processes.
BIOTECHNOLOGY FOR BIOFUELS
(2021)
Review
Biochemistry & Molecular Biology
Jiahui Fu, Jihui Gao, Zhongxin Liang, Dong Yang
Summary: Disulfide bonds are crucial for maintaining protein structures and biological functions, with PDIs playing a key role in regulating their formation.
Article
Biochemistry & Molecular Biology
Shunsuke Okada, Motonori Matsusaki, Masaki Okumura, Takahiro Muraoka
Summary: This study investigated the influence of a spacer between thiol and guanidyl units on oxidative protein folding, revealing that a conjugate with a diethylene glycol spacer showed lower folding promotion effect. The reduced efficiency was likely due to the lower acidity and more reductive property of the thiol group compared to the conjugate without the spacer. This suggests that the spacer between thiol and guanidyl groups plays a critical role in promoting oxidative protein folding.
Article
Food Science & Technology
Akiko Isomoto, Eiichi Shoguchi, Kanako Hisata, Jun Inoue, Yinrui Sun, Kenji Inaba, Noriyuki Satoh, Tomohisa Ogawa, Hiroki Shibata
Summary: This study identified genes involved in the modification and functioning of venom proteins in Protobothrops flavoviridis, a venomous snake species. Genes encoding protein disulfide isomerase (PDI) family members, Selenoprotein M (SELENOM), and Calreticulin (CALR) were highly expressed in venom glands, suggesting their potential role in protein folding and modification.
Article
Biology
Motonori Matsusaki, Rina Okada, Yuya Tanikawa, Shingo Kanemura, Dai Ito, Yuxi Lin, Mai Watabe, Hiroshi Yamaguchi, Tomohide Saio, Young-Ho Lee, Kenji Inaba, Masaki Okumura
Summary: The physiological functions of proteins are determined by their unique three-dimensional structures, with conserved disulfide-catalysts and chaperone networks playing a crucial role in correct protein folding and prevention of aggregation. Disruption of these networks is implicated in pathology. Complex formation among PDIs accelerates protein folding and prevents aggregation.
Article
Biochemistry & Molecular Biology
Young-Saeng Kim, Seong-Im Park, Jin-Ju Kim, Sun-Young Shin, Sang-Soo Kwak, Choon-Hwan Lee, Hyang-Mi Park, Yul-Ho Kim, Il-Sup Kim, Ho-Sung Yoon
Summary: Abiotic stress can induce the generation of reactive oxygen species (ROS) in plants, leading to oxidative damage. In this study, we developed salt-tolerant transgenic rice plants that overexpressed the OsDHAR1 gene under the control of a stress-inducible promoter. These transgenic plants showed improved environmental adaptability and agronomic traits, including root development and grain yield. Knockdown of the OsDHAR2 gene in plants resulted in sensitivity to salt stress and reduced biomass and grain yield. Microarray analysis revealed the upregulation of genes related to cell growth, plant growth, senescence, and stress response in the transgenic plants. Our findings provide insights into the physiological processes underlying environmental adaptability and have implications for crop breeding and climate change adaptation.
Article
Biochemistry & Molecular Biology
Akane Hamada, Takahiro Ishikawa, Takanori Maruta
Summary: This study investigates the impact of ascorbate recycling capacity on ascorbate pool size in an ascorbate-deficient background. The findings demonstrate that a smaller ascorbate pool size lowers the need for ascorbate recycling capacity even under light stress.
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
(2023)
Article
Plant Sciences
Jie-Xia Liu, Hao Wang, Kai Feng, Tong Li, Yan-Hua Liu, Ao-Qi Duan, Sheng Shu, Hui Liu, Ai-Sheng Xiong
Summary: This study identified a gene, AgDHAR2, encoding dehydroascorbate reductase from celery, which plays a role in AsA accumulation and drought stress response. Overexpression of AgDHAR2 in Arabidopsis and celery plants led to increased AsA contents and improved drought tolerance by enhancing antioxidant-related genes expression and maintaining redox state. This indicates that AgDHAR2 may contribute to enhanced drought tolerance in celery by increasing AsA concentrations.
ENVIRONMENTAL AND EXPERIMENTAL BOTANY
(2022)
Article
Plant Sciences
Akane Hamada, Yasuhiro Tanaka, Takahiro Ishikawa, Takanori Maruta
Summary: Ascorbate is an essential antioxidant for plant growth and stress tolerance. Recent research shows that chloroplastic DHAR3, in collaboration with glutathione, plays a key role in accumulating ascorbate under high-light stress. This study highlights the importance of balancing stress intensity and recycling capacity for maintaining ascorbate pool size and tolerance to photooxidative stress.
Article
Biotechnology & Applied Microbiology
Laura Navone, Thomas Vogl, Pawarisa Luangthongkam, Jo-Anne Blinco, Carlos Luna-Flores, Xiaojing Chen, Juhani von Hellens, Robert Speight
Summary: The study explores potential bottlenecks in expressing E. coli AppA phytase in P. pastoris by using bidirectional promoters (BDPs) and other proteins. Transcriptional studies provide insights into the expression profile of BDPs. The use of ERV2, a flavoprotein not previously characterized in P. pastoris, improves phytase expression, suggesting it as an alternative pathway to ERO1.
MICROBIAL CELL FACTORIES
(2021)
Article
Biotechnology & Applied Microbiology
Lukas A. A. Rettenbacher, Tobias von der Haar
Summary: This study establishes a novel approach for quantitatively investigating the ability of E. coli to produce disulfide bonds in its proteome. The study shows that the demand for disulfide bonded proteins in E. coli changes significantly under different growth conditions, and predicts under what conditions excess capability is available for recombinant protein production.
MICROBIAL CELL FACTORIES
(2022)
Article
Biochemistry & Molecular Biology
Lei Zhu, Hongxin Zhao, Juanjuan Liu, Hao Cai, Bo Wu, Zhijun Liu, Shu Zhou, Qingsong Liu, Xiaokun Li, Bin Bao, Jian Liu, Han Dai, Junfeng Wang
Summary: FGF21 is a regulator of glucose and lipid metabolism, and the designed FGF21(SS) chimera shows improved thermostability and effectiveness against T2DM through structural analysis and design.
Article
Biochemistry & Molecular Biology
Mariana H. Moreira, Fabio C. L. Almeida, Tatiana Domitrovic, Fernando L. Palhano
Summary: Defensins are small, amphipathic proteins with disulfide bonds, challenging tertiary protein structure predictors. Comparing defensins with non-defensins showed no differences in residue proportions and solvent exposure. Bonded small proteins had more exposed apolar residues than unbonded proteins, with Robetta predicting unbonded proteins more accurately. trRosetta, using AI, improved prediction for bonded proteins but not unbonded ones, highlighting the complexity of predicting protein tertiary structures.
COMPUTATIONAL AND STRUCTURAL BIOTECHNOLOGY JOURNAL
(2021)
Article
Oncology
Tibor Balogh, Tamas Lorincz, Ibolya Stiller, Jozsef Mandl, Gabor Banhegyi, Andras Szarka
PATHOLOGY & ONCOLOGY RESEARCH
(2016)
Article
Oncology
Tamas Lorincz, Katalin Jemnitz, Tamas Kardon, Jzsef Mandl, Andras Szarka
PATHOLOGY & ONCOLOGY RESEARCH
(2015)
Article
Pharmacology & Pharmacy
Tamas Lorincz, Andras Szarka
JOURNAL OF PHARMACOLOGICAL AND TOXICOLOGICAL METHODS
(2017)
Article
Engineering, Chemical
Peter Hajdinak, Adam Czobor, Tamas Lorincz, Andras Szarka
PERIODICA POLYTECHNICA-CHEMICAL ENGINEERING
(2019)
Article
Engineering, Chemical
Tamas Lorincz, Andras Szarka
PERIODICA POLYTECHNICA-CHEMICAL ENGINEERING
(2018)
Review
Biochemistry & Molecular Biology
Szilvia Z. Toth, Tamas Lorincz, Andras Szarka
ANTIOXIDANTS & REDOX SIGNALING
(2018)
Article
Biochemistry & Molecular Biology
Monika Kosztelnik, Anita Kurucz, Diana Papp, Emily Jones, Timea Sigmond, Janos Barna, Maria H. Traka, Tamas Lorincz, Andras Szarka, Gabor Banhegyi, Tibor Vellai, Tamas Korcsmaros, Orsolya Kapuy
Article
Oncology
Tamas Lorincz, Marianna Holczer, Orsolya Kapuy, Andras Szarka
PATHOLOGY & ONCOLOGY RESEARCH
(2019)
Article
Biochemistry & Molecular Biology
Marianna Holczer, Bence Hajdu, Tamas Lorincz, Andras Szarka, Gabor Banhegyi, Orsolya Kapuy
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2019)
Article
Biochemistry & Molecular Biology
Andras Szarka, Orsolya Kapuy, Tamas Lorincz, Gabor Banhegyi
Summary: Oxidative stress caused by ascorbate can be a significant strategy to eliminate cancer cells selectively, with different concentrations inducing various cell death pathways. Understanding the mechanisms underlying high-dose ascorbate-induced cancer cell death may provide insights for synergistic cancer therapies in the future.
ANTIOXIDANTS & REDOX SIGNALING
(2021)
Article
Multidisciplinary Sciences
Marianna Holczer, Bence Hajdu, Tamas Lorincz, Andras Szarka, Gabor Banhegyi, Orsolya Kapuy
SCIENTIFIC REPORTS
(2020)
Article
Biology
Tamas Lorincz, Veronika Deak, Kinga Makk-Merczel, Dora Varga, Peter Hajdinak, Andras Szarka
Summary: The study compared HepG2 and HepaRG cell lines in terms of acetaminophen-induced hepatotoxicity, finding differences in activated pathways. While 3D culture could elevate CYP2E1 mRNA levels, it did not significantly increase hepatocyte function. HepaRG cells exhibited intermediate properties between HepG2 and primary hepatocytes, with relative immortality, stable phenotype, and CYP450 expression.
Meeting Abstract
Biochemistry & Molecular Biology
Tamas Lorincz, Andras Szarka
FREE RADICAL BIOLOGY AND MEDICINE
(2021)
Meeting Abstract
Biochemistry & Molecular Biology
Tamas Lorincz, Veronika Deak, Kinga Makk-Merczel, Dora Varga, Andras Szarka
FREE RADICAL BIOLOGY AND MEDICINE
(2021)
Review
Biochemistry & Molecular Biology
Andras Szarka, Tamas Lorincz, Peter Hajdinak
Summary: Iron, reactive oxygen species (ROS), and ascorbate play dual roles in cell fate decision, and their interactions have significant impacts on cell death.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)