期刊
PROTEOMICS
卷 14, 期 2-3, 页码 169-180出版社
WILEY
DOI: 10.1002/pmic.201300352
关键词
Bioinformatics; MSE; MS1 filtering; Proteomic quantitation; Skyline; Small molecule quantitation
资金
- National Natural Science Foundation of China [31170782]
- Tianjin Natural Science Foundation [11JCYBJC25500]
- Ph.D. Programs Foundation of Ministry of Education of China [20110031120019]
- National Biomedical Special Project of International Innovation Park [10ZCKFSY07200]
The MSE (where MSE is low energy (MS) and elevated energy (E) mode of acquisition) acquisition method commercialized by Waters on its Q-TOF instruments is regarded as a unique data-independent fragmentation approach that improves the accuracy and dynamic range of label-free proteomic quantitation. Due to its special format, MSE acquisition files cannot be independently analyzed with most widely used open-source proteomic software specialized for processing data-dependent acquisition files. In this study, we established a workflow integrating Skyline, a popular and versatile peptide-centric quantitation program, and a statistical tool DiffProt to fulfill MSE-based proteomic quantitation. Comparison with the vendor software package for analyzing targeted phosphopeptides and global proteomic datasets reveals distinct advantages of Skyline in MSE data mining, including sensitive peak detection, flexible peptide filtering, and transparent step-by-step workflow. Moreover, we developed a new procedure such that Skyline MS1 filtering was extended to small molecule quantitation for the first time. This new utility of Skyline was examined in a protein-ligand interaction experiment to identify multiple chemical compounds specifically bound to NDM-1 (where NDM is New Delhi metallo--lactamase 1), an antibiotics-resistance target. Further improvement of the current weaknesses in Skyline MS1 filtering is expected to enhance the reliability of this powerful program in full scan-based quantitation of both peptides and small molecules.
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